LOCUS       pVER-lacI               4314 bp ds-DNA     circular     20-DEC-2020
DEFINITION  .
ACCESSION   urn.local...bi-csiuvan
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    101..916
                     /locus_tag="KanR"
                     /note="Geneious type: gene"
                     /standard_name="Kanamycin Kinase"
     misc_feature    complement(988..1824)
                     /locus_tag="p15A Origin of Replication"
                     /note="Geneious type: rep_origin"
                     /standard_name="p15A origin of replication"
     misc_feature    complement(1997..2113)
                     /locus_tag="Native AraC Terminator (2SNP)"
                     /note="Geneious type: terminator"
                     /standard_name="Native araC terminator"
     CDS             complement(2135..3217)
                     /standard_name="lacI"
     misc_feature    complement(3246..3299)
                     /locus_tag="PLacI (up until -1)"
                     /note="Geneious type: promoter"
                     /standard_name="PlacI"
     misc_feature    complement(3304..3364)
                     /locus_tag="L3S2P21 Terminator"
                     /note="Geneious type: terminator"
                     /standard_name="L3S2P21 terminator"
     misc_feature    complement(3365..4084)
                     /locus_tag="YFP (3SNP)"
                     /note="Geneious type: CDS"
                     /standard_name="YFP"
     misc_feature    complement(4091..4102)
                     /locus_tag="B0064 RBS"
                     /note="Geneious type: RBS"
                     /standard_name="RBS"
     misc_feature    complement(4111..4185)
                     /locus_tag="RiboJ Ribozyme-insulator"
                     /note="Geneious type: insulator"
                     /standard_name="riboJ ribozyme insulator"
     protein_bind    complement(4186..4206)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /standard_name="lac operator"
     misc_feature    complement(4213..4242)
                     /locus_tag="PTac Promoter"
                     /note="Geneious type: promoter"
                     /standard_name="Ptac"
     misc_feature    complement(4243..4257)
                     /locus_tag="Pre-PTac Spacer"
                     /standard_name="prePtac spacer"
     misc_feature    complement(4262..4314)
                     /locus_tag="L3S3P21 Terminator"
                     /note="Geneious type: terminator"
                     /standard_name="L3S3P21 Terminator"
ORIGIN      
        1 ttgtgtctca aaatctctga tgtacattgc acaagataaa aatatatcat catgaacaat
       61 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
      121 aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
      181 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
      241 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
      301 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
      361 tatccgtact cctgatgatg catggttact caccactgcg atccccggga aaacagcatt
      421 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
      481 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
      541 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
      601 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc
      661 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
      721 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
      781 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
      841 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
      901 cgatgagttt ttctaaggta actgtcagac caagtttact catatatact ttagattgat
      961 ttttcgttcc actgagcgtc agacccctta ataagatgat cttcttgaga tcgttttggt
     1021 ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct tgcagggcgg tttttcgaag
     1081 gttctctgag ctaccaactc tttgaaccga ggtaactggc ttggaggagc gcagtcacca
     1141 aaacttgtcc tttcagttta gccttaaccg gcgcatgact tcaagactaa ctcctctaaa
     1201 tcaattacca gtggctgctg ccagtggtgc ttttgcatgt ctttccgggt tggactcaag
     1261 acgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc
     1321 cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga gacaaacgcg
     1381 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg
     1441 agccgccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat
     1501 ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggctttg
     1561 ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga aatctccgcc
     1621 ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag
     1681 cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcaccggtg cagccttttt
     1741 tctcctgcca catgaagcac ttcactgaca ccctcatcag tgccaacata gtaagccagt
     1801 atacactccg ctagcgctga ggtctgcctc gtgcagcgag tcagtgagcg aggaagcacc
     1861 tcagataaaa tatttgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
     1921 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
     1981 tccggcgtag aggatctgct catgtttgac agcttatcat cgatgcataa tgtgcctgtc
     2041 aaatggacga agcagggatt ctgcaaaccc tatgctactc cctcgagccg tcaattgtct
     2101 gattcgttac caattatttt ttcctcctgg attatcactg cccgctttcc agtcgggaaa
     2161 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2221 tgggcgccag ggtggttttt cttttcacca gtgagactgg caacagctga ttgcccttca
     2281 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     2341 aatcctgttt gatggtggtt aacggcggga tataacatga gctatcttcg gtatcgtcgt
     2401 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     2461 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     2521 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     2581 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     2641 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     2701 gctccacgcc cagtcgcgta ccgtcctcat gggagaaaat aatactgttg atgggtgtct
     2761 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     2821 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     2881 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     2941 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     3001 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     3061 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     3121 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     3181 catactctgc gacatcgtat aacgttactg gtttcatatt caccaccctg aattgactct
     3241 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gcgcgccgcc
     3301 attggaccaa aacgaaaaaa ggcccccctt tcgggaggcc tcttttctgg aatttggtac
     3361 cgagttactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
     3421 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tggtagctaa
     3481 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat tggggtgttc tgctggtagt
     3541 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga
     3601 tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca
     3661 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
     3721 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
     3781 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
     3841 gcagcttcat gtggtcgggg tagcgggcga agcattgcag gccgtagccg aaggtggtca
     3901 cgagggtggg ccagggcacg ggcagcttgc ctgtggtgca gatgaacttc agggtcagct
     3961 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt
     4021 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
     4081 ccatctagta tttcccctct ttctctagta ttaaacaaaa ttatttgtag aggctgtttc
     4141 gtcctcacgg actcatcaga ccggaaagca catccggtga cagctaattg ttatccgctc
     4201 acaattccac acattatacg agccgatgat taattgtcaa cacagccaac gatcgttaag
     4261 cgggagacca gaaacaaaaa aaggcccccc gttagggagg ccttcaataa ttgg
//
