LOCUS       Exported                7088 bp ds-DNA     circular SYN 01-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 10, 2020 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    56..222
                     /label=ITR
                     /note="ITR"
     misc_feature    239..244
                     /label=BglII
                     /note="BglII"
     misc_feature    245..716
                     /label=synapsin promoter
                     /note="synapsin promoter"
     CDS             748..750
                     /codon_start=1
                     /label=Start
                     /note="IgG K-leader"
                     /translation="M"
     CDS             751..1725
                     /codon_start=1
                     /label=TtGBP 2-326
                     /note="TtGBP 1-326"
                     /translation="KLEIFSWWAGDEGPALEALIRLYKQKYPGVEVINATVTGGAGVNA
                     RAVLKTRMLGGDPPDTFQVAAGMELIGTWVVANRMEDLSALFRQEGWLQAFPKGLIDLI
                     SYKGGIWSVPVNIHRSNVMWYLPAKLKEWGVNPPRTWDEFLATCQTLKQKGLEAPLALG
                     ENWTQQHLWESVALAVLGPDDWNNLWNGKLKFTDPKAVRAWEVFGRVLDCANKDAAGLS
                     WQQAVDRVVQGKAAFNVMGDWAAGYMTTTLKLKPGTDFAWAPSPGTQGVFMMVSDSFGL
                     PKGAKNRQNAINWLRLVGSKEGQDTFNPLKGSIAARLDSDPSKY"
     misc_feature    943..945
                     /label=H66A
                     /note="H66A"
     misc_feature    1573..1575
                     /label=L276V (binding site)
     CDS             1726..1731
                     /codon_start=1
                     /label=+PA
                     /translation="PA"
     CDS             1732..2007
                     /codon_start=1
                     /label=cpGFP148-238
                     /note="cpGFP148-238"
                     /translation="SHNVYIMADKQRNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDG
                     PVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    1765..1767
                     /label=K158R (historical arefact)
     CDS             2008..2025
                     /codon_start=1
                     /label=Linker
                     /note="Linker"
                     /translation="GGTGGS"
     CDS             2026..2466
                     /codon_start=1
                     /label=cpGFP 1-146
                     /note="cpGFP 1-146"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFN"
     CDS             2467..2472
                     /codon_start=1
                     /label=+NP
                     /translation="NP"
     CDS             2473..2676
                     /codon_start=1
                     /label=TtGBP 327-392
                     /note="TtGBP 329-392"
                     /translation="NAYGQSAMRDWRSNRIVGSLVAGAVAPESFMSQFGTVMEIFLQTR
                     NPQAAANAAQAIADQVGLGRLGQ"
     misc_feature    2536..2538
                     /label=H348A
                     /note="H348A"
     CDS             2677..2688
                     /codon_start=1
                     /label=MCS
                     /translation="LQVD"
     CDS             2689..2718
                     /codon_start=1
                     /label=myc tag
                     /note="myc tag"
                     /translation="EQKLISEEDL"
     CDS             2719..2748
                     /codon_start=1
                     /label=linker
                     /note="linker"
                     /translation="NAVGQDTQER"
     CDS             2749..3459
                     /codon_start=1
                     /label=mRuby2
                     /note="mRuby2"
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     misc_feature    3475..4063
                     /label=WPRE
                     /note="WPRE"
     misc_feature    3611..3631
                     /label=Mao 115r
                     /note="Mao 115r"
     misc_feature    4097..4328
                     /label=SV40
                     /note="SV40"
     misc_feature    4357..4523
                     /label=ITR
                     /note="ITR"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgcgc
       61 gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc
      121 ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt
      181 tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg ctctaggaag
      241 atctctgcag agggccctgc gtatgagtgc aagtgggttt taggaccagg atgaggcggg
      301 gtgggggtgc ctacctgacg accgaccccg acccactgga caagcaccca acccccattc
      361 cccaaattgc gcatccccta tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt
      421 gcgcactgcc agcttcagca ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc
      481 accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg caaactcccc
      541 ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg caccacgcga
      601 ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc gactcagcgc
      661 tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg cagtccaatt
      721 caagctgcta gcaaggatcc cgccaccatg aaactggaaa tttttagctg gtgggccggc
      781 gatgaaggcc cggccctgga agccctgatt cgcctgtata aacagaaata tccgggcgtg
      841 gaggtgatta acgccaccgt gaccggcggc gccggcgtga acgcccgcgc cgtgctgaaa
      901 acccgcatgc tgggcggcga tccgccggat acctttcagg tggcagccgg catggaactg
      961 attggcacct gggtggtggc caaccgcatg gaagacctga gcgccctgtt tcgccaggaa
     1021 ggctggctgc aagcctttcc gaaaggcctg attgatctga ttagctataa aggcggcatt
     1081 tggagcgtgc cggtgaacat tcatcgcagc aacgtgatgt ggtatctgcc ggccaaactg
     1141 aaagaatggg gcgtgaaccc gccgcgcacc tgggatgaat ttctggccac ctgccagacc
     1201 ctgaaacaga aaggcctgga agccccgctg gccctgggcg aaaactggac ccagcagcat
     1261 ctgtgggaaa gcgtggccct ggctgtgctg ggcccggatg attggaacaa cctgtggaac
     1321 ggcaaactga aatttaccga tccgaaagcc gtgcgcgcct gggaagtgtt tggccgcgtg
     1381 ctggattgcg ccaacaaaga tgccgccggc ctgagctggc agcaggccgt ggatcgcgtg
     1441 gtgcagggca aagccgcctt taacgtgatg ggcgattggg ccgccggcta tatgaccacc
     1501 accctgaaac tgaaaccggg caccgatttt gcctgggccc cgagcccggg cacccagggc
     1561 gtgtttatga tggtgagcga tagctttggc ctgccgaaag gcgccaaaaa ccgccagaac
     1621 gccattaact ggctgcgcct ggtgggcagc aaagaaggcc aggatacctt taacccgctg
     1681 aaaggcagca ttgccgcccg cctggatagc gatccgagca aatatcccgc cagccacaac
     1741 gtctatatca tggccgacaa gcagaggaac ggcatcaagg cgaacttcaa gatccgccac
     1801 aacatcgagg acggcggcgt gcagctcgcc taccactacc agcagaacac ccccatcggc
     1861 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa actttcgaaa
     1921 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1981 actctcggca tggacgagct gtacaagggc ggtaccggag ggagcatggt gagcaagggc
     2041 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     2101 cacaagttca gcgtgtccgg cgagggtgag ggcgatgcca cctacggcaa gctgaccctg
     2161 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     2221 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     2281 aagtccgcca tgcccgaagg ctacatccag gagcgcacca tcttcttcaa ggacgacggc
     2341 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     2401 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcataagct ggagtacaac
     2461 tttaacaacc ccaacgccta tggccagagc gccatgcgcg attggcgcag caaccgcatt
     2521 gtgggcagcc tggtggcagg cgccgtggcc ccggaaagct ttatgagcca gtttggcacc
     2581 gtgatggaaa tttttctgca aacccgcaac ccgcaggccg ccgccaacgc cgcccaggcc
     2641 attgccgatc aggtgggcct gggccgcctg ggccagctgc aggtcgacga acaaaaactc
     2701 atctcagaag aggatctgaa tgctgtgggc caggacacgc aggagcgtat ggtgtctaag
     2761 ggcgaagagc tgatcaagga aaatatgcgt atgaaggtgg tcatggaagg ttcggtcaac
     2821 ggccaccaat tcaaatgcac aggtgaagga gaaggcaatc cgtacatggg aactcaaacc
     2881 atgaggatca aagtcatcga gggaggaccc ctgccatttg cctttgacat tcttgccacg
     2941 tcgttcatgt atggcagccg tacttttatc aagtacccga aaggcattcc tgatttcttt
     3001 aaacagtcct ttcctgaggg ttttacttgg gaaagagtta cgagatacga agatggtgga
     3061 gtcgtcaccg tcatgcagga caccagcctt gaggatggct gtctcgttta ccacgtccaa
     3121 gtcagagggg taaactttcc ttccaatggt cccgtgatgc agaagaagac caagggttgg
     3181 gagcctaata cagagatgat gtatccagca gatggtggtc tgaggggata cactcatatg
     3241 gcactgaaag ttgatggtgg tggccatctg tcttgctctt tcgtaacaac ttacaggtca
     3301 aaaaagaccg tcgggaacat caagatgccc ggtatccatg ccgttgatca ccgcctggaa
     3361 aggttagagg aaagtgacaa tgaaatgttc gtagtacaac gcgaacacgc agttgccaag
     3421 ttcgccgggc ttggtggtgg gatggacgag ctgtacaagt agaagcttat cgataatcaa
     3481 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3541 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3601 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3661 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3721 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3781 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3841 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3901 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3961 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4021 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     4081 cgacccgggc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
     4141 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     4201 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     4261 tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     4321 ggtaaaatcg ataaggatct tcctagagca tggctacgta gataagtagc atggcgggtt
     4381 aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     4441 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     4501 ctcagtgagc gagcgagcgc gcagccttaa ttaacctaat tcactggccg tcgttttaca
     4561 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     4621 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     4681 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4741 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4801 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4861 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     4921 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4981 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     5041 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     5101 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     5161 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5221 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5281 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5341 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5401 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5461 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5521 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5581 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5641 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5701 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5761 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5821 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5881 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5941 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6001 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6061 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6121 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6181 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6241 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6301 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6361 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6421 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6481 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6541 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6601 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6661 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6721 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6781 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6841 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6901 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6961 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7021 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7081 gcggaaga
//
