LOCUS       pAH33                   5487 bp    DNA     circular UNA 17-MAR-2020
DEFINITION  pSC201 with rhaI1/rhaI1 and stem loop, like pKRJ38.
ACCESSION   urn.local...37-botql5f
VERSION     urn.local...37-botql5f
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1..19
     primer_bind     20..39
     RBS             31..34
     misc_feature    41..43
                     /note="Geneious type: Start"
     misc_feature    188..379
     misc_feature    complement(232..257)
                     /note="Geneious type: primer"
     misc_feature    360..379
     misc_feature    448..467
     misc_feature    complement(524..549)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(922..1470)
                     /note="Geneious type: insertion"
     misc_feature    complement(2649..2668)
                     /note="Geneious type: primer"
     misc_feature    2675..2694
                     /note="Geneious type: primer"
     CDS             complement(2838..3071)
     CDS             complement(3460..4479)
     CDS             complement(4382..5215)
     primer_bind     5155..5172
     promoter        complement(5240..5275)
     misc_feature    5342
                     /note="Geneious type: promoter uukaryotic"
     misc_feature    complement(5398..5414)
                     /note="Geneious type: binding site"
     misc_feature    5419..>5430
                     /note="Geneious type: primer"
     misc_feature    5430^5431
                     /note="Geneious type: origin"
     misc_feature    5431..5443
                     /note="Geneious type: binding site"
     promoter        5444..5479
     misc_feature    complement(5460..5480)
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     5468..5487
     misc_feature    5480
                     /note="Geneious type: Start"
     misc_feature    5481..5487
                     /note="Geneious type: Editing History Insertion"
     misc_feature    complement(5482..>5487)
ORIGIN      
        1 cacaacggtt tccctctagt gaaattcagc aggatcacat atggatccca agcttcttct
       61 agaggtaccg catgcgatat cgagctctcc cgggaattcg atcgctagtt tgttttgact
      121 ccatccatta gggcttctaa aacgccttct aaggccatgt cagccgttaa gtgttcctgt
      181 gtcactgaaa attgctttga gaggctctaa gggcttctca gtgcgttaca tccctggctt
      241 gttgtccaca accgttaaac cttaaaagct ttaaaagcct tatatattct tttttttctt
      301 ataaaactta aaaccttaga ggctatttaa gttgctgatt tatattaatt ttattgttca
      361 aacatgagag cttagtacgt gaaacatgag agcttagtac gttagccatg agagcttagt
      421 acgttagcca tgagggttta gttcgttaaa catgagagct tagtacgtta aacatgagag
      481 cttagtacgt gaaacatgag agcttagtac gtactatcaa caggttgaac tgctggatcc
      541 tttttgtccg gtgttgggtt gaaggtgaag ccggtcgggg ccgcagcggg ggccggcttt
      601 tcagccttgc ccccctgctt cggccgccgt ggctccggcg tcttgggtgc cggcgcgggt
      661 tccgcagcct tggcctgcgg tgcgggcaca tcggcgggct tggccttgat gtgccgcctg
      721 gcgtgcgagc ggaacgtctc gtaggagaac ttgaccttcc ccgtttcccg catgtgctcc
      781 caaatggtga cgagcgcata gccggacgct aacgccgcct cgacatccgc cctcaccgcc
      841 aggaacgcaa ccgcagcctc atcacgccgg cgcttcttgg ccgcgcggga ttcaacccac
      901 tcggccagct cgtcggtgta gctctttggc atcgtctctc gcctgtcccc tcagttcagt
      961 aatttcctgc atttgcctgt ttccagtcgg tagatattcc acaaaacagc agggaagcag
     1021 cgcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca
     1081 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt
     1141 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc
     1201 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc
     1261 tggccggcta ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca
     1321 accaggaagg gcagcccacc tatcaaggtg tactgccttc cagacgaacg aagagcgatt
     1381 gaggaaaagg cggcggcggc cggcatgagc ctgtcggcct acctgctggc cgtcggccag
     1441 ggctacaaaa tcacgggcgt cgtggactat gagcacgtcc gcgagctggc ccgcatcaat
     1501 ggcgacctgg gccgcctggg cggcctgctg aaactctggc tcaccgacga cccgcgcacg
     1561 gcgcggttcg gtgatgccac gatcctcgcc ctgctggcga agatcgaaga gaagcaggac
     1621 gagcttggca aggtcatgat gggcgtggtc cgcccgaggg cagagccatg acttttttag
     1681 ccgctaaaac ggccgggggg tgcgcgtgat tgccaagcac gtccccatgc gctccatcaa
     1741 gaagagcgac ttcgcggagc tggtgaagta catcaccgac gagcaaggca agaccgagcg
     1801 cctgggtcac gtgcgcgtca cgaactgcga ggcaaacacc ctgcccgctg tcatggccga
     1861 ggtgatggcg acccagcacg gcaacacccg ttccgaggcc gacaagacct atcacctgct
     1921 ggttagcttc cgcgcgggag agaagcccga cgcggagacg ttgcgcgcga ttgaggaccg
     1981 catctgcgct gggcttggct tcgccgagca tcagcgcgtc agtgccgtgc atcacgacac
     2041 cgacaacctg cacatccata tcgccatcaa caagattcac ccgacccgaa acaccatcca
     2101 tgagccgtat cgggcctacc gcgccctcgc tgacctctgc gcgacgctcg aacgggacta
     2161 cgggcttgag cgtgacaatc acgaaacgcg gcagcgcgtt tccgagaacc gcgcgaacga
     2221 catggagcgg cacgcgggcg tggaaagcct ggtcggctgg atcctctacg ccggacgcat
     2281 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     2341 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
     2401 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgctgc ctcgcgcgtt
     2461 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     2521 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     2581 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     2641 tgcggcatca gagcagattg tactgagact cgagacgggg tctgacgctc agtggaacga
     2701 aatcgatgat aagctgtcaa acatgagcag atcctctacg ccggacgcat cgtggccggc
     2761 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatgggaga
     2821 tcctaagata tcgcttaggc cacacgttca agtgcagcca caggataaat ttgcactgag
     2881 cctgggtggg attcggactc gaccgcatag ccttcaggag tgagttttgt gcaataccaa
     2941 ccgacgactt gaccctgcca agcggcacca gatttcttgc gtacgcgatc ccctaagcca
     3001 aaggtggcac tcaggggaag cgcaaactgc cctgcaacgg gagcgttggc ttcatcgcta
     3061 ctttgaccca tgtcgaatcc ttcttgtgaa tctattatgg cgacaagcaa atcgagctct
     3121 gactgcctac cccacaacaa ctactagaaa gcaccagcac aacggctgcc taactttgtt
     3181 ttagggcgac taccctgctg cgtaacatcg ttgctgttcc ataacatcaa acatcgaccc
     3241 acggcgtaac gcgcttagct agcttggatg cccgaggcta gactgtacaa aaaaacagtc
     3301 ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
     3361 ggaccagttg cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta
     3421 tgtcaactgg gttcgtgcga gctcatcgat atgcatttaa tctttctgcg aattgagatg
     3481 acgccactgg ctgggcgtca tcccggtttc ccgggtaaac accaccgaaa aatagttact
     3541 atcttcaaag ccacattcgg tcgaaatatc actgattaac aggcggctat gctggagaag
     3601 atattgcgca tgacacactc tgacctgtcg cagatattga ttgatggtca ttccagtctg
     3661 ctggcgaaat tgctgacgca aaacgcgctc actgcacgat gcctcatcac aaaatttatc
     3721 cagcgcaaag ggacttttca ggctagccgc cagccgggta atcagcttat ccagcaacgt
     3781 ttcgctggat gttggcggca acgaatcact ggtgtaacga tggcgattca gcaacatcac
     3841 caactgcccg aacagcaact cagccatttc gttagcaaac ggcacatgct gactactttc
     3901 atgctcaagc tgaccgataa cctgccgcgc ctgcgccatc cccatgctac ctaagcgcca
     3961 gtgtggttgc cctgcgctgg cgttaaatcc cggaatcgcc ccctgccagt caagattcag
     4021 cttcagacgc tccgggcaat aaataatatt ctgcaaaacc agatcgttaa cggaagcgta
     4081 ggagtgttta tcgtcagcat gaatgtaaaa gagatcgcca cgggtaatgc gataagggcg
     4141 atcgttgagt acatgcaggc cattaccgcg ccagacaatc accagctcac aaaaatcatg
     4201 tgtatgttca gcaaagacat cttgcggata acggtcagcc acagcgactg cctgctggtc
     4261 gctggcaaaa aaatcatctt tgagaagttt taactgatgc gccaccgtgg ctacctcggc
     4321 cagagaacga agttgattat tcgcaatatg gcgtacaaat acgttgagaa gattcgcgtt
     4381 attgcagaaa gccatcccgt ccctggcgaa tatcacgcgg tgaccagtta aactctcggc
     4441 gaaaaagcgt cgaaaagtgg ttactgtcgc tgaatccaca gcgataggcg atgtcagtaa
     4501 cgctggcctc gctgtggcgt agcagatgtc gggctttcat cagtcgcagg cggttcaggt
     4561 atcgctgagg cgtcagtccc gtttgctgct taagctgccg atgtagcgta cgcagtgaaa
     4621 gagaaaattg atccgccacg gcatcccaat tcacctcatc ggcaaaatgg tcctccagcc
     4681 aggccagaag caagttgaga cgtgatgcgc tgttttccag gttctcctgc aaactgcttt
     4741 tacgcagcaa gagcagtaat tgcataaaca agatctcgcg actggcggtc gagggtaaat
     4801 cattttcccc ttcctgctgt tccatctgtg caaccagctg tcgcacctgc tgcaatacgc
     4861 tgtggttaac gcgccagtga gacggatact gcccatccag ctcttgtggc agcaactgat
     4921 tcagcccggc gagaaactga aatcgatccg gcgagcgata cagcacattg gtcagacaca
     4981 gattatcggt atgttcatac agatgccgat catgatcgcg tacgaaacag accgtgccac
     5041 cggtgatggt atagggctgc ccattaaaca catgaatacc cgtgccatgt tcgacaatca
     5101 caatttcatg aaaatcatga tgatgttcag gaaaatccgc ctgcgggagc cggggttcta
     5161 tcgccacgga cgcgttacca gacggaaaaa aatccacact atgtaatacg gtcatactgg
     5221 cctcctgatg tcgtcaacac ggcgaaatag taatcacgag gtcaggttct taccttaaat
     5281 tttcgacgga aaaccacgta aaaaacgtcg atttttcaag atacagcgtg aattttcagg
     5341 aaatgcggtg agcatcacat caccacaatt cagcaaattg tgaacatcat cacgttcatc
     5401 tttccctggt tgccaatggc ccattttcct ggcaaccagg gaaaggtcgc gaattcaggc
     5461 gctttttaga ctggtcgtaa gggagac
//
