LOCUS       pcDNA3.1\NanoLuc        6196 bp    DNA     circular      7-DEC-2020
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-13639502|
COMMENT     VNTDBDATE|-13639502|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pcDNA3.1 NanoLuc-PM|
COMMENT     VNTAUTHORNAME|Andras Toth|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pcDNA3.1 NanoLuc-PM" 0 6196 
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COMMENT         "NcoI" "NdeI" "NheI" "NotI" "PauI" "PstI" "SalI" "SmaI" "XhoI" "SacI" 
COMMENT         "EcoNI" "Bsp1407I" "PvuI" "BoxI" "XagI" "Bst1107I" "MluI" "AflII" 
COMMENT         "ClaI" "XbaI" "SpeI" "VspI" "EcoRV" "SphI" "SexAI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT       (CObList #0=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 918 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 33 0 4698 0 5941 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1546 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BglII" "agatct" 2 0 2 13 0 1552 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 912 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 611 0 2730 0 3465 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 485 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 896 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 1748 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "PauI" "gcgcgc" 2 0 1 3430 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 3086 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 4010 0 6195 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 2846 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1754 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 819 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "EcoNI" "cctnnnnnagg" 6 0 1 1087 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "Bsp1407I" "tgtaca" 2 0 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 5644 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "BoxI" "gacnnnngtc" 6 0 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "XagI" "cctnnnnnagg" 6 0 1 1087 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "Bst1107I" "gtatac" 4 0 1 4005 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 229 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 909 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 1760 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 250 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "VspI" "attaat" 3 0 6 258 0 863 0 2494 0 4155 
COMMENT             0 4214 0 5446 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "SphI" "gcatgc" 6 0 4 1997 0 2571 0 2643 0 
COMMENT             3438 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "SexAI" "accwggt" 2 0 1 2590 0 0 "")) 
COMMENT       (CObList 
COMMENT        #32=(CFSignal (CObList) "T7 for" 21 0 0 849 882 0 (CStringList) 
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COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #34=(CFSignal (CObList) "IL-6 secretory signal" 4 0 0 930 1016 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #35=(CFSignal (CObList) "NanoLuc" 4 0 0 1017 1526 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #36=(CFSignal (CObList) "PDGFR transmembrane domain" 4 0 0 1593 1745 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #37=(CFSignal (CObList) "linker" 4 0 0 1527 1592 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #38=(CFSignal (CObList) "NanoLuc-PM" 4 0 0 930 1745 0 (CStringList) 
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COMMENT                          "DNA 'pcDNA3.1 NanoLuc-PM'" 1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 12/07/20 07:17du." 1) 
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COMMENT                          "Last Modified: 12/07/20 07:17du." 1) 
COMMENT                     #46=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6196 bp" 1) 
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COMMENT                                 "IL-6 secretory signal") 
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COMMENT                                 "NanoLuc-PM") 
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COMMENT                                        "Start: 1784  End: 1810" 1))) "BGH rev"))) 
COMMENT                          "Misc. Feature (2 total)"))) "Feature Map") 
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COMMENT                          "CTTAAG" "GAATTC") 
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COMMENT                          "ACCGGT" "TGGCCA") 
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COMMENT                          5 "GTGCAC" "CACGTG") 
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COMMENT                          "GGATCC" "CCTAGG") 
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COMMENT                          3 3 "GTATAC" "CATATG") 
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COMMENT                          "CCTNNNNNAGG" "GGANNNNNTCC") 
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COMMENT                           1 5 "CCATGG" "GGTACC") 
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COMMENT                           5 "GCTAGC" "CGATCG") 
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COMMENT                           1 "CTGCAG" "GACGTC") 
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COMMENT                           2 "CGATCG" "GCTAGC") 
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COMMENT                           1 6 "ACCWGGT" "TGGWCCA") 
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FEATURES             Location/Qualifiers
     misc_feature    849..882
                     /vntifkey="21"
                     /label=T7\for
     misc_feature    1784..1810
                     /vntifkey="21"
                     /label=BGH\rev
     CDS             930..1016
                     /vntifkey="4"
                     /label=IL-6\secretory\signal
     CDS             1017..1526
                     /vntifkey="4"
                     /label=NanoLuc
     CDS             1593..1745
                     /vntifkey="4"
                     /label=PDGFR\transmembrane\domain
     CDS             1527..1592
                     /vntifkey="4"
                     /label=linker
     CDS             930..1745
                     /vntifkey="4"
                     /label=NanoLuc-PM
BASE COUNT     1411 a      1621 c      1606 g      1558 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttaccg gtcgccacca tgaactcctt ctccacaagc gccttcggtc 
      961 cagttgcctt ctccctgggc ctgctcctgg tgttgcctgc tgccttccct gccccagtct 
     1021 tcacactcga agatttcgtt ggggactggc gacagacagc cggctacaac ctggaccaag 
     1081 tccttgaaca gggaggtgtg tccagtttgt ttcagaatct cggggtgtcc gtaactccga 
     1141 tccaaaggat tgtcctgagc ggtgaaaatg ggctgaagat cgacatccat gtcatcatcc 
     1201 cgtatgaagg tctgagcggc gaccaaatgg gccagatcga aaaaattttt aaggtggtgt 
     1261 accctgtgga tgatcatcac tttaaggtga tcctgcacta tggcacactg gtaatcgacg 
     1321 gggttacgcc gaacatgatc gactatttcg gacggccgta tgaaggcatc gccgtgttcg 
     1381 acggcaaaaa gatcactgta acagggaccc tgtggaacgg caacaaaatt atcgacgagc 
     1441 gcctgatcaa ccccgacggc tccctgctgt tccgagtaac catcaacgga gtgaccggct 
     1501 ggcggctgtg cgaacgcatt ctggcgggag gcggatcagg tggcggatcc agatctggtg 
     1561 gcggttctgg tggcggtagt ggtggcggta gtaatgctgt gggccaggac acgcaggagg 
     1621 tcatcgtggt gccacactcc ttgcccttta aggtggtggt gatctcagcc atcctggccc 
     1681 tggtggtgct caccatcatc tcccttatca tcctcatcat gctttggcag aagaagccac 
     1741 gttaggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg 
     1801 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 
     1861 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 
     1921 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 
     1981 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc 
     2041 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt 
     2101 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 
     2161 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 
     2221 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 
     2281 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 
     2341 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 
     2401 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 
     2461 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag 
     2521 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 
     2581 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 
     2641 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 
     2701 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 
     2761 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 
     2821 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag 
     2881 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 
     2941 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 
     3001 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 
     3061 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga 
     3121 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 
     3181 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 
     3241 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 
     3301 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 
     3361 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 
     3421 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 
     3481 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 
     3541 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 
     3601 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 
     3661 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 
     3721 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 
     3781 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 
     3841 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta 
     3901 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 
     3961 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag 
     4021 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     4081 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 
     4141 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     4201 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     4261 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     4321 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     4381 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     4441 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     4501 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     4561 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     4621 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     4681 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     4741 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     4801 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     4861 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 
     4921 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     4981 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     5041 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     5101 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     5161 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
     5221 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
     5281 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
     5341 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
     5401 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
     5461 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 
     5521 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 
     5581 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 
     5641 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 
     5701 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 
     5761 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 
     5821 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 
     5881 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 
     5941 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 
     6001 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 
     6061 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
     6121 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
     6181 agtgccacct gacgtc 
//
