LOCUS       Exported                9770 bp ds-DNA     circular SYN 12-JUL-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Pazour Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 10, 2020 from SnapGene 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9770
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     complement(1..20)
                     /label=HIV_Rev
                     /note="color: black; sequence: gatctgagggctcgccactc"
     primer_bind     1..20
                     /label=HIV_For
                     /note="color: black; sequence: gagtggcgagccctcagatc"
     misc_feature    280..405
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    902..1135
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     primer_bind     1586..1604
                     /label=Phage_5'_promoter_seq
                     /note="color: black; sequence: cagatccattcgattagtg"
     misc_feature    1660..1777
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     1795..1855
                     /label=SV40polyA_For
                     /note="color: black; sequence: 
                     cagcagagatccagtttggactagtGGCCGCAATAAAATATCTTTATTTTCATTACATC
                     TG"
     polyA_signal    1826..1874
                     /note="synthetic polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    1888..1979
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
                     /note="color: #a6acb3"
     polyA_signal    1998..2132
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(2132..2175)
                     /label=SV40polyA_Rev
                     /note="color: black; sequence: 
                     acccctatcctgtgtgtccactagtCGCGAATCTGTTCCCGGGG"
     primer_bind     2133..2150
                     /label=Fragment.FOR
                     /note="color: black; sequence: 
                     tggaCTAGTCCAGTGTTTAATCCCGGGAACAGATTCGCG"
     primer_bind     2150..2177
                     /label=GgCry_For
                     /note="color: black; sequence: 
                     cagcagagatccagtttggactagtggacacacaggataggggtgg"
     promoter        2157..2320
                     /label=Chicken delta-1-crystallin gene, promoter region.
                     /note="color: #ffffff"
     primer_bind     2250..2264
                     /label=Cry_For_seq
                     /note="color: black; sequence: gtgacgagctgccag"
     primer_bind     complement(2296..2326)
                     /label=Smo_GgCryD1_Rev2
                     /note="color: black; sequence: 
                     gccatggtCtgatcattaatgtcgctccgtgctctccggtctcac"
     primer_bind     complement(2303..2320)
                     /label=GgCry_Rev
                     /note="color: black; sequence: 
                     GTCTTTGTAGTCCATGGTGGGATCCgtcgctccgtgctctccg"
     primer_bind     2307..2348
                     /label=BSD2_FOR
                     /note="color: black; sequence: 
                     gagcacggagcgacattaatatggccaagcctttgtctcaag"
     mRNA            2311..2320
                     /product="delta-1 crystallin mRNA"
                     /label=delta-1 crystallin mRNA
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             2327..2722
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /note="color: #ccffcc"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     2380..2403
                     /label=Sequences
                     /note="color: black; sequence: tacaatcaacagcatccccatctc"
     primer_bind     complement(2703..2732)
                     /label=BSD2_REV
                     /note="color: black; sequence: 
                     gggagagggggccctcccacacataaccag"
     primer_bind     complement(2703..2722)
                     /label=WPRE to IRES
                     /note="color: black; sequence: 
                     ccagaggttgattagGATCTCTCGAGttagccctcccacacataaccag"
     primer_bind     2713..2740
                     /label=Ires2_FOR
                     /note="color: black; sequence: 
                     gtgggagggccccctctccctccccccc"
     misc_feature    2723..3309
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (ECMV)"
                     /note="This feature has 3 segments:
                        1: 2723 .. 3297 / #a6acb3
                        2: 3298 .. 3300 / #a6acb3 / ATG
                        3: 3301 .. 3309 / #a6acb3"
     primer_bind     complement(3285..3331)
                     /label=Ires2_REV
                     /note="color: black; sequence: 
                     gcggccagcggccatggtCtatggttgtggccatattatcatcgtgt"
     primer_bind     3296..3330
                     /label=Smo_For_2nd
                     /note="color: black; sequence: 
                     ATATGGCCACAACCATAGACCATGGCCGCTGGCCG"
     primer_bind     3312..3327
                     /label=Smo_F2
                     /note="color: black; sequence: 
                     CGGCCttaattaatgatcaGaccatggccgctgg"
     gene            3315..5695
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /label=Smo
                     /note="smoothened, frizzled class receptor"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:319757"
                     /db_xref="MGI:MGI:108075"
     exon            3315..3659
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="color: #708090"
                     /inference="alignment:same species:1.39.8"
     CDS             3317..5695
                     /codon_start=1
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /product="smoothened homolog precursor"
                     /label=Smo
                     /note="bent body; smoothened homolog"
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS19965.1"
                     /db_xref="GeneID:319757"
                     /db_xref="MGI:MGI:108075"
                     /protein_id="NP_795970.3"
                     /translation="MAAGRPVRGPELAPRRLLQLLLLVLLGGPGRGAALSGNVTGPGPH
                     SASGSSRRDVPVTSPPPPLLSHCGRAAHCEPLRYNVCLGSALPYGATTTLLAGDSDSQE
                     EAHGKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVER
                     ERGWPDFLRCTPDHFPEGCPNEVQNIKFNSSGQCEAPLVRTDNPKSWYEDVEGCGIQCQ
                     NPLFTEAEHQDMHSYIAAFGAVTGLCTLFTLATFVADWRNSNRYPAVILFYVNACFFVG
                     SIGWLAQFMDGARREIVCRADGTMRFGEPTSSETLSCVIIFVIVYYALMAGVVWFVVLT
                     YAWHTSFKALGTTYQPLSGKTSYFHLLTWSLPFVLTVAILAVAQVDGDSVSGICFVGYK
                     NYRYRAGFVLAPIGLVLIVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETMLRLGI
                     FGFLAFGFVLITFSCHFYDFFNQAEWERSFRDYVLCQANVTIGLPTKKPIPDCEIKNRP
                     SLLVEKINLFAMFGTGIAMSTWVWTRATLLIWRRTWCRLTGHSDDEPRRIRRSRMIARA
                     FSRRRELLQNPGQELSFSMHTVSHDGPVAGLAFDLNEPSADVSSAWAQHVTRMVARRGA
                     ILPQDVSVTPVATPVPPEEQANMWLVEAEISPELERRLGRRRRRRRRRREVCPLRPAPE
                     LHHSAPVPATSAVPRLPQLPRQRCLVAANAWGTGESCRQGAWTLVSNPFCPEPSPHQDP
                     FLPGASAPRVWAQGRLQGLGSIHSRTNLMEAEILDADSDF"
     sig_peptide     3317..3412
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="color: #cc99b2; direction: RIGHT"
                     /inference="COORDINATES:same species:SignalP"
     mat_peptide     3413..5695
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /product="Smoothened homolog"
                     /label=propagated from UniProtKB/Swiss-Prot (P56726.2)
                     /note="propagated from UniProtKB/Swiss-Prot (P56726.2)"
                     /note="color: #cc99b2; direction: RIGHT"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            3660..3865
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="color: #708090"
                     /inference="alignment:same species:1.39.8"
     exon            3866..4075
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="color: #708090"
                     /inference="alignment:same species:1.39.8"
     misc_feature    4028..4090
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /label=Transmembrane 1
                     /note="propagated from UniProtKB/Swiss-Prot (P56726.2); 
                     transmembrane region"
                     /note="color: #a6acb3"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            4076..4248
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="color: #708090"
                     /inference="alignment:same species:1.39.8"
     misc_feature    4112..4174
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /label=Transmembrane 2
                     /note="propagated from UniProtKB/Swiss-Prot (P56726.2); 
                     transmembrane region"
                     /note="color: #a6acb3"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            4249..4468
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="color: #708090"
                     /inference="alignment:same species:1.39.8"
     misc_feature    4271..4333
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /label=Transmembrane 3
                     /note="propagated from UniProtKB/Swiss-Prot (P56726.2); 
                     transmembrane region"
                     /note="color: #a6acb3"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     primer_bind     complement(4343..4389)
                     /label=MmSmo_loop2_R
                     /note="color: black; sequence: 
                     gagagaggctggtaggtggtgcccagggctctgaaggaggtgtgcca"
     primer_bind     4369..4419
                     /label=MmSmo_loop2_F
                     /note="color: black; sequence: 
                     caccacctaccagcctctctcgggcaggacatcctatttccacctgctcac"
     misc_feature    4403..4465
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /label=Transmembrane 4
                     /note="propagated from UniProtKB/Swiss-Prot (P56726.2); 
                     transmembrane region"
                     /note="color: #a6acb3"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            4469..4592
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="color: #708090"
                     /inference="alignment:same species:1.39.8"
     misc_feature    4535..4597
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /label=Transmembrane 5
                     /note="propagated from UniProtKB/Swiss-Prot (P56726.2); 
                     transmembrane region"
                     /note="color: #a6acb3"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            4593..4685
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="color: #708090"
                     /inference="alignment:same species:1.39.8"
     primer_bind     complement(4597..4641)
                     /label=MmSmo_Loop3_R
                     /note="color: black; sequence: 
                     ctcagaagcccagggtggttgctcctgatggagaacagagtcatg"
     primer_bind     4621..4680
                     /label=MmSmo_loop3_F
                     /note="color: black; sequence: 
                     caaccaccctgggcttctgagtgagagggcagccagcaggatcaacgagaccatgctgc
                     g"
     misc_feature    4682..4744
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /label=Transmembrane 6
                     /note="propagated from UniProtKB/Swiss-Prot (P56726.2); 
                     transmembrane region"
                     /note="color: #a6acb3"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            4686..4794
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="color: #708090"
                     /inference="alignment:same species:1.39.8"
     exon            4795..4980
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="color: #708090"
                     /inference="alignment:same species:1.39.8"
     primer_bind     complement(4881..4921)
                     /label=MmSmo_Y
                     /note="color: black; sequence: 
                     ggcaatgccagtgccaaacatggcaaatagattgatcttct"
     misc_feature    4901..4963
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /label=Transmembrane 7
                     /note="propagated from UniProtKB/Swiss-Prot (P56726.2); 
                     transmembrane region"
                     /note="color: #a6acb3"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     primer_bind     4901..4921
                     /label=MmSmo_cterm_F
                     /note="color: black; sequence: atgtttggcactggcattgcc"
     misc_feature    4940..5035
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="propagated from UniProtKB/Swiss-Prot (P56726.2); 
                     Region: Interaction with BBS5 and BBS7"
                     /note="color: #a6acb3"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    4943..4945
                     /label=mutation
                     /note="color: #a6acb3"
     exon            4981..5129
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="color: #708090"
                     /inference="alignment:same species:1.39.8"
     misc_feature    5009..5011
                     /label=mutation
                     /note="color: #a6acb3"
     misc_feature    5018..5023
                     /label=mutation
                     /note="color: #a6acb3"
     misc_feature    5027..5029
                     /label=mutation
                     /note="color: #a6acb3"
     misc_feature    5039..5041
                     /label=mutation
                     /note="color: #a6acb3"
     misc_feature    5051..5053
                     /label=mutation
                     /note="color: #a6acb3"
     exon            5130..5264
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="color: #708090"
                     /inference="alignment:same species:1.39.8"
     misc_feature    5198..5200
                     /label=mutation
                     /note="color: #a6acb3"
     exon            5265..5695
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="color: #708090"
                     /inference="alignment:same species:1.39.8"
     misc_feature    5327..5329
                     /label=mutation
                     /note="color: #a6acb3"
     misc_feature    5342..5350
                     /label=mutation
                     /note="color: #a6acb3"
     STS             5347..5695
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /standard_name="Smo"
                     /label=Smo
                     /note="color: #a6acb3"
                     /db_xref="UniSTS:546036"
     misc_feature    5357..5359
                     /label=mutation
                     /note="color: #a6acb3"
     misc_feature    5363..5368
                     /label=mutation
                     /note="color: #a6acb3"
     misc_feature    5465..5467
                     /label=mutation
                     /note="color: #993366"
     primer_bind     complement(5671..5695)
                     /label=MmSmo_cterm_R
                     /note="color: black; sequence: gaagtccgagtctgcatccaagatc"
     primer_bind     complement(5678..5716)
                     /label=Smo_R2_2nd
                     /note="color: black; sequence: 
                     GTCTTTGTAGTCCATGGTGGCGAAGTCCGAGTCTGCATC"
     primer_bind     5678..5716
                     /label=MmSmo_X
                     /note="color: black; sequence: 
                     gatgcagactcggacttcGCCACCATGGACTACAAAGAC"
     primer_bind     complement(5678..5716)
                     /label=Smo_R2
                     /note="color: black; sequence: 
                     GTCTTTGTAGTCCATGGTGGCgaagtccgagtctgcatc"
     CDS             5705..5770
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(5746..5795)
                     /label=Flag-Rev
                     /note="color: black; sequence: 
                     cGGATCttacttactAaGCGGCCGCCTTGTCATCGTCATCCTTGTAATCG"
     primer_bind     5746..5795
                     /label=IRES to WPRE
                     /note="color: black; sequence: 
                     CGATTACAAGGATGACGATGACAAGGCGGCCGCtTagtaagtaaGATCCg"
     primer_bind     5776..5823
                     /label=WPRE-For
                     /note="color: black; sequence: 
                     CGCtTagtaagtaaGATCCgaaCTCGAGAGATCctaatcaacctctgg"
     primer_bind     5796..5823
                     /label=Fragment 3.FOR
                     /note="color: black; sequence: 
                     ctggttatgtgtgggagggctaaCTCGAGAGATCctaatcaacctctgg"
     primer_bind     complement(5803..5823)
                     /label=Primer 1
                     /note="color: black; sequence: ccagaggttgattagGATCTC"
     misc_feature    5811..6399
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(6282..6293)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 6281"
                     /translation="IEGR"
     LTR             6547..6727
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     promoter        6860..6964
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6965..7825
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6965 .. 7033 / #ccffcc / signal sequence
                        2: 7034 .. 7825 / #ccffcc
                     Cleavage site after base 7033"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7317..7345)
                     /label=Greg_Amp_R
                     /note="color: black; sequence: 
                     CATGGTTATGGCAGCACTGCATAATTCTC"
     primer_bind     7317..7345
                     /label=Greg_Amp_F
                     /note="color: black; sequence: 
                     GAGAATTATGCAGTGCTGCCATAACCATG"
     primer_bind     complement(7318..7336)
                     /label=Fragment 3.REV
                     /note="color: black; sequence: GGCAGCACTGCATAATTCT"
     primer_bind     7318..7336
                     /label=Fragment 2.REV
                     /note="color: black; sequence: AGAATTATGCAGTGCTGCC"
     rep_origin      7996..8584
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     promoter        9031..9061
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 9031 .. 9036 / #ffffff / -35
                        2: 9037 .. 9054 / #ffffff
                        3: 9055 .. 9061 / #ffffff / -10"
     protein_bind    9069..9085
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     9076..9095
                     /label=M13-48REV
                     /note="color: black; sequence: CGGATAACAATTTCACACAG"
     primer_bind     9093..9109
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     9093..9109
                     /label=M13R
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     LTR             join(9370..9770,1..233)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
ORIGIN
        1 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
       61 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      121 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      181 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      241 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      301 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tgcagagtac gccaaaaatt
      361 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      421 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      481 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      541 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      601 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      661 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      721 agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata
      781 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
      841 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
      901 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
      961 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1021 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1081 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1141 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1201 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     1261 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     1321 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     1381 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     1441 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     1501 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     1561 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt
     1621 atcgccgaat tctcaacaat aggcagtatt catccacaat tttaaaagaa aaggggggat
     1681 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     1741 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattact cggacagcag
     1801 agatccagtt tggactagtg gccgcaataa aatatcttta ttttcattac atctgtgtgt
     1861 tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca
     1921 aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctcta
     1981 tcgataggta ccgagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     2041 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     2101 aactcatcaa tgtatcttat catgtctgga tccccgggaa cagattcgcg actagtggac
     2161 acacaggata ggggtgggca gcatgagggg ggccagaggg agagggggca gagctgggct
     2221 ggacgagggg acaccgcccc caatggggcg tgacgagctg ccagcccagg ctccggggca
     2281 cgtaaaagcg gggctgtgag accggagagc acggagcgac attaatatgg ccaagccttt
     2341 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
     2401 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
     2461 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
     2521 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
     2581 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
     2641 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
     2701 ctctggttat gtgtgggagg gccccctctc cctccccccc ccctaacgtt actggccgaa
     2761 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     2821 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     2881 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     2941 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     3001 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     3061 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     3121 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     3181 gatctgatct ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     3241 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
     3301 gccacaacca tagaccatgg ccgctggccg ccccgtgcgt gggcccgagc tggcgccccg
     3361 gaggctgctg cagttgctgc tgctggtact gctggggggc ccgggccggg gggcggcctt
     3421 gagcgggaac gtgaccgggc ctgggcctca cagcgccagc gggagctcga ggagggacgt
     3481 gccggtgacc agccctccgc cgccgctgct gagccactgc ggccgggccg cccactgcga
     3541 gcctttgcgc tacaacgtgt gcctgggctc ggcgctgccc tacggagcca ccaccacgct
     3601 gctggctggg gactcggact cgcaggagga agcgcacggc aagctcgtgc tctggtccgg
     3661 cctccggaat gccccccgct gctgggcagt gatccagccc ctgctgtgtg ctgtctacat
     3721 gcccaagtgt gagaatgacc gagtggagtt gcccagccgt accctctgcc aggccacccg
     3781 aggcccctgt gccattgtgg agcgggagcg agggtggcct gactttctgc gttgcacacc
     3841 ggaccacttc cctgaaggct gcccaaacga ggtacaaaac atcaagttca acagctcagg
     3901 ccaatgtgaa gcacccttgg tgcgaacaga caaccccaag agctggtatg aggacgtgga
     3961 gggctgtggg attcagtgtc agaacccgct gttcaccgag gccgagcacc aggacatgca
     4021 cagctacatc gcagccttcg gggcggtcac tggtctctgc acgctcttca cactggccac
     4081 ctttgtggct gactggcgga actccaatcg ctaccctgcg gttattctct tctatgtcaa
     4141 tgcgtgtttc ttcgtgggca gcattggctg gctggcccag ttcatggatg gtgcccgccg
     4201 agagattgtt tgccgagcag atggcaccat gagatttggg gagcccacct ccagtgagac
     4261 cctgtcctgt gtcatcatct ttgtcattgt gtactatgcc ttgatggctg gagtagtctg
     4321 gttcgtggtc ctcacctatg cctggcacac ctccttcaaa gccctgggca ccacctacca
     4381 gcctctctcg ggcaagacat cctatttcca cctgctcacg tggtcactcc cctttgtcct
     4441 cacggtggca atcctggctg tggctcaggt agatggagac tccgtgagtg gcatctgttt
     4501 tgtaggctac aagaactatc ggtaccgtgc tggctttgtc ctggccccaa ttggcctggt
     4561 gcttattgtg ggaggctact tcctcatcag aggggtcatg actctgttct ccatcaagag
     4621 caaccaccct gggcttctga gtgagaaggc agccagcaag atcaacgaga ccatgctgcg
     4681 cctgggcatt tttggcttcc tggcctttgg ctttgtgctc atcaccttca gctgccactt
     4741 ctatgacttc ttcaaccagg ctgagtggga gcgtagcttc cgggactatg tgctatgcca
     4801 agccaacgtg accatcgggc tgcctaccaa gaagcccatt cctgactgtg agatcaagaa
     4861 tcggcccagc ctcctggtgg agaagatcaa tctatttgcc atgtttggca ctggcattgc
     4921 catgagcacc tgggtctgga ccagggccac cctgctcatc tggaggcgca cctggtgcag
     4981 gttgactggg cacagtgatg atgagcccag gagaatcagg aggagcagga tgatcgccag
     5041 ggccttctct aggcggcgtg agctgctgca gaacccgggc caggagctct ccttcagcat
     5101 gcacactgtc tcccatgatg gacctgttgc gggtttggct tttgacctca atgaaccctc
     5161 agctgacgtc tcctctgcct gggctcagca tgtcaccagg atggtggctc ggagaggagc
     5221 catattgccc caggatgtgt ccgttacccc tgtggcaact ccagtgccac cagaagaaca
     5281 agccaacatg tggctggttg aggcagagat ctccccagag ttagagaggc gtttgggccg
     5341 gaggagaagg cggaggagga ggaggaggga ggtgtgcccc ttgaggccag cccctgagct
     5401 tcaccactct gcccctgttc ctgccaccag tgcagttcct cggctgcctc agctgcctcg
     5461 gcagaggtgc ctggtagctg caaacgcctg gggaacaggg gagtcctgcc gacagggagc
     5521 ctggactcta gtctccaacc ccttctgccc agagcctagt ccccatcaag atccatttct
     5581 ccctggtgcc tcagcccccc gggtctgggc tcagggccgc ctccaggggc tgggatccat
     5641 tcattcccgc actaacctaa tggaggctga gatcttggat gcagactcgg acttcgccac
     5701 catggactac aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga
     5761 cgatgacaag gcggccgctt agtaagtaag atccgaactc gagagatcct aatcaacctc
     5821 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5881 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5941 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6001 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6061 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6121 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6181 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     6241 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6301 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6361 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg
     6421 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     6481 ctaattcact cccaacgaag acaagatcac ctgcaggaca ggcgcgccct gctttttgct
     6541 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6601 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6661 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6721 tctagcaccc gggcgattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
     6781 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     6841 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6901 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6961 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7021 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7081 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7141 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7201 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7261 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7321 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7381 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7441 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7501 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7561 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7621 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7681 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7741 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7801 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7861 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7921 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7981 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8041 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8101 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8161 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8221 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8281 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8341 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8401 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8461 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8521 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8581 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8641 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8701 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8761 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     8821 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     8881 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     8941 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     9001 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     9061 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     9121 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     9181 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     9241 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     9301 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     9361 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     9421 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     9481 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     9541 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     9601 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     9661 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     9721 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
//
