LOCUS       pTY.1-lacI              5580 bp ds-DNA     circular     20-DEC-2020
DEFINITION  .
ACCESSION   urn.local...ba-csiutay
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    101..916
                     /locus_tag="KanR"
                     /note="Geneious type: gene"
                     /standard_name="Kanamycin Kinase"
     misc_feature    complement(988..1824)
                     /locus_tag="p15A Origin of Replication"
                     /note="Geneious type: rep_origin"
                     /standard_name="p15A origin of replication"
     terminator      complement(1904..1951)
     misc_feature    complement(2045..2161)
                     /locus_tag="Native AraC Terminator (2SNP)"
                     /note="Geneious type: terminator"
                     /standard_name="Native araC terminator"
     misc_feature    complement(2183..3265)
                     /locus_tag="LacI"
                     /note="Geneious type: CDS"
                     /standard_name="lacI"
     misc_feature    complement(3294..3347)
                     /locus_tag="PLacI (up until -1)"
                     /note="Geneious type: promoter"
                     /standard_name="PlacI"
     terminator      3355..3415
     promoter        3422..3450
     protein_bind    3456..3476
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /standard_name="lac operator"
     misc_feature    3477..3551
                     /locus_tag="RiboJ Ribozyme-insulator"
                     /note="Geneious type: insulator"
                     /standard_name="riboJ ribozyme insulator"
     RBS             3563..3570
     gene            3578..4780
                     /standard_name="tetA"
     CDS             4804..5523
                     /standard_name="YFP"
     misc_feature    complement(5528..5580)
                     /locus_tag="L3S3P21 Terminator"
                     /note="Geneious type: terminator"
                     /standard_name="L3S3P21 Terminator"
ORIGIN      
        1 ttgtgtctca aaatctctga tgtacattgc acaagataaa aatatatcat catgaacaat
       61 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
      121 aacgtcttgc tccaggccgc gattaaattc caacatggat gctgatttat atgggtataa
      181 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
      241 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
      301 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
      361 tatccgtact cctgatgatg catggttact caccactgcg atccccggga aaacagcatt
      421 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
      481 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
      541 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
      601 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc
      661 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
      721 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
      781 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
      841 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
      901 cgatgagttt ttctaaggta actgtcagac caagtttact catatatact ttagattgat
      961 ttttcgttcc actgagcgtc agacccctta ataagatgat cttcttgaga tcgttttggt
     1021 ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct tgcagggcgg tttttcgaag
     1081 gttctctgag ctaccaactc tttgaaccga ggtaactggc ttggaggagc gcagtcacca
     1141 aaacttgtcc tttcagttta gccttaaccg gcgcatgact tcaagactaa ctcctctaaa
     1201 tcaattacca gtggctgctg ccagtggtgc ttttgcatgt ctttccgggt tggactcaag
     1261 acgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc
     1321 cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga gacaaacgcg
     1381 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg
     1441 agccgccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat
     1501 ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggctttg
     1561 ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga aatctccgcc
     1621 ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag
     1681 cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcaccggtg cagccttttt
     1741 tctcctgcca catgaagcac ttcactgaca ccctcatcag tgccaacata gtaagccagt
     1801 atacactccg ctagcgctga ggtctgcctc gtgcagcgag tcagtgagcg aggaagcacc
     1861 tcagataaaa tatttgctca tgagcccgaa gtggcgagcc cgacaaaaaa cccctcaaga
     1921 cccgtttaga ggccccaagg ggttatgcta gtcttcccca tcggtgatgt cggcgatata
     1981 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     2041 gatctgctca tgtttgacag cttatcatcg atgcataatg tgcctgtcaa atggacgaag
     2101 cagggattct gcaaacccta tgctactccc tcgagccgtc aattgtctga ttcgttacca
     2161 attatttttt cctcctggat tatcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     2221 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg
     2281 tggtttttct tttcaccagt gagactggca acagctgatt gcccttcacc gcctggccct
     2341 gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga
     2401 tggtggttaa cggcgggata taacatgagc tatcttcggt atcgtcgtat cccactaccg
     2461 agatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca
     2521 tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg
     2581 tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt
     2641 gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta
     2701 atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca
     2761 gtcgcgtacc gtcctcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat
     2821 caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat
     2881 ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg
     2941 ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt
     3001 gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg
     3061 aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg
     3121 gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt
     3181 ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga
     3241 catcgtataa cgttactggt ttcatattca ccaccctgaa ttgactctct tccgggcgct
     3301 atcatgccat accgcgaaag gttttgcgcc attcgatggc gcgccgccat aaatctcggt
     3361 accaaattcc agaaaagagg cctcccgaaa ggggggcctt ttttcgtttt ggtccgagct
     3421 gttgacaatt aatcatcggc tcgtataatg tgtggaattg tgagcggata acaattagct
     3481 gtcaccggat gtgctttccg gtctgatgag tccgtgagga cgaaacagcc tctacaaata
     3541 attttgttta atactagcca tcaaggagag ctgctacatg agtagcagta cgaaaattgc
     3601 gcttgtcatc accctcctgg atgcgatggg gatcggcttg atcatgccgg tactgccaac
     3661 ccttctgcgc gagttcattg caagcgaaga tattgccaac catttcgggg ttctgctcgc
     3721 actgtacgcc ttaatgcagg tcatctttgc tccctggtta ggcaaaatgt cagacagctt
     3781 tggacgccgt cctgttttgc tgttaagcct tatcggagcg agcctggatt accttttatt
     3841 ggccttctcc tcggcactgt ggatgcttta tttgggtcgt ttgctgagtg ggattacagg
     3901 cgcgacgggt gccgtggcgg cgtcggtgat tgctgatacg acgtccgcaa gtcaacgtgt
     3961 gaaattgttc ggctggttag gagcctcctt tggcttgggc ttaatcgctg ggccaattat
     4021 tggcgggttc gccggcgaaa tctcaccaca ttcccctttt ttcatcgcgg cattactcaa
     4081 cattgtcacg ttcctggtgg tgatgttctg gttccgcgaa acgaaaaaca cccgcgataa
     4141 cacggataca gaggtggggg ttgaaacgca atcgaacagt gtgtacatca cgctcttcaa
     4201 gaccatgccc atcctgctca tcatctactt ctccgcacag ttgattgggc aaatcccggc
     4261 cacagtgtgg gttttgttta cggaaaaccg tttcgggtgg aactccatga tggtgggttt
     4321 ctctctggct ggattgggac ttctgcatag tgttttccag gctttcgtcg ctggccgtat
     4381 tgccacaaag tggggagaaa aaaccgctgt attgcttggt tttatcgcag atagctctgc
     4441 gtttgccttc ttggcattta ttagcgaagg ctggctcgtg tttccggtat tgattctgtt
     4501 ggctgggggc ggtatcgcat tacccgcgct gcagggagtt atgtctattc aaaccaaatc
     4561 acaccaacaa ggagcgctgc aaggcttact tgtgtccctg accaacgcaa ccggagtcat
     4621 cgggccactt ctgttcgctg taatttataa ccactcactg ccaatttggg atggatggat
     4681 ctggatcatc ggtcttgcct tctactgcat catcattttg ctgtcaatga cattcatgct
     4741 gacgcctcaa gcccaaggat ctaaacaaga aacgagtgcc taacggcgta aggaggtatt
     4801 tttatggtgt caaagggtga ggaactgttt acggggatcg tcccgattct tgttgaactt
     4861 gacggcgacg taaatggtca caagttttcc gtatcgggcg aaggtgaggg cgatgcgact
     4921 tatgggaaat taacactgaa attcatttgc accaccggaa aactgcccgt tccttggcct
     4981 actctggtaa ccacgttcgg atatggttta cagtgttttg ctcgctaccc ggaccatatg
     5041 aaactgcacg atttcttcaa gtccgccatg ccggagggct acgtgcagga acgtacaatc
     5101 ttcttcaaag acgatggtaa ttacaagacc cgtgctgaag ttaaatttga gggggatact
     5161 ttagtcaatc gtattgaatt gaaggggatt gactttaagg aagacggtaa tatccttggc
     5221 cacaagcttg aatacaacta caatagtcac aatgtgtata ttatggctga taaacagaag
     5281 aatggcatta aggttaactt taagatccgt cacaatatcg aagacggatc tgtccagctt
     5341 gctgaccatt atcagcaaaa tacgccgatc ggtgacggtc cggttttgtt gccggacaac
     5401 cattacctgt cctatcagtc ggcgttaagt aaagatccga atgagaaacg cgaccatatg
     5461 gttttgttgg agtttgtgac ggctgctggc attacgcttg ggatggacga gctgtataaa
     5521 taaaagcggg agaccagaaa caaaaaaagg ccccccgtta gggaggcctt caataattgg
//
