LOCUS       Exported                7267 bp ds-DNA     circular SYN 21-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    AAV-hRO-fCX3CL1.ape
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2020 from SnapGene Viewer 4.0.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7267)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    248..377)
                     /label=5'-ITR
                     /note="ApEinfo_fwdcolor=#00ffff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#7eff74
                     vntifkey=21"
     misc_feature    457..2512)
                     /label=human red opsin promoter
                     /note="ApEinfo_fwdcolor=#80ff80
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    2513..2520)
                     /label=NotI
                     /note="ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    2521..2529)
                     /label=Kozak
                     /note="ApEinfo_fwdcolor=#8000ff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    2530..3714)
                     /label=Full CX3CL1 CDS
                     /note="ApEinfo_fwdcolor=#c0c0c0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    3718..3723)
                     /label=AgeI
                     /note="ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    3724..4265)
                     /label=WPRE
                     /note="ApEinfo_fwdcolor=#ffff80
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#7eff74
                     vntifkey=21"
     polyA_signal    4272..4486)
                     /label=BGH pA
                     /label=BGH\pA
                     /note="ApEinfo_fwdcolor=#ff3eee
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff3eee
                     vntifkey=25"
     misc_feature    4487..4492)
                     /label=XhoI
                     /note="ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    4528..4573)
                     /label=additional AAV sequences
                     /label=additional\AAV\sequences
                     /note="ApEinfo_fwdcolor=#7eff74
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#7eff74
                     vntifkey=21"
     misc_feature    4574..4703)
                     /label=3'-ITR
                     /note="ApEinfo_fwdcolor=#00ffff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#7eff74
                     vntifkey=21"
     misc_feature    complement(5467..6324))
                     /label=AmpR
                     /label=misc_marker
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=22"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggctg cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc
      301 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
      361 catcactagg ggttccttgt agttaatgat taacccgcca tgctacttat ctacgtagcc
      421 atgctctagg aagatcggaa ttcgccctta agctagccta cagcagccag ggtgagatta
      481 tgaggctgag ctgagaatat caagactgta ccgagtaggg ggccttggca agtgtggaga
      541 gcccggcagc tggggcagag ggcggagtac ggtgtgcgtt tacggacctc ttcaaacgag
      601 gtaggaaggt cagaagtcaa aaagggaaca aatgatgttt aaccacacaa aaatgaaaat
      661 ccaatggttg gatatccatt ccaaatacac aaaggcaacg gataagtgat ccgggccagg
      721 cacagaaggc catgcacccg taggattgca ctcagagctc ccaaatgcat aggaatagaa
      781 gggtgggtgc aggaggctga ggggtgggga aagggcatgg gtgtttcatg aggacagagc
      841 ttccgtttca tgcaatgaaa agagtttgga gacggatggt ggtgactgga ctatacactt
      901 acacacggta gcgatggtac actttgtatt atgtatattt taccacgatc tttttaaagt
      961 gtcaaaggca aatggccaaa tggttccttg tcctatagct gtagcagcca tcggctgtta
     1021 gtgacaaagc ccctgagtca agatgacagc agcccccata actcctaatc ggctctcccg
     1081 cgtggagtca tttaggagta gtcgcattag agacaagtcc aacatctaat cttccaccct
     1141 ggccagggcc ccagctggca gcgagggtgg gagactccgg gcagagcaga gggcgctgac
     1201 attggggccc ggcctggctt gggtccctct ggcctttccc caggggccct ctttccttgg
     1261 ggctttcttg ggccgccact gctcccgctc ctctcccccc atcccacccc ctcaccccct
     1321 cgttcttcat atccttctct agtgctccct ccactttcat ccacccttct gcaagagtgt
     1381 gggaccacaa atgagttttc acctggcctg gggacacacg tgcccccaca ggtgctgagt
     1441 gactttctag gacagtaatc tgctttaggc taaaatggga cttgatcttc tgttagccct
     1501 aatcatcaat tagcagagcc ggtgaaggtg cagaacctac cgcctttcca ggcctcctcc
     1561 cacctctgcc acctccactc tccttcctgg gatgtggggg ctggcacacg tgtggcccag
     1621 ggcattggtg ggattgcact gagctgggtc attagcgtaa tcctggacaa gggcagacag
     1681 ggcgagcgga gggccagctc cggggctcag gcaaggctgg gggcttcccc cagacacccc
     1741 actcctcctc tgctggaccc ccacttcata gggcacttcg tgttctcaaa gggcttccaa
     1801 atagcatggt ggccttggat gcccagggaa gcctcagagt tgcttatctc cctctagaca
     1861 gaaggggaat ctcggtcaag agggagaggt cgccctgttc aaggccaccc agccagctca
     1921 tggcggtaat gggacaaggc tggccagcca tcccaccctc agaagggacc cggtggggca
     1981 ggtgatctca gaggaggctc acttctgggt ctcacattct tggatccggt tccaggcctc
     2041 ggccctaaat agtctccctg ggctttcaag agaaccacat gagaaaggag gattcgggct
     2101 ctgagcagtt tcaccaccca ccccccagtc tgcaaatcct gacccgtggg tccacctgcc
     2161 ccaaaggcgg acgcaggaca gtagaaggga acagagaaca cataaacaca gagagggcca
     2221 cagcggctcc cacagtcacc gccaccttcc tggcggggat gggtggggcg tctgagtttg
     2281 gttcccagca aatccctctg agccgccctt gcgggctcgc ctcaggagca ggggagcaag
     2341 aggtgggagg aggaggtcta agtcccaggc ccaattaaga gatcaggtag tgtagggttt
     2401 gggagctttt aaggtgaaga ggcccgggct gatcccacag gccagtataa agcgccgtga
     2461 ccctcaggtg atgcgccagg gccggctgcc gtcggggaca gggctttcca tagcggccgc
     2521 gccgccacca tggctccctc gccgctcgcg tggctgctgc gcctggccgc gttcttccat
     2581 ttgtgtactc tgctgccggg tcagcacctc ggcatgacga aatgcgaaat catgtgcgac
     2641 aagatgacct cacgaatccc agtggctttg ctcatccgct atcagctaaa ccaggagtcc
     2701 tgcggcaagc gtgccattgt cctggagacg acacagcaca gacgcttctg tgctgacccg
     2761 aaggagaaat gggtccaaga cgccatgaag catctggatc accaggctgc tgccctcact
     2821 aaaaatggtg gcaagtttga gaagcgggtg gacaatgtga cacctgggat caccttggcc
     2881 actaggggac tgtccccatc tgccctgaca aagcctgaat ccgccacatt ggaagacctt
     2941 gctttggaac tgactactat ttcccaggag gccaggggga ccatggggac ttcccaagag
     3001 ccaccggcag cagtgaccgg atcatctctc tcaacttccg aggcacagga tgcagggctt
     3061 acggctaagc ctcagagcat tggaagtttt gaggcggctg acatctccac caccgtttgg
     3121 ccgagtcctg ctgtctacca atctggatct agctcctggg ctgaggaaaa agctactgag
     3181 tccccctcca ctacagcccc atctcctcag gtgtccacta cttcaccttc aaccccagag
     3241 gaaaatgttg ggtccgaagg ccaaccccca tgggtccagg gacaggacct cagtccagag
     3301 aagtctctag ggtctgagga gataaaccca gttcatactg ataatttcca ggagaggggg
     3361 cctggcaaca cagtccaccc ctcagtggct cccatctcct ctgaagagac ccccagccca
     3421 gagctggtgg cctcgggcag ccaggctcct aagatagagg aacccatcca tgccactgca
     3481 gatccccaga aactgagtgt gcttatcact cctgtccccg acacccaggc agccacaagg
     3541 aggcaggcag tggggctact ggctttcctt ggtcttcttt tctgcctagg ggtggccatg
     3601 tttgcttacc agagccttca gggctgtccc cgcaaaatgg cgggggaaat ggtagaaggc
     3661 ctccgctacg tcccccgtag ctgtggcagt aactcatacg tcctggtgcc agtgtgaacc
     3721 ggtaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3781 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3841 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3901 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3961 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4021 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4081 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     4141 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4201 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4261 cttcgagatc tgcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     4321 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     4381 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     4441 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggactcg agttaagggc
     4501 gaattcccga ttaggatctt cctagagcat ggctacgtag ataagtagca tggcgggtta
     4561 atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     4621 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     4681 tcagtgagcg agcgagcgcg cagccttaat taacctaatt cactggccgt cgttttacaa
     4741 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4801 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4861 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4921 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4981 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5041 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5101 gatggttcac gtagtgggcc atcgccccga tagacggttt ttcgcccttt gacgctggag
     5161 ttcacgttcc tcaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5221 gtctattctt ttgatttata agggattttt ccgatttcgg cctattggtt aaaaaatgag
     5281 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttat aatttcaggt
     5341 ggcatctttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5401 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5461 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5521 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5581 ggtgcacgag tgggttacat cgaactggat ctcaatagtg gtaagatcct tgagagtttt
     5641 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5701 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5761 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5821 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5881 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5941 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     6001 acgatgcctg tagtaatggt aacaacgttg cgcaaactat taactggcga actacttact
     6061 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     6121 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6181 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6241 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6301 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6361 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6421 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6481 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6541 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6601 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6661 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6721 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6781 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6841 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6901 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6961 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7021 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7081 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg cggttttgct
     7141 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7201 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7261 gcggaag
//
