LOCUS       Exported                6499 bp ds-DNA     circular SYN 01-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 10, 2020 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    57..223
                     /label=ITR
                     /note="ITR"
     misc_feature    240..245
                     /label=BglII
                     /note="BglII"
     misc_feature    246..935
                     /label=GFAP promoter
                     /note="GFAP promoter"
     CDS             943..945
                     /codon_start=1
                     /label=Start
                     /note="IgG K-leader"
                     /translation="M"
     CDS             946..1920
                     /codon_start=1
                     /label=TtGBP 2-326
                     /note="TtGBP 1-326"
                     /translation="KLEIFSWWAGDEGPALEALIRLYKQKYPGVEVINATVTGGAGVNA
                     RAVLKTRMLGGDPPDTFQVAAGMELIGTWVVANRMEDLSALFRQEGWLQAFPKGLIDLI
                     SYKGGIWSVPVNIHRSNVMWYLPAKLKEWGVNPPRTWDEFLATCQTLKQKGLEAPLALG
                     ENWTQQHLWESVALAVLGPDDWNNLWNGKLKFTDPKAVRAWEVFGRVLDCANKDAAGLS
                     WQQAVDRVVQGKAAFNVMGDWAAGYMTTTLKLKPGTDFAWAPSPGTQGVFMMVSDSFGL
                     PKGAKNRQNAINWLRLVGSKEGQDTFNPLKGSIAARLDSDPSKY"
     misc_feature    1138..1140
                     /label=H66A
                     /note="H66A"
     misc_feature    1768..1770
                     /label=L276V (binding site)
     CDS             1921..1926
                     /codon_start=1
                     /label=+PA
                     /translation="PA"
     CDS             1927..2202
                     /codon_start=1
                     /label=cpGFP148-238
                     /note="cpGFP148-238"
                     /translation="SHNVYIMADKQRNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDG
                     PVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    1960..1962
                     /label=K158R (historical arefact)
     CDS             2203..2220
                     /codon_start=1
                     /label=Linker
                     /note="Linker"
                     /translation="GGTGGS"
     CDS             2221..2661
                     /codon_start=1
                     /label=cpGFP 1-146
                     /note="cpGFP 1-146"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFN"
     CDS             2662..2667
                     /codon_start=1
                     /label=+NP
                     /translation="NP"
     CDS             2668..2871
                     /codon_start=1
                     /label=TtGBP 327-392
                     /note="TtGBP 329-392"
                     /translation="NAYGQSAMRDWRSNRIVGSLVAGAVAPESFMSQFGTVMEIFLQTR
                     NPQAAANAAQAIADQVGLGRLGQ"
     misc_feature    2731..2733
                     /label=H348A
                     /note="H348A"
     misc_feature    2887..3475
                     /label=WPRE
                     /note="WPRE"
     misc_feature    3023..3043
                     /label=Mao 115r
                     /note="Mao 115r"
     misc_feature    3509..3740
                     /label=SV40
                     /note="SV40"
     misc_feature    3769..3935
                     /label=ITR
                     /note="ITR"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg
       61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg
      121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
      181 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa
      241 gatctaacat atcctggtgt ggagtagggg acgctgctct gacagaggct cgggggcctg
      301 agctggctct gtgagctggg gaggaggcag acagccaggc ctttgtctgc aagcagacct
      361 ggcagcattg ggctggccgc cccccagggc ctcctcttca tgcccagtga atgactcacc
      421 ttggcacaga cacaatgttc ggggtgggca cagtgcctgc ttcccgccgc accccagccc
      481 ccctcaaatg ccttccgaga agcccattga gcagggggct tgcattgcac cccagcctga
      541 cagcctggca tcttgggata aaagcagcac agccccctag gggctgccct tgctgtgtgg
      601 cgccaccggc ggtggagaac aaggctctat tcagcctgtg cccaggaaag gggatcaggg
      661 gatgcccagg catggacagt gggtggcagg gggggagagg agggctgtct gcttcccaga
      721 agtccaagga cacaaatggg tgaggggaga gctctcccca tagctgggct gcggcccaac
      781 cccaccccct caggctatgc cagggggtgt tgccaggggc acccgggcat cgccagtcta
      841 gcccactcct tcataaagcc ctcgcatccc aggagcgagc agagccagag caggttggag
      901 aggagacgca tcacctccgc tgctcgcggg gatcccgcca ccatgaaact ggaaattttt
      961 agctggtggg ccggcgatga aggcccggcc ctggaagccc tgattcgcct gtataaacag
     1021 aaatatccgg gcgtggaggt gattaacgcc accgtgaccg gcggcgccgg cgtgaacgcc
     1081 cgcgccgtgc tgaaaacccg catgctgggc ggcgatccgc cggatacctt tcaggtggca
     1141 gccggcatgg aactgattgg cacctgggtg gtggccaacc gcatggaaga cctgagcgcc
     1201 ctgtttcgcc aggaaggctg gctgcaagcc tttccgaaag gcctgattga tctgattagc
     1261 tataaaggcg gcatttggag cgtgccggtg aacattcatc gcagcaacgt gatgtggtat
     1321 ctgccggcca aactgaaaga atggggcgtg aacccgccgc gcacctggga tgaatttctg
     1381 gccacctgcc agaccctgaa acagaaaggc ctggaagccc cgctggccct gggcgaaaac
     1441 tggacccagc agcatctgtg ggaaagcgtg gccctggctg tgctgggccc ggatgattgg
     1501 aacaacctgt ggaacggcaa actgaaattt accgatccga aagccgtgcg cgcctgggaa
     1561 gtgtttggcc gcgtgctgga ttgcgccaac aaagatgccg ccggcctgag ctggcagcag
     1621 gccgtggatc gcgtggtgca gggcaaagcc gcctttaacg tgatgggcga ttgggccgcc
     1681 ggctatatga ccaccaccct gaaactgaaa ccgggcaccg attttgcctg ggccccgagc
     1741 ccgggcaccc agggcgtgtt tatgatggtg agcgatagct ttggcctgcc gaaaggcgcc
     1801 aaaaaccgcc agaacgccat taactggctg cgcctggtgg gcagcaaaga aggccaggat
     1861 acctttaacc cgctgaaagg cagcattgcc gcccgcctgg atagcgatcc gagcaaatat
     1921 cccgccagcc acaacgtcta tatcatggcc gacaagcaga ggaacggcat caaggcgaac
     1981 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag
     2041 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     2101 tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2161 accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc
     2221 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     2281 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccacctac
     2341 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     2401 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     2461 cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc
     2521 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     2581 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcat
     2641 aagctggagt acaactttaa caaccccaac gcctatggcc agagcgccat gcgcgattgg
     2701 cgcagcaacc gcattgtggg cagcctggtg gcaggcgccg tggccccgga gagctttatg
     2761 agccagtttg gcaccgtgat ggaaattttt ctgcaaaccc gcaacccgca ggccgccgcc
     2821 aacgccgccc aggccattgc cgatcaggtg ggcctgggcc gcctgggcca gtagaagctt
     2881 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2941 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3001 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3061 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3121 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3181 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3241 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     3301 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3361 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3421 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     3481 taccgtcgac ctcgacccgg gcggccgctt cgagcagaca tgataagata cattgatgag
     3541 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     3601 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     3661 attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag caagtaaaac
     3721 ctctacaaat gtggtaaaat cgataaggat cttcctagag catggctacg tagataagta
     3781 gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg ccactccctc
     3841 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
     3901 tgcccgggcg gcctcagtga gcgagcgagc gcgcagcctt aattaaccta attcactggc
     3961 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     4021 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     4081 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     4141 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4201 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4261 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4321 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4381 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4441 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4501 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     4561 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4621 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4681 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     4741 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     4801 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     4861 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     4921 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     4981 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5041 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5101 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5161 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5221 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5281 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5341 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5401 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5461 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     5521 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     5581 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5641 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5701 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5761 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5821 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     5881 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5941 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6001 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6061 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6121 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6181 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6241 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6301 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6361 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6421 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6481 gtgagcgagg aagcggaag
//
