LOCUS       PB-TAG-6HIS-mEGFP-NGFR 13490 bp ds-DNA     circular     11-MAR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1991..2010
                     /label="Primer 7F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(12593..12614)
                     /label="Primer RR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             10471..10506
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    12350..12371
                     /label="Primer FFF"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             10432..10467
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    10377..10398
                     /label="seq"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             9808..10425
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    5107..5127
                     /label="seq"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(804..827)
                     /label="Primer 4R"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(9804..9825)
                     /label="seq"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             3394..3411
                     /label="6xHis"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    6525..6546
                     /label="seq"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             3448..4164
                     /label="EGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    3200..3218
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     CDS             11124..11918
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(1943..1965)
                     /label="Primer 6R"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1348..1369
                     /label="Primer 5F"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    13227..13248
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     CDS             10426..10431
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    8326..8347
                     /label="seq"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2312..2331)
                     /label="Primer 8R"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    3126..3144
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     repeat_region   2507..2569
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(13008..13029)
                     /label="Primer RRR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    6531..6533
                     /label="IRES2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     repeat_region   12911..12945
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein_bind    13158..13174
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    3052..3070
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     CDS             10507..10509
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    10126..10146
                     /label="seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4739..4760
                     /label="seq"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    11093..11117
                     /label="FFFF"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4039..4058
                     /label="SeqF for LR Frame"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(46..634)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5956..6530
                     /label="IRES2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    5501..5525
                     /label="seq"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             4422..5081
                     /label="Chlor. Resistance"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             10510..10545
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    7655..7680
                     /label="seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4189..4313
                     /label="attR1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     polyA_site      7495..7550
                     /label="β-globin poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        complement(13095..13113)
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             10546..10554
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    3163..3181
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    2941..2959
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    complement(7457..7476)
                     /label="REV"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     protein_bind    3089..3107
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        2422..2440
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    9751..9771
                     /label="seq"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     polyA_signal    11965..12537
                     /label="EF-1α poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(638..660)
                     /label="Primer 2R"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(5910..5930)
                     /label="rev"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     enhancer        7991..8370
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      complement(1799..2254)
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(13469..13490)
                     /label="Primer R"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    9062..9081
                     /label="seq"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(7399..7418)
                     /label="New Rev_NGFR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4394..4416
                     /label="seq"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             complement(1597..1665)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3372..3381
                     /label="Ward Kozak"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    12954..12973
                     /label="Primer F"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    478..502
                     /label="Primer 2F"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(13207..13212)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(10628..10647)
                     /label="seq"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             10468..10470
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        complement(1666..1770)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        11058..11104
                     /label="EM7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    6543..7418
                     /label="NGFR ORF"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     2399..2415
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     protein_bind    complement(5790..5914)
                     /label="attR2"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(13182..13188)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    11932..11951
                     /label="Primer FF"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(805..1596)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    8618..8638
                     /label="seq"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(9480..9499)
                     /label="rev"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    7326..7346
                     /label="seq"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4187..4210
                     /label="seq"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3418..3438
                     /label="TEV Protease CDS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    42..61
                     /label="primer 1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        complement(13189..13206)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(8423..8442)
                     /label="seq"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        11096..11114
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    8763..8783
                     /label="seq"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(2903..2924)
                     /label="Primer 10R"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             5444..5749
                     /label="ccdB"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    4066..4068
                     /label="monomeric mutation"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(13134..13150)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     misc_feature    10581..11025
                     /label="FMDV IRES"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(11256..11275)
                     /label="RRRR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3876..3897
                     /label="SeqF2_LR Frame"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     protein_bind    2978..2996
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    complement(5004..5024)
                     /label="Rev"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2357..2377
                     /label="Primer 9F"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3237..3256
                     /label="seq"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    6534..6542
                     /label="IRES2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(7183..7204)
                     /label="seq"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    3015..3033
                     /label="tet operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     primer          7419..7438
                     /label="PB-TAG-Halo-ERN Destination Vector Final Design FWD"
                     /note="sequence: GCAGCCAAGCTTATCGATAAagcggccgcgatctttttcc"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(6519..6542)
                     /label="PB-TAG-Halo-ERN Destination Vector Final Design REV"
                     /note="sequence: CCGTCCATGGTTGTGGCCATggttgtggccatattatcatcgtg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(7397..7418)
                     /label="Plasmid # 27489 - NGFR from AddGene Original REV"
                     /note="sequence: ggaaaaagatcgcggccgctTTATCGATAAGCTTGGCTGCAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          6543..6562
                     /label="Plasmid # 27489 - NGFR from AddGene Original FWD"
                     /note="sequence: atgataatatggccacaaccATGGCCACAACCATGGACGG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
       61 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      121 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      181 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      241 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      301 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      361 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      421 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      481 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      541 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      601 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
      661 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
      721 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
      781 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
      841 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
      901 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
      961 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     1021 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     1081 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     1141 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     1201 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     1261 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     1321 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     1381 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     1441 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     1501 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     1561 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     1621 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     1681 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     1741 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggaa
     1801 attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     1861 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     1921 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     1981 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     2041 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     2101 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     2161 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     2221 cccgccgcgc ttaatgcgcc gctacagggc gcgtcgcgcc attcgccatt caggctgcgc
     2281 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     2341 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     2401 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattgga gctcggtatt
     2461 cacgacagca ggctgaataa taaaaaaatt agaaactatt atttaaccct agaaagataa
     2521 tcatattgtg acgtacgtta aagataatca tgcgtaaaat tgacgcatgt gttttatcgg
     2581 tctgtatatc gaggtttatt tattaatttg aatagatatt aagttttatt atatttacac
     2641 ttacatacta ataataaatt caacaaacaa tttatttatg tttatttatt tattaaaaaa
     2701 aaacaaaaac tcaaaatttc ttctataaag taacaaaact tttaaacatt ctctctttta
     2761 caaaaataaa cttattttgt actttaaaaa cagtcatgtt gtattataaa ataagtaatt
     2821 agcttaacct atacataata gaaacaaatt atacttatta gtcagtcaga aacaactttg
     2881 gcacatatca atattatgct ctcgttaatT AACTCGAGGG ATCCAAGCTG GCCGCACGTC
     2941 TCCCTATCAG TGATAGAGAA GTCGACACGT CTCGAGCTCC CTATCAGTGA TAGAGAAGGT
     3001 ACGTCTAGAA CGTCTCCCTA TCAGTGATAG AGAAGTCGAC ACGTCTCGAG CTCCCTATCA
     3061 GTGATAGAGA AGGTACGTCT AGAACGTCTC CCTATCAGTG ATAGAGAAGT CGACACGTCT
     3121 CGAGCTCCCT ATCAGTGATA GAGAAGGTAC GTCTAGAACG TCTCCCTATC AGTGATAGAG
     3181 AAGTCGACAC GTCTCGAGCT CCCTATCAGT GATAGAGAAG GTACCCCCTA TATAAGCAGA
     3241 GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT
     3301 AGAAGACACC GGGACCGATC CAGCCTCCGC GGTGGCGGCC ATCACAAGTT TGactagtaC
     3361 CGGTTACTCG AGGCTAGCAC Catgggcagc agccatcatc atcatcatca cagcagcgag
     3421 gatctgtact ttcagagcgg cagcagcATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG
     3481 GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC
     3541 GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC
     3601 GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC
     3661 TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA
     3721 GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC
     3781 GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC
     3841 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC
     3901 TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC
     3961 ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC
     4021 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCaagCT GAGCAAAGAC
     4081 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT
     4141 CTCGGCATGG ACGAGCTGTA CAAGGATAAC GCGATCGCCG GCGCGCCAAC AAGTTTGTAC
     4201 AAAAAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA ATATATTAAA TTAGATTTTG
     4261 CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA TCCAGTCACT ATGGCGGCCG
     4321 CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATAATGTG TGGATTTTGA
     4381 GTTAGGATCC GTCGAGATTT TCAGGAGCTA AGGAAGCTAA AATGGAGAAA AAAATCACTG
     4441 GATATACCAC CGTTGATATA TCCCAATGGC ATCGTAAAGA ACATTTTGAG GCATTTCAGT
     4501 CAGTTGCTCA ATGTACCTAT AACCAGACCG TTCAGCTGGA TATTACGGCC TTTTTAAAGA
     4561 CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGCCTTTAT TCACATTCTT GCCCGCCTGA
     4621 TGAATGCTCA TCCGGAATTC CGTATGGCAA TGAAAGACGG TGAGCTGGTG ATATGGGATA
     4681 GTGTTCACCC TTGTTACACC GTTTTCCATG AGCAAACTGA AACGTTTTCA TCGCTCTGGA
     4741 GTGAATACCA CGACGATTTC CGGCAGTTTC TACACATATA TTCGCAAGAT GTGGCGTGTT
     4801 ACGGTGAAAA CCTGGCCTAT TTCCCTAAAG GGTTTATTGA GAATATGTTT TTCGTCTCAG
     4861 CCAATCCCTG GGTGAGTTTC ACCAGTTTTG ATTTAAACGT GGCCAATATG GACAACTTCT
     4921 TCGCCCCCGT TTTCACCATG GGCAAATATT ATACGCAAGG CGACAAGGTG CTGATGCCGC
     4981 TGGCGATTCA GGTTCATCAT GCCGTTTGTG ATGGCTTCCA TGTCGGCAGA ATGCTTAATG
     5041 AATTACAACA GTACTGCGAT GAGTGGCAGG GCGGGGCGTA AAGATCTGGA TCCcgacaat
     5101 caacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
     5161 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
     5221 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
     5281 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
     5341 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
     5401 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
     5461 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
     5521 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
     5581 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
     5641 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
     5701 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
     5761 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt
     5821 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     5881 acgtttctcg ttcagctttc ttgtacaaag tggtgatcta gctaaaacgc ggcctcgaat
     5941 cgaattccgc ccccccccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     6001 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     6061 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     6121 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     6181 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     6241 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     6301 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     6361 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     6421 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     6481 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     6541 ccATGGCCAC AACCATGGAC GGGCCGCGCC TGCTGCTGTT GCTGCTTCTG GGGGTGTCCC
     6601 TTGGAGGTGC CAAGGAGGCA TGCCCCACAG GCCTGTACAC ACACAGCGGT GAGTGCTGCA
     6661 AAGCCTGCAA CCTGGGCGAG GGTGTGGCCC AGCCTTGTGG AGCCAACCAG ACCGTGTGTG
     6721 AGCCCTGCCT GGACAGCGTG ACGTTCTCCG ACGTGGTGAG CGCGACCGAG CCGTGCAAGC
     6781 CGTGCACCGA GTGCGTGGGG CTCCAGAGCA TGTCGGCGCC GTGCGTGGAG GCCGACGACG
     6841 CCGTGTGCCG CTGCGCCTAC GGCTACTACC AGGATGAGAC GACTGGGCGC TGCGAGGCGT
     6901 GCCGCGTGTG CGAGGCGGGC TCGGGCCTCG TGTTCTCCTG CCAGGACAAG CAGAACACCG
     6961 TGTGCGAGGA GTGCCCCGAC GGCACGTATT CCGACGAGGC CAACCACGTG GACCCGTGCC
     7021 TGCCCTGCAC CGTGTGCGAG GACACCGAGC GCCAGCTCCG CGAGTGCACA CGCTGGGCCG
     7081 ACGCCGAGTG CGAGGAGATC CCTGGCCGTT GGATTACACG GTCCACACCC CCAGAGGGCT
     7141 CGGACAGCAC AGCCCCCAGC ACCCAGGAGC CTGAGGCACC TCCAGAACAA GACCTCATAG
     7201 CCAGCACGGT GGCAGGTGTG GTGACCACAG TGATGGGCAG CTCCCAGCCC GTGGTGACCC
     7261 GAGGCACCAC CGACAACCTC ATCCCTGTCT ATTGCTCCAT CCTGGCTGCT GTGGTTGTGG
     7321 GCCTTGTGGC CTACATAGCC TTCAAGAGGT GGAACAGTCA TCGATATCCT CGAGGTCACC
     7381 GCGGTCTAGA GTCGACCTGC AGCCAAGCTT ATCGATAAag cggccgcgat ctttttccct
     7441 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     7501 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     7561 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     7621 catatgccat atgctggctg ccatgaacaa aggtggctat aaagaggtca tcagtatatg
     7681 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     7741 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     7801 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     7861 ctcttatgaa gatccctcga cctgcaggaa ttcgattact taagcctagg atccagatct
     7921 gtacattcga atcgatgaat tcgttaacca tggaagcttc tgcagcggcc gcactagcgg
     7981 tggcggcctc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     8041 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     8101 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     8161 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     8221 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     8281 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     8341 atctacgtat tagtcatcgc tattaccatg atgatgcggt tttggcagta catcaatggg
     8401 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     8461 agtttgtttt gactagtgga gccgagagta attcatacaa aaggagggat cgccttcgca
     8521 aggggagagc ccagggaccg tccctaaatt ctcacagacc caaatccctg tagccgcccc
     8581 acgacagcgc gaggagcatg cgcccagggc tgagcgcggg tagatcagag cacacaagct
     8641 cacagtcccc ggcggtgggg ggaggggcgc gctgagcggg ggccagggag ctggcgcggg
     8701 gcaaactggg aaagtggtgt cgtgtgctgg ctccgccctc ttcccgaggg tgggggagaa
     8761 cggtatataa gtgcggtagt cgccttggac gttctttttc gcaacgggtt tgccgtcaga
     8821 acgcaggtga gtggcgggtg tggcttccgc gggccccgga gctggagccc tgctctgagc
     8881 gggccgggct gatatgcgag tgtcgtccgc agggtttagc tgtgagcatt cccacttcga
     8941 gtggcgggcg gtgcgggggt gagagtgcga ggcctagcgg caaccccgta gcctcgcctc
     9001 gtgtccggct tgaggcctag cgtggtgtcc gccgccgcgt gccactccgg ccgcactatg
     9061 cgttttttgt ccttgctgcc ctcgattgcc ttccagcagc atgggctaac aaagggaggg
     9121 tgtggggctc actcttaagg agcccatgaa gcttacgttg gataggaatg gaagggcagg
     9181 aggggcgact ggggcccgcc cgccttcgga gcacatgtcc gacgccacct ggatggggcg
     9241 aggcctgtgg ctttccgaag caatcgggcg tgagtttagc ctacctgggc catgtggccc
     9301 tagcactggg cacggtctgg cctggcggtg ccgcgttccc ttgcctccca acaagggtga
     9361 ggccgtcccg cccggcacca gttgcttgcg cggaaagatg gccgctcccg gggccctgtt
     9421 gcaaggagct caaaatggag gacgcggcag cccggtggag cgggcgggtg agtcacccac
     9481 acaaaggaag agggccttgc ccctcgccgg ccgctgcttc ctgtgacccc gtggtctatc
     9541 ggccgcatag tcacctcggg cttctcttga gcaccgctcg tcgcggcggg gggaggggat
     9601 ctaatggcgt tggagtttgt tcacatttgg tgggtggaga ctagtcaggc cagcctggcg
     9661 ctggaagtca ttcttggaat ttgccccttt gagtttggag cgaggctaat tctcaagcct
     9721 cttagcggtt caaaggtatt ttctaaaccc gtttccaggt gttgtgaaag ccaccgctaa
     9781 ttcaaagcaa tccggagtaa attcaccatg tctagactgg acaagagcaa agtcataaac
     9841 ggcgctctgg aattactcaa tggagtcggt atcgaaggcc tgacgacaag gaaactcgct
     9901 caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg
     9961 ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa
    10021 ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cattccgctg tgctctcctc
    10081 tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa
    10141 accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg
    10201 tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat
    10261 caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga
    10321 caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa
    10381 ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc ggccgacgcc
    10441 cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat
    10501 atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaactaagt
    10561 aaggatcccg tacgcctagg agcaggtttc cccaatgaca caaaacgtgc aacttgaaac
    10621 tccgcctggt ctttccaggt ctagaggggt aacactttgt actgcgtttg gctccacgct
    10681 cgatccactg gcgagtgtta gtaacagcac tgttgcttcg tagcggagca tgacggccgt
    10741 gggaactcct ccttggtaac aaggacccac ggggccaaaa gccacgccca cacgggcccg
    10801 tcatgtgtgc aaccccagca cggcgacttt actgcgaaac ccactttaaa gtgacattga
    10861 aactggtacc cacacactgg tgacaggcta aggatgccct tcaggtaccc cgaggtaaca
    10921 cgcgacactc gggatctgag aaggggactg gggcttctat aaaagcgctc ggtttaaaaa
    10981 gcttctatgc ctgaataggt gaccggaggt cggcaccttt cctttgcaat tactgaccct
    11041 atgaatacac tgactgtttg acaattaatc atcggcatag tatatcggca tagtataata
    11101 cgactcacta taggagggcc accatgattg aacaagatgg attgcacgca ggttctccgg
    11161 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
    11221 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
    11281 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
    11341 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
    11401 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
    11461 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
    11521 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
    11581 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
    11641 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacacatggc gatgcctgct
    11701 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
    11761 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
    11821 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
    11881 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
    11941 gaccgaccaa gcgaattcgc taggattatc cctaatacct gccaccccac tcttaatcag
    12001 tggtggaaga acggtctcag aactgtttgt ttcaattggc catttaagtt tagtagtaaa
    12061 agactggtta atgataacaa tgcatcgtaa aaccttcaga aggaaaggag aatgttttgt
    12121 ggaccacttt ggttttcttt tttgcgtgtg gcagttttaa gttattagtt tttaaaatca
    12181 gtacttttta atggaaacaa cttgaccaaa aatttgtcac agaattttga gacccattaa
    12241 aaaagttaaa tgagaaacct gtgtgttcct ttggtcaaca ccgagacatt taggtgaaag
    12301 acatctaatt ctggttttac gaatctggaa acttcttgaa aatgtaattc ttgagttaac
    12361 acttctgggt ggagaatagg gttgttttcc ccccacataa ttggaagggg aaggaatatc
    12421 atttaaagct atgggagggt tgctttgatt acaacactgg agagaaatgc agcatgttgc
    12481 tgattgcctg tcactaaaac aggccaaaaa ctgagtcctt gggttgcata gaaagctgcc
    12541 aaacgctagc caacaagctc gtcatcgctt tgcagaagag cagagaggat atgctcatcg
    12601 tctaaagaac tacccatttt attatatatt agtcacgata tctataacaa gaaaatatat
    12661 atataataag ttatcacgta agtagaacat gaaataacaa tataattatc gtatgagtta
    12721 aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt gactcacgcg gtcgttatag
    12781 ttcaaaatca gtgacactta ccgcattgac aagcacgcct cacgggagct ccaagcggcg
    12841 actgagatgt cctaaatgca cagcgacgga ttcgcgctat ttagaaagag agagcaatat
    12901 ttcaagaatg catgcgtcaa ttttacgcag actatctttc tagggttaaa aaagatttgc
    12961 gctttactcg acctaaactt taaacacgtc atagaatctt cgtttgacaa aaaccacatt
    13021 gtggccaagc tgtgtgacgc gacgcgcgct aaagaatggc aaaccaagtc gcgcgaggta
    13081 cccagctttt gttcccttta gtgagggtta attccgagct tggcgtaatc atggtcatag
    13141 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    13201 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    13261 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    13321 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    13381 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    13441 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
//