LOCUS       Exported                2781 bp ds-DNA     circular SYN 09-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Turkewitz Lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Dec 9, 2020 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    10..46
                     /label=B-globin 5'UTR
     primer_bind     26..47
                     /label=37A_BGlobin 5'UTR F
     misc_feature    join(56..102,104..123)
                     /label=3xFLAG
     misc_feature    56..58
                     /label=START
     misc_feature    130..148
                     /label=linker
     misc_feature    149..1990
                     /label=ythdf1
     primer_bind     809..827
                     /label=8673_ythdf1_flag_seq1
     misc_feature    1430..1834
                     /label=YTH domain
     primer_bind     complement(1468..1487)
                     /label=8674_ythdf1_flag_seq2
     misc_feature    2000..2152
                     /label=B-globin 3utr
     primer_bind     complement(2103..2124)
                     /label=37b-3'UTR Bglobin
     misc_feature    2153..2202
                     /label=A30/C30
     gap             2762..2781
                     /estimated_length=20
ORIGIN
        1 aaactgatct ttgagagctc agataacgct caactttggc agatccgcgg ccgccatgga
       61 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
      121 caagaattca ggttctggct caggatcgtc taccaccagt attgacccac agacatcaaa
      181 agggcaagac gctaaagtgc aaaatggctc tcttcatcag aaggaaacgg tccacgacaa
      241 tgactttgaa ccatacctca ctggccagtc taatccgaat aacagttacc aatccatgac
      301 cgacccatac ctgtctagct actacgcccc ctcaattggg ttcccgtacc ccctcagcga
      361 ggctccctgg tccacaggtg gagacccgcc gatcccatac ctcactccgt atgcccccct
      421 cagcaatgga gaccatcact tcatgcatga cactgtgttt ggacagccgg ggggtctggg
      481 cagtagcatt tatccacacc gctttaactt tttccccgaa aaccctgcgt tttctgcctg
      541 gggtacgagt ggctcccagg gccagcagac tcagagttct gcatatgggg gcagctacag
      601 ttacccgccc agttctttag gtggaaccct ggtgccagac gggcaaacag gcttccctag
      661 tgacaccctg aataaggcac caggtatgaa tagcttggag cagggcatgg tggggctgaa
      721 gatcggtggt gatgtgactg gaggtggttc cggagttaag acggtgggct ccgtgattgg
      781 aggagggccg gtggctgcgg cggtggccac gggtaatggt gggactccta ttggactgcc
      841 gccccctaag cccacatctt gggcagccat tgctagcaaa ccggccaagc ctcagcaact
      901 gaaggtgaag agtaagccag ggatgcccat ggctggaggc accctgcctc cgccgcccat
      961 caaacacaac atggacattg gcacttggga taataagggg cctgtgacca aagtggcatc
     1021 atcattgcca cctcaccacc accaccagca gcagcctccc ctccactccc agggtcacct
     1081 tccacctcac cctcatggac tgccaccccc acctcagccc ccaatgccgt ctgcacaatc
     1141 acttgcccag cagatggcca tgcagggccc accccctccg cagccttacc agaaccacgc
     1201 ccctgctcct cccccgcaaa ctcggtgggt tgccccacgc aatcgcaacc ctggttatgg
     1261 tggtggagga agcgtggaca gcagtggctc ctcgagtggt ggaggggtcg gcaatggagg
     1321 cggaggtggc ccaccgggct ccggcgccat cgagtcccat cccgtcttag aaaagctgcg
     1381 tgcggcacac agctacaacc ccaaggagtt cgattggaac ctgaaaaatg gccgggtgtt
     1441 catcattaag agctactctg aggacgacat ccatcgctcc atcaagtact ccatctggtg
     1501 cagcacggaa catggaaaca agcggctgga ttctgcgttt cgtgccatca acggcaaagg
     1561 tcctgtctac ctgctgttca gcgtcaacgg cagcggacac ttctgtggcg tggcggagat
     1621 gcgttcgcct gttgactacg gcaccagtgc cggtgtttgg gcgcaggaca agtggaaggg
     1681 caaatttgat gtggactggc tgtttgttaa agacgtgccc aacagtcagc tcaggcacat
     1741 ccgcctggag aacaatgaca acaagcccgt gaccaactcg cgtgacacac aggaggtgcc
     1801 tctggaaaag gccaagcagg tgctgaagat catcgctacc tacaaacaca caacctccat
     1861 cttcgatgac ttctcacatt acgagaaacg gcaggaggag gaagaggtgg taagaaagaa
     1921 tcttgagcct gctcctatcc agaaccgatc ccgactggat caagaacgcc agaaccgaaa
     1981 taaacaatag ctcgagaata attcggatct ggttaccact aaaccagcct caagaacacc
     2041 cgaatggagt ctctaagcta cataatacca acttacactt tacaaaatgt tgtcccccaa
     2101 aatgtagcca ttcgtatctg ctcctaataa aaagaaagtt tcttcacatt ctaaaaaaaa
     2161 aaaaaaaaaa aaaaaaaaaa aaaccccccc ccccaccccc cctaaggtcg actctaaaag
     2221 atccccgggc gagctcgaat taattcccaa accttgtcaa aaccttttcc cggggaaaaa
     2281 tttttatccc cctcaaattt cccaacaaaa aacaaacccg aaaacataaa gttaaaaacc
     2341 ctggggggcc caaaaaattg agccacatca ccatatatgg gtttgccccc atcgccccct
     2401 tttctcttgg gaaaaacttg cttggcgccc tcttattata aatcccccca caccccggga
     2461 aaaaagggtt ttgttttttt ggggggcttc ttcctttccc cctcccaaaa aaaccccttc
     2521 cccccctgtt ttttcttgcc cgcggagaag aatatattct ctctcaaaca gggggttaaa
     2581 atattttcca cacaatacca aggaattacc aaaaaaaaaa aaaatttttt taaaaaaccc
     2641 cacaaaaaac ccacccccca taaaaaaaac cccttttttt ttgttttttt tcttttttcc
     2701 cccccccctc ccccaaaaaa aaaaaaaaaa aaaaaatttt ttcctaaaaa tttagaaaaa
     2761 cnnnnnnnnn nnnnnnnnnn n
//
