LOCUS       Exported               10942 bp ds-DNA     circular SYN 17-DEC-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 17, 2020 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10942
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     protein_bind    1966..1984
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2002..2020
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2038..2056
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2074..2092
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2110..2128
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2146..2164
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    2182..2200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     regulatory      2346..2355
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             2352..6770
                     /codon_start=1
                     /label=Flag-GluN2A
                     /note="color: #993366"
                     /translation="MGRLGYWTLLVLPALLVWRDPAQNAAAEKGPPALNIAVLLGHSHD
                     VTERELRNLDYKDDDDKWGPEQATGLPLDVNVVALLMNRTDPKSLITHVCDLMSGARIH
                     GLVFGDDTDQEAVAQMLDFISSQTFIPILGIHGGASMIMADKDPTSTFFQFGASIQQQA
                     TVMLKIMQDYDWHVFSLVTTIFPGYRDFISFIKTTVDNSFVGWDMQNVITLDTSFEDAK
                     TQVQLKKIHSSVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFP
                     SGLISVSYDDWDYSLEARVRDGLGILTTAASSMLEKFSYIPEAKASCYGQAEKPETPLH
                     TLHQFMVNVTWDGKDLSFTEEGYQVHPRLVVIVLNKDREWEKVGKWENQTLSLRHAVWP
                     RYKSFSDCEPDDNHLSIVTLEEAPFVIVEDIDPLTETCVRNTVPCRKFVKINNSTNEGM
                     NVKKCCKGFCIDILKKLSRTVKFTYDLYLVTNGKHGKKVNNVWNGMIGEVVYQRAVMAV
                     GSLTINEERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVMMFVMLLIV
                     SAIAVFVFEYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTS
                     KIMVSVWAFFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRFGTVP
                     NGSTERNIRNNYPYMHQYMTRFNQRGVEDALVSLKTGKLDAFIYDAAVLNYKAGRDEGC
                     KLVTIGSGYIFATTGYGIALQKGSPWKRQIDLALLQFVGDGEMEELETLWLTGICHNEK
                     NEVMSSQLDIDNMAGVFYMLAAAMALSLITFIWEHLFYWKLRFCFTGVCSDRPGLLFSI
                     SRGIYSCIHGVHIEEKKKSPDFNLTGSQSNMLKLLRSAKNISNMSNMNSSRMDSPKRAT
                     DFIQRGSLIVDMVSDKGNLIYSDNRSFQGKDSIFGDNMNELQTFVANRHKDNLSNYVFQ
                     GQHPLTLNESNPNTVEVAVSTESKGNSRPRQLWKKSMESLRQDSLNQNPVSQRDEKTAE
                     NRTHSLKSPRYLPEEVAHSDISETSSRATCHREPDNNKNHKTKDNFKRSMASKYPKDCS
                     DVDRTYMKTKASSPRDKIYTIDGEKEPSFHLDPPQFVENITLPENVGFPDTYQDHNENF
                     RKGDSTLPMNRNPLHNEDGLPNNDQYKLYAKHFTLKDKGSPHSEGSDRYRQNSTHCRSC
                     LSNLPTYSGHFTMRSPFKCDACLRMGNLYDIDEDQMLQETGNPATREEVYQQDWSQNNA
                     LQFQKNKLRINRQHSYDNILDKPREIDLSRPSRSISLKDRERLLEGNLYGSLFSVPSSK
                     LLGNKSSLFPQGLEDSKRSKSLLPDHASDNPFLHTYGDDQRLVIGRCPSDPYKHSLPSQ
                     AVNDSYLRSSLRSTASYCSRDSRGHSDVYISEHVMPYAANKNTMYSTPRVLNSCSNRRV
                     YKKMPSIESDV"
     CDS             2514..2537
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     misc_feature    6795..7383
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(7266..7277)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 7265"
                     /translation="IEGR"
     LTR             7463..7696
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    7768..7889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      7929..8064
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        complement(8085..8103)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(8113..8129)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      8271..8726
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        8752..8856
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             8857..9717
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8857 .. 8925 / #ccffcc / signal sequence
                        2: 8926 .. 9717 / #ccffcc
                     Cleavage site after base 8925"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9888..10476
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    10764..10785
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        10800..10830
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 10800 .. 10805 / #ffffff / -35
                        2: 10806 .. 10823 / #ffffff
                        3: 10824 .. 10830 / #ffffff / -10"
     protein_bind    10838..10854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     10862..10878
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        10899..10917
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatacg cgtatcacga gactagcctc gagagttggc tttactccct atcagtgata
     1981 gagaacgtat gaagagttta ctccctatca gtgatagaga acgtatgcag actttactcc
     2041 ctatcagtga tagagaacgt ataaggagtt tactccctat cagtgataga gaacgtatga
     2101 ccagtttact ccctatcagt gatagagaac gtatctacag tttactccct atcagtgata
     2161 gagaacgtat atccagttta ctccctatca gtgatagaga acgtataagc tttaggcgtg
     2221 tacggtgggc gcctataaaa gcagagctcg tttagtgaac cgtcagatcg cctggagcaa
     2281 ttccacaaca cttttgtctt ataccaactt tccgtaccac ttcctaccct cgtaaagaat
     2341 tcgccgccac catgggcaga ttgggctact ggaccttgct ggtattgccg gcccttctgg
     2401 tctggcgcga tccggcgcag aacgcggcgg cggagaaggg tcctccagcg ctgaacattg
     2461 cggtgctgct gggtcacagc cacgacgtga cagaacgcga acttcgaaat ctggactaca
     2521 aggacgacga tgacaagtgg ggcccagagc aggcaaccgg cttgcccctg gatgtgaacg
     2581 tggtggcgtt attgatgaac cgcactgacc ctaagagcct catcacgcat gtgtgcgacc
     2641 tcatgtccgg ggcgcgcatc cacggcttgg tgtttggaga tgacacggac caggaggctg
     2701 tggcccagat gctggatttt atctcctcac agacttttat ccccatcttg ggcattcatg
     2761 ggggtgcatc tatgatcatg gctgacaagg atccgacatc cacgttcttc cagtttggag
     2821 cctccatcca gcagcaagcc acagttatgc tgaagatcat gcaggactac gactggcacg
     2881 tcttctccct ggtcaccacc atcttccctg gctaccgaga cttcatcagc tttatcaaga
     2941 caacagtgga caacagcttt gtgggctggg atatgcagaa cgtgatcaca ctggacacct
     3001 ccttcgagga cgccaagacg caggtccagc tgaagaagat ccattcttct gtcatcctgc
     3061 tctactgctc caaagacgag gctgtcctca tcctgagcga ggctcgctcc ctcggcctca
     3121 ctggctatga tttcttctgg attgtcccca gtttggtgtc tgggaacaca gagctcatcc
     3181 ccaaagagtt tccatcaggt ctcatttcag tctcttatga cgactgggac tacagcctgg
     3241 aggcaagagt gagagacggt cttgggatct taaccactgc cgcatcctcc atgttggaga
     3301 agttctccta cattcctgag gccaaggcca gctgctatgg gcaggcagag aagccagaga
     3361 ccccgctaca caccctgcac caattcatgg tcaatgtgac ttgggatggc aaggacttgt
     3421 ccttcactga ggaaggttac caggtgcacc ccaggcttgt ggtgatcgtg ctgaacaagg
     3481 accgggagtg ggaaaaggtg ggcaagtggg agaaccagac gctgagcctg aggcacgctg
     3541 tgtggccaag gtacaagtcc ttttctgact gcgagccaga tgacaaccac ctcagcattg
     3601 tcaccttgga ggaagccccc ttcgtcatcg tagaggacat agaccccctg actgagacct
     3661 gtgtgaggaa cacggtgccc tgtcggaagt tcgtcaagat caacaattca accaacgaag
     3721 ggatgaatgt gaagaaatgc tgcaaggggt tctgcatcga catcctcaag aagctgtcca
     3781 gaactgtgaa gttcacctat gacctctacc tggtgaccaa tgggaagcat gggaaaaagg
     3841 ttaacaatgt gtggaatgga atgataggtg aagtggtcta tcaacgagca gtcatggctg
     3901 tgggctcact caccatcaat gaggagcgtt cggaagtggt ggacttctcg gtgcccttcg
     3961 tggagacagg aatcagcgtc atggtctcca ggagtaatgg cactgtctcc ccttctgctt
     4021 tcctcgaacc cttcagtgcc tccgtctggg tgatgatgtt tgtgatgctg ctcatcgtct
     4081 cagccattgc tgtcttcgtt tttgaatact tcagtcctgt tggatacaac agaaacttag
     4141 ccaaagggaa agctccccac gggccttctt ttactattgg aaaagctata tggctcctct
     4201 ggggcctggt cttcaacaat tctgtgcctg tccagaatcc taaaggcaca accagcaaga
     4261 tcatggtgtc agtgtgggcc ttctttgctg tcatcttcct ggccagttac acagccaacc
     4321 tggctgcctt catgatccag gaggagtttg tggaccaagt gactggcctc agtgacaaga
     4381 agttccagag acctcatgac tattctccac ctttccgatt tgggacggta cccaatggaa
     4441 gtacagagag gaatattcgt aacaactacc cgtatatgca ccagtacatg accagattca
     4501 accagagggg agtggaggat gccttggtca gcttgaaaac cgggaagttg gacgctttca
     4561 tctatgacgc agccgtcttg aactacaagg ccgggaggga tgaaggctgt aaactggtga
     4621 ccattgggag cgggtacatc tttgctacca caggctatgg aattgcgctg cagaagggct
     4681 caccctggaa gaggcagatt gacctcgctc tgctccagtt tgttggtgat ggtgagatgg
     4741 aggagctgga gacactgtgg cttacgggca tctgccacaa cgagaagaat gaggtgatga
     4801 gtagccagct ggacatcgat aacatggcgg gcgtgttcta catgctggct gcagccatgg
     4861 ccctcagcct catcaccttc atctgggagc acctcttcta ctggaagctg cgcttctgct
     4921 tcacaggcgt gtgctctgac cggcccgggc tgctcttctc catcagcagg ggcatctata
     4981 gttgcatcca tggggtacac attgaagaaa agaagaaatc tccagatttc aatctgactg
     5041 gatcacagag caacatgcta aagcttcttc ggtcagctaa aaacatctcc aatatgtcca
     5101 acatgaactc ctcaagaatg gactcaccta aaagagctac tgacttcatt caaagagggt
     5161 cacttattgt ggacatggtt tcagacaagg gaaatttgat atattcagac aacagatcct
     5221 ttcaagggaa ggacagtata tttggagaca acatgaatga actccaaaca tttgtggcca
     5281 acaggcacaa ggataatctc agtaactatg tgtttcaagg acagcatcct ctcactctca
     5341 atgagtccaa ccctaacaca gtagaggtgg ctgtcagcac tgaatccaaa gggaactccc
     5401 gaccccggca gctttggaag aaatccatgg agtctctacg ccaggattct ctaaaccaga
     5461 acccagtctc ccagagggat gagaagactg cagagaatcg gacccactcg ctaaagagtc
     5521 ctaggtatct tccagaagag gtagcccact ctgacatttc agaaacctca agccgggcca
     5581 catgccacag ggagccagat aacaataaga accacaagac caaggataac ttcaaacggt
     5641 caatggcctc taagtatccc aaggactgta gcgatgttga ccgcacctac atgaaaacca
     5701 aagcaagttc tcccagggat aagatctata ccattgatgg tgagaaggag cccagcttcc
     5761 acttagatcc tcctcagttt gttgagaata taaccctgcc tgagaatgtg ggcttcccag
     5821 atacctacca agatcacaat gagaacttcc gcaaggggga ctccacactg cccatgaaca
     5881 ggaacccatt acataatgaa gacgggcttc ccaacaatga ccaatataaa ctctatgcca
     5941 agcactttac cttgaaagac aagggttccc cacacagtga gggcagtgat cgataccggc
     6001 agaactccac acattgcaga agctgccttt cgaatctgcc cacctactca ggccacttta
     6061 ccatgaggtc tcctttcaag tgtgatgcct gtctgcggat ggggaatctc tatgacattg
     6121 atgaagacca gatgcttcag gagacaggta acccagctac tcgggaggag gtctaccagc
     6181 aggactggtc acagaacaac gccctccagt tccagaagaa caagctaagg attaaccgac
     6241 agcactccta tgataacatt ctggacaaac ccagagagat agaccttagc aggccctccc
     6301 ggagcataag cctcaaggac agggaacggc tactggaggg caacttgtat gggagcctgt
     6361 tcagtgtccc ctcaagcaaa ctcttgggga acaaaagctc ccttttcccc caaggtctgg
     6421 aggacagcaa gaggagcaag tctctcttgc cagaccacgc ctccgataat cctttcctcc
     6481 acacgtatgg ggatgaccaa cgcttagtta tcgggagatg tccctcggac ccttacaaac
     6541 actcattgcc atcacaggcg gtaaatgaca gctatcttcg gtcatccttg aggtcaacag
     6601 catcatattg ctccagggac agtcggggcc acagtgatgt gtatatttca gagcatgtta
     6661 tgccttatgc tgcaaataag aataccatgt actctacccc cagggtttta aattcctgca
     6721 gcaatagacg agtgtacaag aaaatgccta gtatcgaatc tgatgtttaa ctcgaccggt
     6781 tagtaatgat cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6841 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6901 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6961 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     7021 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     7081 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     7141 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     7201 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7261 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7321 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7381 cgcctggcga tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7441 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     7501 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7561 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7621 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7681 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     7741 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     7801 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7861 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     7921 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7981 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8041 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     8101 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     8161 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     8221 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     8281 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     8341 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     8401 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     8461 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     8521 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     8581 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     8641 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     8701 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     8761 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     8821 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8881 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     8941 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     9001 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     9061 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     9121 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9181 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9241 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9301 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9361 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9421 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9481 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9541 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9601 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9661 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9721 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9781 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9841 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9901 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9961 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    10021 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10081 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10141 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10201 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10261 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10321 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10381 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10441 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10501 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    10561 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    10621 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    10681 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
    10741 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    10801 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    10861 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
    10921 caaaagctgg agctgcaagc tt
//
