LOCUS       pAAV-CMV                5916 bp    DNA     circular SYN 28-Apr-2014
DEFINITION  .
SOURCE      synthetic DNA construct
COMMENT     Clontech Laboratories Inc. http://www.clontech.com
            Cat. No. 6230
FEATURES             Location/Qualifiers
     source          <1535..>2242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
                     /note=" [Split]"
     repeat_region   237..377
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     Read            237..377
                     /note="AAV2 ITR"
     enhancer        446..749
                     /label="CMV enhancer"
     promoter        750..953
                     /label="CMV promoter"
     intron          988..1480
                     /label="Human beta-globin intron"
     misc_feature    1481..>1486
                     /label="MCS"
                     /note="Multiple Cloning Site"
     CDS             <1487..1489
                     /note="rabbir beta-globin polyA [Split]"
     frag            1487..>1489
                     /date="08-5#-2018"
                     /note="1700 to 2571 of pCAG-hCoGFP"
     frag            1490..1513
                     /date="04-12#-2017"
                     /left_end="0"
                     /note="1703 to 1726 of pCAGGS 1726-RV"
                     /right_end="0"
     frag            1490..>2300
                     /date="16-1#-2018"
                     /note="1 to 914 of mCherry2-G4-pCAGS [Split]"
     frag            1490..2300
                     /date="02-Dec-2020"
                     /note="1703 to 2513 of pCAG-mCherry2-G4"
     frag            1490..>2300
                     /date="16-1#-2018"
                     /note="1 to 914 of mWasabi-G4-pCAGS [Split]"
     frag            1490..1513
                     /date="04-12#-2017"
                     /left_end="0"
                     /note="1703 to 1726 of pCAGGS 1726-RV"
                     /right_end="0"
     frag            1490..2300
                     /date="02-Dec-2020"
                     /note="1703 to 2513 of pCAG-mWasabi-G4"
     frag            1490..>2300
                     /date="16-1#-2018"
                     /note="1 to 914 of mWasabi-G4-pCAGS [Split]"
     frag            1490..2300
                     /date="02-Dec-2020"
                     /note="1703 to 2513 of pCAG-mWasabi-G4"
     frag            1490..1513
                     /date="04-12#-2017"
                     /left_end="0"
                     /note="1703 to 1726 of pCAGGS 1726-RV"
                     /right_end="0"
     CDS             1490..1513
                     /note="pCAGGS-172 6"
     CDS             1515..>1534
                     /note="mKate2 [Split]"
     frag            1515..>1534
                     /date="04-12#-2017"
                     /note="592 to 1456 of pmKate2-G (OLD NheI Site####)
                     [Split]"
     frag            1515..>1534
                     /date="04-12#-2017"
                     /note="592 to 1456 of pmKate2-G (OLD NheI Site####)
                     [Split]"
     CDS             1515..>1534
                     /note="mKate2 [Split]"
     frag            1515..>1534
                     /date="04-12#-2017"
                     /note="592 to 1456 of pmKate2-G (OLD NheI Site####)
                     [Split]"
     CDS             1515..>1534
                     /note="mKate2 [Split]"
     frag            1535..>2242
                     /note="613 to 1386 of pmWasabi-C [Split]"
     frag            1535..2242
                     /date="04-12#-2017"
                     /left_end="0"
                     /note="1 to 708 of mWasabi minORF"
                     /right_end="0"
     frag            1535..2242
                     /date="04-12#-2017"
                     /left_end="0"
                     /note="1 to 708 of mWasabi minORF"
                     /right_end="0"
     frag            1535..>2242
                     /note="613 to 1386 of pmWasabi-C [Split]"
     CDS             1535..2242
                     /note="mWasabi"
     frag            1535..2242
                     /date="04-12#-2017"
                     /left_end="0"
                     /note="553 to 1260 of mCherry2 ORF wos"
                     /right_end="0"
     regulatory      <1535..1538
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987) [Split]"
     primer_bind     complement(1680..1698)
                     /label="mCherry-R"
                     /note="mCherry, reverse primer"
     primer_bind     complement(1730..1744)
                     /label="DsRed1-N"
                     /note="DsRed1, reverse primer"
     CDS             <2243
     frag            2243..>2300
                     /note=" [Split]"
     frag            2243..>2300
                     /note=" [Split]"
     frag            2243..>2300
                     /note=" [Split]"
     CDS             <2243
     CDS             <2243
     CDS             2244..2300
                     /note="GGGS4"
     CDS             <2244..2252
                     /codon_start=3
                     /gene="AcGFP-[GGGS]x3"
                     /note=" [Split]"
     frag            <2244..>2300
                     /note=" [Split]"
     frag            <2244..>2300
                     /note=" [Split]"
     frag            2244..2300
                     /date="14-12#-2016"
                     /left_cutter="BspEI (1330)"
                     /left_end="5CCGG"
                     /note="1331 to 1387 of pAcGFP-G"
                     /right_cutter="BglII (1387)"
                     /right_end="5GATC"
     frag            2244..2300
                     /date="14-12#-2016"
                     /left_cutter="BspEI (1330)"
                     /left_end="5CCGG"
                     /note="1331 to 1387 of pAcGFP-G"
                     /right_cutter="BglII (1387)"
                     /right_end="5GATC"
     CDS             <2244..2252
                     /codon_start=3
                     /gene="AcGFP-[GGGS]x3"
                     /note=" [Split]"
     frag            2244..2300
                     /date="14-12#-2016"
                     /left_cutter="BspEI (1330)"
                     /left_end="5CCGG"
                     /note="1331 to 1387 of pAcGFP-G"
                     /right_cutter="BglII (1387)"
                     /right_end="5GATC"
     frag            <2244..>2300
                     /note=" [Split]"
     frag            <2244..>2300
                     /note=" [Split]"
     CDS             <2244..2252
                     /codon_start=3
                     /gene="AcGFP-[GGGS]x3"
                     /note=" [Split]"
     frag            <2244..>2300
                     /note=" [Split]"
     frag            <2244..>2300
                     /note=" [Split]"
     frag            <2301..2403
                     /date="12-Jun-2019"
                     /note="1703 to 2610 of pCAG-mRuby3-HO-G4"
     frag            <2301..2403
                     /date="25-1#-2018"
                     /note="1 to 869 of hCoGFP-G4-pCAGS [Split]"
     frag            <2301..2378
     frag            2379..2403
                     /date="04-12#-2017"
                     /left_end="0"
                     /note="1745 to 1769 of pCAGGS 1745-Fw"
                     /right_end="0"
     CDS             2379..2403
                     /note="pCAGS-1745Fw"
     polyA_signal    2406..2882
                     /label="hGH polyA signal"
                     /note="human growth hormone polyadenylation signal"
     repeat_region   2895..3035
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     Read            2895..3035
                     /note="AAV2 ITR"
     Read            <3158..3160
                     /note=" [Split]"
     Read            <3158..>3367
                     /note="pmNeonGreenHOmtActA-CMV-F_D03 [Split]"
     Read            complement(<3158..>3367)
                     /note=" [Split]"
     Read            <3158..3262
                     /note=" [Split]"
     Read            3223..3367
                     /note="mt-signal_ActA"
     rep_origin      3274..3729
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication"
     promoter        4011..4115
                     /label="AmpR promoter"
     CDS             4116..4976
                     /label="AmpR"
     rep_origin      5147..5735
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
BASE COUNT  1388 A  1564 C  1508 G  1456 T  0 OTHER
ORIGIN
        1 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC
       61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC
      121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA
      181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GAATTGCCTG
      241 CAGGCAGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG GGCGTCGGGC
      301 GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG TGGCCAACTC
      361 CATCACTAGG GGTTCCTATC GATATCAAGC TTTAATAGTA ATCAATTACG GGGTCATTAG
      421 TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT
      481 GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC
      541 CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG
      601 CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT
      661 GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA
      721 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC
      781 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA
      841 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT
      901 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTGGTTTAG
      961 TGGATATCCT TAAGGGCCCA GCCGGCCCGA ATCCCGGCCG GGAACGGTGC ATTGGAACGC
     1021 GGATTCCCCG TGCCAAGAGT GACGTAAGTA CCGCCTATAG AGTCTATAGG CCCACAAAAA
     1081 ATGCTTTCTT CTTTTAATAT ACTTTTTTGT TTATCTTATT TCTAATACTT TCCCTAATCT
     1141 CTTTCTTTCA GGGCAATAAT GATACAATGT ATCATGCCTC TTTGCACCAT TCTAAAGAAT
     1201 AACAGTGATA ATTTCTGGGT TAAGGCAATA GCAATATTTC TGCATATAAA TATTTCTGCA
     1261 TATAAATTGT AACTGATGTA AGAGGTTTCA TATTGCTAAT AGCAGCTACA ATCCAGCTAC
     1321 CATTCTGCTT TTATTTTATG GTTGGGATAA GGCTGGATTA TTCTGAGTCC AAGCTAGGCC
     1381 CTTTTGCTAA TCATGTTCAT ACCTCTTATC TTCCTCCCAC AGCTCCTGGG CAACGTGCTG
     1441 GTCTGTGTGC TGGCCCATCA CTTTGGCAAA GAATTGGGAT TCGCGAATTG TGCTGTCTCA
     1501 TCATTTTGGC AAAGCTAGCC TACCGGTCGC CACCATGGTG AGCAAGGGCG AGGAGACCAC
     1561 AATGGGCGTA ATCAAGCCCG ACATGAAGAT CAAGCTGAAG ATGGAGGGCA ACGTGAATGG
     1621 CCACGCCTTC GTGATCGAGG GCGAGGGCGA GGGCAAGCCC TACGACGGCA CCAACACCAT
     1681 CAACCTGGAG GTGAAGGAGG GAGCCCCCCT GCCCTTCTCC TACGACATTC TGACCACCGC
     1741 GTTCAGTTAC GGCAACAGGG CCTTCACCAA GTACCCCGAC GACATCCCCA ACTACTTCAA
     1801 GCAGTCCTTC CCCGAGGGCT ACTCTTGGGA GCGCACCATG ACCTTCGAGG ACAAGGGCAT
     1861 CGTGAAGGTG AAGTCCGACA TCTCCATGGA GGAGGACTCC TTCATCTACG AGATACACCT
     1921 CAAGGGCGAG AACTTCCCCC CCAACGGCCC CGTGATGCAG AAGGAGACCA CCGGCTGGGA
     1981 CGCCTCCACC GAGAGGATGT ACGTGCGCGA CGGCGTGCTG AAGGGCGACG TCAAGATGAA
     2041 GCTGCTGCTG GAGGGCGGCG GCCACCACCG CGTTGACTTC AAGACCATCT ACAGGGCCAA
     2101 GAAGGCGGTG AAGCTGCCCG ACTATCACTT TGTGGACCAC CGCATCGAGA TCCTGAACCA
     2161 CGACAAGGAC TACAACAAGG TGACCGTTTA CGAGATCGCC GTGGCCCGCA ACTCCACCGA
     2221 CGGCATGGAC GAGCTGTACA AGTCCGGACT CACCGGTGGA GGCGGTTCAG GCGGAGGTGG
     2281 CTCTGGCGGT GGCGGATCGA GATCTCGAGC TCAAGCTTCG AATTCTGCAG TCGACGGTAC
     2341 CGCGGGCCCG GGATCCACCG GATCTAGATA ACTGATCAAC TCCTCAGGTG CAGGCTGCCT
     2401 ATCACGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA
     2461 CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG
     2521 TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA
     2581 AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT
     2641 CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG
     2701 AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA
     2761 GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC
     2821 CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC
     2881 TTATCGATAG ATCTAGGAAC CCCTAGTGAT GGAGTTGGCC ACTCCCTCTC TGCGCGCTCG
     2941 CTCGCTCACT GAGGCCGGGC GACCAAAGGT CGCCCGACGC CCGGGCTTTG CCCGGGCGGC
     3001 CTCAGTGAGC GAGCGAGCGC GCAGCTGCCT GCAGGCAGCT TGGCACTGGC CGTCGTTTTA
     3061 CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC
     3121 CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG
     3181 CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT
     3241 ATTTCACACC GCATACGTCA AAGCAACCAT AGTACGCGCC CTGTAGCGGC GCATTAAGCG
     3301 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG
     3361 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC
     3421 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA
     3481 AACTTGATTT GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC
     3541 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC
     3601 TCAACCCTAT CTCGGGCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT
     3661 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGT
     3721 TTACAATTTT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC
     3781 CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG
     3841 CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT
     3901 CACCGAAACG CGCGAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA
     3961 TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC
     4021 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT
     4081 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG
     4141 CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG
     4201 TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC
     4261 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA
     4321 CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC
     4381 TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA
     4441 AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG
     4501 ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT
     4561 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG
     4621 AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC
     4681 GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA
     4741 TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA
     4801 TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC
     4861 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG
     4921 ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT
     4981 CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA
     5041 GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT
     5101 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT
     5161 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT
     5221 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA
     5281 TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG
     5341 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA
     5401 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG
     5461 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA
     5521 GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA
     5581 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA
     5641 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT
     5701 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC
     5761 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT
     5821 CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA
     5881 CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAG
//
