LOCUS       pCAGGS.seqT             5645 bp    DNA     circular SYN 01-Apr-2007
DEFINITION  .
FEATURES             Location/Qualifiers
     source          <1748..>2455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
                     /note=" [Split]"
     CDS             1..1628
                     /note="CAG promoter"
     CDS             join(1631..>1702,<2617..3120)
                     /note="rabbir beta-globin polyA [Split]"
     frag            1703..2616
                     /date="16-1#-2018"
                     /note="1 to 914 of mCherry2-G4-pCAGS"
     frag            1703..1726
                     /date="04-12#-2017"
                     /left_end="0"
                     /note="1703 to 1726 of pCAGGS 1726-RV"
                     /right_end="0"
     CDS             1703..1726
                     /note="pCAGGS-172 6"
     frag            1728..>1747
                     /date="04-12#-2017"
                     /note="592 to 1456 of pmKate2-G (OLD NheI Site####)
                     [Split]"
     CDS             1728..>1747
                     /note="mKate2 [Split]"
     frag            1748..2455
                     /date="04-12#-2017"
                     /left_end="0"
                     /note="553 to 1260 of mCherry2 ORF wos"
                     /right_end="0"
     regulatory      <1748..1751
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987) [Split]"
     CDS             1748..2455
                     /codon_start=1
                     /label="mCherry2"
                     /note="mammalian codon-optimized"
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1893..1911)
                     /label="mCherry-R"
                     /note="mCherry, reverse primer"
     primer_bind     complement(1943..1957)
                     /label="DsRed1-N"
                     /note="DsRed1, reverse primer"
     primer_bind     2358..2378
                     /label="DsRed1-C"
                     /note="DsRed1, forward primer"
     CDS             <2456
     frag            <2456..2591
     CDS             2457..2513
                     /note="GGGS4"
     CDS             <2457..2465
                     /codon_start=3
                     /gene="AcGFP-[GGGS]x3"
                     /note=" [Split]"
     frag            <2457..>2513
                     /note=" [Split]"
     frag            <2457..>2513
                     /note=" [Split]"
     frag            2457..2513
                     /date="14-12#-2016"
                     /left_cutter="BspEI (1330)"
                     /left_end="5CCGG"
                     /note="1331 to 1387 of pAcGFP-G"
                     /right_cutter="BglII (1387)"
                     /right_end="5GATC"
     CDS             2592..2616
                     /note="pCAGS-1745Fw"
     frag            2592..2616
                     /date="04-12#-2017"
                     /left_end="0"
                     /note="1745 to 1769 of pCAGGS 1745-Fw"
                     /right_end="0"
     CDS             3316..3652
                     /note="SV40 ori"
     CDS             complement(4646..5576)
                     /note="AmpR"
BASE COUNT  1182 A  1534 C  1612 G  1317 T  0 OTHER
ORIGIN
        1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA
       61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC
      121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG
      181 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC
      241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG
      301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG
      361 TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA
      421 TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG
      481 CGATGGGGGC GGGGGGGGGG GGGGGGCGCG CGCCAGGCGG GGCGGGGCGG GGCGAGGGGC
      541 GGGGCGGGGC GAGGCGGAGA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT
      601 TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG
      661 CGGGAGTCGC TGCGCGCTGC CTTCGCCCCG TGCCCCGCTC CGCGCCGCCT CGCGCCGCCC
      721 GCCCCGGCTC TGACTGACCG CGTTACTCCC ACAGGTGAGC GGGCGGGACG GCCCTTCTCC
      781 TCCGGGCTGT AATTAGCGCT TGGTTTAATG ACGGCTTGTT TCTTTTCTGT GGCTGCGTGA
      841 AAGCCTTGAG GGGCTCCGGG AGGGCCCTTT GTGCGGGGGG AGCGGCTCGG GGGGTGCGTG
      901 CGTGTGTGTG TGCGTGGGGA GCGCCGCGTG CGGCTCCGCG CTGCCCGGCG GCTGTGAGCG
      961 CTGCGGGCGC GGCGCGGGGC TTTGTGCGCT CCGCAGTGTG CGCGAGGGGA GCGCGGCCGG
     1021 GGGCGGTGCC CCGCGGTGCG GGGGGGGCTG CGAGGGGAAC AAAGGCTGCG TGCGGGGTGT
     1081 GTGCGTGGGG GGGTGAGCAG GGGGTGTGGG CGCGTCGGTC GGGCTGCAAC CCCCCCTGCA
     1141 CCCCCCTCCC CGAGTTGCTG AGCACGGCCC GGCTTCGGGT GCGGGGCTCC GTACGGGGCG
     1201 TGGCGCGGGG CTCGCCGTGC CGGGCGGGGG GTGGCGGCAG GTGGGGGTGC CGGGCGGGGC
     1261 GGGGCCGCCT CGGGCCGGGG AGGGCTCGGG GGAGGGGCGC GGCGGCCCCC GGAGCGCCGG
     1321 CGGCTGTCGA GGCGCGGCGA GCCGCAGCCA TTGCCTTTTA TGGTAATCGT GCGAGAGGGC
     1381 GCAGGGACTT CCTTTGTCCC AAATCTGTGC GGAGCCGAAA TCTGGGAGGC GCCGCCGCAC
     1441 CCCCTCTAGC GGGCGCGGGG CGAAGCGGTG CGGCGCCGGC AGGAAGGAAA TGGGCGGGGA
     1501 GGGCCTTCGT GCGTCGCCGC GCCGCCGTCC CCTTCTCCCT CTCCAGCCTC GGGGCTGTCC
     1561 GCGGGGGGAC GGCTGCCTTC GGGGGGGACG GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT
     1621 GACCGGCGGC TCTAGAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT
     1681 CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGCTA GCCTACCGGT
     1741 CGCCACCATG GTGAGCAAGG GCGAGGAGGA TAACATGGCC ATCATCAAGG AGTTCATGCG
     1801 CTTCAAGGTG CACATGGAGG GCTCCGTGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG
     1861 CGAGGGCCGC CCCTACGAGG GCACCCAGAC CGCCAAGCTG AAGGTGACCA AGGGTGGCCC
     1921 CCTGCCCTTC GCCTGGGACA TCCTGTCCCC TCAGTTCATG TACGGCTCCA AGGCCTACGT
     1981 GAAGCACCCC GCCGACATCC CCGACTACTT GAAGCTGTCC TTCCCCGAGG GCTTCAATTG
     2041 GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGTGACC GTGACCCAGG ACTCCTCCCT
     2101 GCAGGACGGC GAGTTCATCT ACAAGGTGAA GCTGCGCGGC ACCAACTTCC CCTCCGACGG
     2161 CCCCGTAATG CAGTGTCGTA CCATGGGCTG GGAGGCCTCC ACTGAGCGGA TGTACCCCGA
     2221 GGACGGCGCC CTGAAGGGCG AGATCAAGCA GAGGCTGAAG CTGAAGGACG GCGGCCACTA
     2281 CGACGCTGAG GTCAAGACCA CCTACAAGGC CAAGAAGCCC GTGCAGCTGC CCGGCGCCTA
     2341 CAACGTCGAC ATCAAGTTGG ACATCCTTTC CCACAACGAG GACTACACCA TCGTGGAACA
     2401 GTACGAACGC GCCGAGGGCC GCCACTCCAC CGGCGGCATG GACGAGCTGT ACAAGTCCGG
     2461 ACTCACCGGT GGAGGCGGTT CAGGCGGAGG TGGCTCTGGC GGTGGCGGAT CGAGATCTCG
     2521 AGCTCAAGCT TCGAATTCTG CAGTCGACGG TACCGCGGGC CCGGGATCCA CCGGATCTAG
     2581 ATAACTGATC AACTCCTCAG GTGCAGGCTG CCTATCAGAA GGTGGTGGCT GGTGTGGCCA
     2641 ATGCCCTGGC TCACAAATAC CACTGAGATC TTTTTCCCTC TGCCAAAAAT TATGGGGACA
     2701 TCATGAAGCC CCTTGAGCAT CTGACTTCTG GCTAATAAAG GAAATTTATT TTCATTGCAA
     2761 TAGTGTGTTG GAATTTTTTG TGTCTCTCAC TCGGAAGGAC ATATGGGAGG GCAAATCATT
     2821 TAAAACATCA GAATGAGTAT TTGGTTTAGA GTTTGGCAAC ATATGCCATA TGCTGGCTGC
     2881 CATGAACAAA GGTGGCTATA AAGAGGTCAT CAGTATATGA AACAGCCCCC TGCTGTCCAT
     2941 TCCTTATTCC ATAGAAAAGC CTTGACTTGA GGTTAGATTT TTTTTATATT TTGTTTTGTG
     3001 TTATTTTTTT CTTTAACATC CCTAAAATTT TCCTTACATG TTTTACTAGC CAGATTTTTC
     3061 CTCCTCTCCT GACTACTCCC AGTCATAGCT GTCCCTCTTC TCTTATGAAG ATCCCTCGAC
     3121 CTGCAGCCCA AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC
     3181 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA
     3241 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA
     3301 CCTGTCGTGC CAGCGGATCC GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT
     3361 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA
     3421 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG
     3481 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TAACTTGTTT ATTGCAGCTT
     3541 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC
     3601 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCCGCT
     3661 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC
     3721 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA
     3781 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
     3841 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA
     3901 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     3961 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     4021 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     4081 GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     4141 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     4201 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     4261 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     4321 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     4381 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     4441 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT
     4501 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG
     4561 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT
     4621 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC
     4681 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
     4741 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC
     4801 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG
     4861 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG
     4921 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT
     4981 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG
     5041 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT
     5101 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC
     5161 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC
     5221 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA
     5281 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG
     5341 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC
     5401 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG
     5461 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT
     5521 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
     5581 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
     5641 ACCTG
//
