LOCUS       Exported                6716 bp ds-DNA     circular SYN 07-NOV-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   123
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 10, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     http://www.informaxinc.com/
            ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(join(146..416,4457..4499))
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSSARHWDQRSQYASIHWPSFYNVVTGKTLALPNLIALQH
                     IPLSPAGVIAKRPAPIALPNSCAA*MANGA*CGIFSLRICAVFHTAYGALSVQSALMPH
                     "
     primer_bind     352..375
                     /label=M13F(-47)
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     416..427
                     /label=P0843
                     /note="HS4 FWD"
     misc_feature    438..717
                     /label=HS4
     misc_feature    721..1000
                     /label=HS4
     primer_bind     complement(997..1043)
                     /label=P0830
                     /note="HS4 spacer"
     primer_bind     complement(1000..1023)
                     /label=P0844
                     /note="HS4 REV"
     primer_bind     1018..1043
                     /label=P0831
                     /note="attB-GA mutant (BxBI) FWD"
     misc_feature    1020..1023
                     /label=AACG
     gene            1024..1168
                     /gene="RPL30"
                     /gene_synonym="L30"
                     /label=RPL30
                     /note="ribosomal protein L30; Derived by automated
                     computational analysis using gene prediction method: 
                     BestRefSeq."
                     /db_xref="GeneID:6156"
                     /db_xref="HGNC:HGNC:10333"
                     /db_xref="MIM:180467"
     misc_feature    1024..1070
                     /label=attB-GA mutant (BxBI)
                     /label=attB (BxBI)
                     /note="Attachment Site Selection and Identity in Bxb1 
                     Serine Integrase-Mediated Site-Specific Recombination"
     misc_feature    1046
                     /label=GT dinucleotide
     misc_feature    1107
                     /label=BranchPoint
                     /note="Predicted by LaBranchoR"
     misc_feature    1110..1135
                     /label=Y-rich
     misc_feature    1136..1137
                     /label=AG
     mRNA            1138..1168
                     /gene="RPL30"
                     /gene_synonym="L30"
                     /product="ribosomal protein L30"
                     /label=RPL30 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq."
                     /note="/transcript_id=NM_000989.4"
                     /db_xref="Ensembl:ENST00000287038.8"
                     /db_xref="GeneID:6156"
                     /db_xref="HGNC:HGNC:10333"
                     /db_xref="MIM:180467"
     primer_bind     complement(1149..1178)
                     /label=P0832
                     /note="pRPL30 REV"
     primer_bind     1168..1192
                     /label=P0833
                     /note="E. coli sfGFP drop out FWD"
     promoter        1185..1314
                     /label=BBa_J72163 GlpT Promoter
     RBS             1315..1350
                     /label=sfGFP Ribosome Binding Site
     CDS             1351..2067
                     /label=sfGFP
     terminator      2068..2196
                     /label=BBa_B0015 Terminator
     primer_bind     complement(2184..2208)
                     /label=P0834
                     /note="E. coli sfGFP drop out REV"
     primer_bind     2198..2228
                     /label=P0835
                     /note="5'_Myb_IRES_FWD"
     misc_feature    2204..2207
                     /label=TGGC
     CDS             2208..2210
                     /codon_start=1
                     /label=Stop
                     /translation=""
     misc_RNA        2211..2408
                     /label=Myb_5'UTR
                     /note="Identification of a motif that mediates
                     polypyrimidine tract-binding
                     protein-dependent internal
                     ribosome entry
                     Sally A. Mitchell,1,3 Keith A. Spriggs,1,3 Martin Bushell,1
                     Joanne R. Evans,1 Mark Stoneley,1
                     John P.C. Le Quesne,1 Ruth V. Spriggs,2 and Anne E. 
                     Willis1,4
                     1
                     School of Pharmacy, University of Nottingham, University 
                     Park, Nottingham, NG7 2RD, UK; 2
                     European Bioinformatics
                     Institute, Wellcome Trust Genome Campus, Hinxton, 
                     Cambridge, CB10 1SD, UK"
     misc_feature    2211..2360
                     /label=Minimal IRES element
     misc_feature    2409..2412
                     /label=TATG
     misc_feature    2409
                     /label=frame shift
     CDS             2410..2472
                     /codon_start=1
                     /label=Ig Kappa Signal Sequence
                     /translation="METDTLLLWVLLLWVPGSTGD"
     CDS             2473..2505
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     2475..2540
                     /label=P0725
     primer_bind     complement(2486..2511)
                     /label=P0836
                     /note="T7-tag_REV"
     primer_bind     2508..2531
                     /label=P0837
                     /note="HaloTag_FWD"
     CDS             2512..3399
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     primer_bind     2512..2540
                     /label=HaloTag_3a_P01_F
     primer_bind     2512..2538
                     /label=P0727
     primer_bind     2512..2528
                     /label=P0052
     primer_bind     complement(2565..2584)
                     /label=QB5204
                     /note="Early_HALO_Rev"
     misc_feature    2794..2796
                     /label=G96
                     /note="This residue is mutated to cysteine in the ZM_nHalo 
                     fragment"
     primer_bind     complement(2923..2952)
                     /label=HaloTag_3a_P01_R
     primer_bind     2946..2974
                     /label=HaloTag_3a_P02_F
     primer_bind     complement(2950..2974)
                     /label=Halo_155_3_P01_R
     primer_bind     complement(2954..2985)
                     /label=P0053
     primer_bind     2972..2993
                     /label=P0054
     primer_bind     2988..3007
                     /label=QB4083
     primer_bind     complement(3310..3341)
                     /label=HaloTag_3a_P02_R
     primer_bind     complement(3313..3342)
                     /label=Halo_278_3_P01_R
     primer_bind     3336..3362
                     /label=HaloTag_3a_P03_F
     primer_bind     3350..3367
                     /label=QB4084
     primer_bind     complement(3352..3361)
                     /label=QB2197
     primer_bind     complement(3375..3405)
                     /label=HaloTag_3a_P03_R
     primer_bind     complement(3380..3399)
                     /label=P0279
     primer_bind     complement(3380..3399)
                     /label=P0132
                     /note="HaloTag_stop_EcoRI_REV"
     CDS             3406..3555
                     /codon_start=1
                     /label=PDGFR Transmembrane Domain
                     /translation="NAVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLW
                     QKKPR"
     primer_bind     3406..3427
                     /label=P0723
                     /note="PDGFR_TM_4a_F"
     primer_bind     complement(3523..3555)
                     /label=P0724
                     /note="PDGFR_TM_4a_R"
     primer_bind     complement(3539..3581)
                     /label=P0730
     CDS             3562..3624
                     /codon_start=1
                     /label=T2A
                     /translation="GSGEGRGSLLTCGDVEENPGP"
     primer_bind     3562..3581
                     /label=P0731
     primer_bind     complement(3605..3630)
                     /label=P0838
                     /note="T2A_REV"
     primer_bind     complement(3609..3645)
                     /label=P0826
                     /note="T2A-puro REV"
     primer_bind     3627..3650
                     /label=P0839
                     /note="Puro FWD"
     misc_feature    3627..3630
                     /label=TGGC
     CDS             3631..4227
                     /codon_start=1
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    4236..4456
                     /label=full_length_SV40_late_polyA
     polyA_signal    4308..4315
                     /label=USE3
                     /note="Upstream Sequence Element 3"
     misc_feature    4317..4324
                     /label=USE2
                     /note="Upstream Sequence Element 2"
     misc_feature    4337..4344
                     /label=USE1
                     /note="Upstream Sequence Element 1.Most important USE
                     "
     polyA_signal    4359..4364
                     /label=pA_signal
     polyA_site      4377
                     /label=PolyA Cleavage Site
     misc_feature    4382..4404
                     /label=DSE-U
                     /note="Downstream Sequence Element. U-rich"
     misc_feature    4409..4422
                     /label=DSE-G
                     /note="Downstream Sequence Element, G-rich"
     misc_feature    4426..4431
                     /label=DSE-U'
                     /note="Downstream Sequence Element, U-rich"
     primer_bind     complement(4435..4460)
                     /label=P0840
                     /note="flSV40late REV"
     primer_bind     complement(4495..4511)
                     /label=M13R
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4519..4535
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4543..4573)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4588..4609
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     4798..4819
                     /label=P0842
                     /note="ColE1 REV"
     rep_origin      complement(4897..5485)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5562..5584
                     /label=P0841
                     /note="Amp terminator FWD"
     CDS             complement(5656..6516)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6517..6621)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagactgtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatcactg
      421 cagatcgaat tcgagttggc gcgcctgtca ttctaaatct ctctttcagc ctaaagcttt
      481 ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa
      541 agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga
      601 tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg
      661 gggagggacg taattacatc cctgggggct ttgggagggg gctgtccccg tgagctccca
      721 ggcgcgcctg tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca
      781 ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc
      841 ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg
      901 accggagcgg agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac
      961 atccctgggg gctttggggg ggggctgtcc ccgtgagctc aataaaagag cccactacaa
     1021 acgggccggc ttgtcgacga cggcggactc cgtcgtcagg atcatccggg agctttgcgg
     1081 gttgccattt gtaactccgg atcctaaaat tcctgtcctg ttctctgtct cttctaggtt
     1141 gggggccgtc ccgctcctaa ggcaggaata tgtgagacca gaccgaaagt gaaacgtgat
     1201 ttcatgcgtc attttgaaca ttttgtaaat cttatttaat aatgtgtgcg gcaattcaca
     1261 tttaatttat gaatgttttc ttaacatcgc ggcaactcaa gaaacggcag gttcggatct
     1321 tagctactag agaaagagga gaaatactag atgcgtaaag gcgaagagct gttcactggt
     1381 gtcgtcccta ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt
     1441 ggcgagggtg aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact
     1501 ggtaaactgc cggttccttg gccgactctg gtaacgacgc tgacttatgg tgttcagtgc
     1561 tttgctcgtt atccggacca tatgaagcag catgacttct tcaagtccgc catgccggaa
     1621 ggctatgtgc aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg
     1681 gaagtgaaat ttgaaggcga taccctggta aaccgcattg agctgaaagg cattgacttt
     1741 aaagaggacg gcaatatcct gggccataag ctggaataca attttaacag ccacaatgtt
     1801 tacatcaccg ccgataaaca aaaaaatggc attaaagcga attttaaaat tcgccacaac
     1861 gtggaggatg gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat
     1921 ggtcctgttc tgctgccaga caatcactat ctgagcacgc aaagcgttct gtctaaagat
     1981 ccgaacgaga aacgcgatca tatggttctg ctggagttcg taaccgcagc gggcatcacg
     2041 catggtatgg atgaactgta caaatgacca ggcatcaaat aaaacgaaag gctcagtcga
     2101 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     2161 ctggctcacc ttcgggtggg cctttctgcg tttataggtc tcatggctag atatcaacct
     2221 gtttcctcct cctccttctc ctcctcctcc gtgacctcct cctcctcttt ctcctgagaa
     2281 acttcgcccc agcggtgcgg agcgccgctg cgcagccggg gagggacgca ggcaggcggc
     2341 gggcagcggg aggcggcagc ccggtgcggt ccccgcggct ctcggcggag ccccgcgccc
     2401 gccgcgccta tggagactga caccctcttg ctctgggtcc tgctgctgtg ggttccgggt
     2461 agtaccggtg acatggctag catgactggt ggacagcaaa tgggtggttc tgcagaaatc
     2521 ggtactggct ttccattcga cccccattat gtggaagtcc tgggcgagcg catgcactac
     2581 gtcgatgttg gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa cccgacctcc
     2641 tcctacgtgt ggcgcaacat catcccgcat gttgcaccga cccatcgctg cattgctcca
     2701 gacctgatcg gtatgggcaa atccgacaaa ccagacctgg gttatttctt cgacgaccac
     2761 gtccgcttca tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt
     2821 cacgactggg gctccgctct gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa
     2881 ggtattgcat ttatggagtt catccgccct atcccgacct gggacgaatg gccagaattt
     2941 gcccgcgaga ctttccaggc cttccgcacc accgacgtcg gccgcaagct gatcatcgat
     3001 cagaacgttt ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc
     3061 gagatggacc attaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc
     3121 ttcccaaacg agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa
     3181 tacatggact ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc
     3241 gttctgatcc caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct
     3301 gtggacatcg gcccgggtct gaatctgctg caagaggaca acccggacct gatcggcagc
     3361 gagatcgcgc gctggctgtc gacgctcgag atttccggcg gttctaacgc cgtgggccag
     3421 gatacgcagg aggtgatcgt cgtgccccac agcttgccct tcaaggtcgt ggttattagt
     3481 gctatcctgg cgttggtggt gctgactatt atctccctta tcatacttat catgctgtgg
     3541 caaaaaaaac cccgaggatc cggaagcgga gagggcagag gaagtctgct aacatgcggt
     3601 gacgtcgagg agaatcctgg acctggtggc accgagtaca agcctaccgt gcgcctggcc
     3661 actcgcgatg atgtgccccg cgccgtccgc actctggccg ccgctttcgc cgactacccc
     3721 gctacccggc acaccgtgga ccccgaccgg cacatcgagc gtgtgacaga gttgcaggag
     3781 ctgttcctga cccgcgtcgg gctggacatc ggcaaggtgt gggtagccga cgacggcgcg
     3841 gccgtggccg tgtggactac ccccgagagc gttgaggccg gcgccgtgtt cgccgagatc
     3901 ggcccccgaa tggccgagct gagcggcagc cgcctggccg cccagcagca aatggagggc
     3961 ctgcttgccc cccatcgtcc caaggagcct gcctggtttc tggccactgt aggagtgagc
     4021 cccgaccacc agggcaaggg cttgggcagc gccgtcgtgt tgcccggcgt agaggccgcc
     4081 gaacgcgccg gtgtgcccgc ctttctcgaa acaagcgcac caagaaacct tccattctac
     4141 gagcgcctgg gcttcaccgt gaccgccgat gtcgaggtgc ccgagggacc taggacctgg
     4201 tgtatgacac gaaaacctgg cgcctaagcg gccgcgatcc agacatgata agatacattg
     4261 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     4321 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     4381 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     4441 aaaacctcta caaatggctg atcccaatgg cgcgccgagc ttggcgtaat catggtcata
     4501 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     4561 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     4621 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     4681 acgcgcgggg agaggcggtt tgcgtattgg gcgctgttcc gcttcctcgc tcactgactc
     4741 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     4801 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4861 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4921 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4981 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     5041 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     5101 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     5161 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5221 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5281 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     5341 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5401 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5461 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5521 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     5581 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     5641 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     5701 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     5761 cttaccatct ggccccagtg ctgcaatgat accgcgagaa ccacgctcac cggctccaga
     5821 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     5881 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     5941 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     6001 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     6061 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     6121 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     6181 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     6241 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     6301 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     6361 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6421 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     6481 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     6541 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     6601 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     6661 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct tttgtc
//
