LOCUS       pCMV-sypHTomato         6777 bp ds-DNA     circular SYN 02-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    a2A-A2A-pDisplay-IRES-mCherry-b
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct
            synthetic DNA construct
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, November 8, 2017 from SnapGene Viewer 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     promoter        390..593
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        638..656
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             739..2508
                     /label=sypHTomato
     gene            1318..2022
                     /label=pHTomato
     gene            2479..2505
                     /label="HA tag"
     polyA_signal    2533..2757
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2889..3477)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    3806..3853
                     /note="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(4908..5268)
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      4922..5060
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             complement(5328..6188)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6189..6293)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      6320..6775
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 GCGCGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG
       61 TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT
      121 GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC
      181 CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGACTATTT ACGGTAAACT GCCCACTTGG
      241 CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT
      301 GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA
      361 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC
      421 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA
      481 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT
      541 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC
      601 TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA
      661 CCCAAGCTTG GTACCGAGCT CGGATCCACT AGTAACGGCC GCCAGTGTGC TGGAATTCGG
      721 CTTGGGGATA TCGCCACCAT GGACGTGGTG AATCAGCTGG TGGCTGGGGG TCAGTTCCGG
      781 GTGGTCAAGG AGCCCCTTGG CTTCGTGAAG GTGCTGCAGT GGGTCTTTGC CATCTTCGCC
      841 TTTGCTACGT GCGGCAGCTA CACCGGAGAG CTTCGGCTGA GCGTGGAGTG TGCCAACAAG
      901 ACGGAGAGTG CCCTCAACAT CGAAGTCGAA TTTGAGTACC CATTCAGGCT GCACCAAGTG
      961 TACTTTGATG CACCCTCCTG CGTTAAAGGG GGCACTACCA AGATCTTCCT AGTTGGTGAC
     1021 TACTCCTCCT CGGCTGAATT CTTTGTCACC GTGGCTGTGT TTGCCTTCCT CTACTCCATG
     1081 GGGGCCCTGG CCACCTACAT CTTCCTGCAG AACAAGTACC GAGAGAACAA CAAAGGGCCA
     1141 ATGATGGACT TCCTGGCCAC AGCAGTGTTC GCTTTCATGT GGCTAGTTAG CTCATCCGCC
     1201 TGGGCCAAAG GCCTGTCCGA TGTGAAGATG GCCACTGACC CAGAGAACAT TATCAAGGAG
     1261 ATGCCTATGT GCCGCCAGAC AGATCTAGCC ACCGGATCCA CAGGTGGAGC CTCCTCCGTG
     1321 AGCAAGGGCG AGGAGAATAA CATGGCCATC ATCAAGGAGT TCATGCGCTT CAAGGTGCGC
     1381 ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC
     1441 TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG GCGGCCCCCT GCCCTTCGCC
     1501 TGGGACATCC TGTCCCCTCA GTTCACGTAC GGCTCCAAGG CCTACGTGAA GCACCCCGCC
     1561 GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG
     1621 AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCGAG
     1681 TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT CCGACGGCCC CGTAATGCAG
     1741 AAGAAGACCA TGGGCTGGGA GGCCTCCACC GAGCGGATGT ACCCCGAGGA CGGCGCCCTG
     1801 AAGGGCGAGA TCAAGATGAG GCTGAAGCTG AAGGACGGCG GCCACTACGA CGCCGAGGTC
     1861 AAGACCACCT ACATGGCCAA GAAGCCCGTG CAGCTGCCCG GCGCCTACAA GACCGACATC
     1921 AAGCTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG TGGAACAGTA CGAGCGCGCC
     1981 GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA AGTCCACCGG CGCCAGCGGA
     2041 GGCACAGGTT CAGGTACCGG TGGAGCTCGA ATCACATGCA AGGAACTGAG GGACCCTGTG
     2101 ACTTCAGGAC TCAACACCTC GGTGGTGTTT GGCTTCCTGA ACCTGGTGCT CTGGGTTGGC
     2161 AACCTATGGT TCGTGTTCAA GGAGACAGGC TGGGCCGCCC CATTCATGCG CGCACCTCCA
     2221 GGCGCCCCAG AAAAGCAACC AGCTCCTGGC GATGCCTACG GCGATGCGGG CTATGGGCAG
     2281 GGCCCCGGAG GCTATGGGCC CCAGGACTCC TACGGGCCTC AGGGTGGTTA TCAACCCGAT
     2341 TACGGGCAGC CAGCCAGCGG CGGTGGCGGT GGCTACGGGC CTCAGGGCGA CTATGGGCAG
     2401 CAAGGCTACG GCCAACAGGG TGCGCCCACC TCCTTCTCCA ATCAGATGTC GGATCCGAAG
     2461 CTTCGGTACC AAGCTGCCTA CCCTTACGAT GTGCCCGATT ACGCCTGAGC GGCCGCTCGA
     2521 GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC
     2581 CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG
     2641 CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA
     2701 AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT
     2761 CTGAGGCGGA AAGAACCAGT GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA
     2821 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG
     2881 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT
     2941 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC
     3001 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT
     3061 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC
     3121 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA
     3181 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA
     3241 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG
     3301 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG
     3361 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT
     3421 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA
     3481 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     3541 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     3601 AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC
     3661 CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG
     3721 GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG
     3781 CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT
     3841 CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT
     3901 AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC
     3961 CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA
     4021 TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC
     4081 CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG
     4141 CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT
     4201 CACGGGTAGC CAACGCTATG TCCTGATAGC GGTCCGCCAC ACCCAGCCGG CCACAGTCGA
     4261 TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG
     4321 TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG
     4381 GCGCGAGCCC CTGATGCTCT TGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC
     4441 GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG
     4501 TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG
     4561 ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG
     4621 TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG
     4681 CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG
     4741 GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG
     4801 CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT
     4861 CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT
     4921 AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA GGAGGCGGCC
     4981 TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG
     5041 GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG
     5101 AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT
     5161 GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT
     5221 CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT
     5281 TTCAATAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     5341 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     5401 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     5461 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     5521 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     5581 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     5641 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     5701 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     5761 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     5821 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     5881 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     5941 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     6001 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     6061 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     6121 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     6181 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     6241 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT
     6301 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG
     6361 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC
     6421 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC
     6481 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT
     6541 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG
     6601 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG
     6661 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG
     6721 CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGCTTAC AATTTAC
//
