LOCUS       CBS-1318_pBzCas13b-gRNA 4360 bp ds-DNA     circular     23-DEC-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(3076..3178)
                     /label="cat promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    228..257
                     /label="Spacer 3 BzCas13b"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    294..323
                     /label="Gibson homology R EcoRV pACYC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    192..227
                     /label="DR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        152..186
                     /label="J23119 promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(2416..3075)
                     /label="CmR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    258..293
                     /label="DR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2491..2491
                     /label="mismatch"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3704..4249
                     /label="p15A ori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 TTGACAGCTT ATCATCGATA AGCTTTAATG CGGTAGTTTA TCACAGTTAA ATTGCTAACG
       61 CAGTCAGGCA CCGTGTATGA AATCTAACAA TGCGCTCATC GTCATCCTCG GCACCGTCAC
      121 CCTGGATGCT GTAGGCATAG GCTTGGTTTA ATTGACAGCT AGCTCAGTCC TAGGTATAAT
      181 GCTAGCGCTG AGTTGGAACT GCTCTCATTT TGGAGGGTAA TCACAACCTC GATGTTGTGG
      241 CGGATCTTGA AGTTCACGTT GGAACTGCTC TCATTTTGGA GGGTAATCAC AACATCGTCC
      301 ATTCCGACAG CATCGCCAGT CACTATGGCG TGCTGCTAGC GCTATATGCG TTGATGCAAT
      361 TTCTATGCGC ACCCGTTCTC GGAGCACTGT CCGACCGCTT TGGCCGCCGC CCAGTCCTGC
      421 TCGCTTCGCT ACTTGGAGCC ACTATCGACT ACGCGATCAT GGCGACCACA CCCGTCCTGT
      481 GGATCCTCTA CGCCGGACGC ATCGTGGCCG GCATCACCGG CGCCACAGGT GCGGTTGCTG
      541 GCGCCTATAT CGCCGACATC ACCGATGGGG AAGATCGGGC TCGCCACTTC GGGCTCATGA
      601 GCGCTTGTTT CGGCGTGGGT ATGGTGGCAG GCCCCGTGGC CGGGGGACTG TTGGGCGCCA
      661 TCTCCTTGCA TGCACCATTC CTTGCGGCGG CGGTGCTCAA CGGCCTCAAC CTACTACTGG
      721 GCTGCTTCCT AATGCAGGAG TCGCATAAGG GAGAGCGTCG ACCGATGCCC TTGAGAGCCT
      781 TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC TATCGTCGCC GCACTTATGA
      841 CTGTCTTCTT TATCATGCAA CTCGTAGGAC AGGTGCCGGC AGCGCTCTGG GTCATTTTCG
      901 GCGAGGACCG CTTTCGCTGG AGCGCGACGA TGATCGGCCT GTCGCTTGCG GTATTCGGAA
      961 TCTTGCACGC CCTCGCTCAA GCCTTCGTCA CTGGTCCCGC CACCAAACGT TTCGGCGAGA
     1021 AGCAGGCCAT TATCGCCGGC ATGGCGGCCG ACGCGCTGGG CTACGTCTTG CTGGCGTTCG
     1081 CGACGCGAGG CTGGATGGCC TTCCCCATTA TGATTCTTCT CGCTTCCGGC GGCATCGGGA
     1141 TGCCCGCGTT GCAGGCCATG CTGTCCAGGC AGGTAGATGA CGACCATCAG GGACAGCTTC
     1201 AAGGATCGCT CGCGGCTCTT ACCAGCCTAA CTTCGATCAT TGGACCGCTG ATCGTCACGG
     1261 CGATTTATGC CGCCTCGGCG AGCACATGGA ACGGGTTGGC ATGGATTGTA GGCGCCGCCC
     1321 TATACCTTGT CTGCCTCCCC GCGTTGCGTC GCGGTGCATG GAGCCGGGCC ACCTCGACCT
     1381 GAATGGAAGC CGGCGGCACC TCGCTAACGG ATTCACCACT CCAAGAATTG GAGCCAATCA
     1441 ATTCTTGCGG AGAACTGTGA ATGCGCAAAC CAACCCTTGG CAGAACATAT CCATCGCGTC
     1501 CGCCATCTCC AGCAGCCGCA CGCGGCGCAT CTCGGGCAGC GTTGGGTCCT GGCCACGGGT
     1561 GCGCATGATC GTGCTCCTGT CGTTGAGGAC CCGGCTAGGC TGGCGGGGTT GCCTTACTGG
     1621 TTAGCAGAAT GAATCACCGA TACGCGAGCG AACGTGAAGC GACTGCTGCT GCAAAACGTC
     1681 TGCGACCTGA GCAACAACAT GAATGGTCTT CGGTTTCCGT GTTTCGTAAA GTCTGGAAAC
     1741 GCGGAAGTCC CCTACGTGCT GCTGAAGTTG CCCGCAACAG AGAGTGGAAC CAACCGGTGA
     1801 TACCACGATA CTATGACTGA GAGTCAACGC CATGAGCGGC CTCATTTCTT ATTCTGAGTT
     1861 ACAACAGTCC GCACCGCTGT CCGGTAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT
     1921 CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC CGGCAGCGCC
     1981 CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCCCTGCAC
     2041 CATTATGTTC CGGATCTGCA TCGCAGGATG CTGCTGGCTA CCCTGTGGAA CACCTACATC
     2101 TGTATTAACG AAGCGCTAAC CGTTTTTATC AGGCTCTGGG AGGCAGAATA AATGATCATA
     2161 TCGTCAATTA TTACCTCCAC GGGGAGAGCC TGAGCAAACT GGCCTCAGGC ATTTGAGAAG
     2221 CACACGGTCA CACTGCTTCC GGTAGTCAAT AAACCGGTAA ACCAGCAATA GACATAAGCG
     2281 GCTATTTAAC GACCCTGCCC TGAACCGACG ACCGGGTCGA ATTTGCTTTC GAATTTCTGC
     2341 CATTCATCCG CTTATTATCA CTTATTCAGG CGTAGCACCA GGCGTTTAAG GGCACCAATA
     2401 ACTGCCTTAA AAAAATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT
     2461 AAGCATTCTG CCGACATGGA AGCCATCACA GACGGCATGA TGAACCTGAA TCGCCAGCGG
     2521 CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA
     2581 GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA
     2641 GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA
     2701 CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA
     2761 GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC
     2821 CCATATCACC AGCTCACCGT CTTTCATTGC CATACGGAAT TCCGGATGAG CATTCATCAG
     2881 GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT
     2941 TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG
     3001 AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT
     3061 GATTTTTTTC TCCATTTTAG CTTCCTTAGC TCCTGAAAAT CTCGATAACT CAAAAAATAC
     3121 GCCCGGTAGT GATCTTATTT CATTATGGTG AAAGTTGGAA CCTCTTACGT GCCGATCAAC
     3181 GTCTCATTTT CGCCAAAAGT TGGCCCAGGG CTTCCCGGTA TCAACAGGGA CACCAGGATT
     3241 TATTTATTCT GCGAAGTGAT CTTCCGTCAC AGGTATTTAT TCGGCGCAAA GTGCGTCGGG
     3301 TGATGCTGCC AACTTACTGA TTTAGTGTAT GATGGTGTTT TTGAGGTGCT CCAGTGGCTT
     3361 CTGTTTCTAT CAGCTGTCCC TCCTGTTCAG CTACTGACGG GGTGGTGCGT AACGGCAAAA
     3421 GCACCGCCGG ACATCAGCGC TAGCGGAGTG TATACTGGCT TACTATGTTG GCACTGATGA
     3481 GGGTGTCAGT GAAGTGCTTC ATGTGGCAGG AGAAAAAAGG CTGCACCGGT GCGTCAGCAG
     3541 AATATGTGAT ACAGGATATA TTCCGCTTCC TCGCTCACTG ACTCGCTACG CTCGGTCGTT
     3601 CGACTGCGGC GAGCGGAAAT GGCTTACGAA CGGGGCGGAG ATTTCCTGGA AGATGCCAGG
     3661 AAGATACTTA ACAGGGAAGT GAGAGGGCCG CGGCAAAGCC GTTTTTCCAT AGGCTCCGCC
     3721 CCCCTGACAA GCATCACGAA ATCTGACGCT CAAATCAGTG GTGGCGAAAC CCGACAGGAC
     3781 TATAAAGATA CCAGGCGTTT CCCCCTGGCG GCTCCCTCGT GCGCTCTCCT GTTCCTGCCT
     3841 TTCGGTTTAC CGGTGTCATT CCGCTGTTAT GGCCGCGTTT GTCTCATTCC ACGCCTGACA
     3901 CTCAGTTCCG GGTAGGCAGT TCGCTCCAAG CTGGACTGTA TGCACGAACC CCCCGTTCAG
     3961 TCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGA AAGACATGCA
     4021 AAAGCACCAC TGGCAGCAGC CACTGGTAAT TGATTTAGAG GAGTTAGTCT TGAAGTCATG
     4081 CGCCGGTTAA GGCTAAACTG AAAGGACAAG TTTTGGTGAC TGCGCTCCTC CAAGCCAGTT
     4141 ACCTCGGTTC AAAGAGTTGG TAGCTCAGAG AACCTTCGAA AAACCGCCCT GCAAGGCGGT
     4201 TTTTTCGTTT TCAGAGCAAG AGATTACGCG CAGACCAAAA CGATCTCAAG AAGATCATCT
     4261 TATTAATCAG ATAAAATATT TCTAGATTTC AGTGCAATTT ATCTCTTCAA ATGTAGCACC
     4321 TGAAGTCAGC CCCATACGAT ATAAGTTGTA ATTCTCATGT
//