LOCUS       HDM-VSV-G               6237 bp    DNA     circular UNA 02-FEB-2021
DEFINITION  HDM VSVG as corrected by Addgene.
ACCESSION   urn.local.alexbalazs.16-cyun3zi
VERSION     urn.local.alexbalazs.16-cyun3zi
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    9..679
                     /Description="CMV promoter.  Transcription starts with
                     next base.  5 prime end represents complete extent of the
                     CMV promoter in MDM based plasmids, which corresponds to
                     positions 471..1141 of hCMVie genbank record accession
                     X03922."
                     /label="CMV"
                     /note="Geneious type: promoter prokaryotic"
     misc_feature    96..679
                     /Description="CMV promoter.  Transcription starts with
                     next base.  5 prime end starts at Spe I site only.  The
                     CMV region in this construct may extend further upstream."
                     /label="CMV"
                     /note="Geneious type: promoter prokaryotic"
     primer_bind     151..178
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     638 bases.  Perfect match Tm = 58.7 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is agttccgcgt tacataactt acggtaaa."
                     /label="HGTI-1832_Max=638"
     primer_bind     complement(236..260)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     672 bases.  Perfect match Tm = 59.1 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ccctattggc gttactatgg gaaca."
                     /label="HGTI-1863_Max=672"
     primer_bind     complement(536..560)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     485 bases.  Perfect match Tm = 58.0 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is aaacaaactc ccattgacgt caatg."
                     /label="HGTI-1864_Max=485"
     primer_bind     561..593
                     /Description="sequencing pHD from 3xx end  Max recorded
                     sequencing read = 707 bases. Perfect match Tm = 71.5 C,
                     although only 17 nt are required to match.  The actual
                     sequence of the primer is tggcaccaaa atcaacggga ctttccaaaa
                     tgt."
                     /label="HGTI-2021_Max=707"
     primer_bind     612..652
                     /Description="?  Max recorded sequencing read = 162 bases.
                      Perfect match Tm = 76.1 C, although only 17 nt are
                     required to match.  The actual sequence of the primer is
                     gcgcactagt catatggtag gcgtgtacgg tgggaggtct a."
                     /label="HGTI-1220_Max=162"
     primer_bind     617..638
                     /Description="Sequencing primers for pHJ1  Max recorded
                     sequencing read = 311 bases. Perfect match Tm = 62.5 C,
                     although only 17 nt are required to match.  The actual
                     sequence of the primer is GCAAATGGGC GGTAGGCGTG TA."
                     /label="HGTI-1204_Max=311"
     misc_feature    628..679
                     /Description="CMV TATA box sequences, from -52 to -1. 
                     Wild type, with Sac I site."
                     /label="CMV_TATA"
     primer_bind     complement(662..703)
                     /Description="linker from SacI of CMV bridging into BglII
                     of HIV R.  Max recorded sequencing read =  bases.  Perfect
                     match Tm = 74.8 C, although only 17 nt are required to
                     match.  The actual sequence of the primer is gatcTGGTCT
                     AACCAGAGAG ACCCcggttc actaaacgAG CT."
                     /label="HGTI-1192_Max="
     misc_feature    680..750
                     /Description="5xx untranslated region from CMV immediate
                     early promoter.  The 3 prime end corresponds to the full
                     extent of CMV sequences in the MDM constructs."
                     /label="CMV_5xxUTR"
     primer_bind     692..715
                     /Description="PCR or sequencing primer located in CMV
                     sequence just after 5xx end of RNA transcript.  Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     63.9 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GAGACGCCAT CCACGCTGTT
                     TTGA."
                     /label="HGTI-761_Max="
     primer_bind     751..775
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     595 bases.  Perfect match Tm = 58.7 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is cctcgaagct gatcctgaga acttc."
                     /label="HGTI-1834_Max=595"
     misc_feature    779..1254
                     /Description="Human B-globin 2nd intron, Rsa I deleted."
                     /label="B-globin_intron"
     primer_bind     complement(833..860)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     636 bases.  Perfect match Tm = 59.3 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is tgttacttct ccccttccta tgacatga."
                     /label="HGTI-1865_Max=636"
     primer_bind     complement(952..981)
                     /Description="sequencing primer in globin intron facing
                     left.  Max recorded sequencing read = 610 bases.  Perfect
                     match Tm = 53.9 C, although only 17 nt are required to
                     match.  The actual sequence of the primer is gaaagaaaga
                     gattagggaa agtattagaa ."
                     /label="HGTI-2022_Max=610"
     primer_bind     1051..1082
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     639 bases.  Perfect match Tm = 58.2 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is gttaaggcaa tagcaatatt tctgcatata aa."
                     /label="HGTI-1835_Max=639"
     primer_bind     complement(1135..1160)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     639 bases.  Perfect match Tm = 59.3 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is gcagaatggt agctggattg tagctg."
                     /label="HGTI-1866_Max=639"
     primer_bind     1209..1243
                     /Description="New 5xx sequencing primer for B-globin
                     constructs.  Same position as JSL-70, but longer with a Tm
                     at 66C.  Max recorded sequencing read = 783 bases. 
                     Perfect match Tm = 65.5 C, although only 17 nt are
                     required to match. The actual sequence of the primer is
                     GGCCCTTTTG CTAATCATGT TCATACCTCT TATCT."
                     /label="HGTI-1732_Max=783"
     primer_bind     1224..1244
                     /Description="Jeng Shin designed sequencing primer @ 5xx
                     end of MDM2b polylinker facing towards gene.  xx723 is
                     longer, higher Tm versionxx.  Max recorded sequencing read
                     = 0 bases.  Perfect match Tm = 40.3 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is CATGTTCATA CCTCTTATCT T."
                     /label="HGTI-1278_Max=0"
     primer_bind     1226..1253
                     /Description="sFlt clone Add-musFlt-mIg: sequencing ORF 
                     Max recorded sequencing read = 0 bases.  Perfect match Tm
                     = 59.3 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is TGTTCATACC TCTTATCTTC
                     CTCCCACA."
                     /label="HGTI-2144_Max=0"
     misc_feature    1309..1315
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     CDS             1316..2851
                     /label="VSV-G"
     primer_bind     complement(1398..1425)
                     /label="HGTI-2045_Max="
     mutation        1484
                     /label="A->T"
     mutation        1603
                     /label="G->T"
     primer_bind     complement(1651..1675)
                     /label="HGTI-2046_Max="
     primer_bind     2066..2090
                     /label="HGTI-2042_Max="
     primer_bind     complement(2147..2175)
                     /label="HGTI-2048_Max="
     primer_bind     2316..2347
                     /label="HGTI-2043_Max="
     primer_bind     complement(2398..2425)
                     /label="HGTI-2049_Max="
     primer_bind     complement(2650..2675)
                     /label="HGTI-2050_Max="
     misc_feature    2852..2969
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    2852..2969
                     /created_by="alexbalazs"
                     /label="Additional non-coding VSV sequence"
     misc_feature    3039..3798
                     /Description="From 3rd exon of human beta globin, contains
                     polyA sequences commonly used in HGTI vector lab
                     constructs."
                     /label="beta_globin_pA"
     primer_bind     complement(3085..3102)
                     /Description="Jeng Shin designed sequencing primer @ 3xx
                     end of MDM2b polylinker facing towards gene.New
                     incarnation is 3149, reordered 1xx29xx01.  Max recorded
                     sequencing read =  bases.  Perfect match Tm = 54.2 C,
                     although only 17 nt are required to match.  The actual
                     sequence of the primer is gccagggcat tagccaca."
                     /label="HGTI-1279_Max="
     primer_bind     3208..3236
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     379 bases.  Perfect match Tm = 59.8 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is agcatctgga ttctgcctaa taaaaaaca."
                     /label="HGTI-1843_Max=379"
     polyA_site      3226..3250
                     /Description="Axxs added after these bases in globin
                     message."
                     /label="polyA_site"
     primer_bind     complement(3293..3317)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     692 bases.  Perfect match Tm = 58.1 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ttaaatgcac tgacctccca cattc."
                     /label="HGTI-1874_Max=692"
     primer_bind     3509..3542
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     643 bases.  Perfect match Tm = 59.1 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ttttacatta cttattgttt tagctgtcct catg."
                     /label="HGTI-1844_Max=643"
     primer_bind     complement(3588..3618)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     610 bases.  Perfect match Tm = 59.5 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is tatctctaag caagagaact gagtggagtc a."
                     /label="HGTI-1875_Max=610"
     misc_feature    3774..3798
                     /Description="End of globin sequences in typical pMD
                     constructs."
                     /label="End_of_globin"
     misc_feature    3812..3849
                     /Description="Extra EcoR I site of pMD-old-gag-pol.  This
                     sequence is definitely not in all MDM constructs. 
                     Probably only exists in a few older constructs prior to
                     the MDM2b set.  This sequence has been found to be
                     erroneous for some plasmids in our laboratory.  It would
                     be prudent to sequence the DNA to confirm the accuracy of
                     your records.  The sequence specified by this feature was
                     actually found to be:  ACATGGCAGT CTAGAATTCT TGAAGACGAA
                     AGGGCC."
                     /label="Correction-143"
     primer_bind     complement(3851..3881)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     711 bases.  Perfect match Tm = 58.3 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is gacattaacc tataaaaata ggcgtatcac g."
                     /label="HGTI-1856_Max=711"
     CDS             4038..4895
                     /Description="B-lactamase, confers ampicillin resistance,
                     no Pst I."
                     /label="bla"
     primer_bind     4072..4092
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     647 bases.  Perfect match Tm = 60.2 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ccttttttgc ggcattttgc c."
                     /label="HGTI-1886_Max=647"
     primer_bind     complement(4158..4181)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     661 bases.  Perfect match Tm = 58.0 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is atccagttcg atgtaaccca ctcg."
                     /label="HGTI-1855_Max=661"
     primer_bind     4372..4399
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     654 bases.  Perfect match Tm = 60.0 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is cggatggcat gacagtaaga gaattatg."
                     /label="HGTI-1885_Max=654"
     primer_bind     complement(4461..4481)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     650 bases.  Perfect match Tm = 58.4 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is agcggttagc tccttcggtc c."
                     /label="HGTI-1854_Max=650"
     primer_bind     4672..4696
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     593 bases.  Perfect match Tm = 59.6 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ataaagttgc aggaccactt ctgcg."
                     /label="HGTI-1884_Max=593"
     primer_bind     complement(4760..4781)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 0
                     bases.  Perfect match Tm = 61.0 C, although only 17 nt are
                     required to match.  The actual sequence of the primer is
                     ccccagtgct gcaatgatac cg."
                     /label="HGTI-1853_Max=0"
     primer_bind     4972..5000
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     564 bases.  Perfect match Tm = 58.2 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is tgaagatcct ttttgataat ctcatgacc."
                     /label="HGTI-1883_Max=564"
     primer_bind     complement(5052..5081)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     372 bases.  Perfect match Tm = 58.8 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is aaaaggatct caagaagatc ctttgatctt ."
                     /label="HGTI-1852_Max=372"
     misc_feature    5194..5237
                     /Description="Tag for High copy plasmid replication
                     xxpUC18xx"
                     /label="High_copy"
     primer_bind     5272..5299
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     565 bases.  Perfect match Tm = 60.5 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is catacctcgc tctgctaatc ctgttacc."
                     /label="HGTI-1882_Max=565"
     primer_bind     complement(5363..5381)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     601 bases.  Perfect match Tm = 60.1 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is cgaccgctgc gccttatcc."
                     /label="HGTI-1851_Max=601"
     primer_bind     5572..5597
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     511 bases.  Perfect match Tm = 59 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is atctttatag tcctgtcggg tttcgc."
                     /label="HGTI-1881_Max=511"
     misc_feature    5658..5683
                     /Description="From sequencing HDM-SNVgpm8.  May be present
                     in all HDM or even MDM plasmids.  No apparent functional
                     ramifications.  This sequence has been found to be
                     erroneous for some plasmids in our laboratory.  It would
                     be prudent to sequence the DNA to confirm the accuracy of
                     your records.  The sequence specified by this feature was
                     actually found to be:  AACGCCAGCA ACGGAGATGC GCCGCGTG."
                     /label="Correction-133"
     misc_feature    5925..5952
                     /Description="From sequencing HDM-SNVgpm8.  May be present
                     in all HDM and MDM plasmids.  No apparent functional
                     ramifications.  This sequence has been found to be
                     erroneous for some plasmids in our laboratory.  It would
                     be prudent to sequence the DNA to confirm the accuracy of
                     your records.  The sequence specified by this feature was
                     actually found to be:  CGAGGCGGCA TAAATCGCCG TGACGATC."
                     /label="Correction-134"
     primer_bind     6078..6103
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     662 bases.  Perfect match Tm = 60.7 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is agcgagaaga atcataatgg ggaagg."
                     /label="HGTI-1828_Max=662"
     misc_feature    6125..6237
                     /Description="SV40 5172..41, contains origin of
                     replication, from pMD cassette."
                     /label="SV40_ori"
     primer_bind     complement(6150..6176)
                     /Description="Add-musFLT-mIg Plasmid record #912  Max
                     recorded sequencing read = 618 bases.  Perfect match Tm =
                     59.3 C, although only 17 nt are required to match. The
                     actual sequence of the primer is tccagaagta gtgaggaggc
                     ttttttg."
                     /label="HGTI-8_Max=618"
ORIGIN      
        1 agcttggccc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
       61 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgggaccga tccagcctcc cctcgaagct gatcctgaga acttcagggt
      781 gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa gttcatgtca
      841 taggaagggg agaagtaaca gggtacacat attgaccaaa tcagggtaat tttgcatttg
      901 taattttaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattctg acactatgaa
     1321 gtgccttttg tacttagcct ttttattcat tggggtgaat tgcaagttca ccatagtttt
     1381 tccacacaac caaaaaggaa actggaaaaa tgttccttct aattaccatt attgcccgtc
     1441 aagctcagat ttaaattggc ataatgactt aataggcaca gccttacaag tcaaaatgcc
     1501 caagagtcac aaggctattc aagcagacgg ttggatgtgt catgcttcca aatgggtcac
     1561 tacttgtgat ttccgctggt atggaccgaa gtatataaca cattccatcc gatccttcac
     1621 tccatctgta gaacaatgca aggaaagcat tgaacaaacg aaacaaggaa cttggctgaa
     1681 tccaggcttc cctcctcaaa gttgtggata tgcaactgtg acggatgccg aagcagtgat
     1741 tgtccaggtg actcctcacc atgtgctggt tgatgaatac acaggagaat gggttgattc
     1801 acagttcatc aacggaaaat gcagcaatta catatgcccc actgtccata actctacaac
     1861 ctggcattct gactataagg tcaaagggct atgtgattct aacctcattt ccatggacat
     1921 caccttcttc tcagaggacg gagagctatc atccctggga aaggagggca cagggttcag
     1981 aagtaactac tttgcttatg aaactggagg caaggcctgc aaaatgcaat actgcaagca
     2041 ttggggagtc agactcccat caggtgtctg gttcgagatg gctgataagg atctctttgc
     2101 tgcagccaga ttccctgaat gcccagaagg gtcaagtatc tctgctccat ctcagacctc
     2161 agtggatgta agtctaattc aggacgttga gaggatcttg gattattccc tctgccaaga
     2221 aacctggagc aaaatcagag cgggtcttcc aatctctcca gtggatctca gctatcttgc
     2281 tcctaaaaac ccaggaaccg gtcctgcttt caccataatc aatggtaccc taaaatactt
     2341 tgagaccaga tacatcagag tcgatattgc tgctccaatc ctctcaagaa tggtcggaat
     2401 gatcagtgga actaccacag aaagggaact gtgggatgac tgggcaccat atgaagacgt
     2461 ggaaattgga cccaatggag ttctgaggac cagttcagga tataagtttc ctttatacat
     2521 gattggacat ggtatgttgg actccgatct tcatcttagc tcaaaggctc aggtgttcga
     2581 acatcctcac attcaagacg ctgcttcgca acttcctgat gatgagagtt tattttttgg
     2641 tgatactggg ctatccaaaa atccaatcga gcttgtagaa ggttggttca gtagttggaa
     2701 aagctctatt gcctcttttt tctttatcat agggttaatc attggactat tcttggttct
     2761 ccgagttggt atccatcttt gcattaaatt aaagcacacc aagaaaagac agatttatac
     2821 agacatagag atgaaccgac ttggaaagta actcaaatcc tgcacaacag attcttcatg
     2881 tttggaccaa atcaacttgt gataccatgc tcaaagaggc ctcaattata tttgagtttt
     2941 taatttttat gaaaaaaaaa aaaaaaacgg aattccgcgg gcggccgctc tagaggatcc
     3001 aagcttatcg ataccgtcga cctcgagggc ccagatctaa ttcaccccac cagtgcaggc
     3061 tgcctatcag aaagtggtgg ctggtgtggc taatgccctg gcccacaagt atcactaagc
     3121 tcgctttctt gctgtccaat ttctattaaa ggttcctttg ttccctaagt ccaactacta
     3181 aactggggga tattatgaag ggccttgagc atctggattc tgcctaataa aaaacattta
     3241 ttttcattgc aatgatgtat ttaaattatt tctgaatatt ttactaaaaa gggaatgtgg
     3301 gaggtcagtg catttaaaac ataaagaaat gaagagctag ttcaaacctt gggaaaatac
     3361 actatatctt aaactccatg aaagaaggtg aggctgcaaa cagctaatgc acattggcaa
     3421 cagcccctga tgcctatgcc ttattcatcc ctcagaaaag gattcaagta gaggcttgat
     3481 ttggaggtta aagttttgct atgctgtatt ttacattact tattgtttta gctgtcctca
     3541 tgaatgtctt ttcactaccc atttgcttat cctgcatctc tcagccttga ctccactcag
     3601 ttctcttgct tagagatacc acctttcccc tgaagtgttc cttccatgtt ttacggcgag
     3661 atggtttctc ctcgcctggc cactcagcct tagttgtctc tgttgtctta tagaggtcta
     3721 cttgaagaag gaaaaacagg gggcatggtt tgactgtcct gtgagccctt cttccctgcc
     3781 tcccccactc acagtgaccc ggaatccctc gacatggcag tctagatcat tcttgaagac
     3841 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     3901 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3961 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4021 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4081 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4141 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4201 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     4261 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     4321 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     4381 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     4441 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     4501 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     4561 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     4621 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4681 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     4741 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     4801 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     4861 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     4921 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4981 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5041 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5101 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5161 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5221 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5281 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5341 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5401 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5461 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5521 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5581 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5641 ggcggagcct atggaaaaac gccagcaacg gatgcgccgc gtgcggctgc tggagatggc
     5701 ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa
     5761 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt
     5821 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat
     5881 agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc
     5941 cccgtgacga tcagcggtcc aatgatcgaa gttaggctgg taagagccgc gagcgatcct
     6001 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc
     6061 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc
     6121 ggggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     6181 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatg
//
