LOCUS Exported 9770 bp ds-DNA circular SYN 19-JUL-2018 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 9770) AUTHORS Pazour Lab TITLE Direct Submission JOURNAL Exported Thursday, Dec 10, 2020 from SnapGene 5.2.3 https://www.snapgene.com FEATURES Location/Qualifiers source 1..9770 /organism="recombinant plasmid" /mol_type="other DNA" primer_bind complement(1..20) /label=HIV_Rev /note="color: black; sequence: gatctgagggctcgccactc" primer_bind 1..20 /label=HIV_For /note="color: black; sequence: gagtggcgagccctcagatc" misc_feature 280..405 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 902..1135 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" primer_bind 1586..1604 /label=Phage_5'_promoter_seq /note="color: black; sequence: cagatccattcgattagtg" misc_feature 1660..1777 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" /note="color: #ffe4c4" primer_bind 1795..1855 /label=SV40polyA_For /note="color: black; sequence: cagcagagatccagtttggactagtGGCCGCAATAAAATATCTTTATTTTCATTACATC TG" polyA_signal 1826..1874 /note="synthetic polyadenylation signal" /note="color: #a6acb3" misc_feature 1888..1979 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" /note="color: #a6acb3" polyA_signal 1998..2132 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" /note="color: #a6acb3" primer_bind complement(2132..2175) /label=SV40polyA_Rev /note="color: black; sequence: acccctatcctgtgtgtccactagtCGCGAATCTGTTCCCGGGG" primer_bind 2133..2150 /label=Fragment.FOR /note="color: black; sequence: tggaCTAGTCCAGTGTTTAATCCCGGGAACAGATTCGCG" primer_bind 2150..2177 /label=GgCry_For /note="color: black; sequence: cagcagagatccagtttggactagtggacacacaggataggggtgg" promoter 2157..2320 /label=Chicken delta-1-crystallin gene, promoter region. /note="color: #ffffff" primer_bind 2250..2264 /label=Cry_For_seq /note="color: black; sequence: gtgacgagctgccag" primer_bind complement(2296..2326) /label=Smo_GgCryD1_Rev2 /note="color: black; sequence: gccatggtCtgatcattaatgtcgctccgtgctctccggtctcac" primer_bind complement(2303..2320) /label=GgCry_Rev /note="color: black; sequence: GTCTTTGTAGTCCATGGTGGGATCCgtcgctccgtgctctccg" primer_bind 2307..2348 /label=BSD2_FOR /note="color: black; sequence: gagcacggagcgacattaatatggccaagcctttgtctcaag" mRNA 2311..2320 /product="delta-1 crystallin mRNA" /label=delta-1 crystallin mRNA /note="color: #a6acb3; direction: RIGHT" CDS 2327..2722 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /note="color: #ccffcc" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" primer_bind 2380..2403 /label=Sequences /note="color: black; sequence: tacaatcaacagcatccccatctc" primer_bind complement(2703..2732) /label=BSD2_REV /note="color: black; sequence: gggagagggggccctcccacacataaccag" primer_bind complement(2703..2722) /label=WPRE to IRES /note="color: black; sequence: ccagaggttgattagGATCTCTCGAGttagccctcccacacataaccag" primer_bind 2713..2740 /label=Ires2_FOR /note="color: black; sequence: gtgggagggccccctctccctccccccc" misc_feature 2723..3309 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV)" /note="This feature has 3 segments: 1: 2723 .. 3297 / #a6acb3 2: 3298 .. 3300 / #a6acb3 / ATG 3: 3301 .. 3309 / #a6acb3" primer_bind 3118..3137 /label=IRES_seq_For /note="color: black; sequence: ggctctcctcaagcgtattc" primer_bind complement(3285..3331) /label=Ires2_REV /note="color: black; sequence: gcggccagcggccatggtCtatggttgtggccatattatcatcgtgt" CDS 3310..3311 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /note="color: #ccffcc" /translation="" primer_bind 3312..3327 /label=Smo_F2 /note="color: black; sequence: CGGCCttaattaatgatcaGaccatggccgctgg" gene 3315..5695 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /label=Smo /note="smoothened, frizzled class receptor" /note="color: #a6acb3; direction: RIGHT" /db_xref="GeneID:319757" /db_xref="MGI:MGI:108075" exon 3315..3659 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /note="color: #708090" /inference="alignment:same species:1.39.8" CDS 3317..5695 /codon_start=1 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /product="smoothened homolog precursor" /label=Smo /note="bent body; smoothened homolog" /note="color: #993366" /db_xref="CCDS:CCDS19965.1" /db_xref="GeneID:319757" /db_xref="MGI:MGI:108075" /protein_id="NP_795970.3" /translation="MAAGRPVRGPELAPRRLLQLLLLVLLGGPGRGAALSGNVTGPGPH SASGSSRRDVPVTSPPPPLLSHCGRAAHCEPLRYNVCLGSALPYGATTTLLAGDSDSQE EAHGKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVER ERGWPDFLRCTPDHFPEGCPNEVQNIKFNSSGQCEAPLVRTDNPKSWYEDVEGCGIQCQ NPLFTEAEHQDMHSYIAAFGAVTGLCTLFTLATFVADWRNSNRYPAVILFYVNACFFVG SIGWLAQFMDGARREIVCRADGTMRFGEPTSSETLSCVIIFVIVYYALMAGVVWFVVLT YAWHTSFKALGTTYQPLSGKTSYFHLLTWSLPFVLTVAILAVAQVDGDSVSGICFVGYK NYRYRAGFVLAPIGLVLIVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETMLRLGI FGFLAFGFVLITFSCHFYDFFNQAEWERSFRDYVLCQANVTIGLPTKKPIPDCEIKNRP SLLVEKINLFAMFGTGIAMSTWVWTKATLLIWRRTWCRLTGHSDDEPKRIKKSKMIAKA FSKRRELLQNPGQELSFSMHTVSHDGPVAGLAFDLNEPSADVSSAWAQHVTKMVARRGA ILPQDVSVTPVATPVPPEEQANMWLVEAEISPELEKRLGRKKKRRKRKKEVCPLRPAPE LHHSAPVPATSAVPRLPQLPRQKCLVAANAWGTGESCRQGAWTLVSNPFCPEPSPHQDP FLPGASAPRVWAQGRLQGLGSIHSRTNLMEAEILDADSDF" sig_peptide 3317..3412 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /note="color: #cc99b2; direction: RIGHT" /inference="COORDINATES:same species:SignalP" mat_peptide 3413..5695 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /product="Smoothened homolog" /label=propagated from UniProtKB/Swiss-Prot (P56726.2) /note="propagated from UniProtKB/Swiss-Prot (P56726.2)" /note="color: #cc99b2; direction: RIGHT" /experiment="experimental evidence, no additional details recorded" exon 3660..3865 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /note="color: #708090" /inference="alignment:same species:1.39.8" exon 3866..4075 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /note="color: #708090" /inference="alignment:same species:1.39.8" misc_feature 4028..4090 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /label=Transmembrane 1 /note="propagated from UniProtKB/Swiss-Prot (P56726.2); transmembrane region" /note="color: #a6acb3" /experiment="experimental evidence, no additional details recorded" exon 4076..4248 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /note="color: #708090" /inference="alignment:same species:1.39.8" misc_feature 4112..4174 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /label=Transmembrane 2 /note="propagated from UniProtKB/Swiss-Prot (P56726.2); transmembrane region" /note="color: #a6acb3" /experiment="experimental evidence, no additional details recorded" exon 4249..4468 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /note="color: #708090" /inference="alignment:same species:1.39.8" misc_feature 4271..4333 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /label=Transmembrane 3 /note="propagated from UniProtKB/Swiss-Prot (P56726.2); transmembrane region" /note="color: #a6acb3" /experiment="experimental evidence, no additional details recorded" misc_feature 4403..4465 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /label=Transmembrane 4 /note="propagated from UniProtKB/Swiss-Prot (P56726.2); transmembrane region" /note="color: #a6acb3" /experiment="experimental evidence, no additional details recorded" exon 4469..4592 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /note="color: #708090" /inference="alignment:same species:1.39.8" misc_feature 4535..4597 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /label=Transmembrane 5 /note="propagated from UniProtKB/Swiss-Prot (P56726.2); transmembrane region" /note="color: #a6acb3" /experiment="experimental evidence, no additional details recorded" exon 4593..4685 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /note="color: #708090" /inference="alignment:same species:1.39.8" primer_bind complement(4597..4641) /label=MmSmo_Loop3_R_wt /note="color: black; sequence: ctcagaagcccagggtggttgctcttgatggagaacagagtcatg" primer_bind complement(4597..4641) /label=MmSmo_Loop3_R /note="color: black; sequence: ctcagaagcccagggtggttgctcctgatggagaacagagtcatg" primer_bind 4621..4680 /label=MmSmo_Loop3_F_K3 /note="color: black; sequence: caaccaccctgggcttctgagtgagaaggcagccagcaggatcaacgagaccatgctgc g" primer_bind 4621..4680 /label=MmSmo_Loop3_F_K2 /note="color: black; sequence: caaccaccctgggcttctgagtgagagggcagccagcaagatcaacgagaccatgctgc g" primer_bind 4621..4680 /label=MmSmo_Loop3_F_wt /note="color: black; sequence: caaccaccctgggcttctgagtgagaaggcagccagcaagatcaacgagaccatgctgc g" primer_bind 4621..4680 /label=MmSmo_loop3_F /note="color: black; sequence: caaccaccctgggcttctgagtgagagggcagccagcaggatcaacgagaccatgctgc g" misc_feature 4682..4744 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /label=Transmembrane 6 /note="propagated from UniProtKB/Swiss-Prot (P56726.2); transmembrane region" /note="color: #a6acb3" /experiment="experimental evidence, no additional details recorded" exon 4686..4794 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /note="color: #708090" /inference="alignment:same species:1.39.8" exon 4795..4980 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /note="color: #708090" /inference="alignment:same species:1.39.8" misc_feature 4901..4963 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /label=Transmembrane 7 /note="propagated from UniProtKB/Swiss-Prot (P56726.2); transmembrane region" /note="color: #a6acb3" /experiment="experimental evidence, no additional details recorded" misc_feature 4940..5035 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /note="propagated from UniProtKB/Swiss-Prot (P56726.2); Region: Interaction with BBS5 and BBS7" /note="color: #a6acb3" /experiment="experimental evidence, no additional details recorded" exon 4981..5129 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /note="color: #708090" /inference="alignment:same species:1.39.8" exon 5130..5264 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /note="color: #708090" /inference="alignment:same species:1.39.8" exon 5265..5695 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /note="color: #708090" /inference="alignment:same species:1.39.8" STS 5346..5695 /gene="Smo" /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened" /standard_name="Smo" /label=Smo /note="color: #a6acb3" /db_xref="UniSTS:546036" primer_bind complement(5678..5716) /label=Smo_R2 /note="color: black; sequence: GTCTTTGTAGTCCATGGTGGCgaagtccgagtctgcatc" CDS 5705..5770 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /note="color: #cc99b2 Cleavage site after base 5770" /translation="DYKDHDGDYKDHDIDYKDDDDK" primer_bind complement(5743..5770) /label=Flag_Reverse /note="color: black; sequence: cttgtcatcgtcatccttgtaatcgatg" primer_bind complement(5746..5795) /label=Flag-Rev /note="color: black; sequence: cGGATCttacttactAaGCGGCCGCCTTGTCATCGTCATCCTTGTAATCG" primer_bind 5746..5795 /label=IRES to WPRE /note="color: black; sequence: CGATTACAAGGATGACGATGACAAGGCGGCCGCtTagtaagtaaGATCCg" primer_bind 5776..5823 /label=WPRE-For /note="color: black; sequence: CGCtTagtaagtaaGATCCgaaCTCGAGAGATCctaatcaacctctgg" primer_bind 5796..5823 /label=Fragment 3.FOR /note="color: black; sequence: ctggttatgtgtgggagggctaaCTCGAGAGATCctaatcaacctctgg" primer_bind complement(5803..5823) /label=Primer 1 /note="color: black; sequence: ccagaggttgattagGATCTC" misc_feature 5811..6399 /label=WPRE /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(6282..6293) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /note="color: #cc99b2 Cleavage site after base 6281" /translation="IEGR" LTR 6547..6727 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" promoter 6860..6964 /gene="bla" /label=AmpR promoter /note="color: #ffffff; direction: RIGHT" CDS 6965..7825 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 6965 .. 7033 / #ccffcc / signal sequence 2: 7034 .. 7825 / #ccffcc Cleavage site after base 7033" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7317..7345) /label=Greg_Amp_Ra /note="color: black; sequence: catggttatGGCAGCACTGCATAATTCTc" primer_bind 7317..7345 /label=Greg_Amp_Fa /note="color: black; sequence: gAGAATTATGCAGTGCTGCCataaccatg" primer_bind complement(7317..7345) /label=Greg_Amp_R /note="color: black; sequence: CATGGTTATGGCAGCACTGCATAATTCTC" primer_bind 7317..7345 /label=Greg_Amp_F /note="color: black; sequence: GAGAATTATGCAGTGCTGCCATAACCATG" primer_bind complement(7318..7336) /label=Fragment 3.REV /note="color: black; sequence: GGCAGCACTGCATAATTCT" primer_bind 7318..7336 /label=Fragment 2.REV /note="color: black; sequence: AGAATTATGCAGTGCTGCC" rep_origin 7996..8584 /direction=RIGHT /label=ori /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" promoter 9031..9061 /label=lac promoter /note="promoter for the E. coli lac operon" /note="This forward directional feature has 3 segments: 1: 9031 .. 9036 / #ffffff / -35 2: 9037 .. 9054 / #ffffff 3: 9055 .. 9061 / #ffffff / -10" protein_bind 9069..9085 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /note="color: #31849b" primer_bind 9076..9095 /label=M13-48REV /note="color: black; sequence: CGGATAACAATTTCACACAG" primer_bind 9093..9109 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" /note="color: #a020f0; direction: RIGHT" primer_bind 9093..9109 /label=M13R /note="color: black; sequence: CAGGAAACAGCTATGAC" LTR join(9370..9770,1..233) /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" ORIGIN 1 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct 61 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 121 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 181 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 241 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg 301 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tgcagagtac gccaaaaatt 361 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg 421 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa 481 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg 541 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca 601 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa 661 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa 721 agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata 781 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag 841 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa 901 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa 961 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt 1021 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc 1081 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga 1141 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga 1201 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa 1261 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa 1321 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca 1381 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt 1441 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat 1501 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag 1561 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt 1621 atcgccgaat tctcaacaat aggcagtatt catccacaat tttaaaagaa aaggggggat 1681 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 1741 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattact cggacagcag 1801 agatccagtt tggactagtg gccgcaataa aatatcttta ttttcattac atctgtgtgt 1861 tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca 1921 aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctcta 1981 tcgataggta ccgagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata 2041 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 2101 aactcatcaa tgtatcttat catgtctgga tccccgggaa cagattcgcg actagtggac 2161 acacaggata ggggtgggca gcatgagggg ggccagaggg agagggggca gagctgggct 2221 ggacgagggg acaccgcccc caatggggcg tgacgagctg ccagcccagg ctccggggca 2281 cgtaaaagcg gggctgtgag accggagagc acggagcgac attaatatgg ccaagccttt 2341 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat 2401 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg 2461 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc 2521 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg 2581 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa 2641 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc 2701 ctctggttat gtgtgggagg gccccctctc cctccccccc ccctaacgtt actggccgaa 2761 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt 2821 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg 2881 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc 2941 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc 3001 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa 3061 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc 3121 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg 3181 gatctgatct ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac 3241 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg 3301 gccacaacca tagaccatgg ccgctggccg ccccgtgcgt gggcccgagc tggcgccccg 3361 gaggctgctg cagttgctgc tgctggtact gctggggggc ccgggccggg gggcggcctt 3421 gagcgggaac gtgaccgggc ctgggcctca cagcgccagc gggagctcga ggagggacgt 3481 gccggtgacc agccctccgc cgccgctgct gagccactgc ggccgggccg cccactgcga 3541 gcctttgcgc tacaacgtgt gcctgggctc ggcgctgccc tacggagcca ccaccacgct 3601 gctggctggg gactcggact cgcaggagga agcgcacggc aagctcgtgc tctggtccgg 3661 cctccggaat gccccccgct gctgggcagt gatccagccc ctgctgtgtg ctgtctacat 3721 gcccaagtgt gagaatgacc gagtggagtt gcccagccgt accctctgcc aggccacccg 3781 aggcccctgt gccattgtgg agcgggagcg agggtggcct gactttctgc gttgcacacc 3841 ggaccacttc cctgaaggct gcccaaacga ggtacaaaac atcaagttca acagctcagg 3901 ccaatgtgaa gcacccttgg tgcgaacaga caaccccaag agctggtatg aggacgtgga 3961 gggctgtggg attcagtgtc agaacccgct gttcaccgag gccgagcacc aggacatgca 4021 cagctacatc gcagccttcg gggcggtcac tggtctctgc acgctcttca cactggccac 4081 ctttgtggct gactggcgga actccaatcg ctaccctgcg gttattctct tctatgtcaa 4141 tgcgtgtttc ttcgtgggca gcattggctg gctggcccag ttcatggatg gtgcccgccg 4201 agagattgtt tgccgagcag atggcaccat gagatttggg gagcccacct ccagtgagac 4261 cctgtcctgt gtcatcatct ttgtcattgt gtactatgcc ttgatggctg gagtagtctg 4321 gttcgtggtc ctcacctatg cctggcacac ctccttcaaa gccctgggca ccacctacca 4381 gcctctctcg ggcaagacat cctatttcca cctgctcacg tggtcactcc cctttgtcct 4441 cacggtggca atcctggctg tggctcaggt agatggagac tccgtgagtg gcatctgttt 4501 tgtaggctac aagaactatc ggtaccgtgc tggctttgtc ctggccccaa ttggcctggt 4561 gcttattgtg ggaggctact tcctcatcag aggggtcatg actctgttct ccatcaagag 4621 caaccaccct gggcttctga gtgagaaggc agccagcaag atcaacgaga ccatgctgcg 4681 cctgggcatt tttggcttcc tggcctttgg ctttgtgctc atcaccttca gctgccactt 4741 ctatgacttc ttcaaccagg ctgagtggga gcgtagcttc cgggactatg tgctatgcca 4801 agccaacgtg accatcgggc tgcctaccaa gaagcccatt cctgactgtg agatcaagaa 4861 tcggcccagc ctcctggtgg agaagatcaa tctatttgcc atgtttggca ctggcattgc 4921 catgagcacc tgggtctgga ccaaggccac cctgctcatc tggaggcgca cctggtgcag 4981 gttgactggg cacagtgatg atgagcccaa gagaatcaag aagagcaaga tgatcgccaa 5041 ggccttctct aagcggcgtg agctgctgca gaacccgggc caggagctct ccttcagcat 5101 gcacactgtc tcccatgatg gacctgttgc gggtttggct tttgacctca atgaaccctc 5161 agctgacgtc tcctctgcct gggctcagca tgtcaccaag atggtggctc ggagaggagc 5221 catattgccc caggatgtgt ccgttacccc tgtggcaact ccagtgccac cagaagaaca 5281 agccaacatg tggctggttg aggcagagat ctccccagag ttagagaagc gtttgggccg 5341 gaagaaaaag cggaggaaga ggaagaagga ggtgtgcccc ttgaggccag cccctgagct 5401 tcaccactct gcccctgttc ctgccaccag tgcagttcct cggctgcctc agctgcctcg 5461 gcagaagtgc ctggtagctg caaacgcctg gggaacaggg gagtcctgcc gacagggagc 5521 ctggactcta gtctccaacc ccttctgccc agagcctagt ccccatcaag atccatttct 5581 ccctggtgcc tcagcccccc gggtctgggc tcagggccgc ctccaggggc tgggatccat 5641 tcattcccgc actaacctaa tggaggctga gatcttggat gcagactcgg acttcgccac 5701 catggactac aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga 5761 cgatgacaag gcggccgctt agtaagtaag atccgaactc gagagatcct aatcaacctc 5821 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 5881 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 5941 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 6001 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 6061 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 6121 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 6181 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg 6241 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 6301 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 6361 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg 6421 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg 6481 ctaattcact cccaacgaag acaagatcac ctgcaggaca ggcgcgccct gctttttgct 6541 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 6601 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 6661 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 6721 tctagcaccc gggcgattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt 6781 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg 6841 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 6901 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 6961 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 7021 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 7081 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 7141 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 7201 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 7261 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 7321 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 7381 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 7441 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 7501 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 7561 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 7621 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 7681 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 7741 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 7801 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 7861 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 7921 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 7981 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 8041 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 8101 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 8161 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 8221 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 8281 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 8341 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 8401 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 8461 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 8521 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 8581 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 8641 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 8701 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 8761 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 8821 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 8881 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc 8941 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 9001 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt 9061 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa 9121 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 9181 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact 9241 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg 9301 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa 9361 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga 9421 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca 9481 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag 9541 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg 9601 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg 9661 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg 9721 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg //