LOCUS       HDM-Tat1b               4829 bp    DNA     circular UNA 03-AUG-2006
DEFINITION  HIV tat 1b in HDM.
ACCESSION   urn.local.alexbalazs.jxi-67t2c80
VERSION     urn.local.alexbalazs.jxi-67t2c80
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    9..679
                     /Description="CMV promoter.  Transcription starts with
                     next base.  5 prime end represents complete extent of the
                     CMV promoter in MDM based plasmids, which corresponds to
                     positions 471..1141 of hCMVie genbank record accession
                     X03922."
                     /label="CMV"
                     /note="Geneious type: promoter prokaryotic"
     misc_feature    96..679
                     /Description="CMV promoter.  Transcription starts with
                     next base.  5 prime end starts at Spe I site only.  The
                     CMV region in this construct may extend further upstream."
                     /label="CMV"
                     /note="Geneious type: promoter prokaryotic"
     primer_bind     151..178
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     638 bases.  Perfect match Tm = 58.7 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is agttccgcgt tacataactt acggtaaa."
                     /label="HGTI-1832_Max=638"
     primer_bind     complement(236..260)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     672 bases.  Perfect match Tm = 59.1 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ccctattggc gttactatgg gaaca."
                     /label="HGTI-1863_Max=672"
     primer_bind     complement(536..560)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     485 bases.  Perfect match Tm = 58.0 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is aaacaaactc ccattgacgt caatg."
                     /label="HGTI-1864_Max=485"
     primer_bind     561..593
                     /Description="sequencing pHD from 3xx end  Max recorded
                     sequencing read = 707 bases. Perfect match Tm = 71.5 C,
                     although only 17 nt are required to match.  The actual
                     sequence of the primer is tggcaccaaa atcaacggga ctttccaaaa
                     tgt."
                     /label="HGTI-2021_Max=707"
     primer_bind     612..652
                     /Description="?  Max recorded sequencing read = 162 bases.
                      Perfect match Tm = 76.1 C, although only 17 nt are
                     required to match.  The actual sequence of the primer is
                     gcgcactagt catatggtag gcgtgtacgg tgggaggtct a."
                     /label="HGTI-1220_Max=162"
     primer_bind     617..638
                     /Description="Sequencing primers for pHJ1  Max recorded
                     sequencing read = 311 bases. Perfect match Tm = 62.5 C,
                     although only 17 nt are required to match.  The actual
                     sequence of the primer is GCAAATGGGC GGTAGGCGTG TA."
                     /label="HGTI-1204_Max=311"
     misc_feature    628..679
                     /Description="CMV TATA box sequences, from -52 to -1. 
                     Wild type, with Sac I site."
                     /label="CMV_TATA"
     primer_bind     complement(662..703)
                     /Description="linker from SacI of CMV bridging into BglII
                     of HIV R.  Max recorded sequencing read =  bases.  Perfect
                     match Tm = 74.8 C, although only 17 nt are required to
                     match.  The actual sequence of the primer is gatcTGGTCT
                     AACCAGAGAG ACCCcggttc actaaacgAG CT."
                     /label="HGTI-1192_Max="
     misc_feature    680..750
                     /Description="5xx untranslated region from CMV immediate
                     early promoter.  The 3 prime end corresponds to the full
                     extent of CMV sequences in the MDM constructs."
                     /label="CMV_5xxUTR"
     primer_bind     692..715
                     /Description="PCR or sequencing primer located in CMV
                     sequence just after 5xx end of RNA transcript.  Max
                     recorded sequencing read =  bases.  Perfect match Tm =
                     63.9 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is GAGACGCCAT CCACGCTGTT
                     TTGA."
                     /label="HGTI-761_Max="
     primer_bind     751..775
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     595 bases.  Perfect match Tm = 58.7 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is cctcgaagct gatcctgaga acttc."
                     /label="HGTI-1834_Max=595"
     misc_feature    779..1254
                     /Description="Human B-globin 2nd intron, Rsa I deleted."
                     /label="B-globin_intron"
     primer_bind     complement(833..860)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     636 bases.  Perfect match Tm = 59.3 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is tgttacttct ccccttccta tgacatga."
                     /label="HGTI-1865_Max=636"
     primer_bind     complement(952..981)
                     /Description="sequencing primer in globin intron facing
                     left.  Max recorded sequencing read = 610 bases.  Perfect
                     match Tm = 53.9 C, although only 17 nt are required to
                     match.  The actual sequence of the primer is gaaagaaaga
                     gattagggaa agtattagaa ."
                     /label="HGTI-2022_Max=610"
     primer_bind     1051..1082
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     639 bases.  Perfect match Tm = 58.2 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is gttaaggcaa tagcaatatt tctgcatata aa."
                     /label="HGTI-1835_Max=639"
     primer_bind     complement(1135..1160)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     639 bases.  Perfect match Tm = 59.3 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is gcagaatggt agctggattg tagctg."
                     /label="HGTI-1866_Max=639"
     primer_bind     1209..1243
                     /Description="New 5xx sequencing primer for B-globin
                     constructs.  Same position as JSL-70, but longer with a Tm
                     at 66C.  Max recorded sequencing read = 783 bases. 
                     Perfect match Tm = 65.5 C, although only 17 nt are
                     required to match. The actual sequence of the primer is
                     GGCCCTTTTG CTAATCATGT TCATACCTCT TATCT."
                     /label="HGTI-1732_Max=783"
     primer_bind     1224..1244
                     /Description="Jeng Shin designed sequencing primer @ 5xx
                     end of MDM2b polylinker facing towards gene.  xx723 is
                     longer, higher Tm versionxx.  Max recorded sequencing read
                     = 0 bases.  Perfect match Tm = 40.3 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is CATGTTCATA CCTCTTATCT T."
                     /label="HGTI-1278_Max=0"
     primer_bind     1226..1253
                     /Description="sFlt clone Add-musFlt-mIg: sequencing ORF 
                     Max recorded sequencing read = 0 bases.  Perfect match Tm
                     = 59.3 C, although only 17 nt are required to match.  The
                     actual sequence of the primer is TGTTCATACC TCTTATCTTC
                     CTCCCACA."
                     /label="HGTI-2144_Max=0"
     CDS             1325..1585
                     /Description="HIV Tat Protein"
                     /label="Tat1b"
     primer_bind     complement(1473..1497)
                     /Description="Sequencing primers for HIV strain HXB2
                     construct.  Max recorded sequencing read =  bases. 
                     Perfect match Tm = 64.9 C, although only 17 nt are
                     required to match.  The actual sequence of the primer is
                     GCTCTTCGTC GCTGTCTCCG CTTCT."
                     /label="HGTI-1121_Max="
     misc_feature    1629..2388
                     /Description="From 3rd exon of human beta globin, contains
                     polyA sequences commonly used in HGTI vector lab
                     constructs."
                     /label="beta_globin_pA"
     primer_bind     complement(1675..1692)
                     /Description="Jeng Shin designed sequencing primer @ 3xx
                     end of MDM2b polylinker facing towards gene.New
                     incarnation is 3149, reordered 1xx29xx01.  Max recorded
                     sequencing read =  bases.  Perfect match Tm = 54.2 C,
                     although only 17 nt are required to match.  The actual
                     sequence of the primer is gccagggcat tagccaca."
                     /label="HGTI-1279_Max="
     primer_bind     1798..1826
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     379 bases.  Perfect match Tm = 59.8 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is agcatctgga ttctgcctaa taaaaaaca."
                     /label="HGTI-1843_Max=379"
     polyA_site      1816..1840
                     /Description="Axxs added after these bases in globin
                     message."
                     /label="polyA_site"
     primer_bind     complement(1883..1907)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     692 bases.  Perfect match Tm = 58.1 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ttaaatgcac tgacctccca cattc."
                     /label="HGTI-1874_Max=692"
     primer_bind     2099..2132
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     643 bases.  Perfect match Tm = 59.1 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ttttacatta cttattgttt tagctgtcct catg."
                     /label="HGTI-1844_Max=643"
     primer_bind     complement(2178..2208)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     610 bases.  Perfect match Tm = 59.5 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is tatctctaag caagagaact gagtggagtc a."
                     /label="HGTI-1875_Max=610"
     misc_feature    2364..2388
                     /Description="End of globin sequences in typical pMD
                     constructs."
                     /label="End_of_globin"
     misc_feature    2402..2439
                     /Description="Extra EcoR I site of pMD-old-gag-pol.  This
                     sequence is definitely not in all MDM constructs. 
                     Probably only exists in a few older constructs prior to
                     the MDM2b set.  This sequence has been found to be
                     erroneous for some plasmids in our laboratory.  It would
                     be prudent to sequence the DNA to confirm the accuracy of
                     your records.  The sequence specified by this feature was
                     actually found to be:  ACATGGCAGT CTAGAATTCT TGAAGACGAA
                     AGGGCC."
                     /label="Correction-143"
     primer_bind     complement(2441..2471)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     711 bases.  Perfect match Tm = 58.3 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is gacattaacc tataaaaata ggcgtatcac g."
                     /label="HGTI-1856_Max=711"
     CDS             2628..3485
                     /Description="B-lactamase, confers ampicillin resistance,
                     no Pst I."
                     /label="bla"
     primer_bind     2662..2682
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     647 bases.  Perfect match Tm = 60.2 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ccttttttgc ggcattttgc c."
                     /label="HGTI-1886_Max=647"
     primer_bind     complement(2748..2771)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     661 bases.  Perfect match Tm = 58.0 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is atccagttcg atgtaaccca ctcg."
                     /label="HGTI-1855_Max=661"
     primer_bind     2962..2989
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     654 bases.  Perfect match Tm = 60.0 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is cggatggcat gacagtaaga gaattatg."
                     /label="HGTI-1885_Max=654"
     primer_bind     complement(3051..3071)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     650 bases.  Perfect match Tm = 58.4 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is agcggttagc tccttcggtc c."
                     /label="HGTI-1854_Max=650"
     primer_bind     3262..3286
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     593 bases.  Perfect match Tm = 59.6 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is ataaagttgc aggaccactt ctgcg."
                     /label="HGTI-1884_Max=593"
     primer_bind     complement(3350..3371)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read = 0
                     bases.  Perfect match Tm = 61.0 C, although only 17 nt are
                     required to match.  The actual sequence of the primer is
                     ccccagtgct gcaatgatac cg."
                     /label="HGTI-1853_Max=0"
     primer_bind     3562..3590
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     564 bases.  Perfect match Tm = 58.2 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is tgaagatcct ttttgataat ctcatgacc."
                     /label="HGTI-1883_Max=564"
     primer_bind     complement(3642..3671)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     372 bases.  Perfect match Tm = 58.8 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is aaaaggatct caagaagatc ctttgatctt ."
                     /label="HGTI-1852_Max=372"
     misc_feature    3784..3827
                     /Description="Tag for High copy plasmid replication
                     xxpUC18xx"
                     /label="High_copy"
     primer_bind     3862..3889
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     565 bases.  Perfect match Tm = 60.5 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is catacctcgc tctgctaatc ctgttacc."
                     /label="HGTI-1882_Max=565"
     primer_bind     complement(3953..3971)
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     601 bases.  Perfect match Tm = 60.1 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is cgaccgctgc gccttatcc."
                     /label="HGTI-1851_Max=601"
     primer_bind     4162..4187
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     511 bases.  Perfect match Tm = 59 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is atctttatag tcctgtcggg tttcgc."
                     /label="HGTI-1881_Max=511"
     primer_bind     4670..4695
                     /Description="Full plasmid sequencing for
                     Add-BGFP-FasL-Ires-FADD  Max recorded sequencing read =
                     662 bases.  Perfect match Tm = 60.7 C, although only 17 nt
                     are required to match.  The actual sequence of the primer
                     is agcgagaaga atcataatgg ggaagg."
                     /label="HGTI-1828_Max=662"
     misc_feature    4717..4829
                     /Description="SV40 5172..41, contains origin of
                     replication, from pMD cassette."
                     /label="SV40_ori"
     primer_bind     complement(4742..4768)
                     /Description="Add-musFLT-mIg Plasmid record #912  Max
                     recorded sequencing read = 618 bases.  Perfect match Tm =
                     59.3 C, although only 17 nt are required to match. The
                     actual sequence of the primer is tccagaagta gtgaggaggc
                     ttttttg."
                     /label="HGTI-8_Max=618"
ORIGIN      
        1 agcttggccc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
       61 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgggaccga tccagcctcc cctcgaagct gatcctgaga acttcagggt
      781 gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa gttcatgtca
      841 taggaagggg agaagtaaca gggtacacat attgaccaaa tcagggtaat tttgcatttg
      901 taattttaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattccg cgggcggccg
     1321 cgaaatggag ccagtagatc ctagactaga gccctggaag catccaggaa gtcagcctaa
     1381 aactgcttgt accacttgct attgtaaaaa gtgttgcttt cattgccaag tttgtttcac
     1441 aacaaaagcc ttaggcatct cctatggcag gaagaagcgg agacagcgac gaagacctcc
     1501 tcaaggcagt cagactcatc aagtttctct atcaaagcaa cccacctccc aaccccgagg
     1561 ggacccgaca ggcccgaagg aataggatcc aagcttatcg ataccgtcga cctcgagggc
     1621 ccagatctaa ttcaccccac cagtgcaggc tgcctatcag aaagtggtgg ctggtgtggc
     1681 taatgccctg gcccacaagt atcactaagc tcgctttctt gctgtccaat ttctattaaa
     1741 ggttcctttg ttccctaagt ccaactacta aactggggga tattatgaag ggccttgagc
     1801 atctggattc tgcctaataa aaaacattta ttttcattgc aatgatgtat ttaaattatt
     1861 tctgaatatt ttactaaaaa gggaatgtgg gaggtcagtg catttaaaac ataaagaaat
     1921 gaagagctag ttcaaacctt gggaaaatac actatatctt aaactccatg aaagaaggtg
     1981 aggctgcaaa cagctaatgc acattggcaa cagcccctga tgcctatgcc ttattcatcc
     2041 ctcagaaaag gattcaagta gaggcttgat ttggaggtta aagttttgct atgctgtatt
     2101 ttacattact tattgtttta gctgtcctca tgaatgtctt ttcactaccc atttgcttat
     2161 cctgcatctc tcagccttga ctccactcag ttctcttgct tagagatacc acctttcccc
     2221 tgaagtgttc cttccatgtt ttacggcgag atggtttctc ctcgcctggc cactcagcct
     2281 tagttgtctc tgttgtctta tagaggtcta cttgaagaag gaaaaacagg gggcatggtt
     2341 tgactgtcct gtgagccctt cttccctgcc tcccccactc acagtgaccc ggaatccctc
     2401 gacatggcag tctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     2461 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     2521 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     2581 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     2641 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     2701 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     2761 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     2821 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     2881 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2941 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     3001 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3061 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3121 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3181 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3241 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     3301 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     3361 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     3421 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     3481 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     3541 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     3601 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3661 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3721 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3781 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3841 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3901 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3961 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4021 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4081 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4141 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4201 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4261 gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg
     4321 ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg
     4381 aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac
     4441 cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc
     4501 cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg
     4561 aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta
     4621 cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa
     4681 tcataatggg gaaggccatc cagcctcgcg tcggggagct ttttgcaaaa gcctaggcct
     4741 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
     4801 gcataaataa aaaaaattag tcagccatg
//
