LOCUS       Exported                8077 bp ds-DNA     circular SYN 06-APR-2016
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    MS03_PHAGE_DN_CMV_hSHH
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   Pazour Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 10, 2020 from SnapGene 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8077
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2061..2178
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2835..3428
                     /codon_start=1
                     /label=hSHH
                     /note="color: #a6acb3"
                     /translation="MLLLARCLLLVLVSSLLVCSGLACGPGRGFGKRRHPKKLTPLAYK
                     QFIPNVAEKTLGASGRYEGKISRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKD
                     KLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLAR
                     LAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG"
     misc_feature    3460..4046
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (ECMV)"
                     /note="This feature has 3 segments:
                        1: 3460 .. 4034 / #a6acb3
                        2: 4035 .. 4037 / #a6acb3 / ATG
                        3: 4038 .. 4046 / #a6acb3"
     primer_bind     4039..4056
                     /label=Primer 1
                     /note="color: black; sequence: CCACAACCATGGCCAAGC"
     CDS             4047..4442
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /note="color: #ccffcc"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(4492..4512)
                     /label=Primer 2
                     /note="color: black; sequence: 
                     TTTTCCTTTTCTCGAGAGGGCCGGGATTCT"
     misc_feature    4519..5107
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(4990..5001)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 4989"
                     /translation="IEGR"
     LTR             5255..5435
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     promoter        5568..5672
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5673..6533
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5673 .. 5741 / #ccffcc / signal sequence
                        2: 5742 .. 6533 / #ccffcc
                     Cleavage site after base 5741"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6704..7292
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     promoter        7739..7769
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 7739 .. 7744 / #ffffff / -35
                        2: 7745 .. 7762 / #ffffff
                        3: 7763 .. 7769 / #ffffff / -10"
     protein_bind    7777..7793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     7801..7817
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctgcagagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttctcaacaa
     2041 taggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2101 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2161 acaaaaattc aaaattttcg ggtttattac tcggacagca gagatccagt ttggactagt
     2221 ggagttccgc gttacataac ttacgcaaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgaccaatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacgcaa aactgcccac ttggcagtac atcaagtgta
     2401 tcatttgcca acaacgcccc ctattgacgt caatgacgca aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccattcaaga caccggcggc cttaattaat
     2821 gatcaggatc caccatgctg ctgctggcga gatgtctgct gctagtcctc gtctcctcgc
     2881 tgctggtatg ctcgggactg gcgtgcggac cgggcagggg gttcgggaag aggaggcacc
     2941 ccaaaaagct gaccccttta gcctacaagc agtttatccc caatgtggcc gagaagaccc
     3001 taggcgccag cggaaggtat gaagggaaga tctccagaaa ctccgagcga tttaaggaac
     3061 tcacccccaa ttacaacccc gacatcatat ttaaggatga agaaaacacc ggagcggaca
     3121 ggctgatgac tcagaggtgt aaggacaagt tgaacgcttt ggccatctcg gtgatgaacc
     3181 agtggccagg agtgaaactg cgggtgaccg agggctggga cgaagatggc caccactcag
     3241 aggagtctct gcactacgag ggccgcgcag tggacatcac cacgtctgac cgcgaccgca
     3301 gcaagtacgg catgctggcc cgcctggcgg tggaggccgg cttcgactgg gtgtactacg
     3361 agtccaaggc acatatccac tgctcggtga aagcagagaa ctcggtggcg gccaaatcgg
     3421 gaggctaatc tagagcggcc gctaagtaag taagatccgc ccctctccct cccccccccc
     3481 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     3541 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     3601 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     3661 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     3721 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     3781 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     3841 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     3901 ggtaccccat tgtatgggat ctgatctggg gcctcggtac acatgcttta catgtgttta
     3961 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     4021 aacacgatga taatatggcc acaaccatgg ccaagccttt gtctcaagaa gaatccaccc
     4081 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     4141 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     4201 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     4261 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     4321 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     4381 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     4441 gcggtagtgg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg
     4501 gccctctcga gagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4561 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4621 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4681 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4741 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4801 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4861 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     4921 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4981 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5041 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5101 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     5161 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatcacct
     5221 gcaggacagg cgcgccctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5281 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5341 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5401 cagacccttt tagtcagtgt ggaaaatctc tagcacccgg gcgattaagg aaagggctag
     5461 atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     5521 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5581 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5641 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5701 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5761 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5821 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5881 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     5941 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6001 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6061 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6121 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6181 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6241 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6301 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6361 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6421 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6481 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6541 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6601 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6661 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6721 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6781 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6841 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6901 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6961 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7021 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7081 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7141 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7201 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7261 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7321 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7381 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7441 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     7501 ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt tttttattta
     7561 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7621 tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag
     7681 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     7741 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     7801 caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt
     7861 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
     7921 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
     7981 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
     8041 attgtatttg ttaaatatgt actacaaact tagtagt
//
