LOCUS Exported 7310 bp ds-DNA circular SYN 21-DEC-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS AAV-hRO-TGFB3.ape SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7310) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 21, 2020 from SnapGene Viewer 4.0.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7310) /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 248..377) /label=5'-ITR /note="ApEinfo_fwdcolor=#7eff74 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=#7eff74 vntifkey=21" misc_feature 457..2512) /label=human red opsin promoter /note="ApEinfo_fwdcolor=cyan ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=green" misc_feature 2513..2520) /label=NotI /note="ApEinfo_fwdcolor=#ff0000 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=green" misc_feature 2522..3757) /label=Tgfb3 /note="ApEinfo_fwdcolor=#8080ff ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=green" misc_feature 3761..3766) /label=AgeI /note="ApEinfo_fwdcolor=#ff0000 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=green" misc_feature 3767..4308) /label=WPRE /note="ApEinfo_fwdcolor=#c0c0c0 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=#7eff74 vntifkey=21" polyA_signal 4315..4529) /label=BGH pA /label=BGH\pA /note="ApEinfo_fwdcolor=#ff3eee ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=#ff3eee vntifkey=25" misc_feature 4530..4535) /label=XhoI /note="ApEinfo_fwdcolor=#ff0000 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=green" misc_feature 4571..4616) /label=additional AAV sequences /label=additional\AAV\sequences /note="ApEinfo_fwdcolor=#7eff74 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=#7eff74 vntifkey=21" misc_feature 4617..4746) /label=3'-ITR /note="ApEinfo_fwdcolor=#7eff74 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=#7eff74 vntifkey=21" misc_feature complement(5510..6367)) /label=AmpR /label=misc_marker /note="ApEinfo_fwdcolor=pink ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=pink vntifkey=22" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta 241 attaaggctg cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc 301 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc 361 catcactagg ggttccttgt agttaatgat taacccgcca tgctacttat ctacgtagcc 421 atgctctagg aagatcggaa ttcgccctta agctagccta cagcagccag ggtgagatta 481 tgaggctgag ctgagaatat caagactgta ccgagtaggg ggccttggca agtgtggaga 541 gcccggcagc tggggcagag ggcggagtac ggtgtgcgtt tacggacctc ttcaaacgag 601 gtaggaaggt cagaagtcaa aaagggaaca aatgatgttt aaccacacaa aaatgaaaat 661 ccaatggttg gatatccatt ccaaatacac aaaggcaacg gataagtgat ccgggccagg 721 cacagaaggc catgcacccg taggattgca ctcagagctc ccaaatgcat aggaatagaa 781 gggtgggtgc aggaggctga ggggtgggga aagggcatgg gtgtttcatg aggacagagc 841 ttccgtttca tgcaatgaaa agagtttgga gacggatggt ggtgactgga ctatacactt 901 acacacggta gcgatggtac actttgtatt atgtatattt taccacgatc tttttaaagt 961 gtcaaaggca aatggccaaa tggttccttg tcctatagct gtagcagcca tcggctgtta 1021 gtgacaaagc ccctgagtca agatgacagc agcccccata actcctaatc ggctctcccg 1081 cgtggagtca tttaggagta gtcgcattag agacaagtcc aacatctaat cttccaccct 1141 ggccagggcc ccagctggca gcgagggtgg gagactccgg gcagagcaga gggcgctgac 1201 attggggccc ggcctggctt gggtccctct ggcctttccc caggggccct ctttccttgg 1261 ggctttcttg ggccgccact gctcccgctc ctctcccccc atcccacccc ctcaccccct 1321 cgttcttcat atccttctct agtgctccct ccactttcat ccacccttct gcaagagtgt 1381 gggaccacaa atgagttttc acctggcctg gggacacacg tgcccccaca ggtgctgagt 1441 gactttctag gacagtaatc tgctttaggc taaaatggga cttgatcttc tgttagccct 1501 aatcatcaat tagcagagcc ggtgaaggtg cagaacctac cgcctttcca ggcctcctcc 1561 cacctctgcc acctccactc tccttcctgg gatgtggggg ctggcacacg tgtggcccag 1621 ggcattggtg ggattgcact gagctgggtc attagcgtaa tcctggacaa gggcagacag 1681 ggcgagcgga gggccagctc cggggctcag gcaaggctgg gggcttcccc cagacacccc 1741 actcctcctc tgctggaccc ccacttcata gggcacttcg tgttctcaaa gggcttccaa 1801 atagcatggt ggccttggat gcccagggaa gcctcagagt tgcttatctc cctctagaca 1861 gaaggggaat ctcggtcaag agggagaggt cgccctgttc aaggccaccc agccagctca 1921 tggcggtaat gggacaaggc tggccagcca tcccaccctc agaagggacc cggtggggca 1981 ggtgatctca gaggaggctc acttctgggt ctcacattct tggatccggt tccaggcctc 2041 ggccctaaat agtctccctg ggctttcaag agaaccacat gagaaaggag gattcgggct 2101 ctgagcagtt tcaccaccca ccccccagtc tgcaaatcct gacccgtggg tccacctgcc 2161 ccaaaggcgg acgcaggaca gtagaaggga acagagaaca cataaacaca gagagggcca 2221 cagcggctcc cacagtcacc gccaccttcc tggcggggat gggtggggcg tctgagtttg 2281 gttcccagca aatccctctg agccgccctt gcgggctcgc ctcaggagca ggggagcaag 2341 aggtgggagg aggaggtcta agtcccaggc ccaattaaga gatcaggtag tgtagggttt 2401 gggagctttt aaggtgaaga ggcccgggct gatcccacag gccagtataa agcgccgtga 2461 ccctcaggtg atgcgccagg gccggctgcc gtcggggaca gggctttcca tagcggccgc 2521 catgaagatg cacttgcaaa gggctctggt agtcctggcc ctgctgaact tggccacaat 2581 cagcctctct ctgtccactt gcaccacgtt ggacttcggc cacatcaaga agaagagggt 2641 ggaagccatt aggggacaga tcttgagcaa gctcaggctc accagccccc ctgagccatc 2701 ggtgatgacc cacgtcccct atcaggtcct ggcactttac aacagcaccc gggagttgct 2761 ggaagagatg cacggggaga gggaggaagg ctgcactcag gagacctcgg agtctgagta 2821 ctatgccaaa gagatccata aattcgacat gatccaggga ctggcggagc acaatgaact 2881 ggccgtctgc cccaaaggaa ttacctctaa ggtttttcgt ttcaatgtgt cctcagtgga 2941 gaaaaatgga accaatctgt tccgggcaga gttccgggtc ttgcgggtgc ccaaccccag 3001 ctccaagcgc acagagcaga gaattgagct cttccagata cttcgaccgg atgagcacat 3061 agccaagcag cgctacatag gtggcaagaa tctgcccaca aggggcaccg ctgaatggct 3121 gtctttcgat gtcactgaca ctgtgcgcga gtggctgttg aggagagagt ccaacttggg 3181 tctggaaatc agcatccact gtccatgtca cacctttcag cccaatggag acatactgga 3241 aaatgttcat gaggtgatgg aaatcaaatt caaaggagtg gacaatgaag atgaccatgg 3301 ccgtggagac ctggggcgtc tcaagaagca aaaggatcac cacaacccac acctgatcct 3361 catgatgatc cccccacacc gactggacag cccaggccag ggcagtcaga ggaagaagag 3421 ggccctggac accaattact gcttccgcaa cctggaggag aactgctgtg tacgccccct 3481 ttatattgac ttccggcagg atctaggctg gaaatgggtc cacgaaccta agggttacta 3541 tgccaacttc tgctcaggcc cttgcccata cctccgcagc gcagacacaa cccatagcac 3601 ggtgcttgga ctatacaaca ccctgaaccc agaggcgtct gcctcgccat gctgcgtccc 3661 ccaggacctg gagcccctga ccatcttgta ctatgtgggc agaaccccca aggtggagca 3721 gctgtccaac atggtggtga agtcgtgtaa gtgcagctga accggtaatc aacctctgga 3781 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 3841 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 3901 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 3961 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 4021 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 4081 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 4141 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 4201 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 4261 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgag atctgcctcg 4321 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 4381 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 4441 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 4501 tgggaagaca atagcaggca tgctggggac tcgagttaag ggcgaattcc cgattaggat 4561 cttcctagag catggctacg tagataagta gcatggcggg ttaatcatta actacaagga 4621 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg 4681 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc 4741 gcgcagcctt aattaaccta attcactggc cgtcgtttta caacgtcgtg actgggaaaa 4801 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 4861 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 4921 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 4981 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 5041 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 5101 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 5161 gccatcgccc cgatagacgg tttttcgccc tttgacgctg gagttcacgt tcctcaatag 5221 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 5281 ataagggatt tttccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 5341 taacgcgaat tttaacaaaa tattaacgtt tataatttca ggtggcatct ttcggggaaa 5401 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 5461 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 5521 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 5581 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta 5641 catcgaactg gatctcaata gtggtaagat ccttgagagt tttcgccccg aagaacgttt 5701 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc 5761 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 5821 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 5881 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 5941 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 6001 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagtaat 6061 ggtaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 6121 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 6181 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat 6241 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 6301 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 6361 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 6421 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 6481 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 6541 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 6601 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 6661 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 6721 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 6781 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 6841 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 6901 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 6961 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 7021 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 7081 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 7141 cgcggccttt ttacggttcc tggccttttg ctgcggtttt gctcacatgt tctttcctgc 7201 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 7261 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag //