LOCUS       Exported               10792 bp ds-DNA     circular SYN 25-DEC-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10792)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 25, 2020 from SnapGene 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10792
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..2848
                     /label=Backbone
                     /note="color: #ffcc99"
     protein_bind    11..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(35..65)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 35 .. 41 / #ffffff / -10
                        2: 42 .. 59 / #ffffff
                        3: 60 .. 65 / #ffffff / -35"
     misc_feature    complement(75..97)
                     /label=Primer 747
                     /note="color: #a6acb3; direction: LEFT"
     protein_bind    80..101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(389..974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(1145..2005)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 1145 .. 1936 / #ccffcc
                        2: 1937 .. 2005 / #ccffcc / signal sequence
                     Cleavage site after base 1936"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2006..2110)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      2137..2592
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     2732..2749
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    2852..3540
                     /label=5' homology arm
                     /note="color: #3366ff"
     misc_feature    complement(3013..3033)
                     /label=Primer 661
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    3444..3465
                     /label=Primer 662
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        3554..4732
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    complement(3666..3686)
                     /label=Primer 764
                     /note="color: #a6acb3; direction: LEFT"
     intron          3785..4723
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     misc_feature    4685..4704
                     /label=Primer 718
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4778..6194
                     /label=STOP cassette
                     /note="color: #ccffcc"
     protein_bind    4778..4811
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    complement(4833..4853)
                     /label=Primer 868
                     /note="color: #a6acb3; direction: LEFT"
     intron          5345..5410
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
                     /note="color: #------"
     CDS             5540..5560
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    5985..6119
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     protein_bind    6161..6194
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    6213..6218
                     /label=Kozak
                     /note="color: #ff6600"
     misc_feature    6219..7943
                     /label=TIR1
                     /note="color: #ff0000"
     misc_feature    complement(6317..6336)
                     /label=Primer 858
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    7831..7854
                     /label=Primer 881
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             7944..7952
                     /codon_start=1
                     /label=Linker sequence
                     /note="Linker sequence"
                     /note="color: #ffff00"
                     /translation="GGS"
     CDS             7953..7979
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     polyA_signal    8099..8220
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     protein_bind    complement(8250..8283)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
                     /note="color: #31849b; direction: LEFT"
     promoter        8305..8804
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    complement(8394..8413)
                     /label=Primer 665
                     /note="color: #a6acb3; direction: LEFT"
     CDS             8844..9443
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9521..9973
                     /label=PGK polyA
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    9889..9910
                     /label=Primer 666
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    complement(10012..10045)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
                     /note="color: #31849b; direction: LEFT"
     misc_feature    10068..10787
                     /label=3' homology arm
                     /note="color: #3366ff"
     misc_feature    complement(10371..10391)
                     /label=Primer 667
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    10707..10727
                     /label=Primer 668
                     /note="color: #a6acb3; direction: RIGHT"
ORIGIN
        1 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
       61 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      121 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      181 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      241 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      301 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      361 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      421 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      481 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      541 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      601 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      661 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      721 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      781 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
      841 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      901 gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      961 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1021 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1081 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1141 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1201 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1261 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1321 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1381 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1441 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1501 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1561 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1621 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1681 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1741 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1801 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1861 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1921 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1981 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2041 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2101 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
     2161 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     2221 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     2281 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     2341 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     2401 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     2461 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     2521 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     2581 cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2641 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2701 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctgat
     2761 ctatacattg aatcaatatt ggcaattagc catattagtc attggttata tagcataaat
     2821 caatattggc tattggccat tgcatacgtt taggacttct ggaatttctt ctgcacataa
     2881 agttctgata gcattagttt aagctggact aaccctgaaa gtagcttgtg gcaagtatca
     2941 aggaatcaat attatactct acaaaatcaa agtttacaga gaagtcatat agtaattttt
     3001 ctgaaattta ctggcacaat gttaatccag cctgactcca actaattaat ggtcacatta
     3061 atttaagtct ttcccttgcc tctgctgcat tagtttctct caaaattgtt aacttacaac
     3121 ttgaagtctg gtattataaa ttgaatgtaa agcattctga aagatactat actgattgca
     3181 ggtttttcag tcaggttcaa gctaatttga ccagtcattg gattaattat ggatctgggg
     3241 ccataaatgc tattttaatt ccactataga gattaaaata agccattctc catttcataa
     3301 tattctattg gactttgact gcaggggcct ccaagtcttg acagtagatt ataatccttc
     3361 agctgcccac tctactggag gaggacaaac tggtcacttt tcagcaaaac ctggctgtgg
     3421 atcagggcag tctggtactt ccaagctcat tagatgccat catgctctca ctgcctcctc
     3481 agcttcaaga ggaatctgga aaaagcagtc ccactggtca ggaaaggaac actagtgcac
     3541 aaacggctcg aggggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     3601 cgagaagttg tggggagggg tcggcaattg aagcggtgcc tagagaaggt ggcgcggggt
     3661 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     3721 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     3781 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     3841 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     3901 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     3961 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     4021 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     4081 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     4141 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     4201 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     4261 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     4321 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     4381 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     4441 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     4501 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     4561 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     4621 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     4681 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggggatcg
     4741 tcgatgtgct gtctcatcat tttggcaaag aattctaata acttcgtata gcatacatta
     4801 tacgaagtta tattaagggt tccggatcct cggggacacc aaatatggcg atctcggcct
     4861 tttcgtttct tggagctggg acatgtttgc catcgatcca tctaccacca gaacggccgt
     4921 tagatctgct gccaccgttg tttccaccga agaaaccacc gttgccgtaa ccaccacgac
     4981 ggttgttgct aaagaagctg ccaccgccac ggccaccgtt gtagccgccg ttgttgttat
     5041 tgtagttgct catgttattt ctggcacttc ttggttttcc tcttaagtga ggaggaacat
     5101 aaccattctc gttgttgtcg ttgatgctta aattttgcac ttgttcgctc agttcagcca
     5161 taatatgaaa tgcttttctt gttgttctta cggaatacca cttgccacct atcaccacaa
     5221 ctaacttttt cccgttcctc catctctttt atattttttt tctcgaggga tctttgtgaa
     5281 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     5341 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     5401 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     5461 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     5521 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     5581 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     5641 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     5701 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
     5761 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
     5821 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
     5881 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     5941 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     6001 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6061 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     6121 gacatggtaa gtaagcttgg gctgcaggtc gagggaccta ataacttcgt atagcataca
     6181 ttatacgaag ttatatgtcg acgccactcg acgccaccat gacctacttc cccgaggaag
     6241 tggtggaaca catcttcagc tttctgcccg cccagaggga cagaaacacc gtgtccctcg
     6301 tgtgcaaagt gtggtacgag atcgagagac tgagcagacg gggcgtgttc gtgggcaact
     6361 gttacgctgt gcgggctggc agagtggccg ccagatttcc taatgtgcgg gccctgaccg
     6421 tgaagggcaa gcctcacttc gccgacttca acctggtgcc ccctgattgg ggcggctacg
     6481 ctggaccttg gatcgaggct gctgccagag gctgtcacgg cctggaagaa ctgagaatga
     6541 agagaatggt ggtgtccgac gagagcctgg aactgctggc cagaagcttc cccagattca
     6601 gagccctggt gctgatctcc tgcgagggct tctctacaga tggcctggcc gccgtggcca
     6661 gccattgcaa gctgctgaga gagctggatc tccaggaaaa cgaggtggaa gatagaggcc
     6721 ccagatggct gagctgcttc cccgacagct gtaccagcct ggtgtccctg aacttcgcct
     6781 gcatcaaggg ggaagtgaac gccggcagcc tggaaagact ggtgtccaga agccccaacc
     6841 tgagaagcct gagactgaac agatccgtgt ccgtggacac cctggccaag atcctgctga
     6901 gaacccctaa cctggaagat ctgggcaccg gcaacctgac cgacgacttc cagaccgaga
     6961 gctacttcaa gctgaccagc gccctggaaa agtgcaagat gctgcggagc ctgagcggct
     7021 tctgggacgc ctctcctgtg tgcctgagct tcatctaccc cctgtgcgcc cagctgaccg
     7081 gcctgaacct gtcttacgcc cctacactgg acgccagcga cctgaccaag atgatcagca
     7141 gatgcgtgaa gctccagaga ctgtgggtgc tggactgcat cagcgacaag ggactccagg
     7201 tggtggccag ctcctgcaag gatctccagg aactgagagt gttccccagc gacttctacg
     7261 tggccggcta cagcgccgtg accgaagagg gactggtggc tgtgtctctg ggctgcccca
     7321 agctgaacag cctgctgtac ttctgccacc agatgaccaa cgccgccctc gtgaccgtgg
     7381 ccaagaactg ccccaacttc accaggttca gactgtgcat cctggaaccc ggcaagcccg
     7441 acgttgtgac cagccagcca ctggatgagg gcttcggagc catcgtgcgc gagtgcaagg
     7501 gactccagag actgagcatc agcggcctgc tgaccgacaa ggtgttcatg tacatcggga
     7561 agtacgccaa gcagctggaa atgctgtcta tcgccttcgc tggcgactcc gacaagggca
     7621 tgatgcacgt gatgaacggc tgcaagaacc tgcggaaact ggaaatcaga gacagcccct
     7681 tcggcgacgc tgccctgctg ggcaacttcg ccagatacga gacaatgaga tccctgtgga
     7741 tgagcagctg caacgtgaca ctgaagggct gccaggtgct ggccagcaag atgcccatgc
     7801 tgaacgtgga agtgatcaac gagagggacg gcagcaatga gatggaagag aaccacggcg
     7861 acctgcctaa ggtggaaaag ctgtacgtgt acagaaccac agccggcgct agggacgacg
     7921 cccctaactt cgtgaagatc ctgggcggat cttacccata cgacgtacca gattacgctt
     7981 aggcgatcgc ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     8041 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     8101 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8161 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8221 acgcgtcgcc attttggcag ggaattctag aagttcctat actttctaga gaataggaac
     8281 ttcactcgac ctcgaaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     8341 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     8401 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     8461 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     8521 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     8581 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     8641 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     8701 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     8761 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc atccatctag
     8821 atctcgagca gctgaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     8881 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     8941 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     9001 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     9061 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     9121 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     9181 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     9241 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     9301 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     9361 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     9421 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
     9481 gcgcacgacc ccatgcatcg atgatatcag atccccggga tgcagaaatt gatgatctat
     9541 taaacaataa agatgtccac taaaatggaa gtttttcctg tcatactttg ttaagaaggg
     9601 tgagaacaga gtacctacat tttgaatgga aggattggag ctacgggggt gggggtgggg
     9661 tgggattaga taaatgcctg ctctttactg aaggctcttt actattgctt tatgataatg
     9721 tttcatagtt ggatatcata atttaaacaa gcaaaaccaa attaagggcc agctcattcc
     9781 tcccactcat gatctataga tctatagatc tctcgtggga tcattgtttt tctcttgatt
     9841 cccactttgt ggttctaagt actgtggttt ccaaatgtgt cagtttcata gcctgaagaa
     9901 cgagatcagc agcctctgtt ccacatacac ttcattctca gtattgtttt gccaagttct
     9961 aattccatca gaagctggtc gagatcctaa gcttggctgg acgtaaactc agaagttcct
    10021 atactttcta gagaatagga acttcaggta ccgcgggccc gggatcccca tatggtttct
    10081 gtgaaaatgc cttttcaatc attaacctct taaatgagtg ttagcatcct aactaatgag
    10141 tggtgcagaa tagtgggtct gcttagctta actaaggcca agaaaacaaa acaggaaatt
    10201 cattccatgt catgagactc atactacgag gttcccttag acctcaggag aaaaagtctt
    10261 tggctgtaag aacacacctc agtggatgtg gtagactatg cctttactct tttttttttt
    10321 tttttttttt tttgggtttt ttcgagacag ggtttctctg tgtagccttg gctgtcctgg
    10381 aactcacttt gttgaccagg ctggcctcga actcagagat ccgcctgcct ctgcctctca
    10441 agtgctagaa ttaaaagtgt gcgccaccac tcccagctat atagactatg cctttaatca
    10501 ggacacttgg gaggcagggg gatctcaaat tcaattccag acagatgact gacaaacaca
    10561 cacatacacc attcgtcttt tctttttttc ttttttcttt tgaaaacagg tttctctatg
    10621 cagccctggc tttcctagaa ttcaatctat agacccagct ggcctcaaac acagaacctc
    10681 ttgcctctgc ctcctcaaca ctatgactaa aggtgtgaac caccaccacc accacccacc
    10741 tggccaaaga atgaattgag tgaataagta cgagtgcaac tctccagggc cg
//
