LOCUS       Exported                6712 bp ds-DNA     circular SYN 14-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 14, 2020 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     unsure          1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     promoter        156..597
                     /label=synapsin
     gene            598..2814
                     /label=eCB2.0
                     /label=GeCB2.0
     unsure          2829..3437
                     /label=WPRE
                     /note="/vntifkey=21"
     polyA_signal    3457..3935
                     /label=hGH polyA
                     /label=hGH polyA
                     /label=PolyA signal
                     /note="/vntifkey=25"
     unsure          3975..4115
                     /label=R-ITR
                     /note="/vntifkey=21"
     rep_origin      4207..4513
                     /label=f1 Origin
                     /label=f1 Origin
                     /label=Replication origin
                     /note="/vntifkey=33"
     unsure          5032..5889
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      6040..6707
                     /label=pUC ori
                     /label=pUC ori
                     /label=Replication origin
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatgag gcggggtggg ggtgcctacc
      181 tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc
      241 ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt
      301 cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca
      361 ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg
      421 gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg
      481 ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg
      541 tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg tgccaccatg
      601 gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac
      661 acaagtttgt acaaaaaagt tggcaccaag tcgatcctag atggccttgc agataccacc
      721 ttccgcacca tcaccactga cctcctgtac gtgggctcaa atgacattca gtacgaagac
      781 atcaaaggtg acatggcatc caaattaggg tacttcccac agaaattccc tttaacttcc
      841 tttaggggaa gtcccttcca agagaagatg actgcgggag acaaccccca gctagtccca
      901 gcagaccagg tgaacattac agaattttac aacaagtctc tctcgtcctt caaggagaat
      961 gaggagaaca tccagtgtgg ggagaacttc atggacatag agtgtttcat ggtcctgaac
     1021 cccagccagc agctggccat tgcagtcctg tccctcacgc tgggcacctt cacggtcctg
     1081 gagaacctcc tggtgctgtg cgtcatcctc cactcccgca gcctccgctg caggccttcc
     1141 taccacttca tcggcagcct ggcggtggca gacctcctgg ggagtgtcat ttttgtctac
     1201 agcttcattg acttccacgt gttccaccgc aaagatagcc gcaacgtgtt tctgttcaaa
     1261 ctgggtgggg tcacggcctc cttcactgcc tccgtgggca gcctgttcct cacagccatc
     1321 gacaggtaca tatccattca caggcccctg gcctataaga ggattgtcac caggcccaag
     1381 gccgtggtgg cgttttgcct gatgtggacc atagccattg tgatcgccgt gctgcctctc
     1441 ctgggctgga actgcgagaa actgcaatct gtttgctcag acattttccc acacattgat
     1501 gaaacctacc tgatgttctg gatcggggtc accagcgtac tgcttctgtt catcgtgtat
     1561 gcgtacatgt atattctctg gaaggctcac agccacgccg tccgcatgat tcagcgtacc
     1621 gacgcgctgg acctggagga gggaggaaac gtctatatca aggccgacaa gcagaagaac
     1681 ggcatcaagg cgaacttctg catccgccac aacatcgagg acggcggcgt gcagctcgcc
     1741 taccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1801 tacctgagcg tgcagtccaa actttcgaaa gaccccaacg agaagcgcga tcacatggtc
     1861 ctgctggagt tcgtgaccgc cgccgggatc actttcggca tggacgagct gtacaagggc
     1921 ggtaccggag ggagcatggt gagaaagggc gaggagctgt tcaccggggt ggtgcccatc
     1981 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgggcgg cgagggtgag
     2041 ggcgatgcca ccgttggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     2101 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     2161 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacatccag
     2221 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     2281 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     2341 aacatcctgg ggcacaagct ggagtacaac accggagcag cagcacgctg gcgcgggcgg
     2401 cgcatggaca ttaggttagc caagaccctg gtcctgatcc tggtggtgtt gatcatctgc
     2461 tggggccctc tgcttgcaat catggtgtat gatgtctttg ggaagatgaa caagctcatt
     2521 aagacggtgt ttgcattctg caccatgctc tgcctgctga actccaccgt gaaccccatc
     2581 atctatgctc tgaggagtaa ggacctgcga cacgctttcc ggagcatgtt tccctcttgt
     2641 gaaggcactg cgcagcctct ggataacagc atgggggact cggactgcct gcacaaacac
     2701 gcaaacaatg cagccagtgt tcacagggcc gcagaaagct gcatcaagag cacggtcaag
     2761 attgccaagg taaccatgtc tgtgtccaca gacacgtctg ccgaggctct gtgagaattc
     2821 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2881 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2941 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3001 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3061 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3121 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3181 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     3241 tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc
     3301 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3361 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3421 tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc tgtgacccct
     3481 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
     3541 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
     3601 ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct
     3661 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
     3721 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
     3781 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
     3841 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
     3901 tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta accacgtgcg
     3961 gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     4021 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     4081 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     4141 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     4201 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4261 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4321 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4381 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     4441 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4501 caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg
     4561 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4621 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     4681 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     4741 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4801 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     4861 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4921 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4981 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5041 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5101 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5161 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5221 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5281 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5341 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5401 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5461 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5521 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5581 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5641 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5701 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5761 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5821 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5881 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5941 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6001 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6061 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6121 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6181 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     6241 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6301 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6361 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6421 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6481 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6541 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6601 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6661 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
