LOCUS       Exported               10126 bp ds-DNA     circular SYN 08-JUN-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 14, 2020 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10126
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             905..1621
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1:  905 ..  907 / #05fd14
                        2:  908 ..  910 / #05fd14 / 1a
                        3:  911 .. 1621 / #05fd14"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     protein_bind    1643..1667
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     1682..1705
                     /label=Insert.FOR
                     /note="color: black; sequence: atggagctggaggacggtgtggtg"
     primer_bind     complement(5621..5647)
                     /label=Insert.REV
                     /note="color: black; sequence: tcactcattgccatacatcacttgcca"
     protein_bind    complement(5661..5685)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     polyA_signal    5723..5947
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      5993..6421
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        6435..6764
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      6615..6750
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             6831..7625
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    7799..7920
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(7969..7985)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    7993..8009
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(8017..8047)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 8017 .. 8023 / #ffffff / -10
                        2: 8024 .. 8041 / #ffffff
                        3: 8042 .. 8047 / #ffffff / -35"
     protein_bind    8062..8083
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(8371..8959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(9130..9990)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 9130 .. 9921 / #ccffcc
                        2: 9922 .. 9990 / #ccffcc / signal sequence
                     Cleavage site after base 9921"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9991..10095)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctaga
      901 caccatggcc agcaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      961 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgctac
     1021 atacggaaag cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     1081 aacacttgtc actactttct cttatggtgt tcaatgcttt tcccgttatc cggatcatat
     1141 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat
     1201 atctttcaaa gatgacggga actacaagac gcgtgctgaa gtcaagtttg aaggtgatac
     1261 ccttgttaat cgtatcgagt taaaaggtat tgattttaaa gaagatggaa acattctcgg
     1321 acacaaactc gagtacaact ataactcaca caatgtatac atcacggcag acaaacaaaa
     1381 gaatggaatc aaagctaact tcaaaattcg ccacaacatt gaagatggat ccgttcaact
     1441 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     1501 ccattacctg tcgacacaat ctgccctttc gaaagatccc aacgaaaagc gtgaccacat
     1561 ggtccttctt gagtttgtaa ctgctgctgg gattacacat ggcatggatg agctctacaa
     1621 aagcggttgg cctccggatc aaacaagttt gtacaaaaaa gcaggctccg aattcgccct
     1681 tatggagctg gaggacggtg tggtgtatca ggaggagccc ggcggctccg gggccgtgat
     1741 gtcggagcgg gtgtccggcc tggccggctc catctaccgc gagttcgagc ggcttatcgg
     1801 gcgctatgac gaggaggtgg tcaaagagct gatgccgctg gtggtggctg tgctggagaa
     1861 cctggactcg gtgttcgcgc aggaccagga gcaccaggtg gagctggagc tgctgcggga
     1921 cgacaacgag cagctcatca cccagtacga gcgggagaag gcgctgcgca agcacgctga
     1981 ggagaaattc attgaatttg aagactctca agaacaggaa aaaaaggact tacagacccg
     2041 agtggaatct ttagaatctc aaacaagaca acttgagctg aaagcgaaaa actatgctga
     2101 ccagattagc agacttgaag aaagagaagc agaactgaag aaggaatata atgcattaca
     2161 tcaaagacac actgagatga tccataatta tatggaacat ttagaaagaa caaaacttca
     2221 tcagctctca gggagtgatc aactagaatc cacagctcat agtagaatta gaaaagaacg
     2281 ccctatatca ttaggaattt tcccattacc tgctggagat ggattgctta cacctgatgc
     2341 tcagaaagga ggagagaccc ctggatctga gcaatggaaa tttcaggaat taagtcaacc
     2401 acgttctcat accagcctga aggtcagcaa tagtcctgaa cctcagaagg ctgtagaaca
     2461 ggaggatgag ctttctgatg ttagccaagg cggatctaaa gctaccactc cagcatcaac
     2521 agctaattca gatgtggcaa caattcctac tgatactccc ttaaaggaag aaaacgaagg
     2581 atttgtgaag gttacagatg cgccaaataa atcagagata agcaaacaca ttgaagtaca
     2641 ggtagcccag gaaactagaa atgtatctac tggctctgct gaaaatgaag aaaagtcaga
     2701 agttcaagca atcatcgaat ctactcctga gctggatatg gacaaagatc tcagtggata
     2761 taaaggttca agcactccca ccaaaggcat agagaacaaa gcttttgatc gcaatacaga
     2821 atctctcttt gaagaactgt cttcagctgg ctcaggccta ataggagatg tggatgaagg
     2881 agcagattta ctaggaatgg gtcgggaagt tgagaatctt atattagaaa atacacaact
     2941 gttggaaacc aaaaatgctt tgaacatagt gaagaatgat ttgatagcaa aagtggatga
     3001 actgacctgt gagaaagatg tgctgcaagg ggaattggag gctgtgaagc aagccaaact
     3061 gaaactagag gaaaagaaca gagaattgga ggaagagctt aggaaagctc gggcagaagc
     3121 tgaagatgca aggcaaaaag caaaagatga cgatgatagt gatattccca cagcccagag
     3181 gaaacggttt actagagtag aaatggcccg tgttctcatg gagcgaaacc agtataaaga
     3241 gagattgatg gagcttcagg aagctgttcg atggacagag atgattcggg catcacgaga
     3301 aaatccagcc atgcaggaaa aaaaaaggtc aagcatttgg cagtttttca gccgactttt
     3361 cagctcctca agtaacacga ctaagaagcc tgaaccacct gttaatctga agtacaatgc
     3421 acccacgtct catgttactc cgtccgtcaa gaaaagaagc agcaccttat ctcagctccc
     3481 tggggataag tccaaagcct ttgatttcct tagtgaagaa actgaagcta gtttagcctc
     3541 acgcagagaa caaaagagag agcagtatcg tcaggtaaaa gcacatgttc agaaggaaga
     3601 cggtagagtg caggcttttg gctggagtct gcctcagaag tacaaacagg taaccaatgg
     3661 tcaaggtgaa aataagatga aaaatttacc tgtgcctgtc tatctcagac ctctggatga
     3721 aaaagataca tcaatgaagc tgtggtgtgc tgttggagtc aatttatctg gtgggaagac
     3781 cagagatggt ggttctgttg ttggagcaag tgtattttac aaggatgttg ctggtttgga
     3841 tacagaaggc agtaaacagc gaagtgcctc tcagagtagt ttagataagt tagatcagga
     3901 acttaaggaa cagcagaagg agttaaaaaa tcaagaagaa ttatccagtc tagtttggat
     3961 ctgtaccagc actcattcgg ctacaaaagt tcttattatt gatgctgttc aacctggcaa
     4021 catcctagac agtttcactg tttgcaactc tcatgttctg tgcattgcaa gtgtgccagg
     4081 tgcacgagaa acagactacc ctgcaggaga agatctttca gaatctggtc aggtagacaa
     4141 agcatcttta tgtggaagta tgacaagcaa cagctcagca gagacagaca gcctgttagg
     4201 aggcatcaca gtggttggtt gttctgcaga aggtgtgacg ggagctgcca cttcccctag
     4261 tacaaatggt gcttctccag tgatggataa accaccagaa atggaagcag aaaatagtga
     4321 ggttgatgaa aatgttccaa cagcagaaga agcaactgaa gctacagaag ggaatgcggg
     4381 gtcagctgaa gacacagtgg acatctccca aactggcgtc tacacagagc atgtctttac
     4441 agatcctttg ggagttcaga tcccagaaga cctctcccca gtgtatcagt cgagcaatga
     4501 ctcagatgca tataaagatc aaatatcagt actgccaaat gaacaagact tggtgagaga
     4561 agaagcccag aaaatgagta gtcttttacc aactatgtgg cttggagctc aaaatggctg
     4621 tttgtatgtc cattcatctg tagcccagtg gaggaaatgt ctccattcca ttaaacttaa
     4681 agattcgatt ctcagtattg tacacgtgaa gggaatcgtg ttagtagccc tggctgacgg
     4741 cacccttgca atctttcaca gaggagtgga tgggcagtgg gatttgtcaa actatcacct
     4801 cttagacctt ggacggcctc atcattccat ccgttgcatg actgtggtac atgacaaagt
     4861 ctggtgtggc tataggaaca aaatctatgt ggtgcagcca aaggccatga aaatagagaa
     4921 atcttttgat gcacatccca ggaaggagag ccaagtgcga cagcttgcgt gggtggggga
     4981 tggcgtgtgg gtctccattc gcttggattc tacgctccgt ctctatcatg cacacactta
     5041 tcaacatcta caggatgtgg acattgagcc ttatgtaagc aaaatgttag gtactggaaa
     5101 actgggcttc tcttttgtga gaattacagc tcttatggtg tcttgtaatc gtttgtgggt
     5161 ggggacagga aatggtgtca ttatctccat cccattgaca gaaacaaata aaacctcagg
     5221 tgtaccagga aatcgtcctg gaagtgtaat ccgtgtatat ggtgatgaaa acagtgataa
     5281 agtgactcca gggacattta taccctattg ttcaatggca catgcacagc tttgcttcca
     5341 tgggcaccgg gatgctgtga aattctttgt ggcagtccca ggtcaagtca tcagcccaca
     5401 aagtagcagt agtggcacgg atctgacggg tgacaaagca gggccatctg cacaggagcc
     5461 tggtagtcag acgcccttga agtctatgct tgtcatcagt ggaggagagg gctacatcga
     5521 cttccgaatg ggtgatgaag gtggagaatc agaacttctt ggagaggatc ttccacttga
     5581 accttctgtc accaaagcag aaaggagtca cttgatagtg tggcaagtga tgtatggcaa
     5641 tgagtgaaag ggcgaattcg acccagcttt cttgtacaaa gtggtgataa ttaattaaga
     5701 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     5761 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     5821 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     5881 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     5941 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     6001 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6061 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6121 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6181 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6241 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6301 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6361 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6421 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     6481 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     6541 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     6601 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6661 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     6721 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     6781 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     6841 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     6901 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     6961 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     7021 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     7081 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     7141 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     7201 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     7261 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     7321 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     7381 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     7441 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     7501 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     7561 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     7621 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     7681 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     7741 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     7801 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7861 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7921 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     7981 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8041 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8101 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8161 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8221 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8281 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8341 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8401 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8461 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8521 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8581 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8641 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8701 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8761 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8821 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8881 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8941 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9001 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9061 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9121 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9181 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9241 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9301 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9361 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9421 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9481 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9541 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9601 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9661 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9721 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9781 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9841 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9901 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9961 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10021 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10081 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
