LOCUS       Exported                7310 bp ds-DNA     circular SYN 21-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    AAV-hRO-TGFB3.ape
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2020 from SnapGene Viewer 4.0.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7310)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    248..377)
                     /label=5'-ITR
                     /note="ApEinfo_fwdcolor=#7eff74
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#7eff74
                     vntifkey=21"
     misc_feature    457..2512)
                     /label=human red opsin promoter
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    2513..2520)
                     /label=NotI
                     /note="ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    2522..3757)
                     /label=Tgfb3
                     /note="ApEinfo_fwdcolor=#8080ff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    3761..3766)
                     /label=AgeI
                     /note="ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    3767..4308)
                     /label=WPRE
                     /note="ApEinfo_fwdcolor=#c0c0c0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#7eff74
                     vntifkey=21"
     polyA_signal    4315..4529)
                     /label=BGH pA
                     /label=BGH\pA
                     /note="ApEinfo_fwdcolor=#ff3eee
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff3eee
                     vntifkey=25"
     misc_feature    4530..4535)
                     /label=XhoI
                     /note="ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    4571..4616)
                     /label=additional AAV sequences
                     /label=additional\AAV\sequences
                     /note="ApEinfo_fwdcolor=#7eff74
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#7eff74
                     vntifkey=21"
     misc_feature    4617..4746)
                     /label=3'-ITR
                     /note="ApEinfo_fwdcolor=#7eff74
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#7eff74
                     vntifkey=21"
     misc_feature    complement(5510..6367))
                     /label=AmpR
                     /label=misc_marker
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=22"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggctg cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc
      301 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
      361 catcactagg ggttccttgt agttaatgat taacccgcca tgctacttat ctacgtagcc
      421 atgctctagg aagatcggaa ttcgccctta agctagccta cagcagccag ggtgagatta
      481 tgaggctgag ctgagaatat caagactgta ccgagtaggg ggccttggca agtgtggaga
      541 gcccggcagc tggggcagag ggcggagtac ggtgtgcgtt tacggacctc ttcaaacgag
      601 gtaggaaggt cagaagtcaa aaagggaaca aatgatgttt aaccacacaa aaatgaaaat
      661 ccaatggttg gatatccatt ccaaatacac aaaggcaacg gataagtgat ccgggccagg
      721 cacagaaggc catgcacccg taggattgca ctcagagctc ccaaatgcat aggaatagaa
      781 gggtgggtgc aggaggctga ggggtgggga aagggcatgg gtgtttcatg aggacagagc
      841 ttccgtttca tgcaatgaaa agagtttgga gacggatggt ggtgactgga ctatacactt
      901 acacacggta gcgatggtac actttgtatt atgtatattt taccacgatc tttttaaagt
      961 gtcaaaggca aatggccaaa tggttccttg tcctatagct gtagcagcca tcggctgtta
     1021 gtgacaaagc ccctgagtca agatgacagc agcccccata actcctaatc ggctctcccg
     1081 cgtggagtca tttaggagta gtcgcattag agacaagtcc aacatctaat cttccaccct
     1141 ggccagggcc ccagctggca gcgagggtgg gagactccgg gcagagcaga gggcgctgac
     1201 attggggccc ggcctggctt gggtccctct ggcctttccc caggggccct ctttccttgg
     1261 ggctttcttg ggccgccact gctcccgctc ctctcccccc atcccacccc ctcaccccct
     1321 cgttcttcat atccttctct agtgctccct ccactttcat ccacccttct gcaagagtgt
     1381 gggaccacaa atgagttttc acctggcctg gggacacacg tgcccccaca ggtgctgagt
     1441 gactttctag gacagtaatc tgctttaggc taaaatggga cttgatcttc tgttagccct
     1501 aatcatcaat tagcagagcc ggtgaaggtg cagaacctac cgcctttcca ggcctcctcc
     1561 cacctctgcc acctccactc tccttcctgg gatgtggggg ctggcacacg tgtggcccag
     1621 ggcattggtg ggattgcact gagctgggtc attagcgtaa tcctggacaa gggcagacag
     1681 ggcgagcgga gggccagctc cggggctcag gcaaggctgg gggcttcccc cagacacccc
     1741 actcctcctc tgctggaccc ccacttcata gggcacttcg tgttctcaaa gggcttccaa
     1801 atagcatggt ggccttggat gcccagggaa gcctcagagt tgcttatctc cctctagaca
     1861 gaaggggaat ctcggtcaag agggagaggt cgccctgttc aaggccaccc agccagctca
     1921 tggcggtaat gggacaaggc tggccagcca tcccaccctc agaagggacc cggtggggca
     1981 ggtgatctca gaggaggctc acttctgggt ctcacattct tggatccggt tccaggcctc
     2041 ggccctaaat agtctccctg ggctttcaag agaaccacat gagaaaggag gattcgggct
     2101 ctgagcagtt tcaccaccca ccccccagtc tgcaaatcct gacccgtggg tccacctgcc
     2161 ccaaaggcgg acgcaggaca gtagaaggga acagagaaca cataaacaca gagagggcca
     2221 cagcggctcc cacagtcacc gccaccttcc tggcggggat gggtggggcg tctgagtttg
     2281 gttcccagca aatccctctg agccgccctt gcgggctcgc ctcaggagca ggggagcaag
     2341 aggtgggagg aggaggtcta agtcccaggc ccaattaaga gatcaggtag tgtagggttt
     2401 gggagctttt aaggtgaaga ggcccgggct gatcccacag gccagtataa agcgccgtga
     2461 ccctcaggtg atgcgccagg gccggctgcc gtcggggaca gggctttcca tagcggccgc
     2521 catgaagatg cacttgcaaa gggctctggt agtcctggcc ctgctgaact tggccacaat
     2581 cagcctctct ctgtccactt gcaccacgtt ggacttcggc cacatcaaga agaagagggt
     2641 ggaagccatt aggggacaga tcttgagcaa gctcaggctc accagccccc ctgagccatc
     2701 ggtgatgacc cacgtcccct atcaggtcct ggcactttac aacagcaccc gggagttgct
     2761 ggaagagatg cacggggaga gggaggaagg ctgcactcag gagacctcgg agtctgagta
     2821 ctatgccaaa gagatccata aattcgacat gatccaggga ctggcggagc acaatgaact
     2881 ggccgtctgc cccaaaggaa ttacctctaa ggtttttcgt ttcaatgtgt cctcagtgga
     2941 gaaaaatgga accaatctgt tccgggcaga gttccgggtc ttgcgggtgc ccaaccccag
     3001 ctccaagcgc acagagcaga gaattgagct cttccagata cttcgaccgg atgagcacat
     3061 agccaagcag cgctacatag gtggcaagaa tctgcccaca aggggcaccg ctgaatggct
     3121 gtctttcgat gtcactgaca ctgtgcgcga gtggctgttg aggagagagt ccaacttggg
     3181 tctggaaatc agcatccact gtccatgtca cacctttcag cccaatggag acatactgga
     3241 aaatgttcat gaggtgatgg aaatcaaatt caaaggagtg gacaatgaag atgaccatgg
     3301 ccgtggagac ctggggcgtc tcaagaagca aaaggatcac cacaacccac acctgatcct
     3361 catgatgatc cccccacacc gactggacag cccaggccag ggcagtcaga ggaagaagag
     3421 ggccctggac accaattact gcttccgcaa cctggaggag aactgctgtg tacgccccct
     3481 ttatattgac ttccggcagg atctaggctg gaaatgggtc cacgaaccta agggttacta
     3541 tgccaacttc tgctcaggcc cttgcccata cctccgcagc gcagacacaa cccatagcac
     3601 ggtgcttgga ctatacaaca ccctgaaccc agaggcgtct gcctcgccat gctgcgtccc
     3661 ccaggacctg gagcccctga ccatcttgta ctatgtgggc agaaccccca aggtggagca
     3721 gctgtccaac atggtggtga agtcgtgtaa gtgcagctga accggtaatc aacctctgga
     3781 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     3841 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     3901 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     3961 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     4021 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     4081 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     4141 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     4201 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     4261 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgag atctgcctcg
     4321 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4381 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4441 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4501 tgggaagaca atagcaggca tgctggggac tcgagttaag ggcgaattcc cgattaggat
     4561 cttcctagag catggctacg tagataagta gcatggcggg ttaatcatta actacaagga
     4621 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     4681 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     4741 gcgcagcctt aattaaccta attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     4801 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     4861 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     4921 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4981 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5041 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5101 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5161 gccatcgccc cgatagacgg tttttcgccc tttgacgctg gagttcacgt tcctcaatag
     5221 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5281 ataagggatt tttccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5341 taacgcgaat tttaacaaaa tattaacgtt tataatttca ggtggcatct ttcggggaaa
     5401 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5461 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5521 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5581 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5641 catcgaactg gatctcaata gtggtaagat ccttgagagt tttcgccccg aagaacgttt
     5701 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5761 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5821 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5881 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5941 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6001 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagtaat
     6061 ggtaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6121 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6181 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6241 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6301 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6361 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6421 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6481 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6541 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6601 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6661 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     6721 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6781 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6841 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6901 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6961 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7021 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7081 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7141 cgcggccttt ttacggttcc tggccttttg ctgcggtttt gctcacatgt tctttcctgc
     7201 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7261 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
//
