LOCUS       pAH36                   7544 bp    DNA     circular UNA 17-MAR-2020
DEFINITION  pSCrhaB2 with rhaI1/rhaI1 and stem loop, like pKRJ38.
ACCESSION   urn.local...5l-bovb8c9
VERSION     urn.local...5l-bovb8c9
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1..19
     primer_bind     20..39
     misc_feature    order(7529..>7537,<20..30)
                     /note="Geneious type: primer"
     misc_feature    complement(23..43)
                     /note="Geneious type: primer"
     RBS             31..34
     misc_structure  43..92
     misc_feature    43..62
     misc_feature    complement(165..184)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(194..218)
                     /note="Geneious type: primer"
     CDS             complement(1610..3091)
     rep_origin      3422..3433
     rep_origin      3620..4279
     misc_feature    4729..4756
                     /note="Geneious type: primer"
     misc_feature    4732..4751
                     /note="Geneious type: primer"
     CDS             complement(4892..5128)
     CDS             complement(5517..6536)
     CDS             complement(6439..7272)
     misc_feature    7399
                     /note="Geneious type: promoter uukaryotic"
     misc_feature    complement(7455..7471)
                     /note="Geneious type: binding site"
     misc_feature    7476..>7487
                     /note="Geneious type: primer"
     misc_feature    7487^7488
                     /note="Geneious type: origin"
     misc_feature    7488..7500
                     /note="Geneious type: Editing History Insertion"
     promoter        7501..7536
     misc_feature    <7501..7504
                     /note="Geneious type: binding site"
     misc_feature    complement(7517..7537)
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     7525..7544
     misc_feature    7536
                     /note="Geneious type: Start"
     misc_feature    7538..7544
                     /note="Geneious type: Editing History Insertion"
     misc_feature    complement(7539..>7544)
ORIGIN      
        1 cacaacggtt tccctctagt gaaattcagc aggatcacat atgccatggt acccggggat
       61 cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga gagaagattt
      121 tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg
      181 gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta
      241 gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata
      301 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      361 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc
      421 ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc
      481 atcctgacgg atggcctttt tgcgtttcta caaactcttc cactcactac agcagagcca
      541 tgtagacaac atcccctccc cctttccacc gcgtcagacg cccgtagcag cccgctacgg
      601 gctttttcat gccctgccct agcgtccaag cctcacggcc gcgctcggcc tctctggcgg
      661 ccttctggcg ctgaggtctg cctcgtgaag aaggtgttgc tgactcatac caggcctgaa
      721 tcgccccatc atccagccag aaagtgaggg agccacggtt gatgagagct ttgttgtagg
      781 tggaccagtt ggtgattttg aacttttgct ttgccacgga acggtctgcg ttgtcgggaa
      841 gatgcgtgat ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccgccgtc
      901 ccgtcaagtc agcgtaatgc tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa
      961 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     1021 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     1081 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     1141 tttcccctcg tcaaaaataa ggttatcaag cgagaaatca ccatgagtga cgactgaatc
     1201 cggtgagaat ggcaaaagct aaaaaggccg taatatccag ctgaacggtc tggttatagg
     1261 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     1321 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     1381 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     1441 ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat
     1501 caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt
     1561 cgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     1621 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcgcccgcgt tcctgctggc
     1681 gctgggcctg tttctggcgc tggacttccc gctgttccgt cagcagcttt tcgcccacgg
     1741 ccttgatgat cgcggcggcc ttggcctgca tatcccgatt caacggcccc agggcgtcca
     1801 gaacgggctt caggcgctcc cgaaggtctc gggccgtctc ttgggcttga tcggccttct
     1861 tgcgcatctc acgcgctcct gcggcggcct gtagggcagg ctcatacccc tgccgaaccg
     1921 cttttgtcag ccggtcggcc acggcttccg gcgtctcaac gcgctttgag attcccagct
     1981 tttcggccaa tccctgcggt gcataggcgc gtggctcgac cgcttgcggg ctgatggtga
     2041 cgtggcccac tggtggccgc tccagggcct cgtagaacgc ctgaatgcgc gtgtgacgtg
     2101 ccttgctgcc ctcgatgccc cgttgcagcc ctagatcggc cacagcggcc gcaaacgtgg
     2161 tctggtcgcg ggtcatctgc gctttgttgc cgatgaactc cttggccgac agcctgccgt
     2221 cctgcgtcag cggcaccacg aacgcggtca tgtgcgggct ggtttcgtca cggtggatgc
     2281 tggccgtcac gatgcgatcc gccccgtact tgtccgccag ccacttgtgc gccttctcga
     2341 agaacgccgc ctgctgttct tggctggccg acttccacca ttccgggctg gccgtcatga
     2401 cgtactcgac cgccaacaca gcgtccttgc gccgcttctc tggcagcaac tcgcgcagtc
     2461 ggcccatcgc ttcatcggtg ctgctggccg cccagtgctc gttctctggc gtcctgctgg
     2521 cgtcagcgtt gggcgtctcg cgctcgcggt aggcgtgctt gagactggcc gccacgttgc
     2581 ccattttcgc cagcttcttg catcgcatga tcgcgtatgc cgccatgcct gcccctccct
     2641 tttggtgtcc aaccggctcg acgggggcag cgcaaggcgg tgcctccggc gggccactca
     2701 atgcttgagt atactcacta gactttgctt cgcaaagtcg tgaccgccta cggcggctgc
     2761 ggcgccctac gggcttgctc tccgggcttc gccctgcgcg gtcgctgcgc tcccttgcca
     2821 gcccgtggat atgtggacga tggccgcgag cggccaccgg ctggctcgct tcgctcggcc
     2881 cgtggacaac cctgctggac aagctgatgg acaggctgcg cctgcccacg agcttgacca
     2941 cagggattgc ccaccggcta cccagccttc gaccacatac ccaccggctc caactgcgcg
     3001 gcctgcggcc ttgccccatc aattttttta attttctctg gggaaaagcc tccggcctgc
     3061 ggcctgcgcg cttcgcttgc cggttggaca ccaagtggaa ggcgggtcaa ggctcgcgca
     3121 gcgaccgcgc agcggcttgg ccttgacgcg cctggaacga cccaagccta tgcgagtggg
     3181 ggcagtcgaa ggcgaagccc gcccgcctgc cccccgagcc tcacggcggc gagtgcgggg
     3241 gttccaaggg ggcagcgcca ccttgggcaa ggccgaaggc cgcgcagtcg atcaacaagc
     3301 cccggagggg ccactttttg ccggaggggg agccgcgccg aaggcgtggg ggaaccccgc
     3361 aggggtgccc ttctttgggc accaaagaac tagatatagg gcgaaatgcg aaagacttaa
     3421 aaatcaacaa cttaaaaaag gggggtacgc aacagctcat tgcggcaccc cccgcaatag
     3481 ctcattgcgt aggttaaaga aaatctgtaa ttgactgcca cttttacgca acgcataatt
     3541 gttgtcgcgc tgccgaaaag ttgcagctga ttgcgcatgg tgccgcaacc gtgcggcacc
     3601 ctaccgcatg gagataagca tggccacgca gtccagagaa atcggcattc aagccaagaa
     3661 caagcccggt cactgggtgc aaacggaacg caaagcgcat gaggcgtggg ccgggcttat
     3721 tgcgaggaaa cccacggcgg caatgctgct gcatcacctc gtggcgcaga tgggccacca
     3781 gaacgccgtg gtggtcagcc agaagacact ttccaagctc atcggacgtt ctttgcggac
     3841 ggtccaatac gcagtcaagg acttggtggc cgagcgctgg atctccgtcg tgaagctcaa
     3901 cggccccggc accgtgtcgg cctacgtggt caatgaccgc gtggcgtggg gccagccccg
     3961 cgaccagttg cgcctgtcgg tgttcagtgc cgccgtggtg gttgatcacg acgaccagga
     4021 cgaatcgctg ttggggcatg gcgacctgcg ccgcatcccg accctgtatc cgggcgagca
     4081 gcaactaccg accggccccg gcgaggagcc gcccagccag cccggcattc cgggcatgga
     4141 accagacctg ccagccttga ccgaaacgga ggaatgggaa cggcgcgggc agcagcgcct
     4201 gccgatgccc gatgagccgt gttttctgga cgatggcgag ccgttggagc cgccgacacg
     4261 ggtcacgctg ccgcgccggt agcacttggg ttgcgcagca acccgtaagt gcgctgttcc
     4321 agactatcgg ctgtagccgc ctcgccgccc tataccttgt ctgcctcccc gcgttgcgtc
     4381 gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg
     4441 attcaccgtt tttatcaggc tctgggaggc agaataaatg atcatatcgt caattattac
     4501 ctccacgggg agagcctgag caaactggcc tcaggcattt gagaagcaca cggtcacact
     4561 gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc
     4621 ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta
     4681 ttatcactta ttcaggcgta gcaccaggcg tttaagggca ccaataactc tacggggtct
     4741 gacgctcagt ggaacgaaat cgatgataag ctgtcaaaca tgagcagatc ctctacgccg
     4801 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     4861 acatcaccga tgggagatcc taagatatcg cttaggccac acgttcaagt gcagccacag
     4921 gataaatttg cactgagcct gggtgggatt cggactcgac cgcatagcct tcaggagtga
     4981 gttttgtgca ataccaaccg acgacttgac cctgccaagc ggcaccagat ttcttgcgta
     5041 cgcgatcccc taagccaaag gtggcactca ggggaagcgc aaactgccct gcaacgggag
     5101 cgttggcttc atcgctactt tgacccatgt cgaatccttc ttgtgaatct attatggcga
     5161 caagcaaatc gagctctgac tgcctacccc acaacaacta ctagaaagca ccagcacaac
     5221 ggctgcctaa ctttgtttta gggcgactac cctgctgcgt aacatcgttg ctgttccata
     5281 acatcaaaca tcgacccacg gcgtaacgcg cttagctagc ttggatgccc gaggctagac
     5341 tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
     5401 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta
     5461 cgaaccgaac aggcttatgt caactgggtt cgtgcgagct catcgatatg catttaatct
     5521 ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc cggtttcccg ggtaaacacc
     5581 accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact gattaacagg
     5641 cggctatgct ggagaagata ttgcgcatga cacactctga cctgtcgcag atattgattg
     5701 atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact gcacgatgcc
     5761 tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag ccgggtaatc
     5821 agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt gtaacgatgg
     5881 cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt agcaaacggc
     5941 acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg cgccatcccc
     6001 atgctaccta agcgccagtg tggttgccct gcgctggcgt taaatcccgg aatcgccccc
     6061 tgccagtcaa gattcagctt cagacgctcc gggcaataaa taatattctg caaaaccaga
     6121 tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tgtaaaagag atcgccacgg
     6181 gtaatgcgat aagggcgatc gttgagtaca tgcaggccat taccgcgcca gacaatcacc
     6241 agctcacaaa aatcatgtgt atgttcagca aagacatctt gcggataacg gtcagccaca
     6301 gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa ctgatgcgcc
     6361 accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg tacaaatacg
     6421 ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat cacgcggtga
     6481 ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga atccacagcg
     6541 ataggcgatg tcagtaacgc tggcctcgct gtggcgtagc agatgtcggg ctttcatcag
     6601 tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa gctgccgatg
     6661 tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca cctcatcggc
     6721 aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt tttccaggtt
     6781 ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga tctcgcgact
     6841 ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa ccagctgtcg
     6901 cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc catccagctc
     6961 ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg agcgatacag
     7021 cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat gatcgcgtac
     7081 gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat gaatacccgt
     7141 gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa aatccgcctg
     7201 cgggagccgg ggttctatcg ccacggacgc gttaccagac ggaaaaaaat ccacactatg
     7261 taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa tcacgaggtc
     7321 aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt tttcaagata
     7381 cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaattcag caaattgtga
     7441 acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctggc aaccagggaa
     7501 aggtcgcgaa ttcaggcgct ttttagactg gtcgtaaggg agac
//
