LOCUS       Exported                6769 bp ds-DNA     circular SYN 14-DEC-2020
DEFINITION  Sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 14, 2020 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        163..604
                     /label=hsyn promoter
                     /label=hsyn
                     /label=hsyn promoter
     primer_bind     623..654
                     /label=Loxp
     misc_feature    707..740
                     /label=loxP
                     /label=loxP
                     /label=loxP
                     /label=loxP
                     /label=loxP
     gene            complement(751..2967)
                     /label=eCBmut
     gene            complement(2968..2973)
                     /label=Kozak sequence
                     /label=Kozak sequence
                     /label=Kozak sequence
     primer_bind     2982..3013
                     /label=Loxp
     primer_bind     3064..3097
                     /label=Loxp
     misc_feature    3124..3486
                     /label=WPRE
                     /label=WPRE
                     /label=WPRE
                     /label=WPRE
                     /label=WPRE
     terminator      3515..3993
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
     rep_origin      4265..4571
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
     promoter        5020..5048
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
     gene            5090..5950
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
     rep_origin      6105..6724
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
ORIGIN
        1 tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt
       61 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
      121 aactccatca ctaggggttc ctgcggccgc acgcgtaagc ttatgaggcg gggtgggggt
      181 gcctacctga cgaccgaccc cgacccactg gacaagcacc caacccccat tccccaaatt
      241 gcgcatcccc tatcagagag ggggagggga aacaggatgc ggcgaggcgc gtgcgcactg
      301 ccagcttcag caccgcggac agtgccttcg cccccgcctg gcggcgcgcg ccaccgccgc
      361 ctcagcactg aaggcgcgct gacgtcactc gccggtcccc cgcaaactcc ccttcccggc
      421 caccttggtc gcgtccgcgc cgccgccggc ccagccggac cgcaccacgc gaggcgcgag
      481 ataggggggc acgggcgcga ccatctgcgc tgcggcgccg gcgactcagc gctgcctcag
      541 tctgcggtgg gcagcggagg agtcgtgtcg tgcctgagag cgcagtcgag aaaccggtgc
      601 cacctctaga gtcgactccg gaataacttc gtataggata ctttatacga agttatgcag
      661 aatggtagct ggattgtagc tgctattagc aatatgaaac ctcttaataa cttcgtatag
      721 catacattat acgaagttat ggcgcatatg tcacagagcc tcggcagacg tgtctgtgga
      781 cacagacatg gttaccttgg caatcttgac cgtgctcttg atgcagcttt ctgcggccct
      841 gtgaacactg gctgcattgt ttgcgtgttt gtgcaggcag tccgagtccc ccatgctgtt
      901 atccagaggc tgcgcagtgc cttcacaaga gggaaacatg ctccggaaag cgtgtcgcag
      961 gtccttactc ctcagagcat agatgatggg gttcacggtg gagttcagca ggcagagcat
     1021 ggtgcagaat gcaaacaccg tcttaatgag cttgttcatc ttcccaaaga catcatacac
     1081 catgattgca agcagagggc cccagcagat gatcaacacc accaggatca ggaccagggt
     1141 cttggctaac ctaatgtcca tgcgccgccc gcgccagcgt gctgctgctc cggtgttgta
     1201 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
     1261 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
     1321 gtccttgaag aagatggtgc gctcctggat gtagccttcg ggcatggcgg acttgaagaa
     1381 gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt
     1441 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
     1501 cagcttgcca acggtggcat cgccctcacc ctcgccgccc acgctgaact tgtggccgtt
     1561 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
     1621 tctcaccatg ctccctccgg taccgccctt gtacagctcg tccatgccga aagtgatccc
     1681 ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttcga
     1741 aagtttggac tgcacgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat
     1801 gggggtgttc tgctggtagt ggtaggcgag ctgcacgccg ccgtcctcga tgttgtggcg
     1861 gatgcagaag ttcgccttga tgccgttctt ctgcttgtcg gccttgatat agacgtttcc
     1921 tccctcctcc aggtccagcg cgtcggtacg ctgaatcatg cggacggcgt ggctgtgagc
     1981 cttccagaga atatacatgt acgcatacac gatgaacaga agcagtacgc tggtgacccc
     2041 gatccagaac atcaggtagg tttcatcaat gtgtgggaaa atgtctgagc aaacagattg
     2101 cagtttctcg cagttccagc ccaggagagg cagcacggcg atcacaatgg ctatggtcca
     2161 catcaggcaa aacgccacca cggccttggg cctggtgaca atcctcttat aggccagggg
     2221 cctgtgaatg gatatgtacc tgtcgatggc tgtgaggaac aggctgccca cggaggcagt
     2281 gaaggaggcc gtgaccccac ccagtttgaa cagaaacacg ttgcggctat ctttgcggtg
     2341 gaacacgtgg gcgtcaatga agctgtagac aaaaatgaca ctccccagga ggtctgccac
     2401 cgccaggctg ccgatgaagt ggtaggaagg cctgcagcgg aggctgcggg agtggaggat
     2461 gacgcacagc accaggaggt tctccaggac cgtgaaggtg cccagcgtga gggacaggac
     2521 tgcaatggcc agctgctggc tggggttcag gaccatgaaa cactctatgt ccatgaagtt
     2581 ctccccacac tggatgttct cctcattctc cttgaaggac gagagagact tgttgtaaaa
     2641 ttctgtaatg ttcacctggt ctgctgggac tagctggggg ttgtctcccg cagtcatctt
     2701 ctcttggaag ggacttcccc taaaggaagt taaagggaat ttctgtggga agtaccctaa
     2761 tttggatgcc atgtcacctt tgatgtcttc gtactgaatg tcatttgagc ccacgtacag
     2821 gaggtcagtg gtgatggtgc ggaaggtggt atctgcaagg ccatctagga tcgacttggt
     2881 gccaactttt ttgtacaaac ttgtgtcacc agtggaacct ggaacccaga gcagcagtac
     2941 ccatagcagg agtgtgtctg tctccatggt ggcgctagca taacttcgta taaagtatcc
     3001 tatacgaagt tatttgcctt aacccagaaa ttatcactgt tattctttag aatggtgcaa
     3061 agaataactt cgtataatgt atgctatacg aagttatgaa ttcgatatca agcttatcga
     3121 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     3181 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     3241 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     3301 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     3361 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     3421 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3481 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     3541 cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     3601 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     3661 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     3721 agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     3781 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     3841 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     3901 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     3961 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     4021 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     4081 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     4141 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     4201 gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg
     4261 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     4321 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     4381 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4441 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     4501 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4561 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4621 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4681 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4741 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4801 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4861 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4921 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4981 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5041 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     5101 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     5161 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5221 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5281 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5341 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5401 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5461 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5521 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5581 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5641 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5701 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5761 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5821 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5881 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5941 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6001 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6061 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6121 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6181 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6241 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6301 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6361 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6421 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6481 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6541 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6601 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6661 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6721 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggaga
//
