LOCUS       PB-TAG-Halo-ERN_Destin 13475 bp ds-DNA     circular     12-JUL-2018
DEFINITION  .
KEYWORDS    "marker:AmpR, CmR, NeoR/KanR" "ref:pmid\:26025620"                 
FEATURES             Location/Qualifiers
     repeat_region   711..773
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein_bind    1219..1237
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     CDS             8735..8743
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             8660..8695
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     protein_bind    11347..11363
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     CDS             2489..2509
                     /label="TEV Protease CDS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             8621..8656
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     enhancer        6180..6559
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     603..619
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     promoter        complement(11371..11377)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     polyA_signal    10154..10726
                     /label="EF-1α poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     polyA_site      5684..5739
                     /label="β-globin poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        9247..9293
                     /label="EM7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    complement(4135..4259)
                     /label="attR2"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    1293..1311
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    4876..4878
                     /label="IRES2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2534..2658
                     /label="attR1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    1367..1385
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    complement(1939..1958)
                     /label="Halo-RTqPCR2R"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(13276..13344)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     complement(11323..11339)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     rep_origin      complement(11725..12313)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     repeat_region   11100..11134
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        complement(11396..11401)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             1586..2476
                     /label="Halo Tag 7 CDS"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1808..1829
                     /label="Halo-RTqPCR2F"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             7997..8614
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             4894..5607
                     /label="EGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             4891..4893
                     /label="EGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    1404..1422
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(11378..11395)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    11416..11437
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        626..644
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        9285..9303
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(12484..13275)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             2767..3426
                     /label="Chlor. Resistance"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        complement(13345..13449)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    1145..1163
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     CDS             3789..4094
                     /label="ccdB"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    4879..4887
                     /label="IRES2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    1330..1348
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     CDS             9313..10107
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      complement(3..458)
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(11284..11302)
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    8770..9214
                     /label="FMDV IRES"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(3419..3438)
                     /label="180509_Rev_Anneal"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    1182..1200
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    4301..4875
                     /label="IRES2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1571..1588
                     /label="180509_Forward Halo Anneal"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             8615..8620
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             8657..8659
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             4888..4890
                     /label="EGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             8699..8734
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     protein_bind    1256..1274
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     CDS             8696..8698
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1578..1585
                     /label="5' Sequence from F-Primer Flap"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aatTAACTCG AGGGATCCAA GCTGGCCGCA
     1141 CGTCTCCCTA TCAGTGATAG AGAAGTCGAC ACGTCTCGAG CTCCCTATCA GTGATAGAGA
     1201 AGGTACGTCT AGAACGTCTC CCTATCAGTG ATAGAGAAGT CGACACGTCT CGAGCTCCCT
     1261 ATCAGTGATA GAGAAGGTAC GTCTAGAACG TCTCCCTATC AGTGATAGAG AAGTCGACAC
     1321 GTCTCGAGCT CCCTATCAGT GATAGAGAAG GTACGTCTAG AACGTCTCCC TATCAGTGAT
     1381 AGAGAAGTCG ACACGTCTCG AGCTCCCTAT CAGTGATAGA GAAGGTACCC CCTATATAAG
     1441 CAGAGCTCGT TTAGTGAACC GTCAGATCGC CTGGAGACGC CATCCACGCT GTTTTGACCT
     1501 CCATAGAAGA CACCGGGACC GATCCAGCCT CCGCGGTGGC GGCCATCACA AGTTTGacta
     1561 gtaccggtta CTCGAGGCTA GCACCATGGC AGAAATCGGT ACTGGCTTTC CATTCGACCC
     1621 CCATTATGTG GAAGTCCTGG GCGAGCGCAT GCACTACGTC GATGTTGGTC CGCGCGATGG
     1681 CACCCCTGTG CTGTTCCTGC ACGGTAACCC GACCTCCTCC TACGTGTGGC GCAACATCAT
     1741 CCCGCATGTT GCACCGACCC ATCGCTGCAT TGCTCCAGAC CTGATCGGTA TGGGCAAATC
     1801 CGACAAACCA GACCTGGGTT ATTTCTTCGA CGACCACGTC CGCTTCATGG ATGCCTTCAT
     1861 CGAAGCCCTG GGTCTGGAAG AGGTCGTCCT GGTCATTCAC GACTGGGGCT CCGCTCTGGG
     1921 TTTCCACTGG GCCAAGCGCA ATCCAGAGCG CGTCAAAGGT ATTGCATTTA TGGAGTTCAT
     1981 CCGCCCTATC CCGACCTGGG ACGAATGGCC AGAATTTGCC CGCGAGACCT TCCAGGCCTT
     2041 CCGCACCACC GACGTCGGCC GCAAGCTGAT CATCGATCAG AACGTTTTTA TCGAGGGTAC
     2101 GCTGCCGATG GGTGTCGTCC GCCCGCTGAC TGAAGTCGAG ATGGACCATT ACCGCGAGCC
     2161 GTTCCTGAAT CCTGTTGACC GCGAGCCACT GTGGCGCTTC CCAAACGAGC TGCCAATCGC
     2221 CGGTGAGCCA GCGAACATCG TCGCGCTGGT CGAAGAATAC ATGGACTGGC TGCACCAGTC
     2281 CCCTGTCCCG AAGCTGCTGT TCTGGGGCAC CCCAGGCGTT CTGATCCCAC CGGCCGAAGC
     2341 CGCTCGCCTG GCCAAAAGCC TGCCTAACTG CAAGGCTGTG GACATCGGCC CGGGTCTGAA
     2401 TCTGCTGCAA GAAGACAACC CGGACCTGAT CGGCAGCGAG ATCGCGCGCT GGCTGTCGAC
     2461 GCTCGAGATT TCCGGCGAGC CAACCACTGA GGATCTGTAC TTTCAGAGCG ATAACGCGAT
     2521 CGCCGGCGCG CCAACAAGTT TGTACAAAAA AGCTGAACGA GAAACGTAAA ATGATATAAA
     2581 TATCAATATA TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA
     2641 ACATATCCAG TCACTATGGC GGCCGCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC
     2701 GGCTCGTATA ATGTGTGGAT TTTGAGTTAG GATCCGTCGA GATTTTCAGG AGCTAAGGAA
     2761 GCTAAAATGG AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT
     2821 AAAGAACATT TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG
     2881 CTGGATATTA CGGCCTTTTT AAAGACCGTA AAGAAAAATA AGCACAAGTT TTATCCGGCC
     2941 TTTATTCACA TTCTTGCCCG CCTGATGAAT GCTCATCCGG AATTCCGTAT GGCAATGAAA
     3001 GACGGTGAGC TGGTGATATG GGATAGTGTT CACCCTTGTT ACACCGTTTT CCATGAGCAA
     3061 ACTGAAACGT TTTCATCGCT CTGGAGTGAA TACCACGACG ATTTCCGGCA GTTTCTACAC
     3121 ATATATTCGC AAGATGTGGC GTGTTACGGT GAAAACCTGG CCTATTTCCC TAAAGGGTTT
     3181 ATTGAGAATA TGTTTTTCGT CTCAGCCAAT CCCTGGGTGA GTTTCACCAG TTTTGATTTA
     3241 AACGTGGCCA ATATGGACAA CTTCTTCGCC CCCGTTTTCA CCATGGGCAA ATATTATACG
     3301 CAAGGCGACA AGGTGCTGAT GCCGCTGGCG ATTCAGGTTC ATCATGCCGT TTGTGATGGC
     3361 TTCCATGTCG GCAGAATGCT TAATGAATTA CAACAGTACT GCGATGAGTG GCAGGGCGGG
     3421 GCGTAAAGAT CTGGATCCcg acaatcaacg cgtggatccg gcttactaaa agccagataa
     3481 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     3541 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     3601 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     3661 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     3721 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
     3781 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     3841 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     3901 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     3961 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     4021 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     4081 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     4141 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     4201 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtg
     4261 atctagctaa aacgcggcct cgaatcgaat tccgcccccc cccctctccc tccccccccc
     4321 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     4381 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     4441 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     4501 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     4561 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     4621 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     4681 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     4741 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     4801 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     4861 aaacacgatg ataatatggc cacaaccatg gtgagcaagg gcgaggagct gttcaccggg
     4921 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     4981 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     5041 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     5101 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     5161 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     5221 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     5281 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     5341 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     5401 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     5461 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     5521 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     5581 ctcggcatgg acgagctgta caagtaaagc ggccgcgatc tttttccctc tgccaaaaat
     5641 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     5701 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     5761 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgccata
     5821 tgctggctgc catgaacaaa ggtggctata aagaggtcat cagtatatga aacagccccc
     5881 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     5941 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     6001 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatgaag
     6061 atccctcgac ctgcaggaat tcgattactt aagcctagga tccagatctg tacattcgaa
     6121 tcgatgaatt cgttaaccat ggaagcttct gcagcggccg cactagcggt ggcggcctcg
     6181 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     6241 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     6301 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     6361 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     6421 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     6481 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     6541 agtcatcgct attaccatga tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     6601 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     6661 actagtggag ccgagagtaa ttcatacaaa aggagggatc gccttcgcaa ggggagagcc
     6721 cagggaccgt ccctaaattc tcacagaccc aaatccctgt agccgcccca cgacagcgcg
     6781 aggagcatgc gcccagggct gagcgcgggt agatcagagc acacaagctc acagtccccg
     6841 gcggtggggg gaggggcgcg ctgagcgggg gccagggagc tggcgcgggg caaactggga
     6901 aagtggtgtc gtgtgctggc tccgccctct tcccgagggt gggggagaac ggtatataag
     6961 tgcggtagtc gccttggacg ttctttttcg caacgggttt gccgtcagaa cgcaggtgag
     7021 tggcgggtgt ggcttccgcg ggccccggag ctggagccct gctctgagcg ggccgggctg
     7081 atatgcgagt gtcgtccgca gggtttagct gtgagcattc ccacttcgag tggcgggcgg
     7141 tgcgggggtg agagtgcgag gcctagcggc aaccccgtag cctcgcctcg tgtccggctt
     7201 gaggcctagc gtggtgtccg ccgccgcgtg ccactccggc cgcactatgc gttttttgtc
     7261 cttgctgccc tcgattgcct tccagcagca tgggctaaca aagggagggt gtggggctca
     7321 ctcttaagga gcccatgaag cttacgttgg ataggaatgg aagggcagga ggggcgactg
     7381 gggcccgccc gccttcggag cacatgtccg acgccacctg gatggggcga ggcctgtggc
     7441 tttccgaagc aatcgggcgt gagtttagcc tacctgggcc atgtggccct agcactgggc
     7501 acggtctggc ctggcggtgc cgcgttccct tgcctcccaa caagggtgag gccgtcccgc
     7561 ccggcaccag ttgcttgcgc ggaaagatgg ccgctcccgg ggccctgttg caaggagctc
     7621 aaaatggagg acgcggcagc ccggtggagc gggcgggtga gtcacccaca caaaggaaga
     7681 gggccttgcc cctcgccggc cgctgcttcc tgtgaccccg tggtctatcg gccgcatagt
     7741 cacctcgggc ttctcttgag caccgctcgt cgcggcgggg ggaggggatc taatggcgtt
     7801 ggagtttgtt cacatttggt gggtggagac tagtcaggcc agcctggcgc tggaagtcat
     7861 tcttggaatt tgcccctttg agtttggagc gaggctaatt ctcaagcctc ttagcggttc
     7921 aaaggtattt tctaaacccg tttccaggtg ttgtgaaagc caccgctaat tcaaagcaat
     7981 ccggagtaaa ttcaccatgt ctagactgga caagagcaaa gtcataaacg gcgctctgga
     8041 attactcaat ggagtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg
     8101 agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct
     8161 gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg
     8221 gcaagacttt ctgcggaaca acgccaagtc attccgctgt gctctcctct cacatcgcga
     8281 cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa
     8341 tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc
     8401 cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa
     8461 agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga
     8521 gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg
     8581 tggcctggag aaacagctaa agtgcgaaag cggcgggccg gccgacgccc ttgacgattt
     8641 tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata tgctgcctgc
     8701 tgacgctctt gacgattttg accttgacat gctccccggg taactaagta aggatcccgt
     8761 acgcctagga gcaggtttcc ccaatgacac aaaacgtgca acttgaaact ccgcctggtc
     8821 tttccaggtc tagaggggta acactttgta ctgcgtttgg ctccacgctc gatccactgg
     8881 cgagtgttag taacagcact gttgcttcgt agcggagcat gacggccgtg ggaactcctc
     8941 cttggtaaca aggacccacg gggccaaaag ccacgcccac acgggcccgt catgtgtgca
     9001 accccagcac ggcgacttta ctgcgaaacc cactttaaag tgacattgaa actggtaccc
     9061 acacactggt gacaggctaa ggatgccctt caggtacccc gaggtaacac gcgacactcg
     9121 ggatctgaga aggggactgg ggcttctata aaagcgctcg gtttaaaaag cttctatgcc
     9181 tgaataggtg accggaggtc ggcacctttc ctttgcaatt actgacccta tgaatacact
     9241 gactgtttga caattaatca tcggcatagt atatcggcat agtataatac gactcactat
     9301 aggagggcca ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     9361 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     9421 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     9481 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     9541 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     9601 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     9661 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     9721 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     9781 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     9841 agcatgcccg acggcgagga tctcgtcgtg acacatggcg atgcctgctt gccgaatatc
     9901 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     9961 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
    10021 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
    10081 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
    10141 cgaattcgct aggattatcc ctaatacctg ccaccccact cttaatcagt ggtggaagaa
    10201 cggtctcaga actgtttgtt tcaattggcc atttaagttt agtagtaaaa gactggttaa
    10261 tgataacaat gcatcgtaaa accttcagaa ggaaaggaga atgttttgtg gaccactttg
    10321 gttttctttt ttgcgtgtgg cagttttaag ttattagttt ttaaaatcag tactttttaa
    10381 tggaaacaac ttgaccaaaa atttgtcaca gaattttgag acccattaaa aaagttaaat
    10441 gagaaacctg tgtgttcctt tggtcaacac cgagacattt aggtgaaaga catctaattc
    10501 tggttttacg aatctggaaa cttcttgaaa atgtaattct tgagttaaca cttctgggtg
    10561 gagaataggg ttgttttccc cccacataat tggaagggga aggaatatca tttaaagcta
    10621 tgggagggtt gctttgatta caacactgga gagaaatgca gcatgttgct gattgcctgt
    10681 cactaaaaca ggccaaaaac tgagtccttg ggttgcatag aaagctgcca aacgctagcc
    10741 aacaagctcg tcatcgcttt gcagaagagc agagaggata tgctcatcgt ctaaagaact
    10801 acccatttta ttatatatta gtcacgatat ctataacaag aaaatatata tataataagt
    10861 tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa atcttaaaag
    10921 tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt tcaaaatcag
    10981 tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga ctgagatgtc
    11041 ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc
    11101 atgcgtcaat tttacgcaga ctatctttct agggttaaaa aagatttgcg ctttactcga
    11161 cctaaacttt aaacacgtca tagaatcttc gtttgacaaa aaccacattg tggccaagct
    11221 gtgtgacgcg acgcgcgcta aagaatggca aaccaagtcg cgcgaggtac ccagcttttg
    11281 ttccctttag tgagggttaa ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt
    11341 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    11401 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    11461 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    11521 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    11581 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    11641 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    11701 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    11761 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    11821 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    11881 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    11941 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    12001 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    12061 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    12121 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
    12181 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    12241 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    12301 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    12361 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    12421 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    12481 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    12541 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    12601 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    12661 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    12721 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    12781 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    12841 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    12901 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    12961 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    13021 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    13081 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    13141 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    13201 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    13261 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    13321 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    13381 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    13441 ggttccgcgc acatttcccc gaaaagtgcc acctg
//