LOCUS       2822_-_HDM-SARS-CoV-2deltaC18        8334 bp    DNA     circular UNA 21-APR-2020
DEFINITION  .
ACCESSION   urn.local.alexbalazs.n-bx0dw73
VERSION     urn.local.alexbalazs.n-bx0dw73
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    9..679
                     /Description="CMV promoter.  Transcription starts with
                     next base.  5 prime end represents complete extent of the
                     CMV promoter in MDM based plasmids, which corresponds to
                     positions 471..1141 of hCMVie genbank record accession
                     X03922."
                     /label="CMV"
                     /note="Geneious type: promoter prokaryotic"
     misc_feature    96..679
                     /Description="CMV promoter.  Transcription starts with
                     next base.  5 prime end starts at Spe I site only.  The
                     CMV region in this construct may extend further upstream."
                     /label="CMV"
                     /note="Geneious type: promoter prokaryotic"
     primer_bind     151..178
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     638 bases.  Perfect match Tm = 58.7 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is agttccgcgt tacataactt acggtaaa."
                     /label="HGTI-1832_Max=638"
     primer_bind     complement(236..260)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     672 bases.  Perfect match Tm = 59.1 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ccctattggc gttactatgg gaaca."
                     /label="HGTI-1863_Max=672"
     primer_bind     complement(536..560)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     485 bases.  Perfect match Tm = 58.0 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is aaacaaactc ccattgacgt caatg."
                     /label="HGTI-1864_Max=485"
     primer_bind     561..593
                     /Description="sequencing pHD from 3xx end  Max recorded
                     sequencing read = 707 bases. Perfect match Tm = 71.5 C,
                     although only 17 nt are required to match.  The actual
                     sequence of the primer is tggcaccaaa atcaacggga ctttccaaaa
                     tgt."
                     /label="HGTI-2021_Max=707"
     primer_bind     612..652
                     /Description="?  Max recorded sequencing read = 162 bases.
                      Perfect match Tm = 76.1 C, although only 17 nt are
                     required to match.  The actual sequence of the primer is
                     gcgcactagt catatggtag gcgtgtacgg tgggaggtct a."
                     /label="HGTI-1220_Max=162"
     primer_bind     617..638
                     /Description="Sequencing primers for pHJ1  Max recorded
                     sequencing read = 311 bases. Perfect match Tm = 62.5 C,
                     although only 17 nt are required to match.  The actual
                     sequence of the primer is GCAAATGGGC GGTAGGCGTG TA."
                     /label="HGTI-1204_Max=311"
     misc_feature    628..679
                     /Description="CMV TATA box sequences, from -52 to -1. 
                     Wild type, with Sac I site."
                     /label="CMV_TATA"
     primer_bind     complement(662..703)
                     /Description="linker from SacI of CMV bridging into BglII
                     of HIV R.  Max recorded sequencing read =  bases.  Perfect
                     match Tm = 74.8 C, although only 17 nt are required to
                     match.  The actual sequence of the primer is gatcTGGTCT
                     AACCAGAGAG ACCCcggttc actaaacgAG CT."
                     /label="HGTI-1192_Max="
     misc_feature    680..750
                     /Description="5xx untranslated region from CMV immediate
                     early promoter.  The 3 prime end corresponds to the full
                     extent of CMV sequences in the MDM constructs."
                     /label="CMV_5xxUTR"
     primer_bind     692..715
                     /Description="PCR or sequencing primer located in CMV
                     sequence just after 5xx end of RNA transcript.  Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     63.9 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GAGACGCCAT CCACGCTGTT
                     TTGA."
                     /label="HGTI-761_Max="
     primer_bind     751..775
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     595 bases.  Perfect match Tm = 58.7 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is cctcgaagct gatcctgaga acttc."
                     /label="HGTI-1834_Max=595"
     misc_feature    779..1254
                     /Description="Human B-globin 2nd intron, Rsa I deleted."
                     /label="B-globin_intron"
     primer_bind     complement(833..860)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     636 bases.  Perfect match Tm = 59.3 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is tgttacttct ccccttccta tgacatga."
                     /label="HGTI-1865_Max=636"
     primer_bind     complement(952..981)
                     /Description="sequencing primer in globin intron facing
                     left.  Max recorded sequencing read = 610 bases.  Perfect
                     match Tm = 53.9 C, although only 17 nt are required to
                     match.  The actual sequence of the primer is gaaagaaaga
                     gattagggaa agtattagaa ."
                     /label="HGTI-2022_Max=610"
     primer_bind     1051..1082
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     639 bases.  Perfect match Tm = 58.2 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is gttaaggcaa tagcaatatt tctgcatata aa."
                     /label="HGTI-1835_Max=639"
     primer_bind     complement(1135..1160)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     639 bases.  Perfect match Tm = 59.3 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is gcagaatggt agctggattg tagctg."
                     /label="HGTI-1866_Max=639"
     primer_bind     1209..1243
                     /Description="New 5xx sequencing primer for B-globin
                     constructs.  Same position as JSL-70, but longer with a Tm
                     at 66C.  Max recorded sequencing read = 783 bases. 
                     Perfect match Tm = 65.5 C, although only 17 nt are
                     required to match. The actual sequence of the primer is
                     GGCCCTTTTG CTAATCATGT TCATACCTCT TATCT."
                     /label="HGTI-1732_Max=783"
     primer_bind     1224..1244
                     /Description="Jeng Shin designed sequencing primer @ 5xx
                     end of MDM2b polylinker facing towards gene.  xx723 is
                     longer, higher Tm versionxx.  Max recorded sequencing read
                     = 0 bases.  Perfect match Tm = 40.3 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is CATGTTCATA CCTCTTATCT T."
                     /label="HGTI-1278_Max=0"
     primer_bind     1226..1253
                     /Description="sFlt clone Add-musFlt-mIg: sequencing ORF 
                     Max recorded sequencing read = 0 bases.  Perfect match Tm
                     = 59.3 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is TGTTCATACC TCTTATCTTC
                     CTCCCACA."
                     /label="HGTI-2144_Max=0"
     sig_peptide     1323..1367
                     /created_by="alexbalazs"
                     /label="Signal Peptide"
     CDS             1323..5090
                     /locus_tag="nCoV S"
                     /modified_by="alexbalazs"
                     /label="SARS-CoV-2 S-gp41"
     mutation        2204
                     /Removed_BamHI_Site
                     /created_by="alexbalazs"
                     /label="T->C"
     misc_feature    3366..3377
                     /created_by="alexbalazs"
                     /modified_by="alexbalazs"
                     /label="S1/S2 Furin cleaveage site"
     misc_feature    3765..3770
                     /created_by="alexbalazs"
                     /label="S2' Cleavage site (between R and S)"
     misc_feature    4767..4778
                     /created_by="alexbalazs"
                     /label="ER Retention Signal"
     misc_feature    4947..4973
                     /created_by="alexbalazs"
                     /label="Aromatic Region"
     misc_feature    4962..5030
                     /created_by="alexbalazs"
                     /label="Transmembrane Region (TMHMM Predicted)"
     misc_feature    5061..5087
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     CDS             <5061..>5087
                     /codon_start=1
                     /locus_tag="nCoV_IDT_opt_spike"
                     /ApEinfo_label="nCoV_IDT_opt_spike"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="alexbalazs"
                     /label="nCoV_IDT_opt_spike"
     misc_feature    5085..>5087
                     /created_by="alexbalazs"
                     /label="ER Retention Signal"
     misc_feature    5136..5895
                     /Description="From 3rd exon of human beta globin, contains
                     polyA sequences commonly used in HGTI vector lab
                     constructs."
                     /label="beta_globin_pA"
     primer_bind     complement(5182..5199)
                     /Description="Jeng Shin designed sequencing primer @ 3xx
                     end of MDM2b polylinker facing towards gene.New
                     incarnation is 3149, reordered 1xx29xx01.  Max recorded
                     sequencing read =  bases.  Perfect match Tm = 54.2 C,
                     although only 17 nt are required to match.  The actual
                     sequence of the primer is gccagggcat tagccaca."
                     /label="HGTI-1279_Max="
     primer_bind     5305..5333
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     379 bases.  Perfect match Tm = 59.8 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is agcatctgga ttctgcctaa taaaaaaca."
                     /label="HGTI-1843_Max=379"
     polyA_site      5323..5347
                     /Description="Axxs added after these bases in globin
                     message."
                     /label="polyA_site"
     primer_bind     complement(5390..5414)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     692 bases.  Perfect match Tm = 58.1 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ttaaatgcac tgacctccca cattc."
                     /label="HGTI-1874_Max=692"
     primer_bind     5606..5639
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     643 bases.  Perfect match Tm = 59.1 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ttttacatta cttattgttt tagctgtcct catg."
                     /label="HGTI-1844_Max=643"
     primer_bind     complement(5685..5715)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     610 bases.  Perfect match Tm = 59.5 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is tatctctaag caagagaact gagtggagtc a."
                     /label="HGTI-1875_Max=610"
     misc_feature    5871..5895
                     /Description="End of globin sequences in typical pMD
                     constructs."
                     /label="End_of_globin"
     misc_feature    5909..5946
                     /Description="Extra EcoR I site of pMD-old-gag-pol.  This
                     sequence is definitely not in all MDM constructs. 
                     Probably only exists in a few older constructs prior to
                     the MDM2b set.  This sequence has been found to be
                     erroneous for some plasmids in our laboratory.  It would
                     be prudent to sequence the DNA to confirm the accuracy of
                     your records.  The sequence specified by this feature was
                     actually found to be:  ACATGGCAGT CTAGAATTCT TGAAGACGAA
                     AGGGCC."
                     /label="Correction-143"
     primer_bind     complement(5948..5978)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     711 bases.  Perfect match Tm = 58.3 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is gacattaacc tataaaaata ggcgtatcac g."
                     /label="HGTI-1856_Max=711"
     CDS             6135..6992
                     /Description="B-lactamase, confers ampicillin resistance,
                     no Pst I."
                     /label="bla"
     primer_bind     6169..6189
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     647 bases.  Perfect match Tm = 60.2 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ccttttttgc ggcattttgc c."
                     /label="HGTI-1886_Max=647"
     primer_bind     complement(6255..6278)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     661 bases.  Perfect match Tm = 58.0 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is atccagttcg atgtaaccca ctcg."
                     /label="HGTI-1855_Max=661"
     primer_bind     6469..6496
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     654 bases.  Perfect match Tm = 60.0 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is cggatggcat gacagtaaga gaattatg."
                     /label="HGTI-1885_Max=654"
     primer_bind     complement(6558..6578)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     650 bases.  Perfect match Tm = 58.4 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is agcggttagc tccttcggtc c."
                     /label="HGTI-1854_Max=650"
     primer_bind     6769..6793
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     593 bases.  Perfect match Tm = 59.6 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ataaagttgc aggaccactt ctgcg."
                     /label="HGTI-1884_Max=593"
     primer_bind     complement(6857..6878)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 0
                     bases.  Perfect match Tm = 61.0 C, although only 17 nt are
                     required to match.  The actual sequence of the primer is
                     ccccagtgct gcaatgatac cg."
                     /label="HGTI-1853_Max=0"
     primer_bind     7069..7097
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     564 bases.  Perfect match Tm = 58.2 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is tgaagatcct ttttgataat ctcatgacc."
                     /label="HGTI-1883_Max=564"
     primer_bind     complement(7149..7178)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     372 bases.  Perfect match Tm = 58.8 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is aaaaggatct caagaagatc ctttgatctt ."
                     /label="HGTI-1852_Max=372"
     misc_feature    7291..7334
                     /Description="Tag for High copy plasmid replication
                     xxpUC18xx"
                     /label="High_copy"
     primer_bind     7369..7396
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     565 bases.  Perfect match Tm = 60.5 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is catacctcgc tctgctaatc ctgttacc."
                     /label="HGTI-1882_Max=565"
     primer_bind     complement(7460..7478)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     601 bases.  Perfect match Tm = 60.1 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is cgaccgctgc gccttatcc."
                     /label="HGTI-1851_Max=601"
     primer_bind     7669..7694
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     511 bases.  Perfect match Tm = 59 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is atctttatag tcctgtcggg tttcgc."
                     /label="HGTI-1881_Max=511"
     misc_feature    7755..7780
                     /Description="From sequencing HDM-SNVgpm8.  May be present
                     in all HDM or even MDM plasmids.  No apparent functional
                     ramifications.  This sequence has been found to be
                     erroneous for some plasmids in our laboratory.  It would
                     be prudent to sequence the DNA to confirm the accuracy of
                     your records.  The sequence specified by this feature was
                     actually found to be:  AACGCCAGCA ACGGAGATGC GCCGCGTG."
                     /label="Correction-133"
     misc_feature    8022..8049
                     /Description="From sequencing HDM-SNVgpm8.  May be present
                     in all HDM and MDM plasmids.  No apparent functional
                     ramifications.  This sequence has been found to be
                     erroneous for some plasmids in our laboratory.  It would
                     be prudent to sequence the DNA to confirm the accuracy of
                     your records.  The sequence specified by this feature was
                     actually found to be:  CGAGGCGGCA TAAATCGCCG TGACGATC."
                     /label="Correction-134"
     primer_bind     8175..8200
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     662 bases.  Perfect match Tm = 60.7 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is agcgagaaga atcataatgg ggaagg."
                     /label="HGTI-1828_Max=662"
     misc_feature    8222..8334
                     /Description="SV40 5172..41, contains origin of
                     replication, from pMD cassette."
                     /label="SV40_ori"
     primer_bind     complement(8247..8273)
                     /Description="Add-musFLT-mIg Plasmid record #912  Max
                     recorded sequencing read = 618 bases.  Perfect match Tm =
                     59.3 C, although only 17 nt are required to match. The
                     actual sequence of the primer is tccagaagta gtgaggaggc
                     ttttttg."
                     /label="HGTI-8_Max=618"
ORIGIN      
        1 agcttggccc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
       61 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgggaccga tccagcctcc cctcgaagct gatcctgaga acttcagggt
      781 gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa gttcatgtca
      841 taggaagggg agaagtaaca gggtacacat attgaccaaa tcagggtaat tttgcatttg
      901 taattttaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattccg cgggcggccg
     1321 ccatgttcgt gttcctggtg ctgctgcctc tggtgtccag ccagtgtgtg aacctgacca
     1381 ccagaacaca gctgcctcca gcctacacca acagctttac cagaggcgtg tactaccccg
     1441 acaaggtgtt cagatccagc gtgctgcact ctacccagga cctgttcctg cctttcttca
     1501 gcaacgtgac ctggttccac gccatccacg tgtccggcac caatggcacc aagagattcg
     1561 acaaccccgt gctgcccttc aacgacgggg tgtactttgc cagcaccgag aagtccaaca
     1621 tcatcagagg ctggatcttc ggcaccacac tggacagcaa gacccagagc ctgctgatcg
     1681 tgaacaacgc caccaacgtg gtcatcaaag tgtgcgagtt ccagttctgc aacgacccct
     1741 tcctgggcgt ctactaccac aagaacaaca agagctggat ggaaagcgag ttccgggtgt
     1801 acagcagcgc caacaactgc accttcgagt acgtgtccca gcctttcctg atggacctgg
     1861 aaggcaagca gggcaacttc aagaacctgc gcgagttcgt gtttaagaac atcgacggct
     1921 acttcaaaat ctacagcaag cacaccccta tcaacctcgt gcgggatctg cctcagggct
     1981 tctctgctct ggaacccctg gtggatctgc ccatcggcat caacatcacc cggtttcaga
     2041 cactgctggc cctgcacaga agctacctga cacctggcga tagcagcagc ggatggacag
     2101 ctggtgccgc cgcttactat gtgggctacc tgcagcctag aaccttcctg ctgaagtaca
     2161 acgagaacgg caccatcacc gacgccgtgg attgtgctct ggaccctctg agcgagacaa
     2221 agtgcaccct gaagtccttc accgtggaaa agggcatcta ccagaccagc aacttccggg
     2281 tgcagcccac cgaatccatc gtgcggttcc ccaatatcac caatctgtgc cccttcggcg
     2341 aggtgttcaa tgccaccaga ttcgcctctg tgtacgcctg gaaccggaag cggatcagca
     2401 attgcgtggc cgactactcc gtgctgtaca actccgccag cttcagcacc ttcaagtgct
     2461 acggcgtgtc ccctaccaag ctgaacgacc tgtgcttcac aaacgtgtac gccgacagct
     2521 tcgtgatccg gggagatgaa gtgcggcaga ttgcccctgg acagacaggc aagatcgccg
     2581 actacaacta caagctgccc gacgacttca ccggctgtgt gattgcctgg aacagcaaca
     2641 acctggactc caaagtcggc ggcaactaca attacctgta ccggctgttc cggaagtcca
     2701 atctgaagcc cttcgagcgg gacatcagca ccgaaatcta tcaggccggc agcacccctt
     2761 gcaacggcgt ggaaggcttc aactgctact tcccactgca gtcctacggc tttcagccca
     2821 caaatggcgt gggctatcag ccctacagag tggtggtgct gagcttcgaa ctgctgcatg
     2881 cccctgccac agtgtgcggc cctaagaaaa gcaccaatct cgtgaagaac aaatgcgtga
     2941 acttcaactt caacggcctg accggcaccg gcgtgctgac agagagcaac aagaagttcc
     3001 tgccattcca gcagtttggc cgggatatcg ccgataccac agacgccgtt agagatcccc
     3061 agacactgga aatcctggac atcaccccat gcagcttcgg cggagtgtct gtgatcaccc
     3121 ctggcaccaa caccagcaat caggtggcag tgctgtacca ggacgtgaac tgtaccgaag
     3181 tgcccgtggc cattcacgcc gatcagctga cacctacatg gcgggtgtac tccaccggca
     3241 gcaatgtgtt tcagaccaga gccggctgtc tgatcggagc cgagcacgtg aacaatagct
     3301 acgagtgcga catccccatc ggcgctggca tctgtgccag ctaccagaca cagacaaaca
     3361 gccccagacg ggctagaagc gtggccagcc agagcatcat tgcctacaca atgtctctgg
     3421 gcgccgagaa cagcgtggcc tactccaaca actctatcgc tatccccacc aacttcacca
     3481 tcagcgtgac caccgagatc ctgcctgtgt ccatgaccaa gaccagcgtg gactgcacca
     3541 tgtacatctg cggcgattcc accgagtgct ccaacctgct gctgcagtac ggcagcttct
     3601 gcacccagct gaatagagcc ctgacaggga tcgccgtgga acaggacaag aacacccaag
     3661 aggtgttcgc ccaagtgaag caaatctaca agacccctcc tatcaaggac ttcggcggct
     3721 tcaatttcag ccagattctg cccgatccta gcaagcccag caagcggagc ttcatcgagg
     3781 acctgctgtt caacaaagtg acactggccg acgccggctt catcaagcag tatggcgatt
     3841 gtctgggcga cattgccgcc agggatctga tttgcgccca gaagtttaac ggactgacag
     3901 tgctgcctcc tctgctgacc gatgagatga tcgcccagta cacatctgcc ctgctggccg
     3961 gcacaatcac aagcggctgg acatttggag ctggcgccgc tctgcagatc ccctttgcta
     4021 tgcagatggc ctaccggttc aacggcatcg gagtgaccca gaatgtgctg tacgagaacc
     4081 agaagctgat cgccaaccag ttcaacagcg ccatcggcaa gatccaggac agcctgagca
     4141 gcacagcaag cgccctggga aagctgcagg acgtggtcaa ccagaatgcc caggcactga
     4201 acaccctggt caagcagctg tcctccaact tcggcgccat cagctctgtg ctgaacgata
     4261 tcctgagcag actggacaag gtggaagccg aggtgcagat cgacagactg atcaccggaa
     4321 ggctgcagtc cctgcagacc tacgttaccc agcagctgat cagagccgcc gagattagag
     4381 cctctgccaa tctggccgcc accaagatgt ctgagtgtgt gctgggccag agcaagagag
     4441 tggacttttg cggcaagggc taccacctga tgagcttccc tcagtctgcc cctcacggcg
     4501 tggtgtttct gcacgtgaca tacgtgcccg ctcaagagaa gaatttcacc accgctccag
     4561 ccatctgcca cgacggcaaa gcccactttc ctagagaagg cgtgttcgtg tccaacggca
     4621 cccattggtt cgtgacccag cggaacttct acgagcccca gatcatcacc accgacaaca
     4681 ccttcgtgtc tggcaactgc gacgtcgtga tcggcattgt gaacaatacc gtgtacgacc
     4741 ctctgcagcc cgagctggac agcttcaaag aggaactgga taagtacttt aagaaccaca
     4801 caagccccga cgtggacctg ggcgatatca gcggaatcaa tgccagcgtc gtgaacatcc
     4861 agaaagagat cgaccggctg aacgaggtgg ccaagaatct gaacgagagc ctgatcgacc
     4921 tgcaagaact ggggaagtac gagcagtaca tcaagtggcc ctggtacatc tggctgggct
     4981 ttatcgccgg actgattgcc atcgtgatgg tcacaatcat gctgtgttgc atgaccagct
     5041 gctgtagctg cctgaagggc tgttgctctt gcggctcttg ctgcaaataa aggatccaag
     5101 cttatcgata ccgtcgacct cgagggccca gatctaattc accccaccag tgcaggctgc
     5161 ctatcagaaa gtggtggctg gtgtggctaa tgccctggcc cacaagtatc actaagctcg
     5221 ctttcttgct gtccaatttc tattaaaggt tcctttgttc cctaagtcca actactaaac
     5281 tgggggatat tatgaagggc cttgagcatc tggattctgc ctaataaaaa acatttattt
     5341 tcattgcaat gatgtattta aattatttct gaatatttta ctaaaaaggg aatgtgggag
     5401 gtcagtgcat ttaaaacata aagaaatgaa gagctagttc aaaccttggg aaaatacact
     5461 atatcttaaa ctccatgaaa gaaggtgagg ctgcaaacag ctaatgcaca ttggcaacag
     5521 cccctgatgc ctatgcctta ttcatccctc agaaaaggat tcaagtagag gcttgatttg
     5581 gaggttaaag ttttgctatg ctgtatttta cattacttat tgttttagct gtcctcatga
     5641 atgtcttttc actacccatt tgcttatcct gcatctctca gccttgactc cactcagttc
     5701 tcttgcttag agataccacc tttcccctga agtgttcctt ccatgtttta cggcgagatg
     5761 gtttctcctc gcctggccac tcagccttag ttgtctctgt tgtcttatag aggtctactt
     5821 gaagaaggaa aaacaggggg catggtttga ctgtcctgtg agcccttctt ccctgcctcc
     5881 cccactcaca gtgacccgga atccctcgac atggcagtct agatcattct tgaagacgaa
     5941 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6001 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6061 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6121 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6181 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6241 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6301 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6361 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6421 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6481 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6541 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6601 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6661 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6721 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6781 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6841 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6901 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6961 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7021 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7081 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7141 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7201 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7261 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7321 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7381 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7441 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7501 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7561 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7621 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7681 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7741 ggagcctatg gaaaaacgcc agcaacggat gcgccgcgtg cggctgctgg agatggcgga
     7801 cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg
     7861 attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag
     7921 gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg
     7981 gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcccc
     8041 gtgacgatca gcggtccaat gatcgaagtt aggctggtaa gagccgcgag cgatccttga
     8101 agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc
     8161 ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcggg
     8221 gagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     8281 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catg
//
