LOCUS       pANY8                   4157 bp    DNA     circular     10-JAN-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12072246|
COMMENT     VNTDBDATE|-12110150|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     misc_feature    complement(2610..3422)
                     /vntifkey="21"
                     /label=Kana
     D_segment       3528..4147
                     /vntifkey="7"
                     /label=pMB1
     RBS             192..1131
                     /vntifkey="32"
                     /label=ELP
     silencer        1182..1691
                     /vntifkey="37"
                     /label=Intern
     misc_feature    2054..2359
                     /vntifkey="21"
                     /label=ccdB
     misc_feature    118..135
                     /vntifkey="21"
                     /label=His-tag
     promoter        complement(2452..2498)
                     /vntifkey="30"
                     /label=T7-terminator
     LTR             46..68
                     /vntifkey="19"
                     /label=T7\Promoter
     misc_marker     2386..2423
                     /vntifkey="22"
                     /label=MCS-2
     insertion_seq   1700..1735
                     /vntifkey="14"
                     /label=MCS-1
BASE COUNT      946 a       984 c      1246 g       981 t 
ORIGIN
        1 ggttcctggc cttttgctgg cctttggtac cagatctcga tcccgcgaaa ttaatacgac 
       61 tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa ctttaagaag 
      121 gagatataca tatgggccac catcatcacc accatgatta tgatattcca actaccgaga 
      181 atttgtattt tcagggggtc ccggggaaag gcgtgcctgg tgtcggggtt ccaggtgtgg 
      241 gtgtaccagg ggtaggtgta ccaggtgtgg gcgttccagg cgttggtgta ccaggagtag 
      301 gagtcccagg tgtaggcgta ccgggttttg gagtacctgg aaagggcgtc ccaggggttg 
      361 gtgtacccgg cgttggtgtt cctggggtag gtgttccagg cgtaggagta ccaggcgttg 
      421 gagttcctgg tgttggggtg cctggcgttg gggttcctgg ttttggtgtc ccaggaaagg 
      481 gagttcctgg cgtaggcgtt ccaggagtcg gcgtaccggg agttggagtt ccaggagtag 
      541 gcgttcctgg agtcggagta cctggggtcg gcgtgcctgg agtcggtgtt ccaggattcg 
      601 gtgttcctgg taaaggcgtc ccaggagtgg gtgttcctgg cgtcggagtg cctggagtag 
      661 gggttcccgg tgtaggcgtg cccggagtag gtgtacctgg agttggtgta ccgggagtag 
      721 gtgtccctgg tttcggagtc cctggcaagg gggtacccgg agttggcgta ccgggggtcg 
      781 gggtcccagg cgtcggtgta ccgggcgttg gagtccccgg tgtcggtgtg cccggcgtcg 
      841 gcgtgcccgg tgtaggtgtg cctggtttcg gtgtgccagg caagggagta ccaggagtcg 
      901 gggtaccggg tgttggcgta cccggggtgg gggttcctgg agtgggagtt ccgggggtgg 
      961 gagtgcctgg ggtgggtgta ccgggggttg gggtgccagg attcggagtg ccggggaaag 
     1021 gtgttccggg tgtgggagtc cccggcgtag gggtacctgg tgtaggggta ccaggcgtag 
     1081 gtgtacccgg ggtcggagtt ccaggtgtag gtgtaccggg tgtcggggtc cccggattcg 
     1141 gtgtgagctc gaacaacaac aacaataaca ataacaacaa cgaattcgcc ctcgcagagg 
     1201 gcactcggat cttcgatccg gtcaccggta caacgcatcg catcgaggat gttgtcggtg 
     1261 ggcgcaagcc tattcatgtc gtggctgctg ccaaggacgg aacgctgcgt gcgcggcccg 
     1321 tggtgtcctg gttcgaccag ggaacgcggg atgtgatcgg gttgcggatc gccggtggcg 
     1381 ccatcctgtg ggcgacaccc gatcacaagg tgctgacaga gtacggctgg cgtgccgccg 
     1441 gggaactccg caagggagac agggtggcgc aaccgcgacg cttcgatgga ttcggtgaca 
     1501 gtgcgccgat tccggcgcgc gtgcaggcgc tcgcggatgc cctggatgac aaattcctgc 
     1561 acgacatgct ggcggaagaa ctccgctatt ccgtgatccg agaagtgctg ccaacgcggc 
     1621 gggcacggac gttcggcctc gaggtcgagg aactgcacac cctcgtcgcc gaaggggttg 
     1681 ttgtacacaa cggatccact agtaggcctg tcgacggtca gtccggctta ctaaaagcca 
     1741 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt 
     1801 atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga 
     1861 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc 
     1921 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag 
     1981 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac 
     2041 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct 
     2101 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct 
     2161 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat 
     2221 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat 
     2281 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct 
     2341 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtcga ctgaccgtca 
     2401 ggcctccatg ggagctcctg cagttggctg ctgccaccgc tgagcaataa ctagcataac 
     2461 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg 
     2521 gatatccctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga 
     2581 tgataagctg tcaaacatga gaattcttag aaaaactcat cgagcatcaa atgaaactgc 
     2641 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa 
     2701 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt 
     2761 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca 
     2821 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag tttgtgcatt 
     2881 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca 
     2941 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta 
     3001 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca 
     3061 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg 
     3121 atcgcagtgg tgagtaacca tgcgtcatca ggagtacgga taaaatgctt gatggtcgga 
     3181 acaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca 
     3241 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga 
     3301 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca 
     3361 gcatccatgt tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc 
     3421 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgaccaaaat 
     3481 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 
     3541 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 
     3601 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 
     3661 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 
     3721 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 
     3781 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 
     3841 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 
     3901 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 
     3961 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 
     4021 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 
     4081 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 
     4141 caacgcggcc tttttac 
//
