LOCUS Exported 7088 bp ds-DNA circular SYN 01-DEC-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7088) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Dec 10, 2020 from SnapGene Viewer 5.2.3 https://www.snapgene.com FEATURES Location/Qualifiers source 1..7088 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 56..222 /label=ITR /note="ITR" misc_feature 239..244 /label=BglII /note="BglII" misc_feature 245..716 /label=synapsin promoter /note="synapsin promoter" CDS 748..750 /codon_start=1 /label=Start /note="IgG K-leader" /translation="M" CDS 751..1725 /codon_start=1 /label=TtGBP 2-326 /note="TtGBP 1-326" /translation="KLEIFSWWAGDEGPALEALIRLYKQKYPGVEVINATVTGGAGVNA RAVLKTRMLGGDPPDTFQVAAGMELIGTWVVANRMEDLSALFRQEGWLQAFPKGLIDLI SYKGGIWSVPVNIHRSNVMWYLPAKLKEWGVNPPRTWDEFLATCQTLKQKGLEAPLALG ENWTQQHLWESVALAVLGPDDWNNLWNGKLKFTDPKAVRAWEVFGRVLDCANKDAAGLS WQQAVDRVVQGKAAFNVMGDWAAGYMTTTLKLKPGTDFAWAPSPGTQGVFMMVSDSFGL PKGAKNRQNAINWLRLVGSKEGQDTFNPLKGSIAARLDSDPSKY" misc_feature 943..945 /label=H66A /note="H66A" misc_feature 1573..1575 /label=L276V (binding site) CDS 1726..1731 /codon_start=1 /label=+PA /translation="PA" CDS 1732..2007 /codon_start=1 /label=cpGFP148-238 /note="cpGFP148-238" /translation="SHNVYIMADKQRNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDG PVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK" misc_feature 1765..1767 /label=K158R (historical arefact) CDS 2008..2025 /codon_start=1 /label=Linker /note="Linker" /translation="GGTGGS" CDS 2026..2466 /codon_start=1 /label=cpGFP 1-146 /note="cpGFP 1-146" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFN" CDS 2467..2472 /codon_start=1 /label=+NP /translation="NP" CDS 2473..2676 /codon_start=1 /label=TtGBP 327-392 /note="TtGBP 329-392" /translation="NAYGQSAMRDWRSNRIVGSLVAGAVAPESFMSQFGTVMEIFLQTR NPQAAANAAQAIADQVGLGRLGQ" misc_feature 2536..2538 /label=H348A /note="H348A" CDS 2677..2688 /codon_start=1 /label=MCS /translation="LQVD" CDS 2689..2718 /codon_start=1 /label=myc tag /note="myc tag" /translation="EQKLISEEDL" CDS 2719..2748 /codon_start=1 /label=linker /note="linker" /translation="NAVGQDTQER" CDS 2749..3459 /codon_start=1 /label=mRuby2 /note="mRuby2" /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV AKFAGLGGGMDELYK" misc_feature 3475..4063 /label=WPRE /note="WPRE" misc_feature 3611..3631 /label=Mao 115r /note="Mao 115r" misc_feature 4097..4328 /label=SV40 /note="SV40" misc_feature 4357..4523 /label=ITR /note="ITR" ORIGIN 1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgcgc 61 gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc 121 ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt 181 tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg ctctaggaag 241 atctctgcag agggccctgc gtatgagtgc aagtgggttt taggaccagg atgaggcggg 301 gtgggggtgc ctacctgacg accgaccccg acccactgga caagcaccca acccccattc 361 cccaaattgc gcatccccta tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt 421 gcgcactgcc agcttcagca ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc 481 accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg caaactcccc 541 ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg caccacgcga 601 ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc gactcagcgc 661 tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg cagtccaatt 721 caagctgcta gcaaggatcc cgccaccatg aaactggaaa tttttagctg gtgggccggc 781 gatgaaggcc cggccctgga agccctgatt cgcctgtata aacagaaata tccgggcgtg 841 gaggtgatta acgccaccgt gaccggcggc gccggcgtga acgcccgcgc cgtgctgaaa 901 acccgcatgc tgggcggcga tccgccggat acctttcagg tggcagccgg catggaactg 961 attggcacct gggtggtggc caaccgcatg gaagacctga gcgccctgtt tcgccaggaa 1021 ggctggctgc aagcctttcc gaaaggcctg attgatctga ttagctataa aggcggcatt 1081 tggagcgtgc cggtgaacat tcatcgcagc aacgtgatgt ggtatctgcc ggccaaactg 1141 aaagaatggg gcgtgaaccc gccgcgcacc tgggatgaat ttctggccac ctgccagacc 1201 ctgaaacaga aaggcctgga agccccgctg gccctgggcg aaaactggac ccagcagcat 1261 ctgtgggaaa gcgtggccct ggctgtgctg ggcccggatg attggaacaa cctgtggaac 1321 ggcaaactga aatttaccga tccgaaagcc gtgcgcgcct gggaagtgtt tggccgcgtg 1381 ctggattgcg ccaacaaaga tgccgccggc ctgagctggc agcaggccgt ggatcgcgtg 1441 gtgcagggca aagccgcctt taacgtgatg ggcgattggg ccgccggcta tatgaccacc 1501 accctgaaac tgaaaccggg caccgatttt gcctgggccc cgagcccggg cacccagggc 1561 gtgtttatga tggtgagcga tagctttggc ctgccgaaag gcgccaaaaa ccgccagaac 1621 gccattaact ggctgcgcct ggtgggcagc aaagaaggcc aggatacctt taacccgctg 1681 aaaggcagca ttgccgcccg cctggatagc gatccgagca aatatcccgc cagccacaac 1741 gtctatatca tggccgacaa gcagaggaac ggcatcaagg cgaacttcaa gatccgccac 1801 aacatcgagg acggcggcgt gcagctcgcc taccactacc agcagaacac ccccatcggc 1861 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa actttcgaaa 1921 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 1981 actctcggca tggacgagct gtacaagggc ggtaccggag ggagcatggt gagcaagggc 2041 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 2101 cacaagttca gcgtgtccgg cgagggtgag ggcgatgcca cctacggcaa gctgaccctg 2161 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 2221 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc 2281 aagtccgcca tgcccgaagg ctacatccag gagcgcacca tcttcttcaa ggacgacggc 2341 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 2401 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcataagct ggagtacaac 2461 tttaacaacc ccaacgccta tggccagagc gccatgcgcg attggcgcag caaccgcatt 2521 gtgggcagcc tggtggcagg cgccgtggcc ccggaaagct ttatgagcca gtttggcacc 2581 gtgatggaaa tttttctgca aacccgcaac ccgcaggccg ccgccaacgc cgcccaggcc 2641 attgccgatc aggtgggcct gggccgcctg ggccagctgc aggtcgacga acaaaaactc 2701 atctcagaag aggatctgaa tgctgtgggc caggacacgc aggagcgtat ggtgtctaag 2761 ggcgaagagc tgatcaagga aaatatgcgt atgaaggtgg tcatggaagg ttcggtcaac 2821 ggccaccaat tcaaatgcac aggtgaagga gaaggcaatc cgtacatggg aactcaaacc 2881 atgaggatca aagtcatcga gggaggaccc ctgccatttg cctttgacat tcttgccacg 2941 tcgttcatgt atggcagccg tacttttatc aagtacccga aaggcattcc tgatttcttt 3001 aaacagtcct ttcctgaggg ttttacttgg gaaagagtta cgagatacga agatggtgga 3061 gtcgtcaccg tcatgcagga caccagcctt gaggatggct gtctcgttta ccacgtccaa 3121 gtcagagggg taaactttcc ttccaatggt cccgtgatgc agaagaagac caagggttgg 3181 gagcctaata cagagatgat gtatccagca gatggtggtc tgaggggata cactcatatg 3241 gcactgaaag ttgatggtgg tggccatctg tcttgctctt tcgtaacaac ttacaggtca 3301 aaaaagaccg tcgggaacat caagatgccc ggtatccatg ccgttgatca ccgcctggaa 3361 aggttagagg aaagtgacaa tgaaatgttc gtagtacaac gcgaacacgc agttgccaag 3421 ttcgccgggc ttggtggtgg gatggacgag ctgtacaagt agaagcttat cgataatcaa 3481 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 3541 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 3601 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 3661 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 3721 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 3781 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 3841 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt 3901 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 3961 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 4021 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct 4081 cgacccgggc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc 4141 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta 4201 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg 4261 tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt 4321 ggtaaaatcg ataaggatct tcctagagca tggctacgta gataagtagc atggcgggtt 4381 aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg 4441 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc 4501 ctcagtgagc gagcgagcgc gcagccttaa ttaacctaat tcactggccg tcgttttaca 4561 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 4621 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 4681 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 4741 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 4801 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 4861 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 4921 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 4981 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 5041 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 5101 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt 5161 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 5221 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 5281 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 5341 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 5401 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 5461 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 5521 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 5581 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 5641 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 5701 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 5761 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 5821 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 5881 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 5941 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 6001 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 6061 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 6121 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 6181 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 6241 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 6301 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 6361 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 6421 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 6481 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 6541 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 6601 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 6661 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 6721 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 6781 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 6841 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 6901 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 6961 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 7021 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 7081 gcggaaga //