LOCUS       Exported               10171 bp ds-DNA     circular SYN 08-JUN-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 14, 2020 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10171
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             905..1621
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1:  905 ..  907 / #05fd14
                        2:  908 ..  910 / #05fd14 / 1a
                        3:  911 .. 1621 / #05fd14"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     protein_bind    1643..1667
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     1682..1707
                     /label=Insert.FOR
                     /note="color: black; sequence: atgatggagatccagatggacgaggg"
     primer_bind     complement(5661..5692)
                     /label=Insert.REV
                     /note="color: black; sequence: 
                     tcactcgggggtgtaggacacctgccacacga"
     protein_bind    complement(5706..5730)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     polyA_signal    5768..5992
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      6038..6466
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        6480..6809
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      6660..6795
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             6876..7670
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    7844..7965
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(8014..8030)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    8038..8054
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(8062..8092)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 8062 .. 8068 / #ffffff / -10
                        2: 8069 .. 8086 / #ffffff
                        3: 8087 .. 8092 / #ffffff / -35"
     protein_bind    8107..8128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(8416..9004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(9175..10035)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  9175 ..  9966 / #ccffcc
                        2:  9967 .. 10035 / #ccffcc / signal sequence
                     Cleavage site after base 9966"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10036..10140)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctaga
      901 caccatggcc agcaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      961 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgctac
     1021 atacggaaag cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     1081 aacacttgtc actactttct cttatggtgt tcaatgcttt tcccgttatc cggatcatat
     1141 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat
     1201 atctttcaaa gatgacggga actacaagac gcgtgctgaa gtcaagtttg aaggtgatac
     1261 ccttgttaat cgtatcgagt taaaaggtat tgattttaaa gaagatggaa acattctcgg
     1321 acacaaactc gagtacaact ataactcaca caatgtatac atcacggcag acaaacaaaa
     1381 gaatggaatc aaagctaact tcaaaattcg ccacaacatt gaagatggat ccgttcaact
     1441 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     1501 ccattacctg tcgacacaat ctgccctttc gaaagatccc aacgaaaagc gtgaccacat
     1561 ggtccttctt gagtttgtaa ctgctgctgg gattacacat ggcatggatg agctctacaa
     1621 aagcggttgg cctccggatc aaacaagttt gtacaaaaaa gcaggctccg aattcgccct
     1681 tatgatggag atccagatgg acgagggcgg cggcgtggtg gtgtaccagg acgactactg
     1741 ctccggctcg gtgatgtcgg agcgggtgtc gggcctggcg ggctccatct accgcgagtt
     1801 cgagcgcctc atccactgct acgacgagga ggtggtcaag gagctcatgc cgctggtggt
     1861 gaacgtgctg gagaacctag actcggtgct cagcgagaac caggagcacg aggtggagct
     1921 ggagctgctg cgcgaggaca acgagcagct gctcacccag tacgagcgtg agaaggcgct
     1981 gcgcaggcag gcggaggaga aattcattga gtttgaagat gctctggaac aagagaagaa
     2041 agagctgcaa atccaggtgg agcactacga gttccagacg cgccagctgg agctgaaggc
     2101 caagaactat gccgatcaga tttcccggtt ggaggagcgg gagtcggaga tgaagaagga
     2161 gtacaatgcc ctgcaccagc ggcacacaga gatgatacag acctacgtgg agcacattga
     2221 gaggtccaag atgcagcagg tcggaggaaa cagccagacc gagagcagcc tgccggggcg
     2281 gaggaaggag cgccccacct ccctgaacgt gttccccctg gctgacggca cggtacgtgc
     2341 acagatcggg ggcaagctcg tgcctgcggg ggaccactgg cacctgagtg acctcggcca
     2401 gctgcagtcc agctccagct accagtgtcc acaggatgaa atgtccgagt caggccagtc
     2461 ctcggcggcc gccacaccca gcaccacagg caccaagtcc aacacgccca catcctccgt
     2521 gccctcggcc gccgtcacac ccctcaacga gagcctgcag cccctggggg actatggcgt
     2581 gggctccaag aacagcaagc gtgcccggga gaagcgcgac agccgcaaca tggaagtaca
     2641 ggtcacccag gagatgcgca acgtcagtat aggcatgggc agcagtgacg agtggtctga
     2701 tgttcaagac attattgact ccacgccaga gctggacatg tgtccagaga cccgcctgga
     2761 ccgcacagga agcagcccaa cccagggcat cgtgaacaaa gctttcggca tcaacaccga
     2821 ctccctgtac catgagctgt cgacggcagg gtctgaggtc atcggggatg tggacgaagg
     2881 ggccgacctc ctaggggagt tctcagtgcg cgatgatttc tttggaatgg gcaaagaagt
     2941 ggggaatctg ctactggaaa actcacagct tctggaaacc aaaaacgcct tgaatgtggt
     3001 gaagaatgac ctgattgcca aggtcgacca gctgtccggg gagcaggagg tgctgagggg
     3061 cgagttggag gctgctaagc aggccaaagt caagctggaa aaccgtatca aggagctgga
     3121 agaggaactg aaaagagtga agtccgaggc catcatcgcc cgccgtgaac ccaaagaaga
     3181 ggcggaggat gtaagcagct atctctgtac agaatcggac aaaatcccca tggcccagcg
     3241 ccgccgcttc acgcgggtgg agatggcccg tgtgctcatg gagcggaacc agtacaagga
     3301 gcggctgatg gagctgcagg aggctgtgcg gtggactgag atgatcagag cgtcccgaga
     3361 gcacccatcc gtccaggaga agaagaagtc gaccatctgg cagttcttca gccgcctctt
     3421 cagctcttcc tccagccccc ctccggccaa gcgcccctat ccctcggtga acatccacta
     3481 caagtcaccc accactgccg gcttcagcca gcgccgcaac catgccatgt gcccgatctc
     3541 ggcaggcagc cggcccctgg aattcttccc tgacgacgac tgcacgtcct ccgcccgtcg
     3601 agagcagaag cgcgagcagt accgccaggt gcgtgagcac gtgcgtaacg acgacggccg
     3661 tctgcaggcc tgcggctgga gcctgcccgc caagtacaag cagctgagtc ccaacggggg
     3721 ccaggaggac acgcggatga agaacgtgcc ggtgccggtg tactgccgcc ctctggtgga
     3781 gaaggacccc accatgaagc tgtggtgtgc cgcgggcgtc aacctgagcg ggtggaggcc
     3841 caatgaggac gacgctggga atggagtcaa gccagcgcca ggccgcgatc ccctgacctg
     3901 cgaccgcgaa ggagacggcg agcccaagag cgcccacacg tctcccgaga agaagaaggc
     3961 caaggagctc cctgaaatgg acgccacctc cagccgggtg tggatcctga ccagcaccct
     4021 gaccaccagc aaggtggtga tcatcgacgc caaccagccg ggcacggtgg tggaccagtt
     4081 caccgtctgc aacgcgcacg tgctgtgcat ctccagcatc cccgcggcca gcgacagcga
     4141 ctaccctccc ggggagatgt tcctggacag cgacgtgaac ccagaggacc cgggcgcaga
     4201 tggcgtgctg gccggtatca ccctggtggg ctgtgccacc cgctgcaacg tgccgcggag
     4261 caactgctcc tcccgagggg acaccccagt gctagacaag gggcaggggg aggtggccac
     4321 catcgccaac gggaaggtca acccgtccca gtccacagag gaggccacag aggccacgga
     4381 ggtgccagac cctgggccca gcgagccaga gacagccaca ttgcggcccg ggcctctcac
     4441 agagcacgtc ttcactgacc cagccccgac cccgtcctct ggcccccagc ctggcagcga
     4501 gaacgggcca gagcctgaca gcagcagcac acggccagag ccagagccca gcggggaccc
     4561 cacgggagca ggcagcagtg ctgcacccac catgtggctg ggagcccaga acggctggct
     4621 ctatgtgcac tcggctgtgg ccaactggaa gaagtgcctg cactccatca agctgaagga
     4681 ttctgtgctg agcctggtgc atgtcaaagg ccgtgtgctg gtggctctgg cggacgggac
     4741 cctggccatc ttccaccgtg gtgaagatgg ccagtgggat ctgagcaact atcacctaat
     4801 ggacctgggc cacccgcacc actccatccg ctgcatggct gttgtgtacg accgcgtgtg
     4861 gtgtggctac aagaacaagg tgcacgtcat ccagcccaag accatgcaga tagagaagtc
     4921 atttgacgcc cacccgcggc gggagagcca ggtgcggcag ctggcgtgga tcggcgatgg
     4981 cgtatgggtg tccatccgcc tggactccac cctgaggctc taccatgcac acacgcacca
     5041 gcatctacag gacgtggaca ttgagcccta cgtcagcaag atgctaggca ctggcaagct
     5101 gggtttctcc ttcgtacgca tcacggccct gcttgtcgcg ggcagccggc tctgggtggg
     5161 caccggcaac ggagtggtca tctccatccc cctgacagag actgtggtcc tgcaccgagg
     5221 ccagctcctg gggctccgag ccaataagac atcccccacc tctggggagg gcgcccgtcc
     5281 cgggggcatc atccacgtgt atggcgatga cagcagtgac agggcggcca gcagcttcat
     5341 cccctactgc tccatggccc aggcccagct atgcttccat gggcaccgcg atgccgtgaa
     5401 gttctttgtc tcggtgccag ggaacgtgct ggccaccctg aatgggagtg tgctggacag
     5461 cccagccgag ggccctgggc cagctgcccc tgcctcggag gtcgagggcc agaagctgcg
     5521 gaacgtgctg gtgctgagcg gcggggaggg ctacatcgac ttccgcattg gagacggaga
     5581 ggacgacgag acggaggagg gcgcagggga catgagccag gtgaagcccg tgctgtccaa
     5641 ggcagagcgc agtcacatca tcgtgtggca ggtgtcctac acccccgagt gaaagggcga
     5701 attcgaccca gctttcttgt acaaagtggt gataattaat taagataaac ccgctgatca
     5761 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5821 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5881 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5941 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     6001 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6061 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6121 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6181 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6241 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6301 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6361 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6421 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     6481 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6541 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6601 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     6661 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6721 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     6781 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     6841 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     6901 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     6961 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     7021 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     7081 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     7141 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     7201 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     7261 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     7321 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     7381 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     7441 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     7501 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     7561 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     7621 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     7681 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     7741 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     7801 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     7861 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7921 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     7981 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8041 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8101 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8161 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8221 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8281 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8341 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8401 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8461 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8521 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8581 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8641 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8701 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8761 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8821 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8881 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8941 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9001 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9061 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9121 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9181 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9241 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9301 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9361 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9421 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9481 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9541 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9601 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9661 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9721 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9781 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9841 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9901 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9961 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10021 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10081 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10141 cacatttccc cgaaaagtgc cacctgacgt c
//
