LOCUS       Exported                6844 bp ds-DNA     circular SYN 17-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 17, 2020 from SnapGene 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    11..82
                     /label=CMV promoter
     misc_feature    626..2131
                     /label=B2 [*]
     misc_feature    881..883
                     /label=E61C
     misc_feature    2202..2786
                     /label=IRES
     CDS             2793..3506
                     /label=EGFP
     rep_origin      3770..4210
                     /direction=RIGHT
                     /label=F1 ori
     promoter        4277..4305
                     /label=Amp prom
     promoter        4509..4705
                     /label=SV40 early promoter
     misc_feature    4556..4633
                     /label=SV40 ORI
     CDS             4743..5534
                     /label=Kan/neoR
     rep_origin      6120..6748
                     /direction=RIGHT
                     /label=ColE1 origin
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagcg ccaccatggc ccggtgctcc aactctatgg cgctgctgtt
      661 cagctttggc ctcctttggc tgtgttcagg ggttttgggt actgacacag aggagcggct
      721 ggtggagcat ctcttggatc cttcccgcta taacaagctg atccgtccag ctactaatgg
      781 ctctgagctg gtgactgtac agctcatggt atcattggca cagctcatca gtgtgcacga
      841 gcgggagcag atcatgacca ccaacgtctg gctgacccag tgctgggaag attatcgcct
      901 cacatggaag cctgaggatt tcgacaatat gaagaaagtc cgacttcctt ctaagcacat
      961 ctggctccca gatgtggttc tatacaacaa tgctgacggc atgtacgaag tctccttcta
     1021 ttccaatgct gtggtctcct atgatggcag catcttttgg ctaccgcctg ccatctacaa
     1081 gagcgcatgc aagattgagg tgaagcactt cccatttgac cagcagaact gcaccatgaa
     1141 gttccgctcc tggacctacg accgcactga gattgacctg gtgctcaaaa gcgatgtggc
     1201 cagcctggac gacttcacac ctagtgggga gtgggacatc atcgcactgc caggccgacg
     1261 caacgagaac ccagacgact ccacctacgt ggacatcacc tacgacttca tcattcgtcg
     1321 caaaccgctc ttctacacca tcaacctcat catcccctgc gtactcatca cctcgctggc
     1381 catcctggtc ttctacctgc cctcagactg tggtgaaaag atgacacttt gtatttctgt
     1441 gctgctggcg ctcacggtgt tcctgctgct catctccaag attgtgcctc ccacctccct
     1501 cgacgtaccg ctggtgggaa agtacctcat gttcaccatg gtgctagtca ccttctccat
     1561 cgtcactagc gtgtgtgtgc tcaatgtgca ccaccgttcg cctaccacgc acaccatggc
     1621 gccctgggtc aaggtggtct tcctggagaa gctgcccacc ctcctcttcc tgcagcagcc
     1681 acgccaccgc tgtgcacgtc aacgcctgcg cttgcgaagg cgccagcggg aacgtgaggg
     1741 ggcaggcaca ctattcttcc gcgaaggtcc tgcagctgac ccatgtacct gctttgtcaa
     1801 tcctgcatca atgcagggct tggctggggc tttccaggct gagcccgctg cagccggtct
     1861 ggggcgctcg atgggcccat gcagctgtgg cctccgggaa gctgtggacg gtgtacgctt
     1921 cattgcggac catatgcgaa gtgaagatga tgaccagagt gtgagggagg attggaaata
     1981 cgttgccatg gtgatcgacc gcctgttcct ctggatcttt gtctttgtct gtgtctttgg
     2041 gaccattggc atgttcctgc agcctctctt ccagaactac actgccacga ccttccttca
     2101 ctctgaccac tcagctccca gctccaagtg aatcccgcgg ccatggcggc cgggagcatg
     2161 cgacgtcggg cccggtaccg aattcatagc tagcatcgat cgcccctctc cctccccccc
     2221 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     2281 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     2341 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     2401 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     2461 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     2521 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     2581 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     2641 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     2701 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     2761 aaaaacacga tgataatatg gccacaacca tggtgagcaa gggcgaggag ctgttcaccg
     2821 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     2881 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     2941 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     3001 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     3061 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     3121 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     3181 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     3241 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     3301 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     3361 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     3421 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     3481 ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctgtcgacca taatcagcca
     3541 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     3601 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     3661 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3721 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
     3781 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     3841 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     3901 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     3961 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     4021 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     4081 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     4141 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     4201 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4261 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4321 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     4381 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4441 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4501 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     4561 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4621 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4681 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     4741 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     4801 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     4861 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     4921 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     4981 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     5041 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     5101 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     5161 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     5221 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     5281 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     5341 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     5401 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     5461 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     5521 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     5581 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     5641 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     5701 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     5761 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     5821 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     5881 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     5941 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
     6001 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6061 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6121 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6181 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6241 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     6301 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6361 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6421 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6481 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6541 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6601 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6661 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6721 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6781 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
     6841 gcat
//
