LOCUS       Exported                5796 bp ds-DNA     circular SYN 21-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    AAV-Best1-sCX3CL1.ape
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2020 from SnapGene Viewer 4.0.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5796)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130)
                     /label=5'-ITR
                     /note="ApEinfo_fwdcolor=#0dfff7
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#0dfff7
                     vntifkey=34"
     promoter        211..788)
                     /label=human Best1 promoter
                     /note="ApEinfo_fwdcolor=#346ee0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#346ee0
                     vntifkey=29"
     intron          826..958)
                     /label=SV40 misc intron (Promega)
                     /label=SV40\misc\intron\(Promega)
                     /note="ApEinfo_fwdcolor=#eb606c
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#eb606c
                     vntifkey=15"
     misc_feature    966..973)
                     /label=NotI
                     /note="ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    974..982)
                     /label=Kozak
                     /note="ApEinfo_fwdcolor=#80ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    983..1990)
                     /label=Soluble CX3CL1 CDS
                     /note="ApEinfo_fwdcolor=#ff80ff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    1994..1999)
                     /label=HindIII
                     /note="ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    2006..2547)
                     /label=WPRE
                     /note="ApEinfo_fwdcolor=#7eff74
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#7eff74
                     vntifkey=21"
     polyA_signal    2554..2768)
                     /label=BGH pA
                     /label=BGH\pA
                     /note="ApEinfo_fwdcolor=#ff3eee
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff3eee
                     vntifkey=25"
     misc_feature    2769..2774)
                     /label=XhoI
                     /note="ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     repeat_region   2856..2985)
                     /label=3'-ITR
                     /note="ApEinfo_fwdcolor=#0dfff7
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#0dfff7
                     vntifkey=34"
     CDS             3749..4609)
                     /label=Amp-R
                     /note="ApEinfo_fwdcolor=#e9d024
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#e9d024
                     vntifkey=4"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcgccc ttaagctagc tccttttcag ataagggcac tgaggctgag
      241 agaggagctg aaacctaccc ggggtcacca cacacaggtg gcaaggctgg gaccagaaac
      301 caggactgtt gactgcagcc cggtattcat tctttccata gcccacaggg ctgtcaaaga
      361 ccccagggcc tagtcagagg ctcctccttc ctggagagtt cctggcacag aagttgaagc
      421 tcagcacagc cccctaaccc ccaactctct ctgcaaggcc tcaggggtca gaacactggt
      481 ggagcagatc ctttagcctc tggattttag ggccatggta gagggggtgt tgccctaaat
      541 tccagccctg gtctcagccc aacaccctcc aagaagaaat tagaggggcc atggccaggc
      601 tgtgctagcc gttgcttctg agcagattac aagaagggac taagacaagg actcctttgt
      661 ggaggtcctg gcttagggag tcaagtgacg gcggctcagc actcacgtgg gcagtgccag
      721 cctctaagag tgggcagggg cactggccac agagtcccag ggagtcccac cagcctagtc
      781 gccagaccca gatcctgcag aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt
      841 acaagacagg tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg
      901 cgtttctgat aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt
      961 gtccagcggc cgcgccgcca ccatggctcc ctcgccgctc gcgtggctgc tgcgcctggc
     1021 cgcgttcttc catttgtgta ctctgctgcc gggtcagcac ctcggcatga cgaaatgcga
     1081 aatcatgtgc gacaagatga cctcacgaat cccagtggct ttgctcatcc gctatcagct
     1141 aaaccaggag tcctgcggca agcgtgccat tgtcctggag acgacacagc acagacgctt
     1201 ctgtgctgac ccgaaggaga aatgggtcca agacgccatg aagcatctgg atcaccaggc
     1261 tgctgccctc actaaaaatg gtggcaagtt tgagaagcgg gtggacaatg tgacacctgg
     1321 gatcaccttg gccactaggg gactgtcccc atctgccctg acaaagcctg aatccgccac
     1381 attggaagac cttgctttgg aactgactac tatttcccag gaggccaggg ggaccatggg
     1441 gacttcccaa gagccaccgg cagcagtgac cggatcatct ctctcaactt ccgaggcaca
     1501 ggatgcaggg cttacggcta agcctcagag cattggaagt tttgaggcgg ctgacatctc
     1561 caccaccgtt tggccgagtc ctgctgtcta ccaatctgga tctagctcct gggctgagga
     1621 aaaagctact gagtccccct ccactacagc cccatctcct caggtgtcca ctacttcacc
     1681 ttcaacccca gaggaaaatg ttgggtccga aggccaaccc ccatgggtcc agggacagga
     1741 cctcagtcca gagaagtctc tagggtctga ggagataaac ccagttcata ctgataattt
     1801 ccaggagagg gggcctggca acacagtcca cccctcagtg gctcccatct cctctgaaga
     1861 gacccccagc ccagagctgg tggcctcggg cagccaggct cctaagatag aggaacccat
     1921 ccatgccact gcagatcccc agaaactgag tgtgcttatc actcctgtcc ccgacaccca
     1981 ggcagccaca tgaaagcttg gatccaatca acctctggat tacaaaattt gtgaaagatt
     2041 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     2101 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     2161 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     2221 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     2281 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     2341 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     2401 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     2461 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     2521 ggctctgcgg cctcttccgc gtcttcgaga tctgcctcga ctgtgccttc tagttgccag
     2581 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     2641 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     2701 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     2761 gctggggact cgagttaagg gcgaattccc gataaggatc ttcctagagc atggctacgt
     2821 agataagtag catggcgggt taatcattaa ctacaaggaa cccctagtga tggagttggc
     2881 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     2941 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagcctta attaacctaa
     3001 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     3061 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     3121 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     3181 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3241 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3301 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3361 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3421 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3481 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     3541 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     3601 attaacgttt ataatttcag gtggcatctt tcggggaaat gtgcgcggaa cccctatttg
     3661 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3721 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3781 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3841 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaatag
     3901 tggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3961 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4021 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4081 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4141 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4201 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4261 accaaacgac gagcgtgaca ccacgatgcc tgtagtaatg gtaacaacgt tgcgcaaact
     4321 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4381 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4441 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4501 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4561 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4621 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4681 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4741 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4801 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4861 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4921 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4981 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5041 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5101 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5161 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5221 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5281 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5341 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5401 ggccttttgc tgcggttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5461 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     5521 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     5581 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     5641 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     5701 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     5761 cagctatgac catgattacg ccagatttaa ttaagg
//
