LOCUS       Exported               10000 bp ds-DNA    circular SYN 01-12-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    20181118_JH81118B_pLenti-NF-kBRp-Cre-T2A-mRuby3
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   jihye
  TITLE     Direct Submission
  JOURNAL   Exported 2020¤¤¤Å¤¤ 12 1¤·¤Ó¤© ¤¾¤È¤·¤Ë¤·¤Ó¤© from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10000
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        238..617
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2440..2557
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2641..2651
                     /note="NF-kB motif1"
     misc_feature    2652..2661
                     /note="NF-kB motif2"
     misc_feature    2662..2672
                     /note="NF-kB motif1"
     misc_feature    2673..2682
                     /note="NF-kB motif2"
     misc_feature    2683..2692
                     /note="NF-kB motif2"
     misc_feature    2693..2703
                     /note="NF-kB motif1"
     misc_feature    2704..2713
                     /note="NF-kB motif2"
     misc_feature    2714..2724
                     /note="NF-kB motif1"
     misc_feature    2725..2734
                     /note="NF-kB motif2"
     misc_feature    2851..3897
                     /note="Cre"
     CDS             2851..2871
                     /codon_start=1
                     /locus_tag="
                     "
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /note="
                     "
                     /protein_id="
                     "
                     /translation="PKKKRKV"
     CDS             3898..3924
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     CDS             3940..3993
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /protein_id="
                     "
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    3994..4707
                     /note="mRuby3"
     misc_feature    4732..5320
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5203..5214)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     primer_bind     complement(5323..5339)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             5845..6025
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6057..6281
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6327..6755
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6769..7058
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      6942..7044
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        7106..7153
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     CDS             7172..7546
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7676..7797
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7846..7862)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7870..7886
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7894..7924)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7939..7960
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8248..8836)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9007..9867)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9868..9972)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagacagg tgccagaaca tttctctatc gataggtacc
     2641 ggggactttc cgggaatttc cggggacttt ccgggaattt ccgggaattt ccggggactt
     2701 tccgggaatt tccggggact ttccgggaat ttccagatct ggcctcggcg gccaagcttg
     2761 ctagcggggg gctataaaag ggggtggggg cgttcgtcct cactctagtt ctgcgatcta
     2821 agtaagcttg gcattaccgg tgccaccatg cccaagaaga agaggaaggt gtccaatctc
     2881 ctgactgttc accagaacct ccctgcgctg ccagtagatg ccactagcga tgaggtcagg
     2941 aaaaatctca tggatatgtt tagggataga caggcgtttt ctgaacacac ctggaaaatg
     3001 ctgcttagcg tgtgccgatc ctgggcagcc tggtgtaagc tgaacaatcg caaatggttc
     3061 cccgccgagc cggaggacgt gcgcgattac ctgctgtatc tccaggcaag agggctggct
     3121 gtcaagacta tccagcagca cttgggccaa ctgaatatgc tgcatcgacg cagcgggctc
     3181 ccccggccta gcgattcaaa cgcagtctcc cttgttatga ggagaattag aaaggaaaac
     3241 gtagatgcgg gtgagagggc taagcaggct ctcgcttttg agcggactga tttcgaccag
     3301 gtcagatccc tgatggagaa cagcgatcgg tgccaggaca tcaggaacct cgcatttctg
     3361 ggaattgcat ataacacact tctgcgcata gctgagatcg cccggatcag agtgaaagac
     3421 atcagtcgaa cggacggcgg ccggatgctt attcatattg gacgcacaaa gacattggtc
     3481 agcaccgctg gcgttgaaaa ggccttgtcc ctgggcgtaa cgaagctggt ggaaagatgg
     3541 atctcagtgt ccggcgtggc tgacgaccct aataattact tgttctgtcg agtgagaaaa
     3601 aacggagtcg ccgcgccctc tgccaccagc caattgagta cacgggccct tgaagggatc
     3661 tttgaggcaa cccaccgact catatacgga gccaaggatg acagtggcca gaggtatctc
     3721 gcctggtcag gtcattctgc tagggtgggg gccgcacgag acatggcgcg ggcaggagtc
     3781 tccataccag agattatgca agctggaggt tggacaaatg tgaacatcgt tatgaactat
     3841 atccgcaatc ttgactctga aaccggggcc atggtgagac tgctcgaaga tggtgactac
     3901 ccatacgatg ttccagatta cgctggatcc ggcagtggag agggcagagg aagtctgcta
     3961 acatgcggtg acgtcgagga gaatcctggc ccaatggtgt ctaagggcga agagctgatc
     4021 aaggaaaata tgcgtatgaa ggtggtcatg gaaggttcgg tcaacggcca ccaattcaaa
     4081 tgcacaggtg aaggagaagg cagaccgtac gagggaactc aaaccatgag gatcaaagtc
     4141 atcgagggag gacccctgcc atttgccttt gacattcttg ccacgtcgtt catgtatggc
     4201 agccgtactt ttatcaagta cccggccgac atccctgatt tctttaaaca gtcctttcct
     4261 gagggtttta cttgggaaag agttacgaga tacgaagatg gtggagtcgt caccgtcacg
     4321 caggacacca gccttgagga tggcgagctc gtctacaacg tcaaggtcag aggggtaaac
     4381 tttccctcca atggtcccgt gatgcagaag aagaccaagg gttgggagcc taatacagag
     4441 atgatgtatc cagcagatgg tggtctgaga ggatacactg acatcgcact gaaagttgat
     4501 ggtggtggcc atctgcactg caacttcgtg acaacttaca ggtcaaaaaa gaccgtcggg
     4561 aacatcaaga tgcccggtgt ccatgccgtt gatcaccgcc tggaaaggat cgaggagagt
     4621 gacaatgaaa cctacgtagt gcaacgcgaa gtggcagttg ccaaatacag caaccttggt
     4681 ggtggcatgg acgagctgta caagtaagaa ttcgatatca agcttatcgg taatcaacct
     4741 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4801 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4861 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4921 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4981 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5041 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5101 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     5161 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5221 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     5281 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     5341 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     5401 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     5461 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5521 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     5581 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     5641 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     5701 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     5761 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     5821 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     5881 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5941 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6001 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     6061 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     6121 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     6181 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     6241 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     6301 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     6361 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6421 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6481 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6541 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6601 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     6661 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     6721 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     6781 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6841 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6901 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctctccgccc catggctgac
     6961 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     7021 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     7081 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     7141 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     7201 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     7261 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     7321 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     7381 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     7441 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     7501 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     7561 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     7621 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     7681 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7741 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7801 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     7861 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7921 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7981 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8041 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8101 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8161 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8221 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8281 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8341 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8401 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8461 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8521 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8581 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8641 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8701 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8761 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8821 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8881 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8941 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9001 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9061 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9121 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9181 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9241 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9301 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9361 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9421 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9481 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9541 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9601 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9661 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9721 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9781 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9841 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9901 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9961 aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
