LOCUS       Exported                5266 bp ds-DNA     circular SYN 18-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Kenny
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Dec 16, 2020 from SnapGene Viewer 5.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     93..130
                     /label=tal1_c_fwd
     misc_feature    99..107
                     /label=KOZAC
     CDS             105..1079
                     /codon_start=1
                     /label=Tal1-cds
                     /translation="MMEKLKSEQFPLSPSAEGCASPPRGDGDARGKQEGTTAETGEHRL
                     PEELNGVAKETAHHATELKKEVAVIELSRRGGSADIKGRELKAELSHKVQTTELCRPPI
                     PLPLPPRDPLSDTRMVQLSPPAFPLPARAMLYSNMTTPLATINSGFAGDAEQYGMYPSN
                     RVKRRPAPYEVEINDGSQPKIVRRIFTNSRERWRQQNVNGAFAELRKLIPTHPPDKKLS
                     KNEILRLAMKYINFLAKLLNDQDDMVGGEAPARANRDSRDATLVRDDLLQEMLSPNSSC
                     GSLLDGDASPESFTEDQDSSVESRPSARGLHHSSLPLDGNAQR"
     misc_feature    395
                     /label=silent mutation (c->t)
     misc_feature    419
                     /label=silent mutation (c->a)
     primer_bind     complement(1061..1085)
                     /label=tal1_c_rev
     primer_bind     complement(1562..1582)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1562..1580)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1601..1617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1601..1617)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1614..1636)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1625..1641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1649..1679)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1694..1715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1832..1849)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2003..2591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2083..2102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2762..3622)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3385..3404
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3623..3727)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3753..4208)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3890..3911)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4102..4121
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4282..5266
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atccatcgat tcgaattcgc caccatgatg gaaaaactga
      121 aatccgagca atttccgctg agcccgagtg cggagggctg cgcttctccc ccacgcgggg
      181 atggagacgc gcgcgggaaa caggaaggca cgacggcgga gacaggagag catcgcctcc
      241 ccgaggagct caatggtgtt gccaaggaaa cggctcacca cgccaccgag ctaaaaaagg
      301 aggtggcggt gatcgagctg tccaggagag gagggagcgc ggatataaaa ggcagggagc
      361 tcaaggcaga actcagccat aaggtgcaga ccactgagct gtgcagaccc ccgattccat
      421 tgccactgcc tcccagagac ccgctgagcg acactcgaat ggtgcagttg agtccgcccg
      481 ctttccctct cccggcacga gcgatgctct acagcaacat gacgacgccg ctcgccacta
      541 ttaacagtgg ttttgctgga gatgcggaac agtatgggat gtatcctagc aatcgagtca
      601 agcgcagacc tgcaccttat gaggttgaaa tcaacgatgg ttcgcagccc aaaattgtgc
      661 gacggatttt cacgaacagt cgcgagcgct ggcgacagca gaatgtgaac ggtgccttcg
      721 cagagctgcg caagctcatc ccaacacacc ctcctgacaa gaaactcagc aagaacgaga
      781 tcctgcgtct ggccatgaag tacatcaact ttctggctaa gctcctcaac gaccaggacg
      841 acatggtcgg cggtgaagct cccgcccggg ccaatcggga cagcagagat gcaactctag
      901 tgcgggacga cctccttcag gagatgctaa gccccaactc cagctgcggg agtctgctgg
      961 atggggatgc cagcccagag agctttactg aggaccagga ctcatcagtg gagtccaggc
     1021 cctctgcaag aggactacac cattccagcc taccgctgga cggaaatgcc cagcggtgac
     1081 tcgagcctct agtaattaag tctcagccac cgttaactga acatgtcaaa acctgtggag
     1141 actgttgaga tttgatgttc tgaaaagata aagcctataa ataaaatgtt gcccaaattt
     1201 cctgcctgat gtttttcttt gtctttgcta catggctttg ctgctcggat cggctcactc
     1261 tgtgtatgcc acgttcactt tgtactctcc ttctcacggt aggtttatta tttttagatg
     1321 tgcagttagt ttctgtgaaa taacacacca cacactgata ttgtctgtgc attgacttgg
     1381 tgagtgcaca ttgtttttga tcttgacata tttatatttg attgatcagg tgaactgtgt
     1441 gaatctaaag tgctccatac agatgttctg cattgaaaat attctcattt tattagtgga
     1501 agtgagtgta tgccagcggc cgcggcgcca atgcattggg cccggtaccc agcttttgtt
     1561 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     1621 gaaattgtta tccgctcaca attccacaca acatacgagc cgggagcata aagtgtaaag
     1681 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     1741 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     1801 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1861 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     1921 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1981 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2041 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2101 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2161 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2221 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2281 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2341 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2401 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     2461 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2521 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2581 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2641 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2701 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2761 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2821 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2881 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     2941 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3001 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3061 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3121 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3181 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3241 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3301 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3361 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3421 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3481 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3541 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3601 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3661 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     3721 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     3781 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3841 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     3901 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     3961 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     4021 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     4081 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4141 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4201 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     4261 ctcttcgcta ttacgccagt cgaccatagc caattcaata tggcgtatat ggactcatgc
     4321 caattcaata tggtggatct ggacctgtgc caattcaata tggcgtatat ggactcgtgc
     4381 caattcaata tggtggatct ggaccccagc caattcaata tggcggactt ggcaccatgc
     4441 caattcaata tggcggactt ggcactgtgc caactgggga ggggtctact tggcacggtg
     4501 ccaagtttga ggaggggtct tggccctgtg ccaagtccgc catattgaat tggcatggtg
     4561 ccaataatgg cggccatatt ggctatatgc caggatcaat atataggcaa tatccaatat
     4621 ggccctatgc caatatggct attggccagg ttcaatacta tgtattggcc ctatgccata
     4681 tagtattcca tatatgggtt ttcctattga cgtagatagc ccctcccaat gggcggtccc
     4741 atataccata tatggggctt cctaataccg cccatagcca ctcccccatt gacgtcaatg
     4801 gtctctatat atggtctttc ctattgacgt catatgggcg gtcctattga cgtatatggc
     4861 gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt
     4921 caataggacc acccaccatt gacgtcaatg ggatggctca ttgcccattc atatccgttc
     4981 tcacgccccc tattgacgtc aatgacggta aatggcccac ttggcagtac atcaatatct
     5041 attaatagta acttggcaag tacattacta ttggaaggac gccagggtac attggcagta
     5101 ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc tgccaagtac atccccattg
     5161 acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag
     5221 gcgtgcctaa tgggaggtct atataagcaa tgctcgttta gggaac
//
