LOCUS       CBS-2261_pAcrVIA4       4341 bp ds-DNA     circular     22-DEC-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    4317..4341
                     /label="primer ML99 F"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(2329..2985)
                     /label="CAT/CmR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    72..302
                     /label="AcrVIA4"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      3858..4186
                     /label="p15A_ori"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    37..65
                     /label="UTR1"
                     /ApEinfo_revcolor=#5d67ff
                     /ApEinfo_fwdcolor=#5d67ff
     misc_feature    1333..1632
                     /label="f1 Origin"
                     /ApEinfo_revcolor=#0f770d
                     /ApEinfo_fwdcolor=#0f770d
     RBS             25..30
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     rep_origin      3347..3857
                     /label="p15A_ori"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    990..1274
                     /label="Δ[1-576]-Ampicillin"
                     /ApEinfo_revcolor=#ff1d00
                     /ApEinfo_fwdcolor=#ff1d00
     misc_feature    427..584
                     /label="rrnB_term"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    8..24
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    309..338
                     /label="T500 terminator"
                     /ApEinfo_revcolor=#ff1115
                     /ApEinfo_fwdcolor=#ff1115
     misc_feature    550..593
                     /label="rrnB_T1 term"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 gatgcattaa tacgactcac tataAGGAGG GCTAGCAATA ATTTTGTTTA ACTTTAAGAA
       61 GGAGAtatac cATGGATAAA GCGAACCGCT GCCTTAAAGC GAAGGACAAA ATTCTGAATA
      121 TTCTTGAAAA AGAAGAAATT ACTCTTGACG AATTTAATAA CATCAGCAAG GATATCGCAA
      181 AAGAATACGT TGAAAAAGCA GTTCTGAAGC CCAAGGATAT TGCAGAGCGT ATCATCAACA
      241 TGGTCAAAAA CGCTAAGTCT ATCAGTTTCG ATGAGCTGGC ATCAGAAATC TCCGAAGAGT
      301 AACTCGAGCA AAGCCCGCCG AAAGGCGGGC TTTTCTGTaa gcttggctgt tttggcggat
      361 gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
      421 agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag
      481 tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc
      541 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      601 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg
      661 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt
      721 aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt
      781 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      841 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      901 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      961 tgctgaagat cagttggggc aaactattat taccaatgct taatcagtga ggcacctatc
     1021 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1081 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1141 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1201 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1261 agtagttcgc cagtctgtca gaccaagttt actcatatat actttagatt gatttacgcg
     1321 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     1381 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     1441 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     1501 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     1561 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     1621 ttgttccaaa cttgaacaac actcaaccct atctcgggct attcttttga tttataaggg
     1681 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     1741 aattttaaca aaatattaac gtttacaatt taaaaggatc taggtgaaga tcctttttga
     1801 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1861 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1921 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1981 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     2041 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2101 aatcctgtta ccagtcaggc atttgagaag cacacggtca cactgcttcc ggtagtcaat
     2161 aaaccggtaa accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg
     2221 accgggtcga atttgctttc gaatttctgc cattcatccg cttattatca cttattcagg
     2281 cgtagcacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg
     2341 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     2401 gacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     2461 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     2521 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     2581 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     2641 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     2701 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     2761 catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     2821 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     2881 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     2941 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     3001 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     3061 aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg
     3121 cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac
     3181 aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc aacttactga tttagtgtat
     3241 gatggtgttt ttgaggtgct ccagtggctt ctgtttctat cagctgtccc tcctgttcag
     3301 ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc tagcggagtg
     3361 tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc atgtggcagg
     3421 agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata ttccgcttcc
     3481 tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa
     3541 cggggcggag atttcctgga agatgccagg aagatactta acagggaagt gagagggccg
     3601 cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct
     3661 caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg
     3721 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
     3781 ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag
     3841 ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat
     3901 cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat
     3961 tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag
     4021 ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag
     4081 aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg
     4141 cagaccaaaa cgatctcaag aagatcatct tattaatcag ataaaatatt tCTAGgctca
     4201 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     4261 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatctgct
     4321 catgtttgac agcttatcat c
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