LOCUS       Exported               12370 bp ds-DNA    circular SYN 01-12-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    Cre-responsive Cas9 expressing transposon library vector
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12370)
  AUTHORS   jihye
  TITLE     Direct Submission
  JOURNAL   Exported 2020¤¤¤Å¤¤ 12 1¤·¤Ó¤© ¤¾¤È¤·¤Ë¤·¤Ó¤© from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12370
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     23..39
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    48..81
                     /note="ITR(L)"
     misc_feature    59..76
                     /note="ITR consensus"
     misc_feature    118..304
                     /note="insulator"
     polyA_signal    311..535
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    542..562
                     /note="Reverse primer (GOI specific, from Church)"
     protein_bind    563..596
                     /bound_moiety="Cre recombinase"
                     /note="lox2272"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     CDS             complement(624..1223)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     protein_bind    1269..1302
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        complement(1309..2486)
                     /note="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1318..2255
                     /note="EF-1-alpha intron A"
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     promoter        complement(2614..2854)
                     /note="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     protein_bind    complement(2866..2899)
                     /bound_moiety="Cre recombinase"
                     /note="lox2272"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     polyA_signal    3086..3220
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     polyA_signal    3340..3474
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     polyA_signal    3594..3728
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3788..3821)
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             3837..7940
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /note="Cas9"
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7956..7976
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             7977..8003
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     CDS             8019..8078
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /note="E2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             8085..8801
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /note="Clover"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     polyA_signal    8856..8937
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    9244..9430
                     /note="insulator"
     misc_feature    9513..9544
                     /note="ITR(R)"
     misc_feature    9518..9535
                     /note="ITR consensus"
     promoter        complement(9605..9623)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9644..9660)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9668..9684
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9692..9722)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9737..9758
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10046..10634)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10805..11665)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11666..11770)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      11797..12252
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gggttttccc agtcacgacg ttgtaaaacg acggccagtg gcgcgcctac agttgaagtc
       61 ggaagtttac atacacttaa ggacaggatt ggtgacagaa aagccccatc cttaggcagc
      121 tagtcaggta gtaaaaggtc tcaactaggc agtggcagag caggattcaa attcagggct
      181 gttgtgatgc ctccgcagac tctgagcgcc acctggtggt aatttgtctg tgcctcttct
      241 gacgtggaag aacagcaact aacacactaa cacggcattt actatgggcc agccattgtt
      301 ctaagctagc ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
      361 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
      421 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
      481 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggggtac
      541 ctcgtatgcc gtcttctgct tgataacttc gtataggata ctttatacga agttataact
      601 tacttgtaca gctcgtaacg cgttcaggca ccgggcttgc gggtcatgca ccaggtgcgc
      661 ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc cgagccgctc gtagaagggg
      721 aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc
      781 actccgggga gcacgacggc gctgcccaga cccttgccct ggtggtcggg cgagactccg
      841 acggtggcca ggaaccacgc gggctccttg ggccggtgcg gcgccaggag gccttccatc
      901 tgttgctgcg cggccagccg ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg
      961 aacaccgccc ccgcttcgac gctctccggc gtggtccaga ccgccacggc ggcgccgtcg
     1021 tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc
     1081 tcggtgaccc gctcgatgtg gcggtccgga tcgacggtgt ggcgcgtggc ggggtagtcg
     1141 gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt cgtcgcgggt ggcgaggcgc
     1201 accgtgggct tgtactcggt catggtggcg atatcatgta agttgtcacg aagttgcagt
     1261 gcagatggat aacttcgtat agcatacatt atacgaagtt atcgtacgtc acgacacctg
     1321 aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc aagatccaaa
     1381 ctcaaaaagg gcaaattcca aggagaatta catcaagtgc caagctggcc taacttcagt
     1441 ctccacccac tcagtgtggg gaaactccat cgcataaaac ccctcccccc aacctgaaga
     1501 cgacgtactc caaaagctcg agaactaatc gaggtgcctg gacggcgccc ggtactccgt
     1561 ggagtcacat gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg tgggtgactc
     1621 acccgcccgc tctcccgagc gccgcgtcct ccattttgag ctccctgcag cagggccggg
     1681 aagcggccat ctttccgctc acgcaactgg tgccgaccgg gccagccttg ccgcccaggg
     1741 cggggcgata cacggcggcg cgaggccagg caccagagca ggccggccag cttgagacta
     1801 cccccgtccg attctcggtg gccgcgctcg caggccccgc ctcgccgaac atgtgcgctg
     1861 ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag tgtgcagatc
     1921 ttggcccgca tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag gtcatcaaaa
     1981 attttaaatg gctagagact tatcgaaagc agcgagacag gcgcgaaggt gccaccagat
     2041 tcgcacgcgg cggccccagc gcccaggcca ggcctcaact caagcacgag gcgaaggggc
     2101 tccttaagcg caaggcctcg aactctccca cccacttcca acccgaagct cgggatcaag
     2161 aatcacgtac tgcagccagt ggaagtaatt caaggcacgc aagggccata acccgtaaag
     2221 aggccaggcc cgcgggaacc acacacggca cttacctgtg ttctggcggc aaacccgttg
     2281 cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc accctcggga
     2341 aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca ccttctctag
     2401 gcaccggatc aattgccgac ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc
     2461 tctgcccact gacgggcacc ggagccttaa aaggcgcgtc tagaataaaa aaagcaccga
     2521 ctcggtgcca ctttttcaag ttgataacgg actagcctta ggttaacttg ctatttctag
     2581 ctctaaccca gagacgtccg tctccggtgt ttcgtccttt ccacaagata tataaagcca
     2641 agaaatcgaa atactttcaa gttacggtaa gcatatgata gtccatttta aaacataatt
     2701 ttaaaactgc aaactaccca agaaattatt actttctacg tcacgtattt tgtactaata
     2761 tctttgtgtt tacagtcaaa ttaattctaa ttatctctct aacagccttg tatcgtatat
     2821 gcaaatatga aggaatcatg ggaaataggc cctcactagt tttttataac ttcgtataaa
     2881 gtatcctata cgaagttatc ctagaccggt ttttaaaaga aaaggggaat aaatgaaagc
     2941 ttgcagatct gcgactctag aggatctgcg actctagagg atcataatca gccataccac
     3001 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     3061 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     3121 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3181 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgcgactcta gaggatcata
     3241 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     3301 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     3361 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3421 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgcgac
     3481 tctagaggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     3541 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     3601 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3661 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     3721 tctggatccc catcaagctg atccggaacc cttcaaaaac aaattacaaa aattcaaaat
     3781 tttgatcata acttcgtata atgtatgcta tacgaagtta tgctcccggg gccaccatgg
     3841 acaagaagta cagcatcggc ctggacatcg gtaccaacag cgtgggctgg gccgtgatca
     3901 ccgacgagta caaggtgccc agcaagaagt tcaaggtgct gggcaacacc gaccgccaca
     3961 gcatcaagaa gaacctgatc ggcgccctgc tgttcgacag cggcgagacc gccgaggcca
     4021 cccgcctgaa gcgcaccgcc cgccgccgct acacccgccg caagaaccgc atctgctacc
     4081 tgcaggagat cttcagcaac gagatggcca aggtggacga cagcttcttc caccgcctgg
     4141 aggagagctt cctggtggag gaggacaaga agcacgagcg ccaccccatc ttcggcaaca
     4201 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg cgcaagaagc
     4261 tggtggacag caccgacaag gccgacctgc gcctgatcta cctggccctg gcccacatga
     4321 tcaagttccg cggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg
     4381 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggag aaccccatca
     4441 acgccagcgg cgtggacgcc aaggccatcc tgagcgcccg cctgagcaag agccgccgcc
     4501 tggagaacct gatcgcccag ctgcccggcg agaagaagaa cggcctgttc ggcaacctga
     4561 tcgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggacg
     4621 ccaagctgca gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga
     4681 tcggcgacca gtacgccgac ctgttcctgg ccgccaagaa cctgagcgac gccatcctgc
     4741 tgagcgacat cctgcgcgtg aacaccgaga tcaccaaggc ccccctgagc gccagcatga
     4801 tcaagcgcta cgacgagcac caccaggacc tgaccctgct gaaggccctg gtgcgccagc
     4861 agctgcccga gaagtacaag gagatcttct tcgaccagag caagaacggc tacgccggct
     4921 acatcgacgg cggcgccagc caggaggagt tctacaagtt catcaagccc atcctggaga
     4981 agatggacgg caccgaggag ctgctggtga agctgaaccg cgaggacctg ctgcgcaagc
     5041 agcgcacctt cgacaacggc agcatccccc accagatcca cctgggcgag ctgcacgcca
     5101 tcctgcgccg ccaggaggac ttctacccct tcctgaagga caaccgcgag aagatcgaga
     5161 agatcctgac cttccgcatc ccctactacg tgggccccct ggcccgcggc aacagccgct
     5221 tcgcctggat gacccgcaag agcgaggaga ccatcacccc ctggaacttc gaggaggtgg
     5281 tggacaaggg cgccagcgcc cagagcttca tcgagcgcat gaccaacttc gacaagaacc
     5341 tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtaca
     5401 acgagctgac caaggtgaag tacgtgaccg agggcatgcg caagcccgcc ttcctgagcg
     5461 gcgagcagaa gaaggccatc gtggacctgc tgttcaagac caaccgcaag gtgaccgtga
     5521 agcagctgaa ggaggactac ttcaagaaga tcgagtgctt cgacagcgtg gagatcagcg
     5581 gcgtggagga ccgcttcaac gccagcctgg gcacctacca cgacctgctg aagatcatca
     5641 aggacaagga cttcctggac aacgaggaga acgaggacat cctggaggac atcgtgctga
     5701 ccctgaccct gttcgaggac cgcgagatga tcgaggagcg cctgaagacc tacgcccacc
     5761 tgttcgacga caaggtgatg aagcagctga agcgccgccg ctacaccggc tggggccgcc
     5821 tgagccgcaa gcttatcaac ggcatccgcg acaagcagag cggcaagacc atcctggact
     5881 tcctgaagag cgacggcttc gccaaccgca acttcatgca gctgatccac gacgacagcc
     5941 tgaccttcaa ggaggacatc cagaaggccc aggtgagcgg ccagggcgac agcctgcacg
     6001 agcacatcgc caacctggcc ggcagccccg ccatcaagaa gggcatcctg cagaccgtga
     6061 aggtggtgga cgagctggtg aaggtgatgg gccgccacaa gcccgagaac atcgtgatcg
     6121 agatggcccg cgagaaccag accacccaga agggccagaa gaacagccgc gagcgcatga
     6181 agcgcatcga ggagggcatc aaggagctgg gcagccagat cctgaaggag caccccgtgg
     6241 agaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaac ggccgcgaca
     6301 tgtacgtgga ccaggagctg gacatcaacc gcctgagcga ctacgacgtg gaccacatcg
     6361 tgccccagag cttcctgaag gacgacagca tcgacaacaa ggtgctgacc cgcagcgaca
     6421 agaaccgcgg caagagcgac aacgtgccca gcgaggaggt ggtgaagaag atgaagaact
     6481 actggcgcca gctgctgaac gccaagctga tcacccagcg caagttcgac aacctgacca
     6541 aggccgagcg cggcggcctg agcgagctgg acaaggccgg cttcatcaag cgccagctgg
     6601 tggagacccg ccagatcacc aagcacgtgg cccagatcct ggacagccgc atgaacacca
     6661 agtacgacga gaacgacaag ctgatccgcg aggtgaaggt gatcaccctg aagagcaagc
     6721 tggtgagcga cttccgcaag gacttccagt tctacaaggt gcgcgagatc aacaactacc
     6781 accacgccca cgacgcctac ctgaacgccg tggtgggcac cgccctgatc aagaagtacc
     6841 ccaagctgga gagcgagttc gtgtacggcg actacaaggt gtacgacgtg cgcaagatga
     6901 tcgccaagag cgagcaggag atcggcaagg ccaccgccaa gtacttcttc tacagcaaca
     6961 tcatgaactt cttcaagacc gagatcaccc tggccaacgg cgagatccgc aagcgccccc
     7021 tgatcgagac caacggcgag accggcgaga tcgtgtggga caagggccgc gacttcgcca
     7081 ccgtgcgcaa ggtgctgagc atgccccagg tgaacatcgt gaagaagacc gaggtgcaga
     7141 ccggcggctt cagcaaggag agcatcctgc ccaagcgcaa cagcgacaag ctgatcgccc
     7201 gcaagaagga ctgggacccc aagaagtacg gcggcttcga cagccccacc gtggcctaca
     7261 gcgtgctggt ggtggccaag gtggagaagg gcaagagcaa gaagctgaag agcgtgaagg
     7321 agctgctggg catcaccatc atggagcgca gcagcttcga gaagaacccc atcgacttcc
     7381 tggaggccaa gggctacaag gaggtgaaga aggacctgat catcaagctg cccaagtaca
     7441 gcctgttcga gctggagaac ggccgcaagc gcatgctggc cagcgccggc gagctgcaga
     7501 agggcaacga gctggccctg cccagcaagt acgtgaactt cctgtacctg gccagccact
     7561 acgagaagct gaagggcagc cccgaggaca acgagcagaa gcagctgttc gtggagcagc
     7621 acaagcacta cctggacgag atcatcgagc agatcagcga gttcagcaag cgcgtgatcc
     7681 tggccgacgc caacctggac aaggtgctga gcgcctacaa caagcaccgc gacaagccca
     7741 tccgcgagca ggccgagaac atcatccacc tgttcaccct gaccaacctg ggcgcccccg
     7801 ccgccttcaa gtacttcgac accaccatcg accgcaagcg ctacaccagc accaaggagg
     7861 tgctggacgc caccctgatc caccagagca tcaccggtct gtacgagacc cgcatcgacc
     7921 tgagccagct gggcggcgac ggcggctccg gacctccaaa gaaaaagaga aaagtatacc
     7981 cctacgacgt gcccgactac gccgaattcg gaagcgggca gtgcaccaac tacgccctgc
     8041 tgaagctggc cggcgacgtg gagagcaacc ccggccccgg atccatggtg agcaagggcg
     8101 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     8161 acaagttcag cgtccgcggc gagggcgagg gcgatgccac caacggcaag ctgaccctga
     8221 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg
     8281 gctacggcgt ggcctgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     8341 agtccgccat gcccgaaggc tacgtccagg agcgcaccat ctctttcaag gacgacggta
     8401 cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     8461 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     8521 tcaacagcca ctacgtctat atcacggccg acaagcagaa gaactgcatc aaggctaact
     8581 tcaagatccg ccacaacgtt gaggacggca gcgtgcagct cgccgaccac taccagcaga
     8641 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agccatcagt
     8701 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     8761 ccgccgccgg gattacacat ggcatggacg agctgtacaa gtgacccggc gcgttaagtc
     8821 gagctcgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     8881 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     8941 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     9001 agcaagtaaa acctctacaa atgtggtact taagagggat cgataaggat ctagcttgtg
     9061 gaaggctact cgaaatgttt gacccaagtt aaacaattta aaggcaatgc taccaaatac
     9121 taattgagtg tatgtaaact tctgacccac tgggaatgtg atgaaagaaa taaaagctga
     9181 aatgaatcat tctctctact attattctga tatttcacat tcttaaaata aagtggtcct
     9241 aggagctagt caggtagtaa aaggtctcaa ctaggcagtg gcagagcagg attcaaattc
     9301 agggctgttg tgatgcctcc gcagactctg agcgccacct ggtggtaatt tgtctgtgcc
     9361 tcttctgacg tggaagaaca gcaactaaca cactaacacg gcatttacta tgggccagcc
     9421 attgttctaa gatcctaact gacctaagac agggaatttt tactaggatt aaatgtcagg
     9481 aattgtgaaa aagtgagttt aaatgtattt ggctaaggtg tatgtaaact tccgacttca
     9541 actgtatagg gatcctctag ctagagtcga cctcgagggg gggcccggta cccagctttt
     9601 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     9661 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9721 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9781 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9841 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9901 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9961 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    10021 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    10081 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    10141 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    10201 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10261 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10321 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10381 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10441 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    10501 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10561 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10621 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10681 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10741 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10801 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10861 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10921 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10981 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    11041 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    11101 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    11161 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    11221 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    11281 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    11341 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    11401 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    11461 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    11521 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11581 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11641 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11701 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    11761 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
    11821 gcgcggcggg agtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
    11881 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
    11941 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
    12001 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
    12061 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
    12121 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
    12181 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
    12241 cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    12301 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    12361 ggtaacgcca
//
