LOCUS       Exported                2778 bp ds-DNA     circular SYN 09-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Turkewitz Lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Dec 9, 2020 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    10..46
                     /label=B-globin 5'UTR
     primer_bind     26..47
                     /label=37A_BGlobin 5'UTR F
     misc_feature    join(56..102,104..123)
                     /label=3xFLAG
     misc_feature    56..58
                     /label=START
     misc_feature    130..148
                     /label=linker
     misc_feature    149..1987
                     /label=ythdf2
     primer_bind     592..611
                     /label=8675_ythdf2_flag_seq1
     misc_feature    1412..1816
                     /label=YTH domain
     primer_bind     complement(1446..1464)
                     /label=8676_ythdf2_flag_seq2
     misc_feature    1997..2149
                     /label=B-globin 3utr
     primer_bind     complement(2100..2121)
                     /label=37b-3'UTR Bglobin
     misc_feature    2150..2199
                     /label=A30/C30
     gap             2759..2778
                     /estimated_length=20
ORIGIN
        1 aaactgatct ttgagagctc agataacgct caactttggc agatccgcgg ccgccatgga
       61 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
      121 caagaattca ggttctggct caggatcgtc agccagcagc cttctggagc agagaccgaa
      181 aggccaagca aacaaagtac aaaacggagc tgttacccaa aaggatactt tgaacgatga
      241 tgagttcgag ccttacctga atgctcagcc cagacagagc aatgcctata cggccatgtc
      301 cgactcctac atgcccagtt actacagccc ctcaataggc ttcacctatt ccctgaatga
      361 agcggcatgg tcaacaggtg gagatccccc gatgccttac ctggcctctt atggacagct
      421 tagcaatggg gagcaccact ttctcccgga tgccatgttt ggacagtctg gagcactggg
      481 gaacaaccct ttcctaggac agcatggctt caacttcttt cccagtggga ttgactttcc
      541 cgcttggggc aacagcagct ctcagggaca gtccacacag agttcaggtt acagcagcag
      601 ttacgcctat gcccctagca cgctaggagg tgcaatgatt gacggacagt ctccctttgc
      661 agccaatgag ccactcaaca aggcagtggg gatgaatagc ttggatcagg gcatggcagg
      721 gcttaaaatt ggggcagggg acatggcacc aaaggttgtt ggttctggac ttcccggagg
      781 gccattgagt caggtgtcta cagctccaac catgcctcct gcctccatgg ctcctgcgaa
      841 aactgcttct tgggcggaca ttgccagcaa gccagccaaa ccacagccta aattgaaaac
      901 taagggggga cttggtggga caaatctgcc accacctcca ataaaacata acatggatat
      961 tgggacatgg gacaacaagg ggaatatgcc taaaccagca gccccacagc agacttccct
     1021 gcccactaat ggacagccac ccaatcagtc gtctcctcaa ccaggtgcta ctgctggtgg
     1081 ggtgcctcag ttgcccctca gcaatggaca gctagttcct cctactggac agcttgtcca
     1141 gcaccctctt cctccaggtg gccagcccgg tgctgttcca cctcaactct cccagggccc
     1201 accagcttcc caaccctccc agccaacccg ctgggtgccc ccgcgtaacc gtgccaacgg
     1261 ctttggggat gcagcaggtg gacctggcca atcccctccc aattcaggaa tgggtggcat
     1321 tactgtgcca gcagaacccc acccagttct agaaaaactg cgcatggtaa acaactacaa
     1381 tcccaaggac ttcgactgga accctaaaca tggtcgtgtc ttcatcatca agagttactc
     1441 tgaggacgat atccaccggt ccattaagta caatatctgg tgcagcacag aacatggcaa
     1501 taagcgtctg gacgctgcat accgttcgct agccaacaaa gggccactgt acctgctctt
     1561 cagtgtgaat ggcagtgggc acttctgtgg cgtggcggag atgcgctcgc ctgtggacta
     1621 caacacttgt gcaggcgtgt ggtcgcagga caagtggaag ggtcgtttcg atgtgcgctg
     1681 gatctttgtc aaggacgttc ccaacagcca actaaggcac attcgcttgg agaacaatga
     1741 gaataagccg gtaaccaact ctcgcgacac acaggaggtt cctctagaca aggcacgtca
     1801 agtgttgaag atcatcgcga gttacaagca caccacctcc atttttgacg acttctcaca
     1861 ctacgagaaa cgtcaggaag aggaggagag tgtaaaaaag gtggaggttc agggcagtga
     1921 cccgtactcc aataactcca gtcggagcca ctacagaatg caggatcgcc aaggacgtgt
     1981 aaagtaactc gagaataatt cggatctggt taccactaaa ccagcctcaa gaacacccga
     2041 atggagtctc taagctacat aataccaact tacactttac aaaatgttgt cccccaaaat
     2101 gtagccattc gtatctgctc ctaataaaaa gaaagtttct tcacattcta aaaaaaaaaa
     2161 aaaaaaaaaa aaaaaaaaaa cccccccccc caccccccct aaggtcgact ctaaaagatc
     2221 cccgggcgag ctcgaattaa ttcccaaacc ttgtcaaaac cttttcccgg ggaaaaattt
     2281 ttatccccct caaatttccc aacaaaaaac aaacccgaaa acataaagtt aaaaaccctg
     2341 gggggcccaa aaaattgagc cacatcacca tatatgggtt tgcccccatc gccccctttt
     2401 ctcttgggaa aaacttgctt ggcgccctct tattataaat ccccccacac cccgggaaaa
     2461 aagggttttg tttttttggg gggcttcttc ctttccccct cccaaaaaaa ccccttcccc
     2521 ccctgttttt tcttgcccgc ggagaagaat atattctctc tcaaacaggg ggttaaaata
     2581 ttttccacac aataccaagg aattaccaaa aaaaaaaaaa atttttttaa aaaaccccac
     2641 aaaaaaccca ccccccataa aaaaaacccc tttttttttg ttttttttct tttttccccc
     2701 ccccctcccc caaaaaaaaa aaaaaaaaaa aaattttttc ctaaaaattt agaaaaacnn
     2761 nnnnnnnnnn nnnnnnnn
//
