LOCUS       Exported                7282 bp ds-DNA     circular SYN 01-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 10, 2020 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    57..223
                     /label=ITR
                     /note="ITR"
     misc_feature    240..245
                     /label=BglII
                     /note="BglII"
     misc_feature    246..935
                     /label=GFAP promoter
                     /note="GFAP promoter"
     CDS             943..945
                     /codon_start=1
                     /label=Start
                     /note="IgG K-leader"
                     /translation="M"
     CDS             946..1920
                     /codon_start=1
                     /label=TtGBP 2-326
                     /note="TtGBP 1-326"
                     /translation="KLEIFSWWAGDEGPALEALIRLYKQKYPGVEVINATVTGGAGVNA
                     RAVLKTRMLGGDPPDTFQVAAGMELIGTWVVANRMEDLSALFRQEGWLQAFPKGLIDLI
                     SYKGGIWSVPVNIHRSNVMWYLPAKLKEWGVNPPRTWDEFLATCQTLKQKGLEAPLALG
                     ENWTQQHLWESVALAVLGPDDWNNLWNGKLKFTDPKAVRAWEVFGRVLDCANKDAAGLS
                     WQQAVDRVVQGKAAFNVMGDWAAGYMTTTLKLKPGTDFAWAPSPGTQGVFMMVSDSFGL
                     PKGAKNRQNAINWLRLVGSKEGQDTFNPLKGSIAARLDSDPSKY"
     misc_feature    1138..1140
                     /label=H66A
                     /note="H66A"
     misc_feature    1768..1770
                     /label=L276V (binding site)
     CDS             1921..1926
                     /codon_start=1
                     /label=+PA
                     /translation="PA"
     CDS             1927..2202
                     /codon_start=1
                     /label=cpGFP148-238
                     /note="cpGFP148-238"
                     /translation="SHNVYIMADKQRNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDG
                     PVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    1960..1962
                     /label=K158R (historical arefact)
     CDS             2203..2220
                     /codon_start=1
                     /label=Linker
                     /note="Linker"
                     /translation="GGTGGS"
     CDS             2221..2661
                     /codon_start=1
                     /label=cpGFP 1-146
                     /note="cpGFP 1-146"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFN"
     CDS             2662..2667
                     /codon_start=1
                     /label=+NP
                     /translation="NP"
     CDS             2668..2871
                     /codon_start=1
                     /label=TtGBP 327-392
                     /note="TtGBP 329-392"
                     /translation="NAYGQSAMRDWRSNRIVGSLVAGAVAPESFMSQFGTVMEIFLQTR
                     NPQAAANAAQAIADQVGLGRLGQ"
     misc_feature    2731..2733
                     /label=H348A
                     /note="H348A"
     CDS             2872..2883
                     /codon_start=1
                     /label=MCS
                     /translation="LQVD"
     CDS             2884..2913
                     /codon_start=1
                     /label=myc tag
                     /note="myc tag"
                     /translation="EQKLISEEDL"
     CDS             2914..2943
                     /codon_start=1
                     /label=linker
                     /note="linker"
                     /translation="NAVGQDTQER"
     CDS             2944..3654
                     /codon_start=1
                     /label=mRuby2
                     /note="mRuby2"
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     misc_feature    3670..4258
                     /label=WPRE
                     /note="WPRE"
     misc_feature    3806..3826
                     /label=Mao 115r
                     /note="Mao 115r"
     misc_feature    4292..4523
                     /label=SV40
                     /note="SV40"
     misc_feature    4552..4718
                     /label=ITR
                     /note="ITR"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg
       61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg
      121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
      181 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa
      241 gatctaacat atcctggtgt ggagtagggg acgctgctct gacagaggct cgggggcctg
      301 agctggctct gtgagctggg gaggaggcag acagccaggc ctttgtctgc aagcagacct
      361 ggcagcattg ggctggccgc cccccagggc ctcctcttca tgcccagtga atgactcacc
      421 ttggcacaga cacaatgttc ggggtgggca cagtgcctgc ttcccgccgc accccagccc
      481 ccctcaaatg ccttccgaga agcccattga gcagggggct tgcattgcac cccagcctga
      541 cagcctggca tcttgggata aaagcagcac agccccctag gggctgccct tgctgtgtgg
      601 cgccaccggc ggtggagaac aaggctctat tcagcctgtg cccaggaaag gggatcaggg
      661 gatgcccagg catggacagt gggtggcagg gggggagagg agggctgtct gcttcccaga
      721 agtccaagga cacaaatggg tgaggggaga gctctcccca tagctgggct gcggcccaac
      781 cccaccccct caggctatgc cagggggtgt tgccaggggc acccgggcat cgccagtcta
      841 gcccactcct tcataaagcc ctcgcatccc aggagcgagc agagccagag caggttggag
      901 aggagacgca tcacctccgc tgctcgcggg gatcccgcca ccatgaaact ggaaattttt
      961 agctggtggg ccggcgatga aggcccggcc ctggaagccc tgattcgcct gtataaacag
     1021 aaatatccgg gcgtggaggt gattaacgcc accgtgaccg gcggcgccgg cgtgaacgcc
     1081 cgcgccgtgc tgaaaacccg catgctgggc ggcgatccgc cggatacctt tcaggtggca
     1141 gccggcatgg aactgattgg cacctgggtg gtggccaacc gcatggaaga cctgagcgcc
     1201 ctgtttcgcc aggaaggctg gctgcaagcc tttccgaaag gcctgattga tctgattagc
     1261 tataaaggcg gcatttggag cgtgccggtg aacattcatc gcagcaacgt gatgtggtat
     1321 ctgccggcca aactgaaaga atggggcgtg aacccgccgc gcacctggga tgaatttctg
     1381 gccacctgcc agaccctgaa acagaaaggc ctggaagccc cgctggccct gggcgaaaac
     1441 tggacccagc agcatctgtg ggaaagcgtg gccctggctg tgctgggccc ggatgattgg
     1501 aacaacctgt ggaacggcaa actgaaattt accgatccga aagccgtgcg cgcctgggaa
     1561 gtgtttggcc gcgtgctgga ttgcgccaac aaagatgccg ccggcctgag ctggcagcag
     1621 gccgtggatc gcgtggtgca gggcaaagcc gcctttaacg tgatgggcga ttgggccgcc
     1681 ggctatatga ccaccaccct gaaactgaaa ccgggcaccg attttgcctg ggccccgagc
     1741 ccgggcaccc agggcgtgtt tatgatggtg agcgatagct ttggcctgcc gaaaggcgcc
     1801 aaaaaccgcc agaacgccat taactggctg cgcctggtgg gcagcaaaga aggccaggat
     1861 acctttaacc cgctgaaagg cagcattgcc gcccgcctgg atagcgatcc gagcaaatat
     1921 cccgccagcc acaacgtcta tatcatggcc gacaagcaga ggaacggcat caaggcgaac
     1981 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag
     2041 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     2101 tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2161 accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc
     2221 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     2281 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccacctac
     2341 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     2401 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     2461 cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc
     2521 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     2581 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcat
     2641 aagctggagt acaactttaa caaccccaac gcctatggcc agagcgccat gcgcgattgg
     2701 cgcagcaacc gcattgtggg cagcctggtg gcaggcgccg tggccccgga gagctttatg
     2761 agccagtttg gcaccgtgat ggaaattttt ctgcaaaccc gcaacccgca ggccgccgcc
     2821 aacgccgccc aggccattgc cgatcaggtg ggcctgggcc gcctgggcca actgcaggtc
     2881 gacgaacaaa aactcatctc agaagaggat ctgaatgctg tgggccagga cacgcaggag
     2941 cgtatggtgt ctaagggcga agagctgatc aaggaaaata tgcgtatgaa ggtggtcatg
     3001 gaaggttcgg tcaacggcca ccaattcaaa tgcacaggtg aaggagaagg caatccgtac
     3061 atgggaactc aaaccatgag gatcaaagtc atcgagggag gacccctgcc atttgccttt
     3121 gacattcttg ccacgtcgtt catgtatggc agccgtactt ttatcaagta cccgaaaggc
     3181 attcctgatt tctttaaaca gtcctttcct gagggtttta cttgggaaag agttacgaga
     3241 tacgaagatg gtggagtcgt caccgtcatg caggacacca gccttgagga tggctgtctc
     3301 gtttaccacg tccaagtcag aggggtaaac tttccttcca atggtcccgt gatgcagaag
     3361 aagaccaagg gttgggagcc taatacagag atgatgtatc cagcagatgg tggtctgagg
     3421 ggatacactc atatggcact gaaagttgat ggtggtggcc atctgtcttg ctctttcgta
     3481 acaacttaca ggtcaaaaaa gaccgtcggg aacatcaaga tgcccggtat ccatgccgtt
     3541 gatcaccgcc tggaaaggtt agaggaaagt gacaatgaaa tgttcgtagt acaacgcgaa
     3601 cacgcagttg ccaagttcgc cgggcttggt ggtgggatgg acgagctgta caagtagaag
     3661 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3721 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3781 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3841 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3901 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3961 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4021 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     4081 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4141 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4201 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     4261 cgataccgtc gacctcgacc cgggcggccg cttcgagcag acatgataag atacattgat
     4321 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     4381 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     4441 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa
     4501 aacctctaca aatgtggtaa aatcgataag gatcttccta gagcatggct acgtagataa
     4561 gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc
     4621 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     4681 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc cttaattaac ctaattcact
     4741 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     4801 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     4861 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     4921 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4981 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     5041 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     5101 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     5161 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     5221 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     5281 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     5341 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5401 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5461 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5521 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5581 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5641 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5701 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     5761 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5821 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5881 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5941 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6001 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6061 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6121 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6181 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6241 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6301 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6361 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6421 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6481 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6541 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6601 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6661 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6721 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6781 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6841 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6901 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6961 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7021 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7081 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7141 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7201 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     7261 tcagtgagcg aggaagcgga ag
//
