LOCUS       Exported               13136 bp ds-DNA     circular SYN 06-JUN-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 13136)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 14, 2020 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13136
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             931..996
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2
                     Cleavage site after base 996"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             997..1743
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /note="color: #993366"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     protein_bind    1783..1807
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     1822..1854
                     /label=Insert.FOR
                     /note="color: black; sequence: 
                     ATGGCTAGTGGCAGCTGTCAGGGGTGCGAAGAG"
     primer_bind     complement(9347..9405)
                     /label=Insert.REV
                     /note="color: black; sequence: 
                     TTACTCAACAGATGTTCGTCTCATTTTTTCAGCTAATTCTTTTCTCACTTCAATGTGTT
                     "
     protein_bind    complement(9419..9443)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     polyA_signal    9477..10099
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        10128..10457
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      10308..10443
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        complement(10496..10514)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(10528..10544)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    10552..10568
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(10576..10606)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 10576 .. 10582 / #ffffff / -10
                        2: 10583 .. 10600 / #ffffff
                        3: 10601 .. 10606 / #ffffff / -35"
     protein_bind    10621..10642
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(10930..11518)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(11689..12549)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 11689 .. 12480 / #ccffcc
                        2: 12481 .. 12549 / #ccffcc / signal sequence
                     Cleavage site after base 12480"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12550..12654)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      12681..13136
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa
      961 gatcatgaca tcgattacaa ggatgacgat gacaagggaa agtcttaccc aactgtgagt
     1021 gctgattacc aggacgccgt tgagaaggcg aagaagaagc tcagaggctt catcgctgag
     1081 aagagatgcg ctcctctaat gctccgtttg gcattccact ctgctggaac ctttgacaag
     1141 ggcacgaaga ccggtggacc cttcggaacc atcaagcacc ctgccgaact ggctcacagc
     1201 gctaacaacg gtcttgacat cgctgttagg cttttggagc cactcaaggc ggagttccct
     1261 attttgagct acgccgattt ctaccagttg gctggcgttg ttgccgttga ggtcacgggt
     1321 ggacctaagg ttccattcca ccctggaaga gaggacaagc ctgagccacc accagagggt
     1381 cgcttgcccg atcccactaa gggttctgac catttgagag atgtgtttgg caaagctatg
     1441 gggcttactg accaagatat cgttgctcta tctgggggtc acactattgg agctgcacac
     1501 aaggagcgtt ctggatttga gggtccctgg acctctaatc ctcttatttt cgacaactca
     1561 tacttcacgg agttgttgag tggtgagaag gaaggtctcc ttcagctacc ttctgacaag
     1621 gctcttttgt ctgaccctgt attccgccct ctcgttgaca aatatgcagc ggacgaagat
     1681 gccttctttg ctgattacgc tgaggctcac caaaagcttt ccgagcttgg gtttgctgat
     1741 gccaagcttg cggccgcgaa ttcatcgata gatctgatat caacaagttt gtacaaaaaa
     1801 gcaggctccg aattcgccct tatggctagt ggcagctgtc aggggtgcga agaggacgag
     1861 gaaactctga agaagttgat agtcaggctg aacaatgtcc aggaaggaaa acagatagaa
     1921 acgctggtcc aaatcctgga ggatctgctg gtgttcacgt actccgagcg cgcctccaag
     1981 ttatttcaag gcaaaaatat ccatgtgcct ctgttgatcg tcttggactc ctatatgaga
     2041 gtcgcgagtg tgcagcaggt gggttggtca cttctgtgca aattaataga agtctgtcca
     2101 ggtacaatgc aaagcttaat gggaccccag gatgttggaa atgattggga agtccttggt
     2161 gttcaccaat tgattcttaa aatgctaaca gttcataatg ccagtgtaaa cttgtcagtg
     2221 attggactga agaccttaga tctcctccta acttcaggta aaatcacctt gctgatattg
     2281 gatgaagaaa gtgatatttt catgttaatt tttgatgcca tgcactcatt tccagccaat
     2341 gatgaagtcc agaaacttgg atgcaaagct ttacatgtgc tgtttgagag agtctcagag
     2401 gagcaactga ctgaatttgt tgagaacaaa gattatatga tattgttaag tgcgttaaca
     2461 aattttaaag atgaagagga aattgtgctt catgtgctgc attgtttaca ttccctagcg
     2521 attccttgca ataatgtgga agtcctcatg agtggcaatg tcaggtgtta taatattgtg
     2581 gtggaagcta tgaaagcatt ccctatgagt gaaagaattc aagaagtgag ttgctgtttg
     2641 ctccataggc ttacattagg taattttttc aatatcctgg tattaaacga agtccatgag
     2701 tttgtggtga aagctgtgca gcagtaccca gagaatgcag cattgcagat ctcagcgctc
     2761 agctgtttgg ccctcctcac tgagactatt ttcttaaatc aagatttaga ggaaaagaat
     2821 gagaatcaag agaatgatga tgagggggaa gaagataaat tgttttggct ggaagcctgt
     2881 tacaaagcat taacgtggca tagaaagaac aagcacgtgc aggaggccgc atgctgggca
     2941 ctaaataatc tccttatgta ccaaaacagt ttacatgaga agattggaga tgaagatggc
     3001 catttcccag ctcataggga agtgatgctc tccatgctga tgcattcttc atcaaaggaa
     3061 gttttccagg catctgcgaa tgcattgtca actctcttag aacaaaatgt taatttcaga
     3121 aaaatactgt tatcaaaagg aatacacctg aatgttttgg agttaatgca gaagcatata
     3181 cattctcctg aagtggctga aagtggctgt aaaatgctaa atcatctttt tgaaggaagc
     3241 aacacttccc tggatataat ggcagcagtg gtccccaaaa tactaacagt tatgaaacgt
     3301 catgagacat cattaccagt gcagctggag gcgcttcgag ctattttaca ttttatagtg
     3361 cctggcatgc cagaagaatc cagggaggat acagaatttc atcataagct aaatatggtt
     3421 aaaaaacagt gtttcaagaa tgatattcac aaactggtcc tagcagcttt gaacaggttc
     3481 attggaaatc ctgggattca gaaatgtgga ttaaaagtaa tttcttctat tgtacatttt
     3541 cctgatgcat tagagatgtt atccctggaa ggtgctatgg attcagtgct tcacacactg
     3601 cagatgtatc cagatgacca agaaattcag tgtctgggtt taagtcttat aggatacttg
     3661 attacaaaga agaatgtgtt cataggaact ggacatctgc tggcaaaaat tctggtttcc
     3721 agcttatacc gatttaagga tgttgctgaa atacagacta aaggatttca gacaatctta
     3781 gcaatcctca aattgtcagc atctttttct aagctgctgg tgcatcattc atttgactta
     3841 gtaatattcc atcaaatgtc ttccaatatc atggaacaaa aggatcaaca gtttctaaac
     3901 ctctgttgca agtgttttgc aaaagtagct atggatgatt acttaaaaaa tgtgatgcta
     3961 gagagagcgt gtgatcagaa taacagcatc atggttgaat gcttgcttct attgggagca
     4021 gatgccaatc aagcaaagga gggatcttct ttaatttgtc aggtatgtga gaaagagagc
     4081 agtcccaaat tggtggaact cttactgaat agtggatctc gtgaacaaga tgtacgaaaa
     4141 gcgttgacga taagcattgg gaaaggtgac agccagatca tcagcttgct cttaaggagg
     4201 ctggccctgg atgtggccaa caatagcatt tgccttggag gattttgtat aggaaaagtt
     4261 gaaccttctt ggcttggtcc tttatttcca gataagactt ctaatttaag gaaacaaaca
     4321 aatatagcat ctacactagc aagaatggtg atcagatatc agatgaaaag tgctgtggaa
     4381 gaaggaacag cctcaggcag cgatggaaat ttttctgaag atgtgctgtc taaatttgat
     4441 gaatggacct ttattcctga ctcttctatg gacagtgtgt ttgctcaaag tgatgacctg
     4501 gatagtgaag gaagtgaagg ctcatttctt gtgaaaaaga aatctaattc aattagtgta
     4561 ggagaatttt accgagatgc cgtattacag cgttgctcac caaatttgca aagacattcc
     4621 aattccttgg ggcccatttt tgatcatgaa gatttactga agcgaaaaag aaaaatatta
     4681 tcttcagatg attcactcag gtcatcaaaa cttcaatccc atatgaggca ttcagacagc
     4741 atttcttctc tggcttctga gagagaatat attacatcac tagacctttc agcaaatgaa
     4801 ctaagagata ttgatgccct aagccagaaa tgctgtataa gtgttcattt ggagcatctt
     4861 gaaaagctgg agcttcacca gaatgcactc acgagctttc cacaacagct atgtgaaact
     4921 ctgaagagtt tgacacattt ggacttgcac agtaataaat ttacatcatt tccttcttat
     4981 ttgttgaaaa tgagttgtat tgctaatctt gatgtctctc gaaatgacat tggaccctca
     5041 gtggttttag atcctacagt gaaatgtcca actctgaaac agtttaacct gtcatataac
     5101 cagctgtctt ttgtacctga gaacctcact gatgtggtag agaaactgga gcagctcatt
     5161 ttagaaggaa ataaaatatc agggatatgc tcccccttga gactgaagga actgaagatt
     5221 ttaaacctta gtaagaacca catttcatcc ctatcagaga actttcttga ggcttgtcct
     5281 aaagtggaga gtttcagtgc cagaatgaat tttcttgctg ctatgccttt cttgcctcct
     5341 tctatgacaa tcctaaaatt atctcagaac aaattttcct gtattccaga agcaatttta
     5401 aatcttccac acttgcggtc tttagatatg agcagcaatg atattcagta cctaccaggt
     5461 cccgcacact ggaaatcttt gaacttaagg gaactcttat ttagccataa tcagatcagc
     5521 atcttggact tgagtgaaaa agcatattta tggtctagag tagagaaact gcatctttct
     5581 cacaataaac tgaaagagat tcctcctgag attggctgtc ttgaaaatct gacatctctg
     5641 gatgtcagtt acaacttgga actaagatcc tttcccaatg aaatggggaa attaagcaaa
     5701 atatgggatc ttcctttgga tgaactgcat cttaactttg attttaaaca tataggatgt
     5761 aaagccaaag acatcataag gtttcttcaa cagcgattaa aaaaggctgt gccttataac
     5821 cgaatgaaac ttatgattgt gggaaatact gggagtggta aaaccacctt attgcagcaa
     5881 ttaatgaaaa ccaagaaatc agatcttgga atgcaaagtg ccacagttgg catagatgtg
     5941 aaagactggc ctatccaaat aagagacaaa agaaagagag atctcgtcct aaatgtgtgg
     6001 gattttgcag gtcgtgagga attctatagt actcatcccc attttatgac gcagcgagca
     6061 ttgtaccttg ctgtctatga cctcagcaag ggacaggctg aagttgatgc catgaagcct
     6121 tggctcttca atataaaggc tcgcgcttct tcttcccctg tgattctcgt tggcacacat
     6181 ttggatgttt ctgatgagaa gcaacgcaaa gcctgcatga gtaaaatcac caaggaactc
     6241 ctgaataagc gagggttccc tgccatacga gattaccact ttgtgaatgc caccgaggaa
     6301 tctgatgctt tggcaaaact tcggaaaacc atcataaacg agagccttaa tttcaagatc
     6361 cgagatcagc ttgttgttgg acagctgatt ccagactgct atgtagaact tgaaaaaatc
     6421 attttatcgg agcgtaaaaa tgtgccaatt gaatttcccg taattgaccg gaaacgatta
     6481 ttacaactag tgagagaaaa tcagctgcag ttagatgaaa atgagcttcc tcacgcagtt
     6541 cactttctaa atgaatcagg agtccttctt cattttcaag acccagcact gcagttaagt
     6601 gacttgtact ttgtggaacc caagtggctt tgtaaaatca tggcacagat tttgacagtg
     6661 aaagtggaag gttgtccaaa acaccctaag ggcattattt cgcgtagaga tgtggaaaaa
     6721 tttctttcaa aaaaaaggaa atttccaaag aactacatgt cacagtattt taagctccta
     6781 gaaaaattcc agattgcttt gccaatagga gaagaatatt tgctggttcc aagcagtttg
     6841 tctgaccaca ggcctgtgat agagcttccc cattgtgaga actctgaaat tatcatccga
     6901 ctatatgaaa tgccttattt tccaatggga ttttggtcaa gattaatcaa tcgattactt
     6961 gagatttcac cttacatgct ttcagggaga gaacgagcac ttcgcccaaa cagaatgtat
     7021 tggcgacaag gcatttactt aaattggtct cctgaagctt attgtctggt aggatctgaa
     7081 gtcttagaca atcatccaga gagtttctta aaaattacag ttccttcttg tagaaaaggc
     7141 tgtattcttt tgggccaagt tgtggaccac attgattctc tcatggaaga atggtttcct
     7201 gggttgctgg agattgatat ttgtggtgaa ggagaaactc tgttgaagaa atgggcatta
     7261 tatagtttta atgatggtga agaacatcaa aaaatcttac ttgatgactt gatgaagaaa
     7321 gcagaggaag gagatctctt agtaaatcca gatcaaccaa ggctcaccat tccaatatct
     7381 cagattgccc ctgacttgat tttggctgac ctgcctagaa atattatgtt gaataatgat
     7441 gagttggaat ttgaacaagc tccagagttt ctcctaggtg atggcagttt tggatcagtt
     7501 taccgagcag cctatgaagg agaagaagtg gctgtgaaga tttttaataa acatacatca
     7561 ctcaggctgt taagacaaga gcttgtggtg ctttgccacc tccaccaccc cagtttgata
     7621 tctttgctgg cagctgggat tcgtccccgg atgttggtga tggagttagc ctccaagggt
     7681 tccttggatc gcctgcttca gcaggacaaa gccagcctca ctagaaccct acagcacagg
     7741 attgcactcc acgtagctga tggtttgaga tacctccact cagccatgat tatataccga
     7801 gacctgaaac cccacaatgt gctgcttttc acactgtatc ccaatgctgc catcattgca
     7861 aagattgctg actacggcat tgctcagtac tgctgtagaa tggggataaa aacatcagag
     7921 ggcacaccag ggtttcgtgc acctgaagtt gccagaggaa atgtcattta taaccaacag
     7981 gctgatgttt attcatttgg tttactactc tatgacattt tgacaactgg aggtagaata
     8041 gtagagggtt tgaagtttcc aaatgagttt gatgaattag aaatacaagg aaaattacct
     8101 gatccagtta aagaatatgg ttgtgcccca tggcctatgg ttgagaaatt aattaaacag
     8161 tgtttgaaag aaaatcctca agaaaggcct acttctgccc aggtctttga cattttgaat
     8221 tcagctgaat tagtctgtct gacgagacgc attttattac ctaaaaacgt aattgttgaa
     8281 tgcatggttg ctacacatca caacagcagg aatgcaagca tttggctggg ctgtgggcac
     8341 accgacagag gacagctctc atttcttgac ttaaatactg aaggatacac ttctgaggaa
     8401 gttgctgata gtagaatatt gtgcttagcc ttggtgcatc ttcctgttga aaaggaaagc
     8461 tggattgtgt ctgggacaca gtctggtact ctcctggtca tcaataccga agatgggaaa
     8521 aagagacata ccctagaaaa gatgactgat tctgtcactt gtttgtattg caattccttt
     8581 tccaagcaaa gcaaacaaaa aaattttctt ttggttggaa ccgctgatgg caagttagca
     8641 atttttgaag ataagactgt taagcttaaa ggagctgctc ctttgaagat actaaatata
     8701 ggaaatgtca gtactccatt gatgtgtttg agtgaatcca caaattcaac ggaaagaaat
     8761 gtaatgtggg gaggatgtgg cacaaagatt ttctcctttt ctaatgattt caccattcag
     8821 aaactcattg agacaagaac aagccaactg ttttcttatg cagctttcag tgattccaac
     8881 atcataacag tggtggtaga cactgctctc tatattgcta agcaaaatag ccctgttgtg
     8941 gaagtgtggg ataagaaaac tgaaaaactc tgtggactaa tagactgcgt gcacttttta
     9001 agggaggtaa tggtaaaaga aaacaaggaa tcaaaacaca aaatgtctta ttctgggaga
     9061 gtgaaaaccc tctgccttca gaagaacact gctctttgga taggaactgg aggaggccat
     9121 attttactcc tggatctttc aactcgtcga cttatacgtg taatttacaa cttttgtaat
     9181 tcggtcagag tcatgatgac agcacagcta ggaagcctta aaaatgtcat gctggtattg
     9241 ggctacaacc ggaaaaatac tgaaggtaca caaaagcaga aagagataca atcttgcttg
     9301 accgtttggg acatcaatct tccacatgaa gtgcaaaatt tagaaaaaca cattgaagtg
     9361 agaaaagaat tagctgaaaa aatgagacga acatctgttg agtaaaaggg cgaattcgac
     9421 ccagctttct tgtacaaagt ggttgatatc ggtaccagtc gactctagag gatcccgggt
     9481 ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc
     9541 ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct
     9601 ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct
     9661 gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca
     9721 ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg
     9781 gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt
     9841 tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc
     9901 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt
     9961 taaaataact ataccagcag gaggacgtcc agacacagca taggctacct ggccatgccc
    10021 aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt gggtccactc
    10081 agtagatgcc tgttgaattg ggtacgcggc cagcttggct gtggaatgtg tgtcagttag
    10141 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    10201 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    10261 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
    10321 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    10381 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    10441 gcctaggctt ttgcaaaaag ctcctcgagg aactgaaaaa ccagaaagtt aattccctat
    10501 agtgagtcgt attaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
    10561 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    10621 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    10681 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    10741 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    10801 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    10861 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    10921 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    10981 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    11041 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    11101 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    11161 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    11221 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    11281 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    11341 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    11401 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    11461 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    11521 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    11581 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    11641 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    11701 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    11761 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    11821 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    11881 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    11941 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    12001 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    12061 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    12121 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    12181 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    12241 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    12301 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    12361 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    12421 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    12481 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    12541 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    12601 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    12661 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    12721 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    12781 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    12841 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    12901 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    12961 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    13021 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
    13081 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caattt
//
