LOCUS       Exported                8407 bp ds-DNA     circular SYN 17-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 17, 2020 from SnapGene 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        209..863
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     CDS             901..3924
                     /label=Grid2wt [*]
     misc_feature    2929..2931
                     /label=I677C
     primer_bind     complement(3960..3977)
                     /label=SP6
     terminator      4000..4227
                     /label=bGH PA
     misc_feature    4896..4973
                     /label=SV40 ORI
     CDS             5115..5906
                     /label=Kan/neoR
     primer_bind     complement(6246..6266)
                     /label=M13-rev
     misc_binding    complement(6272..6294)
                     /label=LacO
     promoter        complement(6299..6328)
                     /label=lac
     rep_origin      complement(6634..7262)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(7414..8073)
                     /label=AmpR
     promoter        complement(8313..8341)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 atggaagttt tccccttgct cttgtttctg tccttctgtt ggtctcgaac ctgggacctg
      961 gcgaccgctg attctatcat ccacatcgga gcaatttttg atgaatctgc taaaaaagat
     1021 gatgaagtat tccgcacagc agttggtgac ctcaaccaga atgaggaaat cttacagact
     1081 gagaaaatca cattttctgt gacgtttgtt gatggcaaca acccttttca agctgttcaa
     1141 gaagcatgtg aacttatgaa ccagggcatc ttggccttgg tcagctccat tggttgcaca
     1201 tcagctgggt ccctccagtc tttggcagat gccatgcata tcccccacct cttcattcag
     1261 cgctcaacag ctgggacccc aagaagtggc tgtggcctca cccggagcaa cagaaatgat
     1321 gactatactc tttcagttcg tccacctgtc tacttgaatg aagtcatcct aagagtagtc
     1381 acagagtatg catggcagaa atttattatc ttctatgaca gtgaatatga tatccgtgga
     1441 atccaggaat ttttggacaa agtatcccag cagggaatgg atgttgccct tcaaaaggtg
     1501 gaaaacaaca tcaacaaaat gatcaccaca ctctttgaca ccatgaggat agaggagttg
     1561 aatcgctatc gagacactct tagaagagcc atccttgtca tgaatcctgc cacagccaaa
     1621 tccttcataa gtgaggtggt ggagacgaat ctggttgctt ttgactgtca ctggatcatc
     1681 attaatgagg aaataaatga tgtggatgtt caggaacttg tcagaaggtc cattggaagg
     1741 ttaacaataa ttcggcagac atttccagtc ccccagaata taagtcagcg ctgtttccgt
     1801 ggcaaccatc gaatttcttc atcactgtgt gatccgaagg atccctttgc acagaatatg
     1861 gagatttcta acctttacat ctatgacacg gtgcttctgc ttgcgaatgc cttccataag
     1921 aagctggagg accgtaagtg gcacagcatg gcaagcttgt cctgtatcag gaaaaactcc
     1981 aagccctggc aaggagggcg gtccatgctg gagaccatca agaagggtgg agttaatgga
     2041 ttgactggag atctagaatt tggagaaaat ggaggtaacc ccaatgtcca ctttgaaatc
     2101 cttggaacca actatggaga agaacttggc aggggtgtcc gtaaacttgg gtgctggaat
     2161 cctgtcacag gtctgaatgg atcactgaca gacaaaaaac tggagaacaa catgcggggc
     2221 gtggttctac gagtggtgac tgtattggag gagccttttg taatggtctc tgaaaatgtc
     2281 ttgggaaaac cgaagaaata ccagggcttc tccattgatg ttttggatgc cctatctaac
     2341 tacctgggtt ttaattatga aatttatgtt gcaccggacc acaaatatgg aagcccacaa
     2401 gaggatggga catggaatgg actggtagga gaacttgttt ttaagagagc tgacattggg
     2461 atttcagctt taaccatcac tccagaccgg gagaatgttg tagattttac aacacgctac
     2521 atggactact cagtgggagt ccttcttcga agggcagaaa aaacagtgga tatgtttgcc
     2581 tgccttgcac catttgatct atctctgtgg gcctgcattg ctggcacagt gcttcttgtg
     2641 ggactcttgg tctacctctt aaactggctt aatccccctc gattgcaaat gggatcgatg
     2701 acatctacta cactctacaa ctccatgtgg tttgtgtatg ggtcctttgt acagcaaggt
     2761 ggggaagtcc catacacaac tctggcaacc cgaatgatga tgggggcttg gtggttattt
     2821 gctctgattg tcatctcatc ttatacagcc aaccttgccg ctttcctcac tatcacccgc
     2881 attgagagct ccatccagtc tcttcaggat ctgtccaagc aaacagattg cccttatggc
     2941 acagtcttgg actcggcagt atatcagcat gtccgcatga aggggctgaa tcctttcgag
     3001 agagacagca tgtattccca gatgtggagg atgatcaacc gaagcaatgg gtcagaaaac
     3061 aatgttttgg agtcccaagc aggcattcaa aaggtaaaat atggaaacta tgcttttgta
     3121 tgggatgcag ctgtgttgga atatgtggct attaacgacc ccgactgttc cttctacact
     3181 gttgggaaca ctgttgctga ccgggggtat ggcattgcac tgcagcatgg cagcccctac
     3241 cgtgatgtct tttcacaaag gatcctggag ctgcagcaga gtggcgacat ggacattctc
     3301 aagcacaaat ggtggcctaa gaacggccag tgtgacctgt actcctcagt ggatgcaaag
     3361 cagaagggag gagccttgga catcaagagc ctggcaggcg tgttctgcat cctggccgcg
     3421 ggaattgtgc tctcctgcct catagctgtc ctcgagacgt ggtggagccg gaggaagggc
     3481 tcccgggtcc catcgaaaga ggatgacaag gaaattgacc tggagcacct ccatagacgt
     3541 gtaaatagct tgtgcacaga tgacgacagc ccccataaac agttttccac ctcgtcaatt
     3601 gacttgaccc ctctggacat tgacactttg ccaacacgcc aagccctgga gcagatcagt
     3661 gatttcagaa acactcacat caccaccacc accttcatcc cagagcagat ccagactctt
     3721 agccgcacac tctcagcaaa agctgcctct ggcttcgctt tcggcagtgt gcctgagcac
     3781 cgaactggcc cttttaggca cagggctcct aatgggggct ttttcaggag tcctatcaaa
     3841 acaatgtcat ctattcctta tcagcctact cccactctgg ggctcaatct gggcaatgac
     3901 cccgaccgag gcacgtccat atgagcggcc gctcgagcat gcatctagag ggccctattc
     3961 tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     4021 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     4081 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     4141 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     4201 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     4261 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4321 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4381 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag
     4441 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4501 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4561 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4621 cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt
     4681 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4741 cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4801 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4861 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4921 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4981 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     5041 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     5101 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     5161 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     5221 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     5281 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     5341 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     5401 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     5461 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     5521 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     5581 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     5641 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     5701 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     5761 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     5821 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     5881 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     5941 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     6001 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     6061 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     6121 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6181 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     6241 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6301 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6361 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6421 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6481 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6541 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6601 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6661 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6721 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6781 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6841 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6901 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6961 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7021 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7081 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7141 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7201 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7261 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7321 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7381 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7441 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7501 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7561 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7621 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7681 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7741 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7801 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7861 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7921 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7981 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8041 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8101 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8161 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8221 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8281 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8341 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8401 tgacgtc
//
