LOCUS       Exported                5262 bp    DNA     circular SYN 06-Aug-2018
DEFINITION  An AAV genome with tet-inducible expression of the fluorescent 
            protein mTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-TRE-mTurquoise2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Chan KY, Jang MJ, Yoo BB, Greenbaum A, Ravi N, Wu WL, 
            Sanchez-Guardado L, Lois C, Mazmanian SK, Deverman BE, Gradinaru V
  TITLE     Engineered AAVs for efficient noninvasive gene delivery to the 
            central and peripheral nervous systems.
  JOURNAL   Nat Neurosci. 2017 Aug;20(8):1172-1179. doi: 10.1038/nn.4593. Epub 
            2017 Jun 26.
   PUBMED   28671695
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1366..>5262,1..>551)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
                     /note=" [Split]"
     repeat_region   1..130
                     /label="AAV2 ITR (alternate)"
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             165..442
                     /note="8 x tetO (tet operator)"
     protein_bind    165..183
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    202..220
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    239..257
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    276..294
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    313..331
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    350..368
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    387..405
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    424..442
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     464..488
                     /label="LNCX"
                     /note="Human CMV promoter, forward primer"
     frag            555..1365
                     /date="31-Jan-2019"
                     /note="1703 to 2513 of pCAG-mWasabi-G4"
     frag            555..>1365
                     /date="16-1#-2018"
                     /note="1 to 914 of mWasabi-G4-pCAGS [Split]"
     frag            555..578
                     /date="04-12#-2017"
                     /left_end="0"
                     /note="1703 to 1726 of pCAGGS 1726-RV"
                     /right_end="0"
     CDS             555..578
                     /note="pCAGGS-172 6"
     CDS             580..>599
                     /note="mKate2 [Split]"
     frag            580..>599
                     /date="04-12#-2017"
                     /note="592 to 1456 of pmKate2-G (OLD NheI Site####)
                     [Split]"
     frag            600..1307
                     /date="04-12#-2017"
                     /left_end="0"
                     /note="1 to 708 of mWasabi minORF"
                     /right_end="0"
     frag            600..>1307
                     /note="613 to 1386 of pmWasabi-C [Split]"
     CDS             600..1307
                     /note="mWasabi"
     frag            1308..>1365
                     /note=" [Split]"
     CDS             <1308
     frag            1309..1365
                     /date="14-12#-2016"
                     /left_cutter="BspEI (1330)"
                     /left_end="5CCGG"
                     /note="1331 to 1387 of pAcGFP-G"
                     /right_cutter="BglII (1387)"
                     /right_end="5GATC"
     frag            <1309..>1365
                     /note=" [Split]"
     frag            <1309..>1365
                     /note=" [Split]"
     CDS             <1309..1317
                     /codon_start=3
                     /gene="AcGFP-[GGGS]x3"
                     /note=" [Split]"
     CDS             1309..1365
                     /note="GGGS4"
     Read            1366..1392
                     /note="CAG-1726F"
     misc_feature    1389..1977
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     Read            complement(1393..1417)
                     /note="CAG1745Rv"
     primer_bind     complement(1442..1462)
                     /label="WPRE-R"
                     /note="WPRE, reverse primer"
     CDS             complement(1860..1871)
                     /codon_start=1
                     /label="Factor Xa site"
                     /product="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     polyA_signal    2009..2485
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2208..2227)
                     /label="hGH-PA-R"
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2525..2665
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2525..2654
                     /label="AAV2 ITR"
     rep_origin      2740..3195
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2827..2846)
                     /label="F1ori-R"
                     /note="F1 origin, reverse primer"
     primer_bind     3037..3058
                     /label="F1ori-F"
                     /note="F1 origin, forward primer"
     primer_bind     complement(3212..3231)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3331..3353
                     /label="pGEX 3'"
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3391..3409)
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3477..3581
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             3582..4442
                     /codon_start=1
                     /gene="bla"
                     /label="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3800..3819)
                     /label="Amp-R"
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4613..5201
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5102..5121
                     /label="pBR322ori-F"
                     /note="pBR322 origin, forward primer"
BASE COUNT  1169 A  1440 C  1371 G  1282 T  0 OTHER
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCGGCCGCAC GCGTATCAAG TGCCACCTGA CGTCTCCCTA TCAGTGATAG
      181 AGAAGTCGAC ACGTCTCGAG CTCCCTATCA GTGATAGAGA AGGTACGTCT AGAACGTCTC
      241 CCTATCAGTG ATAGAGAAGT CGACACGTCT CGAGCTCCCT ATCAGTGATA GAGAAGGTAC
      301 GTCTAGAACG TCTCCCTATC AGTGATAGAG AAGTCGACAC GTCTCGAGCT CCCTATCAGT
      361 GATAGAGAAG GTACGTCTAG AACGTCTCCC TATCAGTGAT AGAGAAGTCG ACACGTCTCG
      421 AGCTCCCTAT CAGTGATAGA GAAGCTACCC CCTATATAAG CAGAGCTCGT TTAGTGAACC
      481 GTCAGATCGC CTGGAGACGC CATCCACGCT GTTTTGACCT CCATAGAAGA CACCGGGACC
      541 GATCCAGCCT GATTGTGCTG TCTCATCATT TTGGCAAAGC TAGCCTACCG GTCGCCACCA
      601 TGGTGAGCAA GGGCGAGGAG ACCACAATGG GCGTAATCAA GCCCGACATG AAGATCAAGC
      661 TGAAGATGGA GGGCAACGTG AATGGCCACG CCTTCGTGAT CGAGGGCGAG GGCGAGGGCA
      721 AGCCCTACGA CGGCACCAAC ACCATCAACC TGGAGGTGAA GGAGGGAGCC CCCCTGCCCT
      781 TCTCCTACGA CATTCTGACC ACCGCGTTCA GTTACGGCAA CAGGGCCTTC ACCAAGTACC
      841 CCGACGACAT CCCCAACTAC TTCAAGCAGT CCTTCCCCGA GGGCTACTCT TGGGAGCGCA
      901 CCATGACCTT CGAGGACAAG GGCATCGTGA AGGTGAAGTC CGACATCTCC ATGGAGGAGG
      961 ACTCCTTCAT CTACGAGATA CACCTCAAGG GCGAGAACTT CCCCCCCAAC GGCCCCGTGA
     1021 TGCAGAAGGA GACCACCGGC TGGGACGCCT CCACCGAGAG GATGTACGTG CGCGACGGCG
     1081 TGCTGAAGGG CGACGTCAAG ATGAAGCTGC TGCTGGAGGG CGGCGGCCAC CACCGCGTTG
     1141 ACTTCAAGAC CATCTACAGG GCCAAGAAGG CGGTGAAGCT GCCCGACTAT CACTTTGTGG
     1201 ACCACCGCAT CGAGATCCTG AACCACGACA AGGACTACAA CAAGGTGACC GTTTACGAGA
     1261 TCGCCGTGGC CCGCAACTCC ACCGACGGCA TGGACGAGCT GTACAAGTCC GGACTCACCG
     1321 GTGGAGGCGG TTCAGGCGGA GGTGGCTCTG GCGGTGGCGG ATCGAAATTC GATATCAAGC
     1381 TTATCGATAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT
     1441 ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG
     1501 CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG
     1561 AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA
     1621 CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC
     1681 CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG
     1741 CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT
     1801 GGCTGCTCGC CTATGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT
     1861 CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC
     1921 CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCATC
     1981 GATACCGAGC GCTGCTCGAG AGATCTACGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC
     2041 TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG
     2101 TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT
     2161 ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC
     2221 AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC
     2281 GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG
     2341 CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA
     2401 CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG
     2461 AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTGTAGGTA ACCACGTGCG GACCGAGCGG
     2521 CCGCAGGAAC CCCTAGTGAT GGAGTTGGCC ACTCCCTCTC TGCGCGCTCG CTCGCTCACT
     2581 GAGGCCGGGC GACCAAAGGT CGCCCGACGC CCGGGCTTTG CCCGGGCGGC CTCAGTGAGC
     2641 GAGCGAGCGC GCAGCTGCCT GCAGGGGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG
     2701 TGCGGTATTT CACACCGCAT ACGTCAAAGC AACCATAGTA CGCGCCCTGT AGCGGCGCAT
     2761 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCTTAG
     2821 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC
     2881 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC
     2941 CCAAAAAACT TGATTTGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT
     3001 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA
     3061 CAACACTCAA CTCTATCTCG GGCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG
     3121 TCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT
     3181 TAACGTTTAC AATTTTATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA
     3241 GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG
     3301 CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC
     3361 CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA
     3421 ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG
     3481 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT
     3541 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC
     3601 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA
     3661 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC
     3721 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA
     3781 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG
     3841 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA
     3901 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA
     3961 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA
     4021 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC
     4081 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA
     4141 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG
     4201 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT
     4261 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC
     4321 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA
     4381 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT
     4441 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT
     4501 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG
     4561 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC
     4621 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG
     4681 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG
     4741 CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT
     4801 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG
     4861 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC
     4921 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG
     4981 AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG
     5041 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG
     5101 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC
     5161 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT
     5221 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GT
//
