LOCUS       pEYFP-N1\GLuc-PM        5507 bp    DNA     circular      7-DEC-2020
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13639480|
COMMENT     VNTDBDATE|-13639480|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEYFP-N1 GLuc-PM-Venus|
COMMENT     VNTAUTHORNAME|Andras Toth|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 1199 0 1435 0 0 "") 
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COMMENT        #27=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
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COMMENT                           "GACGCNNNNNNNNNNN" "CTGCGNNNNNNNNNNN") 
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COMMENT                           1 "GGTACC" "CCATGG") 
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COMMENT                           1 5 "CCATGG" "GGTACC") 
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COMMENT                           3 "CCCGGG" "GGGCCC") 
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COMMENT       (CSeqView 60 1 (CObjectList) (CObList) 0 (CObList)) (CObList) 808585741 
COMMENT       (CStringList) 1818632289 1630368623 
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COMMENT        #600=(CSampFrag "pEYFP-N1 GlucM23-OM-mVenus" 1904 3964 "NcoI" 361 "NcoI" 
COMMENT              (LOGPEN 0 0 0) 1.6322e+069 9.79946e-313) 
COMMENT        #601=(CSampFrag "pEYFP-N1 GlucM23-OM-mVenus" 1809 1452 "NcoI" 3261 
COMMENT              "NcoI" (LOGPEN 0 0 0) 6.96589e-086 4.57863e-310) 
COMMENT        #602=(CSampFrag "pEYFP-N1 GlucM23-OM-mVenus" 828 624 "NcoI" 1452 "NcoI" 
COMMENT              (LOGPEN 0 0 0) 1.85119e+016 1.61877e-312) 
COMMENT        #603=(CSampFrag "pEYFP-N1 GlucM23-OM-mVenus" 703 3261 "NcoI" 3964 "NcoI" 
COMMENT              (LOGPEN 0 0 0) 2.10342e-316 2.5779e-312) 
COMMENT        #604=(CSampFrag "pEYFP-N1 GlucM23-OM-mVenus" 263 361 "NcoI" 624 "NcoI" 
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FEATURES             Location/Qualifiers
     CDS             1453..2172
                     /vntifkey="4"
                     /label=Venus
     misc_feature    2071..2073
                     /vntifkey="21"
                     /label=monomeric\mutation
     CDS             1246..1395
                     /vntifkey="4"
                     /label=PDGFR-transmembrane\domain
     CDS             625..1179
                     /vntifkey="4"
                     /label=GLucM23
     CDS             625..2172
                     /vntifkey="4"
                     /label=GLuc-PM-Venus
BASE COUNT     1305 a      1500 c      1489 g      1213 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgaggc caccatggga gtcaaagttc tgtttgccct gatctgcatc 
      661 gctgtggccg aggccaagcc caccgagaac aacgaagact tcaacatcgt ggccgtggcc 
      721 agcaacttcg cgaccacgga tctcgatgct gaccgcggga agttgcccgg cgagaagctg 
      781 ccgctggagg tgctcaaaga gcttgaagcc aatgcccgga aagctggctg caccaggggc 
      841 tgtctgatct gcctgtccca catcaagtgc acgcccaaga tgaagaagtt catcccagga 
      901 cgctgccaca cctacgaagg cgacaaagag tccgcacagg gcggcatagg cgaggcgatc 
      961 gatgacattc ctgagattcc tgggttcaag gacttggagc ccattgagca gttcatcgca 
     1021 caggtcgatc tgtgtgtgga ctgcacaact ggctgcctca aagggcttgc caacgtgcag 
     1081 tgttctgacc tgctcaagaa gtggctgccg caacgctgtg cgacctttgc cagcaagatc 
     1141 cagggccagg tggacaagat caagggggcc ggtgatgacg gaggcggatc aggtggcgga 
     1201 tccagatctg gtggcggttc tggtggcggt agtggtggcg gtagtaatgc tgtgggccag 
     1261 gacacgcagg aggtcatcgt ggtgccacac tccttgccct ttaaggtggt ggtgatctca 
     1321 gccatcctgg ccctggtggt gctcaccatc atctccctta tcatcctcat catgctttgg 
     1381 cagaagaagc cacgtaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca 
     1441 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
     1501 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
     1561 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg 
     1621 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc 
     1681 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
     1741 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 
     1801 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     1861 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac 
     1921 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc 
     1981 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     2041 cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc 
     2101 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     2161 ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg 
     2221 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg 
     2281 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 
     2341 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 
     2401 tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta 
     2461 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 
     2521 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca 
     2581 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 
     2641 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta 
     2701 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg 
     2761 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg 
     2821 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca 
     2881 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 
     2941 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 
     3001 aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa 
     3061 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 
     3121 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 
     3181 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 
     3241 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 
     3301 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 
     3361 tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga 
     3421 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa 
     3481 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt 
     3541 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg 
     3601 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa 
     3661 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac 
     3721 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt 
     3781 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact 
     3841 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg 
     3901 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg 
     3961 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc 
     4021 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt 
     4081 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc 
     4141 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg 
     4201 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 
     4261 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 
     4321 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc 
     4381 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa 
     4441 gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg 
     4501 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt 
     4561 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg 
     4621 gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac 
     4681 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 
     4741 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 
     4801 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 
     4861 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 
     4921 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 
     4981 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 
     5041 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 
     5101 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 
     5161 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 
     5221 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 
     5281 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 
     5341 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 
     5401 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 
     5461 ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat 
//
