LOCUS       Exported                8936 bp ds-DNA     circular SYN 25-DEC-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    eSpCas9(1.1)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 25, 2020 from SnapGene 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8936
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..241
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    110..131
                     /label=Primer 1 forward
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    250..269
                     /label=gRNA 1
                     /note="color: #00ff00"
     misc_RNA        270..345
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
                     /note="color: #00ccff; direction: RIGHT"
     promoter        436..676
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    545..566
                     /label=Primer 1 forward
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    685..704
                     /label=gRNA 2
                     /note="color: #00ff00"
     misc_RNA        705..780
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the CRISPR/Cas9 system"
                     /note="color: #00ccff; direction: RIGHT"
     enhancer        870..1155
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
                     /note="color: #ffffff"
     misc_feature    complement(937..956)
                     /label=Primer 2 reverse
                     /note="color: #a6acb3; direction: LEFT"
     promoter        1157..1434
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     intron          1435..1663
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
                     /note="color: #------"
     CDS             1684..1749
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2
                     Cleavage site after base 1749"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1756..1776
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             1801..3516
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /note="color: #993366"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             3403..5901
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /note="color: #993366"
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             5902..5949
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    5983..6190
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     repeat_region   6199..6339
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     rep_origin      6414..6869
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        7151..7255
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             7256..8116
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7256 .. 7324 / #ccffcc / signal sequence
                        2: 7325 .. 8116 / #ccffcc
                     Cleavage site after base 7324"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8287..8875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg aacactagtg cacttatccg ttttagagct agaaatagca agttaaaata
      301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag
      361 ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag
      421 acaaatggct ctagagaggg cctatttccc atgattcctt catatttgca tatacgatac
      481 aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa
      541 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
      601 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
      661 atcttgtgga aaggacgaaa caccgaaacc atatggcgct ccaggtttta gagctagaaa
      721 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
      781 ttttttgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt tttagcgcgt
      841 gcgccaattc tgcagacaaa tggggtaccc gttacataac ttacggtaaa tggcccgcct
      901 ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc
      961 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     1021 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     1081 tgtgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     1141 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     1201 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     1261 gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     1321 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     1381 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     1441 gctgcgacgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
     1501 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
     1561 ctgtaattag ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat
     1621 gtttaattac ctggagcacc tgcctgaaat cacttttttt caggttggac cggtgccacc
     1681 atggactata aggaccacga cggagactac aaggatcatg atattgatta caaagacgat
     1741 gacgataaga tggccccaaa gaagaagcgg aaggtcggta tccacggagt cccagcagcc
     1801 gacaagaagt acagcatcgg cctggacatc ggcaccaact ctgtgggctg ggccgtgatc
     1861 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
     1921 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
     1981 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
     2041 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
     2101 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
     2161 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
     2221 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
     2281 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
     2341 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
     2401 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg
     2461 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg
     2521 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
     2581 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
     2641 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
     2701 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
     2761 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
     2821 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
     2881 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
     2941 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag
     3001 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
     3061 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
     3121 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
     3181 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
     3241 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac
     3301 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
     3361 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
     3421 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
     3481 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
     3541 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
     3601 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
     3661 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
     3721 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
     3781 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
     3841 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc
     3901 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
     3961 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
     4021 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
     4081 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
     4141 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
     4201 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
     4261 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc
     4321 gtgcctcaga gctttctggc ggacgactcc atcgacaaca aggtgctgac cagaagcgac
     4381 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
     4441 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
     4501 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
     4561 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
     4621 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
     4681 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
     4741 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
     4801 cctgcgctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
     4861 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
     4921 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaaggcgcct
     4981 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
     5041 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
     5101 acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc
     5161 agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat
     5221 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
     5281 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
     5341 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
     5401 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag
     5461 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
     5521 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
     5581 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
     5641 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
     5701 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
     5761 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag
     5821 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
     5881 ctgtctcagc tgggaggcga caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa
     5941 aagaaaaagt aagaattcct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     6001 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     6061 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     6121 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagag aatagcaggc
     6181 atgctgggga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     6241 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     6301 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc
     6361 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     6421 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     6481 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     6541 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6601 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     6661 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6721 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
     6781 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6841 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     6901 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     6961 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     7021 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     7081 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     7141 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7201 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7261 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7321 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7381 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7441 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     7501 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7561 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7621 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7681 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7741 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7801 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7861 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7921 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg gaagccgcgg
     7981 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8041 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8101 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8161 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8221 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8281 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8341 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8401 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     8461 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8521 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8581 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8641 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8701 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8761 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8821 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8881 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
