LOCUS       Exported                7316 bp ds-DNA     circular SYN 21-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    AAV-hRO-TGFB2.ape
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2020 from SnapGene Viewer 4.0.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7316)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    248..377)
                     /label=5'-ITR
                     /note="ApEinfo_fwdcolor=#7eff74
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#7eff74
                     vntifkey=21"
     misc_feature    457..2512)
                     /label=human red opsin promoter
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    2513..2520)
                     /label=NotI
                     /note="ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    2522..3763)
                     /label=Tgfb2
                     /note="ApEinfo_fwdcolor=#ffff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    3767..3772)
                     /label=AgeI
                     /note="ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    3773..4314)
                     /label=WPRE
                     /note="ApEinfo_fwdcolor=#c0c0c0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#7eff74
                     vntifkey=21"
     polyA_signal    4321..4535)
                     /label=BGH pA
                     /label=BGH\pA
                     /note="ApEinfo_fwdcolor=#ff3eee
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff3eee
                     vntifkey=25"
     misc_feature    4536..4541)
                     /label=XhoI
                     /note="ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    4577..4622)
                     /label=additional AAV sequences
                     /label=additional\AAV\sequences
                     /note="ApEinfo_fwdcolor=#7eff74
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#7eff74
                     vntifkey=21"
     misc_feature    4623..4752)
                     /label=3'-ITR
                     /note="ApEinfo_fwdcolor=#7eff74
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#7eff74
                     vntifkey=21"
     misc_feature    complement(5516..6373))
                     /label=AmpR
                     /label=misc_marker
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=22"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggctg cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc
      301 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
      361 catcactagg ggttccttgt agttaatgat taacccgcca tgctacttat ctacgtagcc
      421 atgctctagg aagatcggaa ttcgccctta agctagccta cagcagccag ggtgagatta
      481 tgaggctgag ctgagaatat caagactgta ccgagtaggg ggccttggca agtgtggaga
      541 gcccggcagc tggggcagag ggcggagtac ggtgtgcgtt tacggacctc ttcaaacgag
      601 gtaggaaggt cagaagtcaa aaagggaaca aatgatgttt aaccacacaa aaatgaaaat
      661 ccaatggttg gatatccatt ccaaatacac aaaggcaacg gataagtgat ccgggccagg
      721 cacagaaggc catgcacccg taggattgca ctcagagctc ccaaatgcat aggaatagaa
      781 gggtgggtgc aggaggctga ggggtgggga aagggcatgg gtgtttcatg aggacagagc
      841 ttccgtttca tgcaatgaaa agagtttgga gacggatggt ggtgactgga ctatacactt
      901 acacacggta gcgatggtac actttgtatt atgtatattt taccacgatc tttttaaagt
      961 gtcaaaggca aatggccaaa tggttccttg tcctatagct gtagcagcca tcggctgtta
     1021 gtgacaaagc ccctgagtca agatgacagc agcccccata actcctaatc ggctctcccg
     1081 cgtggagtca tttaggagta gtcgcattag agacaagtcc aacatctaat cttccaccct
     1141 ggccagggcc ccagctggca gcgagggtgg gagactccgg gcagagcaga gggcgctgac
     1201 attggggccc ggcctggctt gggtccctct ggcctttccc caggggccct ctttccttgg
     1261 ggctttcttg ggccgccact gctcccgctc ctctcccccc atcccacccc ctcaccccct
     1321 cgttcttcat atccttctct agtgctccct ccactttcat ccacccttct gcaagagtgt
     1381 gggaccacaa atgagttttc acctggcctg gggacacacg tgcccccaca ggtgctgagt
     1441 gactttctag gacagtaatc tgctttaggc taaaatggga cttgatcttc tgttagccct
     1501 aatcatcaat tagcagagcc ggtgaaggtg cagaacctac cgcctttcca ggcctcctcc
     1561 cacctctgcc acctccactc tccttcctgg gatgtggggg ctggcacacg tgtggcccag
     1621 ggcattggtg ggattgcact gagctgggtc attagcgtaa tcctggacaa gggcagacag
     1681 ggcgagcgga gggccagctc cggggctcag gcaaggctgg gggcttcccc cagacacccc
     1741 actcctcctc tgctggaccc ccacttcata gggcacttcg tgttctcaaa gggcttccaa
     1801 atagcatggt ggccttggat gcccagggaa gcctcagagt tgcttatctc cctctagaca
     1861 gaaggggaat ctcggtcaag agggagaggt cgccctgttc aaggccaccc agccagctca
     1921 tggcggtaat gggacaaggc tggccagcca tcccaccctc agaagggacc cggtggggca
     1981 ggtgatctca gaggaggctc acttctgggt ctcacattct tggatccggt tccaggcctc
     2041 ggccctaaat agtctccctg ggctttcaag agaaccacat gagaaaggag gattcgggct
     2101 ctgagcagtt tcaccaccca ccccccagtc tgcaaatcct gacccgtggg tccacctgcc
     2161 ccaaaggcgg acgcaggaca gtagaaggga acagagaaca cataaacaca gagagggcca
     2221 cagcggctcc cacagtcacc gccaccttcc tggcggggat gggtggggcg tctgagtttg
     2281 gttcccagca aatccctctg agccgccctt gcgggctcgc ctcaggagca ggggagcaag
     2341 aggtgggagg aggaggtcta agtcccaggc ccaattaaga gatcaggtag tgtagggttt
     2401 gggagctttt aaggtgaaga ggcccgggct gatcccacag gccagtataa agcgccgtga
     2461 ccctcaggtg atgcgccagg gccggctgcc gtcggggaca gggctttcca tagcggccgc
     2521 catgcactac tgtgtgctga gcaccttttt gctcctgcat ctggtcccgg tggcgctcag
     2581 tctgtctacc tgcagcaccc tcgacatgga tcagtttatg cgcaagagga tcgaggccat
     2641 ccgcgggcag atcctgagca agctgaagct caccagcccc ccggaagact atccggagcc
     2701 ggatgaggtc cccccggagg tgatttccat ctacaacagt accagggact tactgcagga
     2761 gaaggcaagc cggagggcag ccgcctgcga gcgcgagcgg agcgacgagg agtactacgc
     2821 caaggaggtt tataaaatcg acatgccgtc ccacctcccc tccgaaaatg ccatcccgcc
     2881 cactttctac agaccctact tcagaatcgt ccgctttgat gtctcaacaa tggagaaaaa
     2941 tgcttcgaat ctggtgaagg cagagttcag ggtcttccgc ttgcaaaacc ccaaagccag
     3001 agtggccgag cagcggattg aactgtatca gatccttaaa tccaaagact taacatctcc
     3061 cacccagcgc tacatcgata gcaaggttgt gaaaaccaga gcggagggtg aatggctctc
     3121 cttcgacgtg acagacgctg tgcaggagtg gcttcaccac aaagacagga acctggggtt
     3181 taaaataagt ttacactgcc cctgctgtac cttcgtgccg tctaataatt acatcatccc
     3241 gaataaaagc gaagagctcg aggcgagatt tgcaggtatt gatggcacct ctacatatgc
     3301 cagtggtgat cagaaaacta taaagtccac taggaaaaaa accagtggga agaccccaca
     3361 tctcctgcta atgttgttgc cctcctacag actggagtca caacagtcca gccggcggaa
     3421 gaagcgcgct ttggatgctg cctactgctt tagaaatgtg caggataatt gctgccttcg
     3481 ccctctttac attgatttta agagggatct tggatggaaa tggatccatg aacccaaagg
     3541 gtacaatgct aacttctgtg ctggggcatg cccatatcta tggagttcag acactcaaca
     3601 caccaaagtc ctcagcctgt acaacaccat aaatcccgaa gcttccgctt ccccttgctg
     3661 tgtgtcccag gatctggaac cactgaccat tctctattac attggaaata cgcccaagat
     3721 cgaacagctt tccaatatga ttgtcaagtc ttgtaaatgc agctaaaccg gtaatcaacc
     3781 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3841 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3901 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     3961 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4021 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4081 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4141 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     4201 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4261 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgagatct
     4321 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     4381 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     4441 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     4501 gaggattggg aagacaatag caggcatgct ggggactcga gttaagggcg aattcccgat
     4561 taggatcttc ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta
     4621 caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     4681 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     4741 gcgagcgcgc agccttaatt aacctaattc actggccgtc gttttacaac gtcgtgactg
     4801 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     4861 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     4921 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4981 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5041 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     5101 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     5161 tagtgggcca tcgccccgat agacggtttt tcgccctttg acgctggagt tcacgttcct
     5221 caatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     5281 tgatttataa gggatttttc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     5341 aaaatttaac gcgaatttta acaaaatatt aacgtttata atttcaggtg gcatctttcg
     5401 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5461 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5521 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5581 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5641 gggttacatc gaactggatc tcaatagtgg taagatcctt gagagttttc gccccgaaga
     5701 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5761 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5821 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5881 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5941 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6001 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     6061 agtaatggta acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6121 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6181 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6241 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6301 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6361 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6421 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6481 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6541 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6601 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6661 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     6721 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6781 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6841 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6901 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6961 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7021 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7081 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7141 cagcaacgcg gcctttttac ggttcctggc cttttgctgc ggttttgctc acatgttctt
     7201 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7261 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
