LOCUS       Exported                8754 bp ds-DNA     circular SYN 21-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8754)
  AUTHORS   mao-s
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 22, 2020 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8754
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    205..221
                     /label=M13R
     misc_feature    complement(246..559)
                     /label=5'arm
     misc_feature    complement(246..556)
                     /label=5' TR long
                     /note="5' TR long"
     misc_feature    246..249
                     /label=New Feature(2)
     misc_feature    complement(248..282)
                     /label=5' TR
                     /note="5' TR"
     misc_feature    250..262
                     /label=13(1)
     misc_feature    266..284
                     /label=19
     misc_feature    complement(282..287)
                     /label=New Feature
     misc_feature    complement(377..406)
                     /label=SH-143
     misc_feature    complement(557..559)
                     /label=Core insulator
                     /note="Core insulator"
     misc_feature    568..1147
                     /label=CAG promoter
     promoter        568..1120
                     /label=CMV_immearly_promoter
     misc_feature    623..910
                     /label=CAG_enhancer
     promoter        1066..1147
                     /label=CMV_promoter
     misc_feature    1077..1097
                     /label=CMV_fwd_primer
     misc_feature    1125..1147
                     /label=SH-154
     misc_feature    1148..1153
                     /label=MluI
     misc_feature    1148..1150
                     /label=V5
     CDS             1160..1858
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             1868..1924
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     unsure          1911..1927
                     /label=left
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
     misc_feature    1928..2128
                     /label=PRKN
     misc_feature    2090..2092
                     /label=Feature 21
     misc_feature    2129..2145
                     /label=right
     misc_feature    2201..2917
                     /label=EGFP
     misc_feature    complement(2492..2511)
                     /label=AC_S54:EGFPRSeq
     misc_feature    complement(2492..2511)
                     /label=AC_S54:EGFPRSeq(1)
     misc_feature    2684..2704
                     /label=AC_S129:GFPFSeq
     misc_feature    complement(2785..2804)
                     /label=AC_S116:EGFPIntRSeq
     misc_feature    2918..2923
                     /label=AgeI
     misc_feature    2958..3183
                     /label=BGH polyA
     misc_feature    2967..2991
                     /label=New Feature(5)
     misc_feature    2967..2991
                     /label=430
     misc_feature    3065..3093
                     /label=New Feature(3)
     misc_feature    3191..3214
                     /label=Forward Primer
     misc_feature    3215..3248
                     /label=LoxP
     misc_feature    3249..3744
                     /label=PGK promoter
     misc_feature    3753..4556
                     /label=Neo
     misc_feature    4595..4791
                     /label=bpA
     misc_feature    4793..4826
                     /label=LoxP(1)
     misc_feature    4837..4857
                     /label=Reverse Primer
     misc_feature    complement(4866..5107)
                     /label=3'arm
     misc_feature    complement(4866..5107)
                     /label=3' TF long
                     /note="3' TF long"
     misc_feature    complement(5016..5045)
                     /label=SH-144
     misc_feature    complement(5041..5103)
                     /label=3' TF short
                     /note="3' TF short"
     misc_feature    5041..5059
                     /label=19(1)
     misc_feature    5056..5080
                     /label=New Feature(4)
     misc_feature    5056..5080
                     /label=199
     misc_feature    5091..5103
                     /label=13
     misc_feature    5104..5107
                     /label=New Feature(1)
     misc_feature    5118
                     /label=TOPO cloning site
     misc_feature    5214..5229
                     /label=M13F
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccttaac cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg
      301 ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc
      361 ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa
      421 cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc
      481 atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat
      541 tttcttgtta tagatatcat taattaaata gtaatcaatt acggggtcat tagttcatag
      601 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      661 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      721 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      781 tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc
      841 ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt
      901 attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata
      961 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     1021 ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca
     1081 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agaggtcgtt tagtgaaccg
     1141 tcagatcacg cgtgccacca tgagcgagct gattaaggag aacatgcaca tgaagctgta
     1201 catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc
     1261 ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc
     1321 cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca
     1381 gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac
     1441 cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg
     1501 cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca
     1561 gaagaaaaca ctcggctggg aggccttcac cgagacgctg taccccgctg acggcggcct
     1621 ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacat
     1681 caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta
     1741 tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca
     1801 cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaatgg
     1861 atccggcgca acaaacttct ctctgctgaa acaagccgga gatgtcgaag agaatcctgg
     1921 accggctgag caggctcgtt gggaagcagc ctccaaagaa accatcaaga aaaccaccaa
     1981 gccctgtccc cgctgccatg taccagtgga aaaaaatgga ggctgcatgc acatgaagtg
     2041 tccgcagccc cagtgcaggc tcgagtggtg ctggaactgt ggctgcgagt agaaccgcgt
     2101 ctgcatgggg gaccactggt tcgacgtgag cggttctggt gaagggcgag gttccttgct
     2161 gacctgtggc gatgtagaag aaaatcccgg ccccggatcc gtgagcaagg gcgaggagct
     2221 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     2281 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     2341 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     2401 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     2461 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     2521 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     2581 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     2641 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     2701 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     2761 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     2821 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     2881 cgggatcact ctcggcatgg acgagctgta caagtaaacc ggttaggggc ccgtttaaac
     2941 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3001 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     3061 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     3121 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     3181 ggcggcgcgt ccagtgtgct ggaattcggc ttcaacaact tcgtataatg tatgctatac
     3241 gaagttatca gtacttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     3301 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     3361 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     3421 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     3481 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     3541 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     3601 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     3661 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     3721 ctcatctccg ggcctttcga cctgcagcca atatgggatc ggccattgaa caagatggat
     3781 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     3841 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     3901 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     3961 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     4021 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     4081 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     4141 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     4201 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     4261 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga
     4321 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     4381 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     4441 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     4501 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagggg
     4561 atcaattctc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     4621 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     4681 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     4741 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg caacaacttc
     4801 gtataatgta tgctatacga agttatcagt acaagccgaa ttctgcagat atccatcggc
     4861 gcgcctaaaa gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa
     4921 ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata
     4981 ataaaactta atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca
     5041 catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta
     5101 gggttaaggc cggccaaaag ggcgaattct gcagatatcc atcacactgg cggccgctcg
     5161 agcatgcatc tagagggccc aattcgccct atagtgagtc gtattacaat tcactggccg
     5221 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     5281 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     5341 aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc
     5401 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5461 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5521 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5581 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5641 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5701 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5761 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaattc agggcgcaag
     5821 ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg
     5881 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag
     5941 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc
     6001 gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac
     6061 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag
     6121 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     6181 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     6241 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     6301 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     6361 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     6421 ttgggcgaag tgccggggca ggatctcctg tcatcccacc ttgctcctgc cgagaaagta
     6481 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     6541 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     6601 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     6661 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     6721 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     6781 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     6841 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     6901 atcgccttct atcgccttct tgacgagttc ttctgaattg aaaaaggaag agtatgagta
     6961 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7021 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7081 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7141 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7201 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7261 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7321 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7381 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7441 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7501 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7561 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7621 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7681 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7741 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7801 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7861 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7921 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7981 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8041 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8101 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8161 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8221 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8281 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8341 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8401 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8461 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8521 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8581 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8641 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8701 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//
