LOCUS       HDM-Hgpm2               8910 bp    DNA     circular UNA 01-MAY-2004
DEFINITION  codon optimized version of the HIV gagpol fusion gene in an HDM
            expression.
ACCESSION   urn.local.alexbalazs.lcu-67t2cao
VERSION     urn.local.alexbalazs.lcu-67t2cao
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    9..679
                     /Description="CMV promoter.  Transcription starts with
                     next base.  5 prime end represents complete extent of the
                     CMV promoter in MDM based plasmids, which corresponds to
                     positions 471..1141 of hCMVie genbank record accession
                     X03922."
                     /label="CMV"
                     /note="Geneious type: promoter prokaryotic"
     misc_feature    96..679
                     /Description="CMV promoter.  Transcription starts with
                     next base.  5 prime end starts at Spe I site only.  The
                     CMV region in this construct may extend further upstream."
                     /label="CMV"
                     /note="Geneious type: promoter prokaryotic"
     primer_bind     151..178
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     638 bases.  Perfect match Tm = 58.7 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is agttccgcgt tacataactt acggtaaa."
                     /label="HGTI-1832_Max=638"
     primer_bind     complement(236..260)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     672 bases.  Perfect match Tm = 59.1 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ccctattggc gttactatgg gaaca."
                     /label="HGTI-1863_Max=672"
     primer_bind     complement(536..560)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     485 bases.  Perfect match Tm = 58.0 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is aaacaaactc ccattgacgt caatg."
                     /label="HGTI-1864_Max=485"
     primer_bind     561..593
                     /Description="sequencing pHD from 3xx end  Max recorded
                     sequencing read = 707 bases. Perfect match Tm = 71.5 C,
                     although only 17 nt are required to match.  The actual
                     sequence of the primer is tggcaccaaa atcaacggga ctttccaaaa
                     tgt."
                     /label="HGTI-2021_Max=707"
     primer_bind     612..652
                     /Description="?  Max recorded sequencing read = 162 bases.
                      Perfect match Tm = 76.1 C, although only 17 nt are
                     required to match.  The actual sequence of the primer is
                     gcgcactagt catatggtag gcgtgtacgg tgggaggtct a."
                     /label="HGTI-1220_Max=162"
     primer_bind     617..638
                     /Description="Sequencing primers for pHJ1  Max recorded
                     sequencing read = 311 bases. Perfect match Tm = 62.5 C,
                     although only 17 nt are required to match.  The actual
                     sequence of the primer is GCAAATGGGC GGTAGGCGTG TA."
                     /label="HGTI-1204_Max=311"
     misc_feature    628..679
                     /Description="CMV TATA box sequences, from -52 to -1. 
                     Wild type, with Sac I site."
                     /label="CMV_TATA"
     primer_bind     complement(662..703)
                     /Description="linker from SacI of CMV bridging into BglII
                     of HIV R.  Max recorded sequencing read =  bases.  Perfect
                     match Tm = 74.8 C, although only 17 nt are required to
                     match.  The actual sequence of the primer is gatcTGGTCT
                     AACCAGAGAG ACCCcggttc actaaacgAG CT."
                     /label="HGTI-1192_Max="
     misc_feature    680..750
                     /Description="5xx untranslated region from CMV immediate
                     early promoter.  The 3 prime end corresponds to the full
                     extent of CMV sequences in the MDM constructs."
                     /label="CMV_5xxUTR"
     primer_bind     692..715
                     /Description="PCR or sequencing primer located in CMV
                     sequence just after 5xx end of RNA transcript.  Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     63.9 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GAGACGCCAT CCACGCTGTT
                     TTGA."
                     /label="HGTI-761_Max="
     primer_bind     751..775
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     595 bases.  Perfect match Tm = 58.7 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is cctcgaagct gatcctgaga acttc."
                     /label="HGTI-1834_Max=595"
     misc_feature    779..1254
                     /Description="Human B-globin 2nd intron, Rsa I deleted."
                     /label="B-globin_intron"
     primer_bind     complement(833..860)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     636 bases.  Perfect match Tm = 59.3 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is tgttacttct ccccttccta tgacatga."
                     /label="HGTI-1865_Max=636"
     primer_bind     complement(952..981)
                     /Description="sequencing primer in globin intron facing
                     left.  Max recorded sequencing read = 610 bases.  Perfect
                     match Tm = 53.9 C, although only 17 nt are required to
                     match.  The actual sequence of the primer is gaaagaaaga
                     gattagggaa agtattagaa ."
                     /label="HGTI-2022_Max=610"
     primer_bind     1051..1082
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     639 bases.  Perfect match Tm = 58.2 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is gttaaggcaa tagcaatatt tctgcatata aa."
                     /label="HGTI-1835_Max=639"
     primer_bind     complement(1135..1160)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     639 bases.  Perfect match Tm = 59.3 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is gcagaatggt agctggattg tagctg."
                     /label="HGTI-1866_Max=639"
     primer_bind     1209..1243
                     /Description="New 5xx sequencing primer for B-globin
                     constructs.  Same position as JSL-70, but longer with a Tm
                     at 66C.  Max recorded sequencing read = 783 bases. 
                     Perfect match Tm = 65.5 C, although only 17 nt are
                     required to match. The actual sequence of the primer is
                     GGCCCTTTTG CTAATCATGT TCATACCTCT TATCT."
                     /label="HGTI-1732_Max=783"
     primer_bind     1224..1244
                     /Description="Jeng Shin designed sequencing primer @ 5xx
                     end of MDM2b polylinker facing towards gene.  xx723 is
                     longer, higher Tm versionxx.  Max recorded sequencing read
                     = 0 bases.  Perfect match Tm = 40.3 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is CATGTTCATA CCTCTTATCT T."
                     /label="HGTI-1278_Max=0"
     primer_bind     1226..1253
                     /Description="sFlt clone Add-musFlt-mIg: sequencing ORF 
                     Max recorded sequencing read = 0 bases.  Perfect match Tm
                     = 59.3 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is TGTTCATACC TCTTATCTTC
                     CTCCCACA."
                     /label="HGTI-2144_Max=0"
     CDS             1318..2817
                     /Description="Codon optimized gag gene, from pHDMHgpm2."
                     /label="HIV_gag_co"
     CDS             1321..1713
                     /Description="HIV codon-opt. matrix protein from gag. 
                     NL4-3."
                     /label="MA_p17"
     primer_bind     1324..1343
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read = 0 bases.  Perfect match Tm = 68.5 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is gcccgcgcct ccgtgctgtc ."
                     /label="HGTI-480_Max=0"
     primer_bind     1583..1606
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 66.3 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is accagcgcat cgacgtgaag gaca."
                     /label="HGTI-481_Max="
     primer_bind     complement(1600..1618)
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 55.1 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is gggcctcctt ggtgtcctt."
                     /label="HGTI-495_Max="
     CDS             1714..2406
                     /Description="HIV NL4-3 codon-opt. capsid p24 protein from
                     gag."
                     /label="CA_p24"
     primer_bind     1822..1845
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 66.6 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is atccccatgt tctccgccct gtcc."
                     /label="HGTI-482_Max="
     primer_bind     complement(1830..1847)
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 55.6 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is tcggacaggg cggagaac."
                     /label="HGTI-496_Max="
     primer_bind     1941..1970
                     /Description="PCR primer amplifies pMDA.HIVgp mam, yet
                     inserts PstI site from position 1946 of pMDA.HIVgp jtg. 
                     Max recorded sequencing read =  bases.  Perfect match Tm =
                     78.5 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is aGCtGCaGAG TGGGACCGCC
                     TGCACCCCGT ."
                     /label="HGTI-742_Max="
     primer_bind     2115..2134
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 54.8 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is catcctgggc ctgaacaaga ."
                     /label="HGTI-483_Max="
     primer_bind     complement(2146..2166)
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 57.3 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is caggatggag gtgggggagt a."
                     /label="HGTI-497_Max="
     primer_bind     2328..2348
                     /Description="use in pALTER derivative pA1xx3xx  Max
                     recorded sequencing read = bases.  Perfect match Tm = 75.4
                     C, although only 17 nt are required to match. The actual
                     sequence of the primer is gggccccggc gccaccctgg a."
                     /label="HGTI-400_Max="
     primer_bind     2407..2430
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 68 C, although only 17
                     nt are required to match.  The actual sequence of the
                     primer is gccgaggcca tgtcccaagt cacc."
                     /label="HGTI-484_Max="
     CDS             2407..2448
                     /Description="HIV p2 codon-opt. protein from gag"
                     /label="p2"
     primer_bind     complement(2416..2439)
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 67.2 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is ggcggggttg gtgacttggg acat."
                     /label="HGTI-498_Max="
     primer_bind     2435..2458
                     /Description="For sequencing dedicated mammalianized HIV
                     gag constructs.  Max recorded sequencing read =  bases. 
                     Perfect match Tm = 65.0 C, although only 17 nt are
                     required to match.  The actual sequence of the primer is
                     CCGCCACCAT CATGATCCAG AAGG."
                     /label="HGTI-522_Max="
     CDS             2449..2613
                     /Description="HIV NL4-3 codon-opt. nucleocapsid protein
                     from gag."
                     /label="NC_p7"
     CDS             2614..2661
                     /Description="HIV NL4-3 codon-opt. p1 protein from gag."
                     /label="p1"
     primer_bind     complement(2614..2633)
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 50.3 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is ggccagatct tccctaaaaa ."
                     /label="HGTI-499_Max="
     CDS             2619..5621
                     /Description="Codon optimized pol gene, from pHDMHgpm2."
                     /label="HIV_pol_co"
     primer_bind     complement(2637..2661)
                     /Description="Sequencing primers for sequencing gagpol of
                     HIV strain NL4-3.  Max recorded sequencing read =  bases. 
                     Perfect match Tm = 64.7 C, although only 17 nt are
                     required to match.  The actual sequence of the primer is
                     AAAATTCCCT GGCCTTCCCT TGTGG."
                     /label="HGTI-1016_Max="
     CDS             2662..2817
                     /Description="HIV NL4-3  codon-opt. p6 protein from gag."
                     /label="p6"
     primer_bind     2666..2700
                     /Description="Sequencing primers for sequencing gagpol of
                     HIV strain NL4-3.  Max recorded sequencing read =  bases. 
                     Perfect match Tm = 75.5 C, although only 17 nt are
                     required to match.  The actual sequence of the primer is
                     AGAGCAGACC AGAGCCAACA GCCCCACCAG AAGAG."
                     /label="HGTI-996_Max="
     primer_bind     2677..2700
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 63.9 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is gagccaacag ccccaccaga agag."
                     /label="HGTI-485_Max="
     CDS             2781..3077
                     /Description="HIV NL4-3  codon-opt. protease protein from
                     pol."
                     /label="PR_HIV"
     primer_bind     complement(2788..2810)
                     /Description="For sequencing dedicated gagpol
                     mammalianized constructs.  Max recorded sequencing read = 
                     bases.  Perfect match Tm = 63.3 C, although only 17 nt are
                     required to match.  The actual sequence of the primer is
                     gaggggtcgc tgccaaagag tga."
                     /label="HGTI-525_Max="
     primer_bind     complement(2790..2810)
                     /Description="Sequencing primers for sequencing gagpol of
                     HIV strain NL4-3.  Max recorded sequencing read =  bases. 
                     Perfect match Tm = 59.2 C, although only 17 nt are
                     required to match.  The actual sequence of the primer is
                     GAGGGGTCGC TGCCAAAGAG T."
                     /label="HGTI-1017_Max="
     primer_bind     complement(2887..2906)
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 68.7 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is ccagcggccg ggcaggttca ."
                     /label="HGTI-500_Max="
     primer_bind     2923..2944
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 69.3 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is gcggcatcgg cggcttcatc aa."
                     /label="HGTI-486_Max="
     primer_bind     2959..2983
                     /Description="Series of oligos for pALTER mutagenesis
                     repair of HIVgagpol clone p2xx3a.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 63.4 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is accagatcct gatcgagatc tgcgg."
                     /label="HGTI-437_Max="
     primer_bind     3017..3044
                     /Description="Series of oligos for pALTER mutagenesis
                     repair of HIVgagpol clone p2xx3a.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 75.7 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is cacccccgtg aacatcatcg gccgcaac."
                     /label="HGTI-438_Max="
     primer_bind     3045..3075
                     /Description="Series of oligos for pALTER mutagenesis
                     repair of HIVgagpol clone p2xx3a.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 74.1 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is ctgctgaccc agatcggctg caccctgaac t."
                     /label="HGTI-439_Max="
     primer_bind     3076..3104
                     /Description="Series of oligos for pALTER mutagenesis
                     repair of HIVgagpol clone p2xx3a.  Max recorded sequencing
                     read = 137 bases.  Perfect match Tm = 77.4 C, although
                     only 17 nt are required to match.  The actual sequence of
                     the primer is tccccatctc ccccatcgag accgtgccc."
                     /label="HGTI-440_Max=137"
     CDS             3078..4397
                     /Description="HIV NL4-3  codon-opt. reverse transcriptase
                     protein, p51 subunit."
                     /label="RT_p51"
     primer_bind     3163..3186
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 61.1 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is agaagatcaa ggccctggtg gaga."
                     /label="HGTI-487_Max="
     primer_bind     complement(3198..3218)
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 53.7 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is gatcttgccc tccttctcca t."
                     /label="HGTI-501_Max="
     primer_bind     3365..3396
                     /Description="PCR primer for fusing mammalianized HIV
                     gagpol with wt, this time after the protease region.  Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     74.6 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CCCTGCAGGC CTGAAGCAGA
                     AGAAGTCCGT GA."
                     /label="HGTI-764_Max="
     primer_bind     3460..3483
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 68.2 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is ccgccttcac catcccctcc atca."
                     /label="HGTI-488_Max="
     primer_bind     complement(3487..3504)
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 59.1 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is ggatgccggg ggtctcgt."
                     /label="HGTI-502_Max="
     primer_bind     3718..3736
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 55.8 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is tcaccacccc cgacaagaa."
                     /label="HGTI-489_Max="
     primer_bind     complement(3749..3781)
                     /Description="repairs last remaining 3xx3 mutations  Max
                     recorded sequencing read = bases.  Perfect match Tm = 79.4
                     C, although only 17 nt are required to match. The actual
                     sequence of the primer is TGCAGCTCGT AGCCCATCCA CAGGAAGGGG
                     GGC."
                     /label="HGTI-472_Max="
     primer_bind     complement(3900..3920)
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 59.0 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is cttgcacagc tggcggactt t."
                     /label="HGTI-503_Max="
     primer_bind     complement(3970..4000)
                     /Description="repairs last remaining 3xx3 mutations  Max
                     recorded sequencing read = 0 bases.  Perfect match Tm =
                     81.2 C, although only 17 nt are required to match. The
                     actual sequence of the primer is TCGCGGTTCT CGGCCAGCTC
                     CAGCTCGGCC T."
                     /label="HGTI-473_Max=0"
     primer_bind     4146..4169
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 68.2 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is atgaagggcg cccacaccaa cgac."
                     /label="HGTI-490_Max="
     primer_bind     complement(4238..4258)
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 57.4 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is tggatgggca gcttgaactt g."
                     /label="HGTI-504_Max="
     CDS             4398..4757
                     /Description="HIV NL4-3  codon-opt. RNaseH domain of
                     reverse transcriptase, in pol."
                     /label="RNaseH"
     primer_bind     4478..4501
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 69.2 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is gcccctgacc gacaccacca acca."
                     /label="HGTI-491_Max="
     primer_bind     4575..4614
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     81.3 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CAATATGCAT TGGGCATCAT
                     CCAGGCCCAG CCCGACAAGT ."
                     /label="HGTI-262_Max="
     primer_bind     complement(4595..4634)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     85.5 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GGACACCAGC TCGGACTCGG
                     ACTTGTCGGG CTGGGCCTGG ."
                     /label="HGTI-315_Max="
     primer_bind     4615..4654
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     81.2 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CCGAGTCCGA GCTGGTGTCC
                     CAGATCATCG AGCAGCTGAT ."
                     /label="HGTI-263_Max="
     primer_bind     complement(4635..4674)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     73.6 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GGTACACCTT CTCCTTCTTG
                     ATCAGCTGCT CGATGATCTG ."
                     /label="HGTI-314_Max="
     primer_bind     4655..4694
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     82.4 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CAAGAAGGAG AAGGTGTACC
                     TGGCCTGGGT GCCCGCCCAC ."
                     /label="HGTI-264_Max="
     primer_bind     complement(4675..4714)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     91.9 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is TCGTTGCCGC CGATGCCCTT
                     GTGGGCGGGC ACCCAGGCCA ."
                     /label="HGTI-313_Max="
     primer_bind     complement(4710..4742)
                     /Description="2 PCR repair oligos to repair the RNase H
                     domain of pHDMHgpm  Max recorded sequencing read =  bases.
                      Perfect match Tm = 80.3 C, although only 17 nt are
                     required to match.  The actual sequence of the primer is
                     GCCGGCGGAC ACCAGcttGT CCACCTGCTC GTT."
                     /label="HGTI-1135_Max="
     primer_bind     4710..4742
                     /Description="2 PCR repair oligos to repair the RNase H
                     domain of pHDMHgpm  Max recorded sequencing read =  bases.
                      Perfect match Tm = 80.3 C, although only 17 nt are
                     required to match.  The actual sequence of the primer is
                     AACGAGCAGG TGGACaagCT GGTGTCCGCC GGC."
                     /label="HGTI-1134_Max="
     primer_bind     4735..4774
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     88.5 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CCGCCGGCAT CCGCAAGGTG
                     CTGTTCCTGG ACGGCATCGA ."
                     /label="HGTI-266_Max="
     primer_bind     complement(4755..4794)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     84.0 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CGTGCTCCTC CTGGGCCTTG
                     TCGATGCCGT CCAGGAACAG ."
                     /label="HGTI-311_Max="
     CDS             4758..5621
                     /Description="HIV NL4-3 codon-opt. integrase protein from
                     pol."
                     /label="IN_HIV"
     primer_bind     4773..4796
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 65.8 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is gacaaggccc aggaggagca cgag."
                     /label="HGTI-492_Max="
     primer_bind     4775..4814
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     78.8 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CAAGGCCCAG GAGGAGCACG
                     AGAAGTACCA CTCCAACTGG ."
                     /label="HGTI-267_Max="
     primer_bind     complement(4795..4834)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     81.4 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is AAGTCGGAGG CCATGGCGCG
                     CCAGTTGGAG TGGTACTTCT ."
                     /label="HGTI-310_Max="
     primer_bind     4815..4854
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     89.3 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CGCGCCATGG CCTCCGACTT
                     CAACCTGCCC CCCGTGGTGG ."
                     /label="HGTI-268_Max="
     primer_bind     complement(4829..4846)
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read = 0 bases.  Perfect match Tm = 55.0 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is gggggcaggt tgaagtcg."
                     /label="HGTI-506_Max=0"
     primer_bind     complement(4835..4874)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     86.7 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GGAGGCCACG ATCTCCTTGG
                     CCACCACGGG GGGCAGGTTG ."
                     /label="HGTI-309_Max="
     primer_bind     4855..4894
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     82.4 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CCAAGGAGAT CGTGGCCTCC
                     TGCGACAAGT GCCAGCTGAA ."
                     /label="HGTI-269_Max="
     primer_bind     complement(4875..4914)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm = 88
                     C, although only 17 nt are required to match.  The actual
                     sequence of the primer is GGCCGTGCAT GGCCTCGCCC TTCAGCTGGC
                     ACTTGTCGCA ."
                     /label="HGTI-308_Max="
     primer_bind     4895..4934
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     90.8 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GGGCGAGGCC ATGCACGGCC
                     AGGTGGACTG CTCCCCCGGC ."
                     /label="HGTI-270_Max="
     primer_bind     complement(4915..4954)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     85.1 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GTGCAGTCCA GCTGCCAGAT
                     GCCGGGGGAG CAGTCCACCT ."
                     /label="HGTI-307_Max="
     primer_bind     4935..4974
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     83.4 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is ATCTGGCAGC TGGACTGCAC
                     CCACCTGGAG GGCAAGGTGA ."
                     /label="HGTI-271_Max="
     primer_bind     complement(4955..4994)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     86.1 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CACGTGCACG GCCACCAGGA
                     TCACCTTGCC CTCCAGGTGG ."
                     /label="HGTI-306_Max="
     primer_bind     4975..5014
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     87.4 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is TCCTGGTGGC CGTGCACGTG
                     GCCTCCGGCT ACATCGAGGC ."
                     /label="HGTI-272_Max="
     primer_bind     complement(4995..5034)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     86.4 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is TCTCGGCGGG GATCACCTCG
                     GCCTCGATGT AGCCGGAGGC ."
                     /label="HGTI-305_Max="
     primer_bind     5015..5054
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read = 405 bases.  Perfect match Tm =
                     86.2 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CGAGGTGATC CCCGCCGAGA
                     CCGGCCAGGA GACCGCCTAC ."
                     /label="HGTI-273_Max=405"
     primer_bind     complement(5035..5074)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     85.1 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CCGGCCAGCT TCAGCAGGAA
                     GTAGGCGGTC TCCTGGCCGG ."
                     /label="HGTI-304_Max="
     primer_bind     5055..5094
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     88.3 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is TTCCTGCTGA AGCTGGCCGG
                     CCGCTGGCCC GTGAAGACCG ."
                     /label="HGTI-274_Max="
     primer_bind     complement(5075..5114)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     87.7 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GGAGCCGTTG TCGGTGTGCA
                     CGGTCTTCAC GGGCCAGCGG ."
                     /label="HGTI-303_Max="
     primer_bind     5086..5108
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 61.6 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is tgaagaccgt gcacaccgac aac."
                     /label="HGTI-493_Max="
     primer_bind     5095..5134
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     83.2 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is TGCACACCGA CAACGGCTCC
                     AACTTCACCT CCACCACCGT ."
                     /label="HGTI-275_Max="
     primer_bind     complement(5115..5154)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     84.4 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CCCACCAGCA GGCGGCCTTC
                     ACGGTGGTGG AGGTGAAGTT ."
                     /label="HGTI-302_Max="
     primer_bind     5135..5174
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     85.8 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GAAGGCCGCC TGCTGGTGGG
                     CCGGCATCAA GCAGGAGTTC ."
                     /label="HGTI-276_Max="
     primer_bind     complement(5155..5194)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     83.8 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is TGGGGGTTGT AGGGGATGCC
                     GAACTCCTGC TTGATGCCGG ."
                     /label="HGTI-301_Max="
     primer_bind     5175..5214
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     83.1 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GGCATCCCCT ACAACCCCCA
                     GTCCCAGGGC GTGATCGAGT ."
                     /label="HGTI-277_Max="
     primer_bind     complement(5184..5202)
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read = 541 bases.  Perfect match Tm = 54.3 C, although
                     only 17 nt are required to match.  The actual sequence of
                     the primer is cctgggactg ggggttgta."
                     /label="HGTI-507_Max=541"
     primer_bind     complement(5195..5234)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     79.5 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CTTCAGCTCC TTGTTCATGG
                     ACTCGATCAC GCCCTGGGAC ."
                     /label="HGTI-300_Max="
     primer_bind     5255..5294
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read = 424 bases.  Perfect match Tm =
                     87.3 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CGACCAGGCC GAGCACCTGA
                     AGACCGCCGT GCAGATGGCC ."
                     /label="HGTI-279_Max=424"
     primer_bind     complement(5275..5314)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read = 487 bases.  Perfect match Tm =
                     81.9 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is TTGAAGTTGT GGATGAACAC
                     GGCCATCTGC ACGGCGGTCT ."
                     /label="HGTI-298_Max=487"
     primer_bind     5295..5334
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     84.4 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GTGTTCATCC ACAACTTCAA
                     GCGCAAGGGC GGCATCGGCG ."
                     /label="HGTI-280_Max="
     primer_bind     complement(5315..5354)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     92.6 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GCGCTCGCCG GCGGAGTAGC
                     CGCCGATGCC GCCCTTGCGC ."
                     /label="HGTI-297_Max="
     primer_bind     5319..5341
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read =  bases.  Perfect match Tm = 69.3 C, although only
                     17 nt are required to match.  The actual sequence of the
                     primer is aagggcggca tcggcggcta ctc."
                     /label="HGTI-494_Max="
     primer_bind     5335..5374
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     86.6 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GCTACTCCGC CGGCGAGCGC
                     ATCGTGGACA TCATCGCCAC ."
                     /label="HGTI-281_Max="
     primer_bind     complement(5355..5394)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     81.4 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GCTCCTTGGT CTGGATGTCG
                     GTGGCGATGA TGTCCACGAT ."
                     /label="HGTI-296_Max="
     primer_bind     5375..5414
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read = 530 bases.  Perfect match Tm =
                     78.2 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CGACATCCAG ACCAAGGAGC
                     TGCAGAAGCA GATCACCAAG ."
                     /label="HGTI-282_Max=530"
     primer_bind     complement(5395..5434)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     77.3 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is TACACGCGGA AGTTCTGGAT
                     CTTGGTGATC TGCTTCTGCA ."
                     /label="HGTI-295_Max="
     primer_bind     5415..5454
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     81.5 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is ATCCAGAACT TCCGCGTGTA
                     CTACCGCGAC TCCCGCGACC ."
                     /label="HGTI-283_Max="
     primer_bind     complement(5435..5474)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     89.6 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GGCGGGGCCC TTCCACACGG
                     GGTCGCGGGA GTCGCGGTAG ."
                     /label="HGTI-294_Max="
     primer_bind     5455..5494
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     88.3 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CCGTGTGGAA GGGCCCCGCC
                     AAGCTGCTGT GGAAGGGCGA ."
                     /label="HGTI-284_Max="
     primer_bind     complement(5475..5514)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     86.2 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CCTGGATCAC CACGGCGCCC
                     TCGCCCTTCC ACAGCAGCTT ."
                     /label="HGTI-293_Max="
     primer_bind     5495..5534
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     83.5 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GGGCGCCGTG GTGATCCAGG
                     ACAACTCCGA CATCAAGGTG ."
                     /label="HGTI-285_Max="
     primer_bind     complement(5515..5554)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     85.8 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is TTGGCCTTGC GGCGGGGCAC
                     CACCTTGATG TCGGAGTTGT ."
                     /label="HGTI-292_Max="
     primer_bind     complement(5524..5545)
                     /Description="HGPM is for HIV gag pol mammalianized.  The
                     series is for sequencing primers.  Max recorded sequencing
                     read = 412 bases.  Perfect match Tm = 68.3 C, although
                     only 17 nt are required to match.  The actual sequence of
                     the primer is cggcggggca ccaccttgat gt."
                     /label="HGTI-508_Max=412"
     primer_bind     5535..5574
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     87.6 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GTGCCCCGCC GCAAGGCCAA
                     GATCATCCGC GACTACGGCA ."
                     /label="HGTI-286_Max="
     primer_bind     complement(5555..5594)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     85.0 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GTCGTCGCCG GCCATCTGCT
                     TGCCGTAGTC GCGGATGATC ."
                     /label="HGTI-291_Max="
     primer_bind     5575..5614
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     89.9 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is AGCAGATGGC CGGCGACGAC
                     TGCGTGGCCT CCCGCCAGGA ."
                     /label="HGTI-287_Max="
     primer_bind     complement(5595..5634)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     83.1 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is TTTCCATGTG TTAGTCCTCG
                     TCCTGGCGGG AGGCCACGCA ."
                     /label="HGTI-290_Max="
     primer_bind     5615..5654
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     66.1 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is CGAGGACTAA CACATGGAAA
                     AGATTAGTAA AACACCATAG ."
                     /label="HGTI-288_Max="
     primer_bind     complement(5635..5681)
                     /Description="All oligos in the 54 oligo set will be used
                     together in a shuffling expt to reconstruct the HIV
                     integrase gene using optimized mammalian codon usage. Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     76.8 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is ctctgcatgc GGATCCTCTA
                     GAGCGGCCTA TGGTGTTTTA CTAATCT."
                     /label="HGTI-289_Max="
     misc_feature    5710..6469
                     /Description="From 3rd exon of human beta globin, contains
                     polyA sequences commonly used in HGTI vector lab
                     constructs."
                     /label="beta_globin_pA"
     primer_bind     complement(5756..5773)
                     /Description="Jeng Shin designed sequencing primer @ 3xx
                     end of MDM2b polylinker facing towards gene.New
                     incarnation is 3149, reordered 1xx29xx01.  Max recorded
                     sequencing read =  bases.  Perfect match Tm = 54.2 C,
                     although only 17 nt are required to match.  The actual
                     sequence of the primer is gccagggcat tagccaca."
                     /label="HGTI-1279_Max="
     primer_bind     5879..5907
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     379 bases.  Perfect match Tm = 59.8 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is agcatctgga ttctgcctaa taaaaaaca."
                     /label="HGTI-1843_Max=379"
     polyA_site      5897..5921
                     /Description="Axxs added after these bases in globin
                     message."
                     /label="polyA_site"
     primer_bind     complement(5964..5988)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     692 bases.  Perfect match Tm = 58.1 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ttaaatgcac tgacctccca cattc."
                     /label="HGTI-1874_Max=692"
     primer_bind     6180..6213
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     643 bases.  Perfect match Tm = 59.1 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ttttacatta cttattgttt tagctgtcct catg."
                     /label="HGTI-1844_Max=643"
     primer_bind     complement(6259..6289)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     610 bases.  Perfect match Tm = 59.5 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is tatctctaag caagagaact gagtggagtc a."
                     /label="HGTI-1875_Max=610"
     misc_feature    6445..6469
                     /Description="End of globin sequences in typical pMD
                     constructs."
                     /label="End_of_globin"
     misc_feature    6483..6520
                     /Description="Extra EcoR I site of pMD-old-gag-pol.  This
                     sequence is definitely not in all MDM constructs. 
                     Probably only exists in a few older constructs prior to
                     the MDM2b set.  This sequence has been found to be
                     erroneous for some plasmids in our laboratory.  It would
                     be prudent to sequence the DNA to confirm the accuracy of
                     your records.  The sequence specified by this feature was
                     actually found to be:  ACATGGCAGT CTAGAATTCT TGAAGACGAA
                     AGGGCC."
                     /label="Correction-143"
     primer_bind     complement(6522..6552)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     711 bases.  Perfect match Tm = 58.3 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is gacattaacc tataaaaata ggcgtatcac g."
                     /label="HGTI-1856_Max=711"
     CDS             6709..7566
                     /Description="B-lactamase, confers ampicillin resistance,
                     no Pst I."
                     /label="bla"
     primer_bind     6743..6763
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     647 bases.  Perfect match Tm = 60.2 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ccttttttgc ggcattttgc c."
                     /label="HGTI-1886_Max=647"
     primer_bind     complement(6829..6852)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     661 bases.  Perfect match Tm = 58.0 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is atccagttcg atgtaaccca ctcg."
                     /label="HGTI-1855_Max=661"
     primer_bind     7043..7070
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     654 bases.  Perfect match Tm = 60.0 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is cggatggcat gacagtaaga gaattatg."
                     /label="HGTI-1885_Max=654"
     primer_bind     complement(7132..7152)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     650 bases.  Perfect match Tm = 58.4 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is agcggttagc tccttcggtc c."
                     /label="HGTI-1854_Max=650"
     primer_bind     7343..7367
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     593 bases.  Perfect match Tm = 59.6 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ataaagttgc aggaccactt ctgcg."
                     /label="HGTI-1884_Max=593"
     primer_bind     complement(7431..7452)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 0
                     bases.  Perfect match Tm = 61.0 C, although only 17 nt are
                     required to match.  The actual sequence of the primer is
                     ccccagtgct gcaatgatac cg."
                     /label="HGTI-1853_Max=0"
     primer_bind     7643..7671
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     564 bases.  Perfect match Tm = 58.2 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is tgaagatcct ttttgataat ctcatgacc."
                     /label="HGTI-1883_Max=564"
     primer_bind     complement(7723..7752)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     372 bases.  Perfect match Tm = 58.8 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is aaaaggatct caagaagatc ctttgatctt ."
                     /label="HGTI-1852_Max=372"
     misc_feature    7865..7908
                     /Description="Tag for High copy plasmid replication
                     xxpUC18xx"
                     /label="High_copy"
     primer_bind     7943..7970
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     565 bases.  Perfect match Tm = 60.5 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is catacctcgc tctgctaatc ctgttacc."
                     /label="HGTI-1882_Max=565"
     primer_bind     complement(8034..8052)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     601 bases.  Perfect match Tm = 60.1 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is cgaccgctgc gccttatcc."
                     /label="HGTI-1851_Max=601"
     primer_bind     8243..8268
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     511 bases.  Perfect match Tm = 59 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is atctttatag tcctgtcggg tttcgc."
                     /label="HGTI-1881_Max=511"
     primer_bind     8751..8776
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     662 bases.  Perfect match Tm = 60.7 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is agcgagaaga atcataatgg ggaagg."
                     /label="HGTI-1828_Max=662"
     misc_feature    8798..8910
                     /Description="SV40 5172..41, contains origin of
                     replication, from pMD cassette."
                     /label="SV40_ori"
     primer_bind     complement(8823..8849)
                     /Description="Add-musFLT-mIg Plasmid record #912  Max
                     recorded sequencing read = 618 bases.  Perfect match Tm =
                     59.3 C, although only 17 nt are required to match. The
                     actual sequence of the primer is tccagaagta gtgaggaggc
                     ttttttg."
                     /label="HGTI-8_Max=618"
ORIGIN      
        1 agcttggccc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
       61 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgggaccga tccagcctcc cctcgaagct gatcctgaga acttcagggt
      781 gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa gttcatgtca
      841 taggaagggg agaagtaaca gggtacacat attgaccaaa tcagggtaat tttgcatttg
      901 taattttaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattcta gactgccatg
     1321 ggcgcccgcg cctccgtgct gtccggcggc gagctggaca agtgggagaa gatccgcctg
     1381 cgccccggcg gcaagaagca gtacaagctg aagcacatcg tgtgggcctc ccgcgagctg
     1441 gagcgcttcg ccgtgaaccc cggcctgctg gagacctccg agggctgccg ccagatcctg
     1501 ggccagctgc agccctccct gcaaaccggc tccgaggagc tgcgctccct gtacaacacc
     1561 atcgccgtgc tgtactgcgt gcaccagcgc atcgacgtga aggacaccaa ggaggccctg
     1621 gacaagatcg aggaggagca gaacaagtcc aagaagaagg cccagcaggc cgccgccgac
     1681 accggcaaca actcccaggt gtcccagaac taccccatcg tgcagaacct gcagggccag
     1741 atggtgcacc aggccatctc cccccgcacc ctgaacgcct gggtgaaggt ggtggaggag
     1801 aaggccttct cccccgaagt catccccatg ttctccgccc tgtccgaggg cgccaccccc
     1861 caggacctga acaccatgct gaacaccgtg ggcggccacc aggccgccat gcagatgctg
     1921 aaggagacca tcaacgagga ggccgccgag tgggaccgcc tgcaccccgt gcacgccggc
     1981 cccatcgccc ccggccagat gcgcgagccc cgcggctccg acatcgccgg caccacctcc
     2041 accctgcaag agcagatcgg ctggatgacc cacaaccccc ccatccccgt gggcgagatc
     2101 tacaagcgct ggatcatcct gggcctgaac aagatcgtgc gcatgtactc ccccacctcc
     2161 atcctggaca tccgccaggg ccccaaggag cccttccgcg actacgtgga ccgcttctac
     2221 aagaccctgc gcgccgagca ggcctcccag gaggtaaaga actggatgac cgagaccctg
     2281 ctggtgcaga acgccaaccc cgactgcaag accatcctga aggccctggg ccccggcgcc
     2341 accctggagg agatgatgac cgcctgccag ggcgtgggcg gccccggcca caaggcccgc
     2401 gtgctggccg aggccatgtc ccaagtcacc aaccccgcca ccatcatgat ccagaagggc
     2461 aacttccgca accagcgcaa gaccgtgaag tgcttcaact gcggcaagga gggccacatc
     2521 gccaagaact gccgcgcccc ccgcaagaag ggctgctgga agtgcggcaa ggagggccac
     2581 cagatgaaag attgtactga gagacaggct aattttttag ggaagatctg gccttcccac
     2641 aagggaaggc cagggaattt tcttcagagc agaccagagc caacagcccc accagaagag
     2701 agcttcaggt ttggggaaga gacaacaact ccctctcaga agcaggagcc gatagacaag
     2761 gaactgtatc ctttagcttc cctcagatca ctctttggca gcgacccctc gtcacaataa
     2821 agatcggtgg ccagctgaag gaggccctgc tggacaccgg cgccgacgac accgtgctgg
     2881 aggagatgaa cctgcccggc cgctggaagc ccaagatgat cggcggcatc ggcggcttca
     2941 tcaaagtccg ccagtacgac cagatcctga tcgagatctg cggccacaag gccatcggca
     3001 ccgtgctggt gggccccacc cccgtgaaca tcatcggccg caacctgctg acccagatcg
     3061 gctgcaccct gaacttcccc atctccccca tcgagaccgt gcccgtgaag ctgaagcccg
     3121 gcatggacgg ccccaaagtc aagcagtggc ccctgaccga ggagaagatc aaggccctgg
     3181 tggagatctg caccgagatg gagaaggagg gcaagatctc caagatcggc cccgagaacc
     3241 cctacaacac ccccgtgttc gccatcaaga agaaggactc caccaagtgg cgcaagctgg
     3301 tggacttccg cgagctgaac aagcgcaccc aggacttctg ggaggtgcag ctgggcatcc
     3361 cccaccccgc cggcctgaag cagaagaagt ccgtgaccgt gctggacgtg ggcgacgcct
     3421 acttctccgt gcccctggac aaggacttcc gcaagtacac cgccttcacc atcccctcca
     3481 tcaacaacga gacccccggc atccgctacc agtacaacgt gctgccccag ggctggaagg
     3541 gctcccccgc catcttccag tgctccatga ccaagatcct ggagcccttc cgcaagcaga
     3601 accccgacat cgtgatctac cagtacatgg acgacctgta cgtgggctcc gacctggaga
     3661 tcggccagca ccgcaccaag atcgaggagc tgcgccagca cctgctgcgc tggggcttca
     3721 ccacccccga caagaagcac cagaaggagc cccccttcct gtggatgggc tacgagctgc
     3781 accccgacaa gtggaccgtg cagcccatcg tgctgcccga gaaggactcc tggaccgtga
     3841 acgacatcca gaagctggtg ggcaagctga actgggcctc ccagatctac gccggcatca
     3901 aagtccgcca gctgtgcaag ctgctgcgcg gcaccaaggc cctgaccgag gtggtgcccc
     3961 tgaccgagga ggccgagctg gagctggccg agaaccgcga gatcctgaag gagcccgtgc
     4021 acggcgtgta ctacgacccc tccaaggacc tgatcgccga gatccagaag cagggccagg
     4081 gccagtggac ctaccagatc taccaggagc ccttcaagaa cctgaagacc ggcaaatacg
     4141 cccgcatgaa gggcgcccac accaacgacg tgaagcagct gaccgaggcc gtgcagaaga
     4201 tcgccaccga gtccatcgtg atctggggca agactcccaa gttcaagctg cccatccaga
     4261 aggagacctg ggaggcctgg tggaccgagt actggcaggc cacctggatc cccgagtggg
     4321 agttcgtgaa cacccccccc ctggtgaagc tgtggtacca gctggagaag gagcccatca
     4381 tcggcgccga gaccttctac gtggacggcg ccgccaaccg cgagaccaag ctgggcaagg
     4441 ccggctacgt gaccgaccgc ggccgccaga aggtggtgcc cctgaccgac accaccaacc
     4501 agaagaccga gctgcaggcc atccacctgg ccctgcaaga ctccggcctg gaggtgaaca
     4561 tcgtgaccga ctcccagtat gcattgggca tcatccaggc ccagcccgac aagtccgagt
     4621 ccgagctggt gtcccagatc atcgagcagc tgatcaagaa ggagaaggtg tacctggcct
     4681 gggtgcccgc ccacaagggc atcggcggca acgagcaggt ggacaagctg gtgtccgccg
     4741 gcatccgcaa ggtgctgttc ctggacggca tcgacaaggc ccaggaggag cacgagaagt
     4801 accactccaa ctggcgcgcc atggcctccg acttcaacct gccccccgtg gtggccaagg
     4861 agatcgtggc ctcctgcgac aagtgccagc tgaagggcga ggccatgcac ggccaggtgg
     4921 actgctcccc cggcatctgg cagctggact gcacccacct ggagggcaag gtgatcctgg
     4981 tggccgtgca cgtggcctcc ggctacatcg aggccgaggt gatccccgcc gagaccggcc
     5041 aggagaccgc ctacttcctg ctgaagctgg ccggccgctg gcccgtgaag accgtgcaca
     5101 ccgacaacgg ctccaacttc acctccacca ccgtgaaggc cgcctgctgg tgggccggca
     5161 tcaagcagga gttcggcatc ccctacaacc cccagtccca gggcgtgatc gagtccatga
     5221 acaaggagct gaagaagatc atcggccaag tccgcgacca ggccgagcac ctgaagaccg
     5281 ccgtgcagat ggccgtgttc atccacaact tcaagcgcaa gggcggcatc ggcggctact
     5341 ccgccggcga gcgcatcgtg gacatcatcg ccaccgacat ccagaccaag gagctgcaga
     5401 agcagatcac caagatccag aacttccgcg tgtactaccg cgactcccgc gaccccgtgt
     5461 ggaagggccc cgccaagctg ctgtggaagg gcgagggcgc cgtggtgatc caggacaact
     5521 ccgacatcaa ggtggtgccc cgccgcaagg ccaagatcat ccgcgactac ggcaagcaga
     5581 tggccggcga cgactgcgtg gcctcccgcc aggacgagga ctaacacatg gaaaagatta
     5641 gtaaaacacc ataggccgct ctagaggatc caagcttatc gataccgtcg acctcgaggg
     5701 cccagatcta attcacccca ccagtgcagg ctgcctatca gaaagtggtg gctggtgtgg
     5761 ctaatgccct ggcccacaag tatcactaag ctcgctttct tgctgtccaa tttctattaa
     5821 aggttccttt gttccctaag tccaactact aaactggggg atattatgaa gggccttgag
     5881 catctggatt ctgcctaata aaaaacattt attttcattg caatgatgta tttaaattat
     5941 ttctgaatat tttactaaaa agggaatgtg ggaggtcagt gcatttaaaa cataaagaaa
     6001 tgaagagcta gttcaaacct tgggaaaata cactatatct taaactccat gaaagaaggt
     6061 gaggctgcaa acagctaatg cacattggca acagcccctg atgcctatgc cttattcatc
     6121 cctcagaaaa ggattcaagt agaggcttga tttggaggtt aaagttttgc tatgctgtat
     6181 tttacattac ttattgtttt agctgtcctc atgaatgtct tttcactacc catttgctta
     6241 tcctgcatct ctcagccttg actccactca gttctcttgc ttagagatac cacctttccc
     6301 ctgaagtgtt ccttccatgt tttacggcga gatggtttct cctcgcctgg ccactcagcc
     6361 ttagttgtct ctgttgtctt atagaggtct acttgaagaa ggaaaaacag ggggcatggt
     6421 ttgactgtcc tgtgagccct tcttccctgc ctcccccact cacagtgacc cggaatccct
     6481 cgacatggca gtctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     6541 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     6601 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6661 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     6721 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     6781 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     6841 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     6901 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     6961 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7021 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7081 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7141 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7201 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     7261 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7321 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7381 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7441 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7501 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7561 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7621 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7681 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7741 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7801 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7861 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7921 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7981 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     8041 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     8101 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     8161 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     8221 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     8281 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8341 ggagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg
     8401 gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt
     8461 gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca
     8521 ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac
     8581 ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccaatgatc
     8641 gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct
     8701 acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga
     8761 atcataatgg ggaaggccat ccagcctcgc gtcggggagc tttttgcaaa agcctaggcc
     8821 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     8881 tgcataaata aaaaaaatta gtcagccatg
//
