LOCUS       pEYFP-N1\NanoLuc        5549 bp    DNA     circular      7-DEC-2020
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13639477|
COMMENT     VNTDBDATE|-13639477|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEYFP-N1 NanoLuc-PM-Venus|
COMMENT     VNTAUTHORNAME|Andras Toth|
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COMMENT              (LOGPEN 0 0 0) 2.10549e-316 2.0078e-296) 
COMMENT        #318=(CSampFrag "pEYFP-N1 Nluc-OM-mVenus" 1809 1494 "NcoI" 3303 "NcoI" 
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FEATURES             Location/Qualifiers
     CDS             1495..2214
                     /vntifkey="4"
                     /label=Venus
     misc_feature    2113..2115
                     /vntifkey="21"
                     /label=monomeric\mutation
     CDS             712..1221
                     /vntifkey="4"
                     /label=NanoLuc
     CDS             1288..1437
                     /vntifkey="4"
                     /label=PDGFR\transmembrane\domain
     CDS             625..2214
                     /vntifkey="4"
                     /label=NanoLuc-PM-Venus
     CDS             625..711
                     /vntifkey="4"
                     /label=IL-6\secretory\signal
BASE COUNT     1311 a      1507 c      1482 g      1249 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgaggc caccatgaac tccttctcca caagcgcctt cggtccagtt 
      661 gccttctccc tgggcctgct cctggtgttg cctgctgcct tccctgcccc agtcttcaca 
      721 ctcgaagatt tcgttgggga ctggcgacag acagccggct acaacctgga ccaagtcctt 
      781 gaacagggag gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac tccgatccaa 
      841 aggattgtcc tgagcggtga aaatgggctg aagatcgaca tccatgtcat catcccgtat 
      901 gaaggtctga gcggcgacca aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct 
      961 gtggatgatc atcactttaa ggtgatcctg cactatggca cactggtaat cgacggggtt 
     1021 acgccgaaca tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc 
     1081 aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg 
     1141 atcaaccccg acggctccct gctgttccga gtaaccatca acggagtgac cggctggcgg 
     1201 ctgtgcgaac gcattctggc gggaggcgga tcaggtggcg gatccagatc tggtggcggt 
     1261 tctggtggcg gtagtggtgg cggtagtaat gctgtgggcc aggacacgca ggaggtcatc 
     1321 gtggtgccac actccttgcc ctttaaggtg gtggtgatct cagccatcct ggccctggtg 
     1381 gtgctcacca tcatctccct tatcatcctc atcatgcttt ggcagaagaa gccacgtaag 
     1441 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg 
     1501 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 
     1561 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 
     1621 ctgaccctga agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 
     1681 accaccctgg gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac 
     1741 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 
     1801 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 
     1861 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 
     1921 gagtacaact acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc 
     1981 aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac 
     2041 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 
     2101 agctaccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 
     2161 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc 
     2221 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa 
     2281 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact 
     2341 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 
     2401 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag 
     2461 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc 
     2521 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc 
     2581 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 
     2641 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 
     2701 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg 
     2761 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 
     2821 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 
     2881 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga 
     2941 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 
     3001 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg 
     3061 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 
     3121 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 
     3181 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 
     3241 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     3301 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 
     3361 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa 
     3421 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg 
     3481 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct 
     3541 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac 
     3601 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg 
     3661 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 
     3721 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa 
     3781 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca 
     3841 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt 
     3901 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc 
     3961 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc 
     4021 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg 
     4081 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt 
     4141 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag 
     4201 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa 
     4261 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct 
     4321 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 
     4381 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg 
     4441 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg 
     4501 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata 
     4561 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc 
     4621 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca 
     4681 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 
     4741 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 
     4801 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 
     4861 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 
     4921 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 
     4981 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 
     5041 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 
     5101 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 
     5161 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 
     5221 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 
     5281 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 
     5341 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 
     5401 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 
     5461 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 
     5521 ctgtggataa ccgtattacc gccatgcat 
//
