LOCUS       2868_-_pTwist-WIV1-CoV_Spike-deltaC18       10411 bp    DNA     circular     13-JUL-2020
DEFINITION  WIV1-CoV in pTwist CMV BetaGlobin WPRE Neo.
ACCESSION   urn.local.alexbalazs.4f-c1mkgus
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Resistance markers: Ampicilin                                      
                                                                               
                                         This file was created using tools
            provided by Twist Bioscience.
FEATURES             Location/Qualifiers
     CDS             2..3718
                     /created_by="alexbalazs"
                     /label="WIV1-CoV Spike"
     misc_feature    3715^3716
                     /Original_Bases="TTCGATGAGGATGATTCAGAACCCGTACTCAAGGGTGTGAA
                     ACTCCACTATACC"
                     /label="TTCGATGAGGATGATTCAGAACCCGTACTCAAGGGTGTGAAACTCCACTA
                     TACC"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    3789..4377
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /X-Transferred_From="229E-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; Avian-Inf in pTwist CMV BetaGlobin WPRE Neo;
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo; Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo; HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo; MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; NL63-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="229E-CoV in pTwist CMV BetaGlobin WPRE
                     Neo, Avian-Inf in pTwist CMV BetaGlobin WPRE Neo,
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo, Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo, HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo, MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo, NL63-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo, SARS-CoV-2 in
                     pTwist CMV BetaGlobin WPRE Neo,
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /Transferred_Similarity="100.00%"
                     /label="WPRE"
     misc_feature    4388..4398
                     /X-Transferred_From="229E-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; Avian-Inf in pTwist CMV BetaGlobin WPRE Neo;
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo; Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo; HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo; MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; NL63-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="229E-CoV in pTwist CMV BetaGlobin WPRE
                     Neo, Avian-Inf in pTwist CMV BetaGlobin WPRE Neo,
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo, Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo, HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo, MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo, NL63-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo, SARS-CoV-2 in
                     pTwist CMV BetaGlobin WPRE Neo,
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /Transferred_Similarity="100.00%"
                     /label="Stop codons x 3"
     misc_feature_   4414..4646
                     /label="SV40_PA"
     polyA_site      4421..4612
                     /X-Transferred_From="229E-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; Avian-Inf in pTwist CMV BetaGlobin WPRE Neo;
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo; Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo; HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo; MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; NL63-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="229E-CoV in pTwist CMV BetaGlobin WPRE
                     Neo, Avian-Inf in pTwist CMV BetaGlobin WPRE Neo,
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo, Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo, HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo, MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo, NL63-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo, SARS-CoV-2 in
                     pTwist CMV BetaGlobin WPRE Neo,
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /Transferred_Similarity="100.00%"
                     /label="SV40 PolyA"
     terminator      4666..4713
                     /X-Transferred_From="229E-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; Avian-Inf in pTwist CMV BetaGlobin WPRE Neo;
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo; Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo; HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo; MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; NL63-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="229E-CoV in pTwist CMV BetaGlobin WPRE
                     Neo, Avian-Inf in pTwist CMV BetaGlobin WPRE Neo,
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo, Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo, HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo, MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo, NL63-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo, SARS-CoV-2 in
                     pTwist CMV BetaGlobin WPRE Neo,
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /Transferred_Similarity="100.00%"
                     /label="T7 Terminator"
     terminator      4743..4879
                     /X-Transferred_From="229E-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; Avian-Inf in pTwist CMV BetaGlobin WPRE Neo;
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo; Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo; HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo; MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; NL63-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="229E-CoV in pTwist CMV BetaGlobin WPRE
                     Neo, Avian-Inf in pTwist CMV BetaGlobin WPRE Neo,
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo, Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo, HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo, MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo, NL63-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo, SARS-CoV-2 in
                     pTwist CMV BetaGlobin WPRE Neo,
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /Transferred_Similarity="100.00%"
                     /label="RrnG Bacterial Terminator"
     misc_feature    4923..4994
                     /X-Transferred_From="229E-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; Avian-Inf in pTwist CMV BetaGlobin WPRE Neo;
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo; Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo; HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo; MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; NL63-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="229E-CoV in pTwist CMV BetaGlobin WPRE
                     Neo, Avian-Inf in pTwist CMV BetaGlobin WPRE Neo,
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo, Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo, HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo, MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo, NL63-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo, SARS-CoV-2 in
                     pTwist CMV BetaGlobin WPRE Neo,
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /Transferred_Similarity="100.00%"
                     /label="3'HS1 BetaGlobin Insulator"
     promoter        5033..5232
                     /note="SV40"
                     /X-Transferred_From="229E-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; Avian-Inf in pTwist CMV BetaGlobin WPRE Neo;
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo; Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo; HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo; MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; NL63-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="229E-CoV in pTwist CMV BetaGlobin WPRE
                     Neo, Avian-Inf in pTwist CMV BetaGlobin WPRE Neo,
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo, Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo, HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo, MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo, NL63-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo, SARS-CoV-2 in
                     pTwist CMV BetaGlobin WPRE Neo,
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /Transferred_Similarity="100.00%"
                     /label="SV40"
     CDS             5249..6043
                     /X-Transferred_From="229E-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; Avian-Inf in pTwist CMV BetaGlobin WPRE Neo;
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo; Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo; HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo; MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; NL63-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="229E-CoV in pTwist CMV BetaGlobin WPRE
                     Neo, Avian-Inf in pTwist CMV BetaGlobin WPRE Neo,
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo, Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo, HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo, MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo, NL63-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo, SARS-CoV-2 in
                     pTwist CMV BetaGlobin WPRE Neo,
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /Transferred_Similarity="100.00%"
                     /label="NeoG418"
     polyA_site      6054..6278
                     /X-Transferred_From="229E-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; Avian-Inf in pTwist CMV BetaGlobin WPRE Neo;
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo; Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo; HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo; MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; NL63-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="229E-CoV in pTwist CMV BetaGlobin WPRE
                     Neo, Avian-Inf in pTwist CMV BetaGlobin WPRE Neo,
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo, Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo, HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo, MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo, NL63-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo, SARS-CoV-2 in
                     pTwist CMV BetaGlobin WPRE Neo,
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /Transferred_Similarity="100.00%"
                     /label="BgH PolyA"
     rep_origin      6414..7298
                     /note="pMB1 (Bacterial, 500-800 copies)"
                     /X-Transferred_From="229E-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; Avian-Inf in pTwist CMV BetaGlobin WPRE Neo;
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo; Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo; HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo; MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; NL63-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="229E-CoV in pTwist CMV BetaGlobin WPRE
                     Neo, Avian-Inf in pTwist CMV BetaGlobin WPRE Neo,
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo, Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo, HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo, MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo, NL63-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo, SARS-CoV-2 in
                     pTwist CMV BetaGlobin WPRE Neo,
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /Transferred_Similarity="100.00%"
                     /label="pMB1 (Bacterial, 500-800 copies)"
     CDS             7808..8668
                     /X-Transferred_From="229E-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; Avian-Inf in pTwist CMV BetaGlobin WPRE Neo;
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo; Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo; HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo; MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; NL63-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="229E-CoV in pTwist CMV BetaGlobin WPRE
                     Neo, Avian-Inf in pTwist CMV BetaGlobin WPRE Neo,
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo, Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo, HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo, MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo, NL63-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo, SARS-CoV-2 in
                     pTwist CMV BetaGlobin WPRE Neo,
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /Transferred_Similarity="100.00%"
                     /label="Ampicillin (AmpR)"
     terminator      8682..8818
                     /note="RrnG Bacterial Terminator"
                     /X-Transferred_From="229E-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; Avian-Inf in pTwist CMV BetaGlobin WPRE Neo;
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo; Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo; HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo; MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; NL63-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="229E-CoV in pTwist CMV BetaGlobin WPRE
                     Neo, Avian-Inf in pTwist CMV BetaGlobin WPRE Neo,
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo, Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo, HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo, MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo, NL63-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo, SARS-CoV-2 in
                     pTwist CMV BetaGlobin WPRE Neo,
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /Transferred_Similarity="100.00%"
                     /label="rrnG terminator"
     misc_feature    8822..8828
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    8969..9040
                     /X-Transferred_From="229E-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; Avian-Inf in pTwist CMV BetaGlobin WPRE Neo;
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo; Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo; HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo; MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; NL63-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="229E-CoV in pTwist CMV BetaGlobin WPRE
                     Neo, Avian-Inf in pTwist CMV BetaGlobin WPRE Neo,
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo, Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo, HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo, MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo, NL63-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo, SARS-CoV-2 in
                     pTwist CMV BetaGlobin WPRE Neo,
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /Transferred_Similarity="100.00%"
                     /label="5'HS1 BetaGlobin Insulator"
     misc_feature_   9044..9086
                     /label="T7"
     promoter        9189..9759
                     /note="CMV Promoter"
                     /X-Transferred_From="229E-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; Avian-Inf in pTwist CMV BetaGlobin WPRE Neo;
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo; Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo; HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo; MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; NL63-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="229E-CoV in pTwist CMV BetaGlobin WPRE
                     Neo, Avian-Inf in pTwist CMV BetaGlobin WPRE Neo,
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo, Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo, HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo, MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo, NL63-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo, SARS-CoV-2 in
                     pTwist CMV BetaGlobin WPRE Neo,
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /Transferred_Similarity="100.00%"
     misc_feature_   9709..9827
                     /label="CMV2"
     misc_feature    9760..10403
                     /X-Transferred_From="229E-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; Avian-Inf in pTwist CMV BetaGlobin WPRE Neo;
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo; Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo; HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo; MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo; NL63-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo;
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="229E-CoV in pTwist CMV BetaGlobin WPRE
                     Neo, Avian-Inf in pTwist CMV BetaGlobin WPRE Neo,
                     BetaEngland1-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     Bovine-CoV in pTwist CMV BetaGlobin WPRE Neo, Camel-CoV in
                     pTwist CMV BetaGlobin WPRE Neo, HKU1-CoV in pTwist CMV
                     BetaGlobin WPRE Neo, MERS-CoV in pTwist CMV BetaGlobin
                     WPRE Neo, NL63-CoV in pTwist CMV BetaGlobin WPRE Neo,
                     OC43-CoV in pTwist CMV BetaGlobin WPRE Neo, SARS-CoV-2 in
                     pTwist CMV BetaGlobin WPRE Neo,
                     pTwist-CMV-BetaGlobin-WPRE-Neo"
                     /Transferred_Similarity="100.00%"
                     /label="BGI"
ORIGIN      
        1 catgaaattg ctggtactgg tctttgcgac actcgtgtca agctatacca tcgaaaagtg
       61 tcttgacttc gatgatcgga cacccccggc caatacccag ttcctgtcaa gccaccgagg
      121 ggtctattac ccggacgata ttttcagaag caatgtactg catttggtac aggaccactt
      181 tctccctttt gattccaatg ttactaggtt tatcactttt gggcttaatt tcgacaatcc
      241 tataattcct ttcaaagatg gcatctactt cgctgctact gaaaagtcca acgttattag
      301 aggctgggtg tttggatcaa caatgaataa caagtctcaa tccgtcatca taatgaacaa
      361 tagtaccaac cttgtaatcc gggcttgcaa ttttgaattg tgcgacaatc ctttctttgt
      421 tgtgctcaag agcaacaata cacaaattcc tagctacatc tttaataatg ccttcaactg
      481 tactttcgag tatgtgagca aagactttaa tcttgacctc ggcgagaagc ccggaaactt
      541 caaagatttg cgcgaatttg tgtttagaaa caaagacggc ttcctgcacg tctactccgg
      601 ctatcaacca atcagcgcgg ccagcgggtt gccaactggc tttaatgcgc tcaagcctat
      661 tttcaagctc ccgctgggaa tcaacattac taatttccgc accctgctca ccgcttttcc
      721 ccctcgccct gactattggg gcacaagcgc cgcagcttat tttgtcggtt acttgaaacc
      781 cactaccttc atgttgaagt acgacgaaaa cggcactatt acagatgcag tagactgtag
      841 ccaaaatcca ctggcggagc tgaaatgcag tgtcaagtca ttcgagatcg acaaaggcat
      901 ctaccagact tcaaattttc gcgtcgcacc atccaaagaa gtagtgcgct ttccaaatat
      961 caccaatctg tgtccttttg gtgaagtgtt taacgccacc acattcccca gtgtttacgc
     1021 ctgggagaga aagaggatca gcaactgtgt ggctgactat tcagtgctgt ataatagcac
     1081 ctcttttagt acattcaaat gttacggggt gtcagccacc aagctcaacg acctgtgttt
     1141 tagcaacgta tacgcagata gtttcgtcgt caaaggagat gacgttagac agatcgctcc
     1201 cggccaaaca ggggttattg ctgactacaa ctataagctg cccgatgatt ttactggctg
     1261 tgtgctggct tggaacacac gcaacataga cgccacgcag acgggcaatt acaactacaa
     1321 gtaccgttcc ctgagacacg gcaaactgag accctttgaa agggacatct ccaatgtgcc
     1381 tttttctcct gacggcaagc cctgtacccc acccgctttt aattgttact ggccgctgaa
     1441 tgattatggt ttctatataa ctaacggcat aggctatcag ccataccggg ttgttgtgct
     1501 cagtttcgag ttgcttaacg ctccagcaac tgtctgtggc ccaaaactca gtaccgattt
     1561 gataaagaat caatgcgtga actttaattt taacggattg acagggacag gcgttttgac
     1621 tcctagttcc aaacggttcc aaccctttca acaattcggg cgcgatgtct ctgatttcac
     1681 cgattctgta agagatccta agacctctga aatacttgac atcagtcctt gttctttcgg
     1741 cggtgtatct gtaatcacac cgggcactaa cacgagcagt gaagtagccg tactgtatca
     1801 ggatgttaat tgcactgacg tcccggtagc aatacacgca gatcaactga cgccatcttg
     1861 gcgagttcac tctaccggca ataacgtctt tcaaactcaa gcaggatgct tgattggggc
     1921 ggagcacgta gataccagtt atgagtgcga tattccgata ggcgcgggca tctgtgccag
     1981 ttatcacacc gtcagtagtc tccggtctac tagccagaaa agtattgtcg catacacaat
     2041 gtcactcggt gccgatagtt ctatcgccta ctccaacaac accattgcaa tccccactaa
     2101 tttctccatc tccatcacca ccgaagtgat gcctgtcagc atggctaaaa ccagcgttga
     2161 ctgcaatatg tatatttgcg gcgattcaac ggagtgcgcg aatttgctcc ttcaatatgg
     2221 ctcattttgc acacaactga accgggctct ctccggaatc gcggtggaac aagacaggaa
     2281 cactcgtgaa gtgttcgcgc aagtcaagca aatgtataag actccgaccc ttaaagattt
     2341 cggcgggttt aatttctccc aaattctccc ggaccccttg aagcctacca agcgcagctt
     2401 tatcgaagat ttgttgttca acaaagttac ccttgccgac gccggtttta tgaagcaata
     2461 cggggaatgt cttggagata taaatgcgcg tgatcttatt tgtgcccaaa aattcaacgg
     2521 cttgacagtg ctgcccccac tcctgacgga cgatatgata gccgcataca ctgcggcgtt
     2581 ggtgagcggc accgccaccg ctggctggac tttcggggca ggagccgctt tgcaaattcc
     2641 ctttgcgatg caaatggcgt ataggtttaa cgggataggc gtaacacaaa atgttctcta
     2701 cgaaaatcaa aagcaaatcg ctaatcaatt taacaaggcc atttcccaaa ttcaggagag
     2761 tctcacaaca acatctactg ctctgggaaa actgcaagac gttgtgaatc aaaacgctca
     2821 agcactcaat acactcgtta agcaactgtc ctctaacttc ggggccatat cttctgtgct
     2881 gaatgatatt ctgtcacgcc tcgataaagt tgaggctgaa gtccagatag acagactgat
     2941 tacgggcagg ctgcaaagcc tgcaaaccta tgtgacgcag cagctcattc gagctgctga
     3001 aattcgcgct tcagcgaacc tcgcagccac caaaatgtct gaatgtgttt tggggcaaag
     3061 caaacgggtc gatttctgtg gtaaaggata ccatttgatg tcttttcccc aagcagctcc
     3121 acacggcgtt gtctttctgc atgtgaccta tgtccccagc caagaacgga atttcacaac
     3181 ggctcccgcc atctgtcacg aagggaaggc gtattttcct cgggaagggg tgtttgtttt
     3241 caatgggacc tcatggttta ttacgcagcg caatttcttt agcccccaaa taataaccac
     3301 agacaataca tttgtcagtg gtagttgcga tgtagtcatt ggaataatca ataatacggt
     3361 gtacgatcct ttgcagccag aattggatag cttcaaagaa gaacttgaca aatactttaa
     3421 gaaccacaca agtccagacg tggacttggg agatataagc gggataaacg cgagcgttgt
     3481 caatattcaa aaggaaatcg acaggctgaa tgaagtagct aagaatctga atgaatccct
     3541 cattgatttg caagaactcg ggaagtatga gcaatatatc aagtggccgt ggtatgtttg
     3601 gctgggtttt atagccggtc tgattgcaat cgtcatggtg acaattctgt tgtgttgcat
     3661 gacttcatgc tgtagttgcc tcaaaggcgc atgtagctgt ggcagttgtt gtaaataaag
     3721 gatcctctag aagttgtctc ctcctgcact gactgactga tacaatcgat ttctggatcc
     3781 gcaggcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     3841 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     3901 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     3961 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4021 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4081 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4141 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     4201 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4261 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4321 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     4381 tctagcttga ctgactgaga tacagcgtac cttcagctca cagacatgat aagatacatt
     4441 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     4501 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     4561 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     4621 taaaacctct acaaatgtgg tattggccca tctctatcgg tatcgtagca taaccccttg
     4681 gggcctctaa acgggtcttg aggggttttt tgtgcccctc gggccggatt gctatctacc
     4741 ggcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact cccacatatg
     4801 ccagattcag caacggatac ggcttcccca acttgcccac ttccatacgt gtcctcctta
     4861 ccagaaattt atccttaagg tcgtcagcta tcctgcaggc gatctctcga tttcgatcaa
     4921 gacattcctt taatggtctt ttctggacac cactaggggt cagaagtagt tcatcaaact
     4981 ttcttccctc cctaatctca ttggttacct tgggctatcg aaacttaatt aagcgatctg
     5041 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     5101 ccgcccagtt ccgcccattc tccgccccat cgctgactaa ttttttttat ttatgcagag
     5161 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5221 ctaggctttt gcaaaggagg tagccaacat gattgaacaa gatggattgc acgcaggttc
     5281 tcccgccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5341 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5401 cgacctgtcc ggtgccctga atgaactcca ggacgaggca gcgcggctat cgtggctggc
     5461 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     5521 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     5581 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     5641 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     5701 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     5761 cgccaggctc aaggcgcgga tgcccgacgg cgaggatctc gtcgtgaccc acggcgatgc
     5821 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     5881 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     5941 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6001 gcagcgcatc gccttctatc gccttcttga cgagttcttc tagtatgtaa gccctgtgcc
     6061 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     6121 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     6181 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     6241 caatagcagg catgctgggg atgcggtggg ctctatggtt aattaaccag tcaagtcagc
     6301 tacttggcga gatcgacttg tctgggtttc gactacgctc agaattgcgt cagtcaagtt
     6361 cgatctggtc cttgctattg cacccgttct ccgattacga gtttcattta aatcatgtga
     6421 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6481 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6541 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6601 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6661 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6721 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6781 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6841 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6901 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6961 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7021 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7081 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7141 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7201 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7261 atctcagcga tctgtctatt tcgttcatcc atagttgcat ttaaatttcc gaactctcca
     7321 aggccctcgt cggaaaatct tcaaaccttt cgtccgatcc atcttgcagg ctacctctcg
     7381 aacgaactat cgcaagtctc ttggccggcc ttgcgccttg gctattgctt ggcagcgcct
     7441 atcgccaggt attactccaa tcccgaatat ccgagatcgg gatcacccga gagaagttca
     7501 acctacatcc tcaatcccga tctatccgag atccgaggaa tatcgaaatc ggggcgcgcc
     7561 tggtgtaccg agaacgatcc tctcagtgcg agtctcgacg atccatatcg ttgcttggca
     7621 gtcagccagt cggaatccag cttgggaccc aggaagtcca atcgtcagat attgtactca
     7681 agcctggtca cggcagcgta ccgatctgtt taaacctaga tattgatagt ctgatcggtc
     7741 aacgtataat cgagtcctag cttttgcaaa catctatcaa gagacaggat cagcaggagg
     7801 ctttcgcatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7861 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7921 gggtgcgcga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7981 tcgccccgaa gaacgctttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8041 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8101 tgacttggtt gagtattcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8161 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8221 aacgattgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8281 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8341 cacgatgcct gtagcaatgg caacaacctt gcgtaaacta ttaactggcg aactacttac
     8401 tctagcttcc cggcaacagt tgatagactg gatggaggcg gataaagttg caggaccact
     8461 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8521 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8581 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8641 aggtgcctca ctgattaagc attggtaacc gattctaggt gcattggcgc agaaaaaaat
     8701 gcctgatgcg acgctgcgcg tcttatactc ccacatatgc cagattcagc aacggatacg
     8761 gcttccccaa cttgcccact tccatacgtg tcctccttac cagaaattta tccttaagat
     8821 cccgaatcgt ttaaactcga ctctggctct atcgaatctc cgtcgtttcg agcttacgcg
     8881 aacagccgtg gcgctcattt gctcgtcggg catcgaatct cgtcagctat cgtcagctta
     8941 cctttttggc agcgatcgcg gctcccgaca tcttggacca ttagctccac aggtatcttc
     9001 ttccctctag tggtcataac agcagcttca gctacctctc aattcaaaaa acccctcaag
     9061 acccgtttag aggccccaag gggttatgct atcaatcgtt gcgttacaca cacaaaaaac
     9121 caacacacat ccatcttcga tggatagcga ttttattatc taactgctga tcgagtgtag
     9181 ccagatctag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     9241 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     9301 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     9361 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     9421 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     9481 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     9541 gctgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     9601 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     9661 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     9721 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatcgcct ggagacgcca
     9781 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc cctcgaagct
     9841 tacatgtggt accgagctcg gatcctgaga acttcagggt gagtctatgg gacccttgat
     9901 gttttctttc cccttctttt ctatggttaa gttcatgtca taggaagggg agaagtaaca
     9961 gggtacacat attgaccaaa tcagggtaat tttgcatttg taattttaaa aaatgctttc
    10021 ttcttttaat atactttttt gtttatctta tttctaatac tttccctaat ctctttcttt
    10081 cagggcaata atgatacaat gtatcatgcc tctttgcacc attctaaaga ataacagtga
    10141 taatttctgg gttaaggcaa tagcaatatt tctgcatata aatatttctg catataaatt
    10201 gtaactgatg taagaggttt catattgcta atagcagcta caatccagct accattctgc
    10261 ttttatttta tggttgggat aaggctggat tattctgagt ccaagctagg cccttttgct
    10321 aatcatgttc atacctctta tcttcctccc acagctcctg ggcaacgtgc tggtctgtgt
    10381 gctggcccat cactttggca aaggcggccg c
//
